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  • receptor
  • Our purpose herein was twofold: first, to identify some of the molecular determinants involved in TFII-I activation downstream of receptor crosslinking in T cells and second, to uncover the existence of Itk-TFII-I signaling in T lymphocytes. (umassmed.edu)
  • We report for the first time that TFII-I is tyrosine phosphorylated upon TCR, TCR/CD43, and TCR/CD28 co-receptor engagement in human and/or murine T cells. (umassmed.edu)
  • function
  • Our results provide an initial step in understanding the biological role of Itk-TFII-I signaling in T-cell function. (umassmed.edu)
  • important
  • We demonstrate that TFII-I is phosphorylated upon co-expression of WT, but not kinase-dead, or kinase-dead/R29C mutant Itk, suggesting these residues are important for TFII-I phosphorylation, presumably via an Itk-dependent mechanism. (umassmed.edu)
  • mRNA
  • By quantitative PCR we demonstrated a positive correlation of Coup-TFII and Ucp3 mRNA expression in skeletal muscle and brown adipose tissue in response to food deprivation and cold exposure, respectively. (nih.gov)
  • Coup-TFII mRNA is expressed in all examined tissue types as measured by qPCR. (nih.gov)
  • Ucp3 mRNA is found together with Coup-TFII mRNA in BAT and SKM. (nih.gov)
  • During transcription, a region of DNA is copied into mRNA. (psu.edu)
  • One strand of the DNA is used as a template during transcription to synthesize the mRNA. (psu.edu)
  • In a process termed transcription , regions of the DNA molecule are copied into messenger RNA (mRNA) , which, in turn, is used in translation to direct the synthesis of proteins. (psu.edu)
  • In a eukaryotic cell, transcription occurs in the nucleus and the newly synthesized pre-mRNA is modified in a variety of ways (referred to as RNA processing) and exported from the nucleus, whereupon a ribosome assembles on the mature mRNA in the cytoplasm. (psu.edu)
  • In a eukaryotic cell, transcription occurs in the nucleus and mRNA is modified in a variety of ways before it is exported out of the nucleus to the cytoplasm for translation. (psu.edu)
  • In general, the transcription of eukaryotic genes is more complex than that of prokaryotic genes, requiring additional enzymes (some specifically to carry out mRNA modification) as well as mechanisms to deal with the greater complexity of the eukaryotic genome. (psu.edu)
  • Upstream
  • In this study we in silico analyzed the upstream region of Ucp3 of the Djungarian hamster Phodopus sungorus and identified several putative response elements for Coup-TFII. (nih.gov)
  • motifs
  • 2. Basic region/Helix-Loop-Helix/leucine Zipper (bHLHZip) DNA-binding and dimerization motifs found in different combinations in a variety of coiled-coil/fibrous transcription factors that include: basic/leucine zipper ( bZip ) Basic/Helix-Loop-Helix ( bHLH ), and basic-Helix-loop-Helix-Zipper ( bHLHZip ) proteins. (coursehero.com)
  • GTF2IRD2 N-terminal half contains a leucine zipper motif, followed by 2 helix-loop-helix motifs (I repeats) that share homology with the TFII-I family of transcription factors. (abcam.com)
  • It is inferred to be a transcription factor based on the presence of GTF2I-like repeats (containing helix-loop-helix motifs), also found in other proteins such as GTF2IRD1 and GTF2I. (abcam.com)
  • markers
  • We examined expression of three interneuron precursor transcription factors, alongside other markers, using immunohistochemistry on 8-12 post-conceptional weeks (PCW) human telencephalon sections. (ncl.ac.uk)
  • Nuclear Receptor
  • Peroxisome proliferator-activated receptors (PPAR) are ligand-activated transcription factors and are members of the nuclear receptor superfamily, which includes steroid hormone, thyroid hormone, vitamin D, and retinoic acid receptors and orphan receptors ( 18 - 20 ). (aacrjournals.org)
  • processes
  • Our findings extend the general principle of component sharing among divergent nuclear processes and implicate TLS deficiency as a possible contributing factor in Williams-Beuren syndrome. (scienceopen.com)
  • The orchestration of these processes for different cell types requires the tight regulation of both intrinsic and extrinsic factors. (frontiersin.org)
  • This occurs through two sequential processes: transcription and translation. (psu.edu)
  • complex
  • Ucp3 is expressed in brown adipose tissue and skeletal muscle and controlled by a transcription factor complex including PPARalpha, MyoD and the histone acetyltransferase p300. (nih.gov)
  • Etiologic factors that contribute to development of ovarian cancer are complex. (aacrjournals.org)
  • regions
  • Mutations within Itk's kinase, pleckstrin-homology, and proline-rich regions did not abolish TFII-I-Itk binding. (umassmed.edu)
  • domain
  • Helix-turn-helix (HTH) DNA-binding motif found in a variety of globular transcription factors including Homeodomain proteins (i.e. products of homeotic genes) that specify parts/segments of the body and in other HTH variant proteins (winged-helix proteins, Pou domain proteins, and Paired domain proteins). (coursehero.com)