• Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
  • Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
  • The Brn-3b transcription factor regulates the growth, behavior, and invasiveness of human neuroblastoma cells in vitro and in vivo. (ox.ac.uk)
  • Our results therefore suggest that Brn-3b transcription factor contributes to proliferation of neuroblastoma cells in vivo and in vitro but may also influence progression and/or invasion during tumorigenesis. (ox.ac.uk)
  • A composite model of KEOPS bound to tRNA reveals that all KEOPS subunits form an extended tRNA-binding surface that we have validated in vitro and in vivo to mediate the interaction with the tRNA substrate and its modification. (rcsb.org)
  • In vivo and in vitro inflammatory responses to fine particulate matter (PM2.5) from China and California. (cdc.gov)
  • Time-lag experiments were performed to explore the in vivo and in vitro toxicity of the PM extracts. (cdc.gov)
  • Some biologic features plaque assay, we determined that approximately 360 viral of the SARS-CoV described in vivo and in vitro differ genomes were required to generate a PFU. (cdc.gov)
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
  • Purified sgRNA from this kit is ready for direct use in transfection, electroporation, or in vitro cleavage assays. (takarabio.com)
  • Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
  • We have used block-substitution mutagenesis and in vitro transcription assays to identify cis-acting DNA sequence elements important for initiation and 3'-end formation of a U1 small nuclear RNA (snRNA) in the parasitic nematode Ascaris lumbricoides. (cshl.edu)
  • Luciferase reporter and quantitative polymerase chain reaction assays were used to determine in vitro effects on aryl hydrocarbon receptor (AhR) activation and gene transcription, respectively. (cdc.gov)
  • Considering this peculiar aspect of trypanosomatid mRNA, the aim of the present work was to develop a Trypanosoma cruzi specific in vitro transcription (IVT) linear mRNA amplification method in order to improve parasite transcriptomics analyses. (altmetric.com)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • In the dipteran Drosophila melanogaster , TGFbeta signaling stimulates JH production by up-regulating the transcription of the JH acid methyltransferase (JHAMT ) gene through the transcription factor Mothers against Dpp (Mad) 14 . (nature.com)
  • Transcription factor EB (TFEB)-mediated autophagy is an important cytoprotective mechanism against oxidative stress. (illinois.edu)
  • Although the TATA element-binding factor TFIID has been purified and cloned from several organisms and has provided invaluable insight into the process of transcription initiation and its regulation, little is known about factors that interact at the +1 region. (princeton.edu)
  • We have recently shown that the adeno-associated virus type 2 P5 promoter +1 region (P5 + 1 element) binds transcription factor YY1 (ref. 5). (princeton.edu)
  • We previously showed that the Brn-3b transcription factor is highly expressed in actively proliferating neuroblastoma cells but is significantly decreased when these cells are induced to differentiate. (ox.ac.uk)
  • Up-stream transcription factor analysis indicated activation of several lipid-related transcription regulators (e.g. (oregonstate.edu)
  • Induction of autophagy is tightly regulated at the transcriptional level and involves the master regulator, transcription factor EB (TFEB). (biomedcentral.com)
  • Transcription factor Sp2 promotes TGFB-mediated interstitial cell osteogenic differentiation in bicuspid aortic valves through a SMAD-dependent pathway. (nih.gov)
  • Transcription Factor SP2 Regulates Ski-mediated Astrocyte Proliferation In Vitro. (nih.gov)
  • As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • For short templates we recommend to increase in template concentration, polymerase concentration and reaction time, since transcription of small RNAs is largely dependent on the number of initiation events. (neb.com)
  • In addition, inflammation‑inflicted oxidative stress was induced in vitro by stimulating chondrocytes with H2O2. (spandidos-publications.com)
  • Our Guide-it in vitro transcription and screening kits for sgRNA include four kits for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies. (takarabio.com)
  • 632639) enables you to test the efficacy of different single guide RNAs (sgRNAs) in vitro prior to using them in studies involving Cas9-mediated gene editing so that you don't waste time delivering ineffective sgRNAs to your cells. (takarabio.com)
  • It has been previously shown that supplementing organotypic culture media with retinol improves the in vitro production of spermatids and spermatozoa in prepubertal mouse testicular tissues cultured at a gas-liquid interphase ( 5 , 7 ). (elifesciences.org)
  • By using both real-time reverse transcription-poly- voir to humans and the molecular basis of such a jump merase chain reaction TaqMan assay and an infectivity remain unanswered questions (12). (cdc.gov)
  • The volume of template used in the transcription reaction depends on the method of purification. (neb.com)
  • While all of the components in the HiScribe T7 In Vitro Transcription Kit are RNase-free, it is possible to introduce ribonucleases into the transcription reaction from the laboratory environment. (neb.com)
  • Panel A. 20 µl of each sgRNA in vitro transcription (IVT) reaction was incubated for 4 hr at 37°C. The yield for each transcribed sgRNA was quantified using a NanoDrop spectrophotometer. (takarabio.com)
