Nrf2RegulatorRegulationGlutathioneElectrophile responseInductionResponsiveEnzymesReactiveProteinProtective2021ApoptosisTarget genesActivationEndogenousLuciferaseBiochemistryMechanismDepletionFunctionalOxidative damageMolecularActivityImmuneFactorConcentration-dependentLevelTissuesStressInflammationImportantBiological
Nrf229
- The latter DNA sequences have been recognized as antioxidant/electrophile response elements or NF-E2-binding motifs to which Nrf2-sMaf heterodimers and p45 NF-E2-sMaf heterodimer bind, respectively. (wikipedia.org)
- Nrf2-sMaf heterodimer regulates a battery of cytoprotective genes, such as antioxidant/xenobiotic metabolizing enzyme genes. (wikipedia.org)
- Mafk−/− mice fail to activate Nrf2-dependent cytoprotective genes in response to stress. (wikipedia.org)
- 2012. Cap-independent Nrf2 translation is part of a lipoic acid-stimulated detoxification stress response. . (oregonstate.edu)
- 2004. Decline in transcriptional activity of Nrf2 causes age-related loss of glutathione synthesis, which is reversible with lipoic acid. . (oregonstate.edu)
- Thus, medicarpin might protect cells by promoting the NRF2 transcriptional activity. (gnu.ac.kr)
- Specifically, both MRE activation by MTF-1 and that of ARE in the promoter region of the MT-2A gene by Nrf2 are involved in transcriptional induction of MT-1A , whereas only MRE activation by MTF-1 or other transcriptional factor(s) is required for transcriptional induction of MT-2A in vascular endothelial cells. (go.jp)
- Here, we hypothesized that multiple transcriptional regulators, including: (i) a primary Nrf2-Maf heterodimer, (ii) a proposed secondary, EpRE-specific, p160 family coactivator, ARE-binding protein-1, and (iii) a tertiary coactivator, CBP/p300, nucleate to form a complex at the EpRE that regulates transcription of the dependent gene. (nih.gov)
- Når de aktive substanser når cellens kerne via aktivering af Nrf2, starter kroppens egne forsvarsmekanismer - og det sker på en måde, som kun visse naturligt forekommende stoffer er i stand til. (cognicore.net)
- Fumarate is cardioprotective via activation of the Nrf2 antioxidant pathway. (ox.ac.uk)
- In addition, by stabilizing the transcriptional regulator Nrf2, Fh1 KO hearts upregulate protective antioxidant response element genes. (ox.ac.uk)
- The discovery of the antioxidant response element (ARE) have led to the conclusion that the battery of genes, including glutamate-cysteine ligase (GCL), thioredoxin reductase 1 (Txnrd1), NAD(P)H-quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HMOX1) is regulated through Nrf2 binding to this consensus binding sequence [ 3 ]. (springer.com)
- While the expression of phase II enzymes (e.g., glutathione S-transferase and NAD(P)H: quinone oxidoreductase) was markedly induced by a phenolic antioxidant in vivo in both wild type and heterozygous mutant mice, the induction was largely eliminated in the liver and intestine of homozygous nrf2-mutant mice. (elsevierpure.com)
- These results demonstrate that Nrf2 is essential for the transcriptional induction of phase II enzymes and the presence of a coordinate transcriptional regulatory mechanism for phase II enzyme genes. (elsevierpure.com)
- Induction of the genes required antioxidant response elements (ARE) as Cd drove ARE-dependent reporter expression and Cd-activated Nrf2 bound to endogenous AREs in mouse hepa1c1c7 cells. (cdc.gov)
- The study provided a mechanistic transcriptional model in which Cd activates Nrf2 through a metal-activated signaling pathway involving a dynamic interplay between ubiquitination/deubiquitination and complex formation/dissociation of Nrf2 and Keap1. (cdc.gov)
- Exposure of rodents to 1,2-dithiole-3-thiones triggers nuclear accumulation of the transcription factor Nrf2 and its enhanced binding to the "antioxidant response element" (ARE), leading to transcriptional activation of a score of genes involved in carcinogen detoxication and attenuation of oxidative stress. (johnshopkins.edu)
- The nuclear factor erythroid-derived two-like 2-antioxidant response element (Nrf2-ARE) pathway and its downstream antioxidant enzyme heme oxygenase-1 (HMOX1 or HO-1) play essential roles in H2 O2 -induced oxidative damage in human melanocytes. (nih.gov)
- We subsequently found that Nrf2 rs35652124 C allele had higher transcriptional activity in the luciferase reporter assay compared with Nrf2 rs35652124 T allele. (nih.gov)
- One of these transcriptional regulators is nuclear factor erythroid 2-related factor 2, or NRF2, identified in 1994 by another research group. (asbmb.org)
- Because NRF2 binds ARE, suggesting it helps regulate oxidative stress responses, Pickett and his team focused on it next and helped uncover key aspects of how NRF2 itself is regulated. (asbmb.org)
