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  • nascent
  • Rho binds to the nascent RNA and translocates 5′ to 3′ along RNA using energy derived from ATP hydrolysis ( 4 ). (pnas.org)
  • Latter, the molecular structure of the elongating RNA Pol II ensures that the nascent transcript leaves the transcription bubble physically separated from the template DNA. (ul.pt)
  • Additionally, various RNA processing factors are also known to prevent R-loop formation by sequestering the nascent RNA, thus preventing it from re-annealing to the template DNA. (ul.pt)
  • ChIP-Seq demonstrates AGO2 association with a large number of ERβ binding sites, and total and nascent RNA-Seq in ERβ + vs ERβ − cells, and before and after AGO2 knock-down in ERβ + cells, reveals a widespread involvement of this factor in ERβ-mediated regulation of gene transcription rate and RNA splicing. (beds.ac.uk)
  • occupancy
  • S2 and/or tyrosine 1 (Y1) phosphorylation of the CTD is required for TREX occupancy in vivo, establishing a second interaction platform necessary for TREX recruitment in addition to RNA. (prolekare.cz)
  • methylation
  • Epigenetic dysregulation in B cells, including the aberrant expression of non-coding RNAs and alterations of histone modifications and DNA methylation, can result in aberrant antibody responses to foreign antigens, such as those on microbial pathogens, and generation of pathogenic autoantibodies, IgE in allergic reactions, as well as B cell neoplasia. (frontiersin.org)
  • genome-wide
  • In the course of this work, we have optimized the RNA-Seq protocol for use with trypanosomatids, built our own server, implemented a stream-lined bioinformatics pipeline and generated one of the most comprehensive genome-wide transcript maps at single-nucleotide resolution. (yale.edu)
  • cytoplasm
  • Also acts as an attachment signal for the small "ribosomal subunit" in the cytoplasm. (richardsonthebrain.com)
  • ERβ-AGO2 association was confirmed in vitro and in vivo in both the nucleus and cytoplasm and is shown to be RNA-mediated. (beds.ac.uk)
  • The argonaute protein AGO2 is a RNA-binding protein primarily known for its functions in the cytoplasm, where it is a major component of the RNA-induced silencing complex (RISC). (beds.ac.uk)
  • Trypanosoma
  • Most recently, we have established and used RNA-sequencing (RNA-Seq), a novel high-throughput sequencing technology, to interrogate the transcriptome of the parasitic protozoa Trypanosoma brucei , the agent of African sleeping sickness in man and nagana in cattle. (yale.edu)
  • Developmental progression to infectivity in Trypanosoma brucei triggered by an RNA-binding protein. (yale.edu)
  • transcriptional
  • Co-transcriptional R-loops impact on RNA Pol II transcription dynamics. (ul.pt)
  • Nucleocytoplasmic shuttling is a specific property also of estrogen receptor β (ERβ) [ 14 , 15 ], a member of the nuclear receptor superfamily of transcriptional regulators [ 16 ] that shows oncosuppressive activities in BC and other cancers. (beds.ac.uk)
  • biological
  • In total, five biological replicates representing the differentiation of matched parasite populations derived from five individual mouse infections were assayed, with RNAs being derived at key biological time points during the time course of their synchronous differentiation to procyclic forms. (biomedcentral.com)
  • We made a series of discoveries that introduced the important and pervasive roles of long noncoding RNAs in biological regulation. (stanford.edu)
  • RNAs are now known to adopt complex tertiary structures and act as biological catalysts. (news-medical.net)
  • gene regulation
  • Applying interaction proteomics coupled to mass spectrometry to characterize nuclear factors cooperating with ERβ in gene regulation, we identify AGO2 as a novel partner of ERβ in human BC cells. (beds.ac.uk)
  • strands
  • These are called dsRNA where the RNA has two complementary strands, similar to the DNA found in all cells. (news-medical.net)
  • mechanisms
  • Thus, 7SK uses distinct mechanisms to counteract the diverse consequences of pervasive transcription that distinguish super enhancers, enhancers and promoters. (nih.gov)
  • Drosophila
  • A small system--high-resolution study of metabolic adaptation in the central metabolic pathway to temperate climates in Drosophila melanogaster. (sdbonline.org)
  • abundant
  • Actin is abundant in the nucleus and it is clear that nuclear actin has important functions. (biologists.org)
  • However, the past decade of research has shown those R-loops − RNA-DNA hybrids and a displaced single-stranded DNA−are abundant structures that play important roles in regulating gene expression and DNA recombination. (ul.pt)
  • 7SK RNA: 7SK was discovered as an abundant small nuclear RNA in the mid-70s but a possible function has only recently been suggested . (genecards.org)
  • isolation
  • All cell lines were transfected at approximately 80-90% confluencey in 8-well chamber slides (Nunc) for fluorescence microscopy, 24-well plates for flow cytometry analysis or 25 cm2 culture flask for RNA isolation. (alliedacademies.org)
  • structures
  • In addition, lncRNAs, such as recently reported lncRNA-CSR and an lncRNA generated through transcription of the S region that form G-quadruplex structures, are also important for CSR targeting. (frontiersin.org)
  • Biochemical
  • For these factor characterizations we plan to employ a plethora of genetic and biochemical experiments which include tandem affinity purification of protein complexes, conditional gene silencing experiments and in vitro transcription assays. (grantome.com)