  • This article provides an in-depth analysis of the reaction conditions for in vitro transcription (IVT) of full-length influenza H5 RNA, which is needed for diagnostic RNA production, for the T7 and SP6 phage promoter systems. (elsevierpure.com)
  • The kit contains all the reagents needed to in vitro transcribe and purify sgRNAs, and test their efficacy against a PCR target fragment using recombinant Cas9 nuclease. (takarabio.com)
  • Transcription Adaptation during In Vitro Adipogenesis and Osteogenesis of Porcine Mesenchymal Stem Cells: Dynamics of Pathways, Biological Processes, Up-Stream Regulators, and Gene Networks. (oregonstate.edu)
  • In the present study we have used transcriptome analysis to unravel pathways and biological functions driving in vitro adipogenesis and osteogenesis in BMSC and ASC. (oregonstate.edu)
  • In addition to these transcription factors, several peptide hormones (allatotropins, allatostatins and short neuropeptide F) and neurotransmitters (the biogenic amines octopamine, dopamine and glutamate) are also involved in the regulation of CA activity 16 . (nature.com)
  • In this webinar, we show how prototyping CRISPR components can be accelerated by combining a cell-free transcription and translation (TXTL) system with an Echo Acoustic Liquid Handler (LH) from Beckman Coulter Life Sciences. (beckman.com)
  • Transcription yields increase linearly for the first 90 minutes (approximately) and reach maximum after 2-3 hours. (neb.com)
  • 632635) is used to produce high yields of sgRNAs via in vitro transcription. (takarabio.com)
  • In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
  • In vitro effects were examined in human HepG2 hepatocytes, and U937 cells following 6, 24, or 48 h of exposure to PM extract or DMSO (control). (cdc.gov)
  • We report here that this sequence is necessary and sufficient for accurate basal transcription. (princeton.edu)
  • We recently reported a method for direct detection of viral RNA from a highly pathogenic human H5 influenza strain (A/Hanoi/30408/2005(H5N1)), which necessarily was transcribed in vitro from non-viral sources. (elsevierpure.com)
  • In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. (freedomontario26.ca)
  • Structure-function relationship and regulatory activity on in vitro transcription and on cell growth and differentiation. (unicam.it)
  • The chromatin peptides show a regulatory activity on DNA in vitro transcription and on cell growth and differentiation. (unicam.it)
  • By sequencing we identified a type II RNA hairpin loop terminator, which forms in a transcription direction-dependent fashion. (elsevierpure.com)
  • Surprisingly, 3'-end formation of U1 snRNAs synthesized in vitro is solely dependent upon elements within the U1 coding sequence. (cshl.edu)
  • In addition, a k-mean cluster analysis in association with enrichment analysis, networks reconstruction, and transcription factors overlapping analysis were performed in order to uncover the coordination of biological functions underlining differentiations. (oregonstate.edu)
  • Use the in-vitro gRNA transcription CRISPR kit to enzymatically synthesize gRNA targeting your sequence of interest. (agilent.com)
  • The H5 sequence was cloned with T7 and SP6 RNA polymerase promoters to allow transcription in either direction with either polymerase. (elsevierpure.com)
  • The T7 transcription product from purified, restricted plasmids in the vRNA direction only produced the 1,776-base full-length sequence and the 784-base fragment, instead of the three bands generated by the SP6 system, suggesting an early termination mechanism. (elsevierpure.com)
  • Optimized conditions are presented for the T7 and SP6 phage polymerase systems to minimize these early termination events during in vitro transcription of H5 influenza vRNA. (elsevierpure.com)
  • A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. (wikidata.org)
  • Further, partially purified YY1 can restore basal level transcription from a P5 + 1 element in a HeLa extract depleted for YY1 or a Drosophila embryo extract devoid of YY1 activity, whereas a YYl-specific antibody can block the reactivation. (princeton.edu)
  • The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
  • Bacterial allosteric transcription factors (aTFs) have been utilized as a novel class of recognition elements for in vitro biosensing, which circumvents the limitations of aTF-based whole-cell biosensors (WCBs) and helps to meet the increasing requirement of small-molecule biosensors for diverse applications. (firelight-fusion.com)
  • [ 5 ] The last decade has seen the cloning of the key elements of NER, and the process has been reconstituted in vitro. (medscape.com)
  • A complete in vitro spermatogenesis has been obtained from mouse prepubertal testicular tissue, although with low efficiency. (elifesciences.org)
  • In addition, viable and fertile mouse offspring have been obtained from in vitro produced spermatozoa by oocyte microinjection ( 3 , 4 ). (elifesciences.org)
  • A decline in intratesticular testosterone levels has been highlighted in vitro in 35- to 49-day cultures of 5 d pp mouse testes, i.e. after the end of the first wave of spermatogenesis ( 9 ). (elifesciences.org)
  • Transcription of a nematode U1 small nuclear RNA in vitro. (cshl.edu)
  • Cryopreserved prepubertal testicular biopsies could theoretically be later matured in vitro to produce spermatozoa for assisted reproductive technology. (elifesciences.org)