- Nrf2 is a key transcription aspect for genes coding for antioxidants, cleansing enzymes, and multiple medication level of resistance and it confers level of resistance to anticancer medications also. (researchtoactionforum.org)
- Cox regression evaluation showed that sufferers with high-Nrf2, high-Bcl-2, high-Bcl-xL mRNA tumors had been even more occurred unfavorable response to cisplatin-based chemotherapy than their counterparts typically. (researchtoactionforum.org)
- Collectively, mutant p53 might confer cisplatin level of resistance via upregulation of Nrf2 appearance, and Nrf2 mRNA level may predict chemotherapeutic outcomes and response in NSCLC. (researchtoactionforum.org)
- Gene appearance of antioxidants that remove ROS is marketed by an NF-E2-related aspect 2 (Nrf2), which binds to antioxidant response components (ARE) from the promoters of the genes [7C11]. (researchtoactionforum.org)
- Mutant k-ras confers chemoresistance by upregulating Nrf2 transcription through a TPA response element [24]. (researchtoactionforum.org)
- These total outcomes claim that Nrf2 appearance may be suppressed by wild-type p53, however, not by mutant p53 at transcriptional level. (researchtoactionforum.org)
- OBJECTIVE- Sulforaphane is an activator of transcription factor NF-E2-related factor-2 (nrf2) that regulates gene expression through the promoter antioxidant response element (ARE). (diabetesjournals.org)
- CONCLUSIONS- We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role. (diabetesjournals.org)
Regulator2
- A basic-leucine zipper transcription factor that was originally described as a transcriptional regulator controlling expression of the BETA-GLOBIN gene. (nih.gov)
- These genes can be generally classified into two main groups based on their products, effector molecules or regulator molecules, which are also correspondingly designated as delayed-response genes or early-response genes based on their response timing [ 4 - 6 ]. (biomedcentral.com)
Regulation7
- The contribution of individual sMafs to the transcriptional regulation of their target genes has not yet been well examined. (wikipedia.org)
- 2009. Transcriptional regulation of rat gamma-glutamate cysteine ligase catalytic subunit gene is mediated through a distal antioxidant response element. . (oregonstate.edu)
- Current models of transcription regulation have also shown the involvement of higher-order transcriptional coactivators, proteins that nucleate around DNA sequence-specific transcription factors, enhancing transcription of the target gene by interacting with components of the basal transcriptional apparatus and by enabling chromatin remodeling. (nih.gov)
- To identify the involvement of specific, primary and higher-order transcriptional regulators in the EpRE-mediated regulation of the GFP reporter gene, we microinjected antibodies directed against specific transcription factors into the HepG2/GFP cells and determined their effect upon tBHQ-induced expression of the GFP gene. (nih.gov)
- encoded by Nfe2l2 gene) is a transcription factor responsible for the regulation of cellular redox balance and protective antioxidant and phase II detoxification responses in mammals [ 1 , 2 ]. (springer.com)
- ABA-responsive element (ABRE, PyACGTGGC), an important cis -element, is implicated in the transcriptional regulation of ABA- and/or stress-responsive genes [ 8 ]. (biomedcentral.com)
- His Regulation of gene expression research incorporates elements of Protease, Mutant, Proteasome, Kelch-Like ECH-Associated Protein 1 and Protein biosynthesis. (research.com)
Glutathione3
- Photoperiod (either 12 light:12 dark cycle, LD or 0 light:24 dark cycle, DD) during explant culture affected the transcription of two clock genes, circadian locomotor output cycles kaput (clk) and period 1 (per1), as well as one antioxidant gene, glutathione peroxidase (gpx). (nofima.no)
- These antioxidant enzymes include glutathione S -transferase, or GST, NADPH:quinone oxidoreductase 1, thioredoxin and hemeoxygenase-1. (asbmb.org)
- Ferroptosis-inducing factors can directly or indirectly affect glutathione peroxidase through different pathways, resulting in a decrease in antioxidant capacity and accumulation of lipid reactive oxygen species (ROS) in cells, ultimately leading to oxidative cell death. (nature.com)
Electrophile response1
- The electrophile response element (EpRE), also referred to as the antioxidant responsive element (ARE), is found in the 5'-regulatory region of a number of genes encoding phase II, drug-metabolizing enzymes. (nih.gov)
Induction4
- We propose potential mechanisms underlying transcriptional induction of endothelial MT isoforms. (go.jp)
