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  • gene expression
  • Despite the preferential association of K4-trimethylated H3 with regions of the genome that are transcribed by RNA polymerase II, transcriptional silencing is one of the few cases in S. cerevisiae where histone-methylation defects have a clear effect on gene expression. (genetics.org)
  • To complement gene-expression analysis, quantitative Western blot experiments were performed to determine the members of COMPASS that are required for methylation of histone H3. (genetics.org)
  • B) Correlation between the level of Abcc6 gene expression (% of liver expression) and DNA methylation. (nih.gov)
  • Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. (osti.gov)
  • This project aimed to investigate the effects of high dietary n-6 PUFAs, particularly arachidonic acid (ARA), on parental metabolic profiles and on both hepatic gene expression and DNA methylation profiles in the first and following generation of zebrafish (Danio rerio). (uib.no)
  • Diet associated changes in the gene expression and DNA methylation profiles in adult male F0 and F1 livers were investigated using RNAsequencing and reduced representation bisulfite sequencing, respectively. (uib.no)
  • Changes in liver DNA methylation and gene expression profiles in adult male progeny were associated with the parental diet, whereas the effect on gene expression was less strong in parents than in progeny. (uib.no)
  • Several links were found between the metabolic profiles in parents and both DNA methylation and gene expression of the progeny, which suggested an impact of parental diet on the progeny perhaps during early embryonic development. (uib.no)
  • defects
  • Several genetic diseases are caused by defects within the methylation machinery, like the Rett Syndrome, Fragile X Syndrome and ICF (Immunodeficiency-Centromeric Instability-Facial Anomalies Syndrome). (sabiosciences.com)
  • The growth disorders Beckwith-Wiedemann syndrome and Silver-Russell syndrome are linked to opposite DNA methylation defects involving the ICR1. (biomedcentral.com)
  • regulators
  • Furthermore, DNA response elements can exert allosteric effects on transcriptional regulators, such that regulators may activate transcription in the context of one gene, yet repress transcription in another (Ref.1). (sabiosciences.com)
  • We observed that sperm from LPD-fed male mice displayed global hypomethylation associated with reduced testicular expression of DNA methylation and folate-cycle regulators compared with normal protein diet (NPD) fed males. (pnas.org)
  • intragenic
  • DNA methylation of intragenic CpG islands depends on their transcriptional activity during differentiation and disease. (ox.ac.uk)
  • Importantly, we also show that a subgroup of intragenic CGIs is not sensitive to this process of transcription-mediated methylation and that this correlates with their individual intrinsic capacity to initiate transcription in vivo. (ox.ac.uk)
  • The ESR1 promoter was unmethylated in breast tumour biopsies with high levels of intragenic methylation, independent of ER status. (beds.ac.uk)
  • DAC treatment inhibited ESR1 transcription in cells with an unmethylated ESR1 promoter and reduced intragenic DNA methylation. (beds.ac.uk)
  • Intragenic methylation levels correlated with ESR1 expression in homogenous cell populations (cell lines and exfoliated primary breast epithelial cells), but not in heterogeneous tumour biopsies, highlighting the significant differences between the in vivo tumour microenvironment and individual homogenous cell types. (beds.ac.uk)
  • metabolism
  • Reactions in the folate-mediated one-carbon metabolism pathway are linked to DNA methylation and to other vital cell functions. (nih.gov)
  • Genetic components of the folate-mediated one-carbon metabolism pathway can affect DNA methylation and other vital cell functions, including DNA synthesis, amino acid biosynthesis, and cell growth. (biomedcentral.com)
  • DNA methylation processes are also affected by reactions in the folate-mediated one-carbon metabolism (OCM) pathway. (biomedcentral.com)
  • demethylation
  • These transcriptional changes are likely to influence vital cellular functions of DNA methylation and demethylation, cellular growth, DNA biosynthesis, and DNA repair, and some of them may contribute to cytotoxic and apoptotic action of the drugs. (biomedcentral.com)
  • machinery
  • Dogma states that methylation of the promoter region-associated CGIs leads to conformational changes in the DNA strand and regional chromatin [ 5 , 6 ], which inhibit the initiation of the transcriptional machinery and prevent the recruitment of RNA polymerase II. (beds.ac.uk)
  • DNMT3b
  • Methylation at CpG dinucleotides is carried out by one of the three known human DNA methyltransferases (DNMT1, DNMT3a and DNMT3b), resulting in DNA with high levels of CpG methylation, but still containing predominantly acetylated histone tails. (sabiosciences.com)
  • initiation
  • Our results suggest that in the inhibition of vulval development, homologs of SETDB1, HP1 and the NuRD complex act with this H3K36 HMT to prevent ectopic transcriptional initiation. (biologists.org)
  • role
  • To better understand the role of COMPASS in transcriptional silencing, we have determined which members of COMPASS are required for silencing at the ribosomal DNA locus (rDNA), a telomere, and the silent mating loci ( HM ) using Northern analyses. (genetics.org)
  • However, the role of gene body methylation remains largely unresolved. (biomedcentral.com)
  • Nevertheless, the main role of gene body DNA methylation remains unresolved. (biomedcentral.com)
  • Methylation associated with PTGS in plants is acquired in transcribed or coding regions, where its role is still unclear. (embopress.org)
  • The role of Kaiso-ICR1 binding on DNA methylation was tested by lentiviral Kaiso knockdown and CRISPR/Cas9 mediated editing of a Kaiso binding site. (biomedcentral.com)
  • This method provides the opportunity to use publicly available DNA methylation datasets, more widely available than Hi-C data, to investigate the role of chromatin compartmentalization in human diseases, potentially highlighting previously unknown molecular characteristics that could pave the way to novel therapeutics. (biomedcentral.com)