AnatomyOrganismsChemicals and DrugsPhenomena and ProcessesInformation Science
Transcription Factor TFIIIBRNA Polymerase IIITATA-Box Binding ProteinTranscription, GeneticTranscription FactorsTranscription Factors, TFIIITATA-Binding Protein Associated FactorsTATA BoxDNA-Binding ProteinsPromoter Regions, GeneticSaccharomyces cerevisiaeMolecular Sequence DataBase SequenceSp1 Transcription FactorGene Expression RegulationRNA, TransferSaccharomyces cerevisiae ProteinsBinding SitesProtein BindingTranscriptional ActivationTrans-ActivatorsNuclear ProteinsTranscription Factor TFIIBBasic Helix-Loop-Helix Transcription FactorsRNA, MessengerTranscription Factor AP-1Amino Acid SequenceCell LineRepressor ProteinsForkhead Transcription FactorsDNAHomeodomain ProteinsGene Expression Regulation, DevelopmentalMutationSignal TransductionBasic-Leucine Zipper Transcription FactorsCell NucleusTranscription Factor AP-2HeLa Cells
IIIBSubunitBRF1Polymerase III transcription repressionComplexesTFIIDTFIIIB150YeastTFIIICRepressionGenesFunctionalComplexProteinsInitiation factorsInteractsEukaryoticRNAsNegative regulatorCharacterizationActivityRegulatoryProteinMutationsMechanismsSynthesisChromatinRegulationMappingDomainsCommonFunction
IIIB1
  • High levels of RNA polymerase III gene transcription are achieved by facilitated recycling of the polymerase on transcription factor IIIB (TFIIIB)-DNA complexes that are stable through multiple rounds of initiation. (elsevierpure.com)
Subunit3
  • TFIIIB150 is a subunit of the TFIIIB transcription initiation complex, which recruits RNA polymerase III to target promoters in order to initiate transcription. (wikipedia.org)
  • The RPB9 subunit of RNA polymerase II regulates transcription elongation activity and is required for the action of the transcription elongation factor, TFIIS. (embl.de)
  • We describe the cognate cDNA cloning and characterization of two subunits (hTFIIIC63 and hTFIIIC102) that are present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC and are related in structure and function to two yeast TFIIIC subunits (yTFIIIC95 and yTFIIIC131) previously shown to interact, respectively, with the promoter (A box) and with a subunit of yeast TFIIIB. (embl.de)
BRF11
  • Furthermore, other rare diseases are also associated with mutations in genes encoding subunits of Pol III (POLR3H, POLR3GL) and the BRF1 component of the TFIIIB transcription initiation factor. (nih.gov)
Polymerase III transcription repression1
Complexes9
  • TFIIIB-DNA complexes in yeast comprise the TATA-binding protein (TBP), the TFIIB-related factor TFIIIB70, and TFIIIB90. (elsevierpure.com)
  • The high stability of the TFIIIB-DNA complex is conferred by TFIIIB90 binding to TFIIIB70-TBP-DNA complexes. (elsevierpure.com)
  • Here we present biochemical evidence that the high stability of TFIIIB-DNA complexes results from kinetic trapping of the DNA. (elsevierpure.com)
  • Thermodynamic analysis shows that the free energies of formation of TFIIIB70-TBP-DNA (ΔG° = -12.10 ± 0.12 kcal/mol) and TFIIIB-DNA (ΔG° = -11.90 ± 0.14 kcal/mol) complexes are equivalent whereas a kinetic analysis shows that the half-lives of these complexes (46 ± 3 min and 95 ± 6 min, respectively) differ significantly. (elsevierpure.com)
  • 2917366 . RA Buratowski S., Hahn S., Guarente L., Sharp P. A. RT Five Intermediate Complexes in Transcription Initiation by RNA Polymerase II RL Cell 56:549-561 (1989). (genexplain.com)
  • We have previously solved the structures of domains II and III, which stimulate arrested polymerase II elongation complexes in order to resume transcription. (embl.de)
  • Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that mediates transcription of class III genes through direct recognition of promoters (for tRNA and virus-associated RNA genes) or promoter-TFIIIA complexes (for the 5S RNA gene) and subsequent recruitment of TFIIIB and RNA polymerase III. (embl.de)
  • The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
  • Similarly, the transcription of small structural RNA genes (5S RNA, tRNA) by RNA polymerase III involves the assembly of common factors (TFIIIC and TFIIIB) into highly stable complexes via an ordered pathway. (rockefeller.edu)
TFIID1
TFIIIB1501
  • Transcription factor TFIIIB component B″ homolog also known as TFIIIB150 is a protein that in humans is encoded by the BDP1 gene. (wikipedia.org)
Yeast3
  • Domain I is conserved in evolution from yeast to human species and is homologous to the transcription factors elongin A and CRSP70. (embl.de)
  • Domain III has previously been shown to be readily exchanged between mammalian and yeast factors. (embl.de)
  • The only essential histone acetyltransferase (HAT) in yeast, Esa1, is part of the 1-MDa NuA4 complex, which plays pivotal roles in both transcription and DNA-damage repair. (nature.com)
TFIIIC1
  • Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III. (embl.de)
Repression3
  • Our broad objectives are to understand the specific regulatory events that control these processes, as well as more fundamental aspects of transcription activation and repression mechanisms. (rockefeller.edu)
  • All multicellular life relies on differential gene expression, determined by regulatory DNA elements and DNA-binding transcription factors that mediate activation and repression via cofactor recruitment. (sdbonline.org)