- A short-term gill explant (GE) culture was used as a model in a series of experiments aiming to demonstrate how photoperiod during culture, levels of environmental reactive oxygen species (ROS), time of oxidative stress induction, and the daily light-dark cycle affect the expression of molecular clocks and antioxidant genes in the gills. (nofima.no)
- To test the hypothesis that enzyme induction is a useful strategy for chemoprevention in humans, three key elements are necessary: a candidate agent, an at-risk population and modulatable intermediate endpoints. (johnshopkins.edu)
- Mechanistically, MTX treatment led to cyclic AMP response element-binding protein (CREB) Ser133 phosphorylation, while AMPK depletion attenuated this response and the induction of MnSOD and HO-1. (bmj.com)
Responsive3
- Their work made it clear that antioxidant genes such as GST have multiple regulatory elements in their promoters that are responsive to specific cellular stressors. (asbmb.org)
- ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes. (biomedcentral.com)
- We identified and validated the transcription factor cAMP-responsive element binding protein (Creb1) and its transcriptional co-Activators (Crtc1-3) as targets of miR-17, miR-144, and miR-21. (edu.au)
Enzymes2
- While this group of enzymes is believed to be under the transcriptional control of antioxidant response elements (AREs), this contention is experimentally unconfirmed. (elsevierpure.com)
- Under dehydration and drought conditions, the transgenic plants accumulated lower levels of reactive oxygen species compared with wild type, accompanied by higher activities and expression levels of three antioxidant enzymes. (biomedcentral.com)
Reactive1
- Here, we demonstrate that OP2113 (5-(4-Methoxyphenyl)-3H-1,2-dithiole-3-thione, CAS 532-11-6), synthesized and used as a drug since 1696, does not act as an unspecific antioxidant molecule (i.e., as a radical scavenger) but unexpectedly decreases mitochondrial reactive oxygen species (ROS/H2O2) production by acting as a specific inhibitor of ROS production at the IQ site of complex I of the mitochondrial respiratory chain. (epiphanyasd.com)
Protein4
- The antioxidant response element, or ARE, present in the promoters of many antioxidant protein genes is a major regulatory lynchpin in this cellular response. (asbmb.org)
- Transfer RNAs (tRNAs) enter ribosome-mediated protein biosynthesis in a translationally competent state, which includes post-transcriptional modifications at various positions, including the anticodon loop, and the presence of an intact single-stranded CCA-sequence at the 3′-terminus that is required for amino acid attachment by the corresponding aminoacyl-tRNA-synthetase [7] . (prolekarniky.cz)
- Recent studies indicate that nutrient fluctuations and insulin resistance increase proinsulin synthesis in β cells beyond the capacity for folding of nascent polypeptides within the endoplasmic reticulum (ER) lumen, thereby disrupting ER homeostasis and triggering the unfolded protein response (UPR). (jci.org)
- His work carried out in the field of Transcription factor brings together such families of science as Ubiquitin, Antioxidant, Cycloheximide and Protein kinase A, p38 mitogen-activated protein kinases. (research.com)
Protective2
- Hormesis is a process through which moderate stress induces a body response that is protective against insults, confers health and possibly even longevity benefits. (anti-agingfirewalls.com)
- The protective effects are mediated by transcriptional responses to restore cellular homeostasis. (uef.fi)
20215
- 2021) Nitric oxide coordinates growth, development, and stress response via histone modification and gene expression. (oeaw.ac.at)
- 2021) A genome-wide association study unravels cytokinin as a major component in the root defense responses against Ralstonia solanacearum. (oeaw.ac.at)
- 2021) 1,135 ionomes reveals the global pattern of leaf and seed mineral nutrient and trace element diversity in Arabidopsis thaliana. (oeaw.ac.at)
- 2021) A complex immune response to flagellin epitope variation in commensal communities. (oeaw.ac.at)
- Déléris A, Berger F, Duharcourt S (2021) Role of Polycomb in the control of transposable elements. (oeaw.ac.at)
Apoptosis1
- EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
Target genes2
- TF constitutes the key regulon that plays crucial roles in various biological processes, including stress response, through binding to the cis -acting element in the promoters of target genes [ 7 ]. (biomedcentral.com)
- TFs are known as master switch in plant stress response, and genetic transformation of the genes encoding TFs has been suggested as a possible approach for engineering stress tolerance as manipulation of a single TF can alter expression of a wide array of target genes [ 7 ]. (biomedcentral.com)
