• Activation-induced deaminase (AID) can drive lymphomagenesis by generating off-target DNA breaks at loci that harbor highly active enhancers and display convergent transcription. (elifesciences.org)
  • Evidence from various studies has shown that genetic susceptibility to T2DM is polygenic [ 5 , 6 ], and Genome Wide Association Studies (GWAS) have reported more than 20 genetic loci associated with the risk of T2DM [ 7 , 8 ]. (hindawi.com)
  • Complex haploinsufficiency, which occurs when a strain containing two heterozygous mutations at distinct loci shows a phenotype that is distinct from either of the corresponding single heterozygous mutants, is an expedient approach to genetic interactions analysis in diploid organisms. (g3journal.org)
  • Typical approaches to GWAS exploit linkage disequilibrium (LD) between genetic variants such as SNPs and loci that directly affect traits of interest. (biomedcentral.com)
  • The collection and dissemination of genome-wide mRNA expression profiles among Add Health respondents make possible for the first time cutting-edge, population-based gene expression studies that map connections among social experiences, the genetic regulation of the immune system, biomarkers, and very common forms of inflammatory morbidity and morbidity-specific mortality. (unc.edu)
  • Overall, these studies identified genetic regulators of molecular diversity among individuals and provide new insights into mechanisms of gene regulation. (nature.com)
  • Because of the largely diploid nature of the human fungal pathogen Candida albicans , genetic interaction analysis has been limited to a small number of large-scale screens and a handful for gene-by-gene studies. (g3journal.org)
  • Here, we describe the construction of a barcoded-library of 133 heterozygous TF deletion mutants and deletion cassettes for designed to facilitate complex haploinsufficiency-based genetic interaction studies of the TF networks in C. albicans . (g3journal.org)
  • The genetic interaction networks at each stage of biofilm formation are significantly different indicating that the network is not static but dynamic. (g3journal.org)
  • One area of C. albicans genetic analysis that has not been extensively developed compared to other systems is genetic interaction analysis. (g3journal.org)
  • The first successful example of TF-based genetic engineering for increased NUE is the improvement of Arabidopsis growth under nitrogen-limited conditions, which was achieved using the maize TF gene Dof1 . (springer.com)
  • In this chapter, we summarize the concept underlying TF-based genetic engineering of NUE and provide a list of TFs shown to be potentially useful for genetically modifying NUE in the model plant Arabidopsis and several crop species. (springer.com)
  • Cell-free transcription-translation (TX-TL) systems offer a simple and fast alternative to performing these characterizations in cells. (caltech.edu)
  • Transcription, Genetic" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Although a DNA fragment encompassing the sarA transcript plus a 189-bp upstream region was sufficient for agr expression, complementation analysis revealed that the sarB transcript was the most effective in augmenting agr transcription as determined by RNAII and RNAIII transcription and gel retardation assays with the P2 and P3 promoters of agr. (asm.org)
  • To delineate the sar genetic determinant required for agr transcription, single copies of fragments from the sar locus, encompassing the individual sar transcripts (sarA, sarC, and sarB), were introduced into a sar mutant via the integration vector pCL84. (asm.org)
  • We also provide guidelines for designing TX-TL experiments to characterize new genetic networks. (caltech.edu)
  • However, a number of TFs are potentially useful for this type of genetic engineering. (springer.com)
  • In precursor leukemias, the most common genetic changes involve deleting, adding or rearranging segments of the DNA sequence. (elifesciences.org)
  • In the 1990's, he organized and led the team that deciphered the first genome sequence and genetic code for a salt-loving microbe, Halobacterium sp. (wikipedia.org)
  • Although TF-based genetic engineering is a promising approach for generating crops suitable for cropping systems requiring reduced fertilizer input, few such attempts have thus far been reported. (springer.com)
  • We have previously shown that the sar locus affects hemolysin production because it is required for agr transcription. (asm.org)
  • If cells are irradiated shortly after induction, the transcription of the DNA ceases, and the enzyme produced by the messenger RNA is observed to reach a maximum. (sciencemag.org)
  • Finally, we characterized the genetic interactions of a set of biofilm related TFs in the first two steps of biofilm formation, adherence and filamentation of adherent cells. (g3journal.org)
  • These transcribed medical transcription sample reports and examples are provided by various users and are for reference purpose only. (mtsamples.com)