  • Screening more than 200,000 fragments covering the coding sequences of all transcription-related proteins in Drosophila melanogaster, this study identified 195 RDs in known repressors and in proteins not previously associated with repression. (sdbonline.org)
Genes2
  • The processes of animal cell growth, differentiation and infection by viruses result from the differential expression of specific genes, controlled primarily at the level of transcription. (rockefeller.edu)
  • To this end, our specific objectives are to determine the nature and mechanism of action of both the general transcription initiation factors that are commonly used by all genes and the gene- and cell type-specific factors that directly regulate target genes in response to various growth, developmental and viral stimuli. (rockefeller.edu)
Functional2
  • Most of the general factors (classes II and III) have been purified and individual subunits cloned for further structural and functional studies. (rockefeller.edu)
  • Our laboratory studies gene transcription by RNA polymerase (Pol) III and the functional impact of this system on normal and disease processes. (einsteinmed.edu)
Complex1
  • NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p. (nature.com)
Proteins3
  • Many proteins involved in eukaryotic transcription are similar in function and in sequence between organisms. (embl.de)
  • proteins that bind specifically to single-stranded G-rich DNA, proteins that bind to double-stranded DNA and proteins that interact with telomeric factors. (biomedcentral.com)
  • Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
Initiation factors1
  • DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
Interacts1
  • Domain I also interacts with the transcriptionally active RNA polymerase II holoenzyme and therefore, may have a function unrelated to the previously described transcription elongation activity of TFIIS. (embl.de)
Eukaryotic1
  • The products of Pol III transcription are small non-coding RNAs that have diverse and expanding functions in eukaryotic cells. (einsteinmed.edu)
RNAs1
  • Because of the functions of these RNAs, Pol III transcription is best known for its essential contribution to RNA maturation and translation. (nih.gov)
Negative regulator1
  • For these and other reasons, much of our research has focused on the Maf1 protein, a master negative regulator of Pol III transcription, and its posttranslational control by nutrient- and stress-signaling pathways. (einsteinmed.edu)
Characterization1
Activity1
  • Mutations in the N-terminal zinc ribbon had little effect on transcription activity. (embl.de)
Regulatory1
  • Some of the general factors have been shown to be direct targets for specific regulatory factors, while more recent studies have implicated a number of general and gene-specific cofactors in the function of gene-specific activators. (rockefeller.edu)
Protein3
  • The encoded protein localizes to concentrated aggregates in the nucleus, and is required for transcription from all three types of polymerase III promoters. (wikipedia.org)
  • For example, transcript elongation factor TFIIS is highly conserved among eukaryotes, and yet the TFIIS protein from Saccharomyces cerevisiae cannot function with mammalian RNA polymerase II and vice versa. (embl.de)
  • Differentially expressed miRNAs target 49 transcripts and their gene ontology enrichment analysis showed that the susceptible cultivar responds by modulating the processes of protein localization and pigment synthesis, whereas the resistant cultivar responds by modulating transcription factors and hormone signalling. (researchsquare.com)
Mutations1
  • By contrast, mutations in the acidic loop that connects the second and third beta-strands of the C-terminal zinc ribbon were completely inactive for transcription. (embl.de)
Mechanisms1
  • Our studies are also directed toward the mechanisms by which these factors in turn are regulated in the various biological responses. (rockefeller.edu)
Synthesis1
  • Our current mechanistic understanding of these phenotypes is based on a novel futile RNA cycle hypothesis wherein deregulated Pol III transcription serves as an energy sink, consuming energetically costly nucleotides in the wasteful synthesis of RNA that does not accumulate and is mostly degraded. (einsteinmed.edu)
Chromatin1
  • It is phosphorylated by casein kinase 2 during mitosis, resulting in its release from chromatin and suppression of polymerase III transcription. (wikipedia.org)
Regulation1
  • Proper regulation of Pol III transcription is critical for balanced growth and its deregulation is a key event in cell transformation and tumorigenesis. (einsteinmed.edu)
Mapping1
  • Mapping of domains required for transcription elongation. (embl.de)
Domains2
  • TFIIS is a transcription elongation factor that consists of three domains. (embl.de)
  • The individual zinc ribbon domains, in isolation or in combination, could not stimulate transcription by a polymerase lacking RPB9, pol IIDelta9. (embl.de)
Common1
Function2
  • To elucidate the relationship between the structure and transcription elongation function of RPB9, we initiated a mutagenesis study on the Saccharomyces cerevisiae homologue. (embl.de)
  • Despite the sequence similarities, there are many factors that do not function across species. (embl.de)