Activation1
- Similar to other sMafs, MafK lacks any canonical transcriptional activation domains. (wikipedia.org)
Endogenous3
- Evidence for its initiation of stress signaling pathways that promote endogenous antioxidant capacity. (oregonstate.edu)
- This interaction increases endogenous production of antioxidants, local perfusion, and oxygen delivery, as well as enhances immune responses. (diegovitello.it)
- molecules to create more active substrates, thus stimulating an endogenous cascade of responses. (diegovitello.it)
Luciferase2
- The plasmid contains a luciferase gene (reporter gene) which is under transcriptional control of an antioxidant response element (ARE) of the rat NQO1 gene. (europa.eu)
- Medicarpin significantly induced the antioxidant response elements (ARE)-luciferase activity in a concentration-dependent manner. (gnu.ac.kr)
Biochemistry1
- His Biochemistry research is multidisciplinary, incorporating elements of Endocrinology and Allergic inflammation. (research.com)
Mechanism1
- The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
Depletion1
- 2005. Dietary supplementation with (R)-alpha-lipoic acid reverses the age-related accumulation of iron and depletion of antioxidants in the rat cerebral cortex. . (oregonstate.edu)
Functional1
- A hormetic response to a stress not only maintains a functional internal environment but also improves it. (anti-agingfirewalls.com)
Oxidative damage2
- Exposure to cadmium (Cd) elicits a range of adverse responses including oxidative damage and cancer. (cdc.gov)
- In addition, we found that isolated islets from Chop -/- mice displayed increased expression of UPR and oxidative stress response genes and reduced levels of oxidative damage. (jci.org)
Molecular4
- Molecular clocks are known to mediate cellular responses during oxidative stress. (nofima.no)
- Here we report the coordinated modulation of the molecular clocks and antioxidant defence following chemically induced oxidative stress in the gill mucosa of Atlantic salmon (Salmo salar). (nofima.no)
- Collectively, the study revealed the coordinated expression of the core elements in the molecular clock and antioxidant systems in the gill mucosa following oxidative stress. (nofima.no)
- Standard molecular typing methods such as multilo- elements. (cdc.gov)
Activity2
- To examine the antioxidant activity of EESR, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay was performed. (jcpjournal.org)
- EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
Immune1
- They occasionally exhibit toxic, mutagenic or carcinogenic effects, or they modulate immune responses. (cdc.gov)
Factor1
- These responses were preserved when HUVECs were pretreated with tumour necrosis factor-α to mimic dysfunctional endothelium. (bmj.com)
Concentration-dependent1
- The concentration-dependent responses of the test plants to the fractions suggested that allthree fractions might contain allelochemicals, but that the greatest potential was in the water-soluble fraction. (researchgate.net)
Level2
- The vertical axis represents relative risk, level of probable pathological organism response where normal level is 1. (anti-agingfirewalls.com)
- On the other hand, photoperiod was shown to influence the antioxidant defence under increased ROS level, where significant transcriptional upregulation was a hallmark response under LD. (nofima.no)
Tissues1
- Furthermore, the results reveal that the time of day plays a crucial influence on how defences are mobilised during oxidative stress, adding new insights into the rhythms of oxidative stress response in mucosal tissues in fish. (nofima.no)
Stress2
- Subsequent work by Pickett's lab and others helped identify the transcription factors that recognize the ARE and activate the transcription of GST and other oxidative stress response genes. (asbmb.org)
- Translation initiation, as a rate-limiting process, is a major point to reprogram translation in response to stress [3] , [4] . (prolekarniky.cz)
Inflammation2
- The inflammation and coagulation response after ICH would accelerate the formation of brain edema around hematoma, resulting in a more severe and durable injury. (karger.com)
- Besides the importance of this process, oxidation of LDL is not the sole initiator of inflammation, as the imbalance between oxidants and antioxidants is also important for the process of atherogenesis. (hindawi.com)
Important1
- Finally, we propose that Creb1/Crtc1-3 and Sec14l3 could be important for early responses of the bronchial epithelium to Th2-stimuli. (edu.au)
Biological1
- The physiology of these biological responses is herein discussed. (diegovitello.it)