• We have identified mutations affecting components of three different complexes involved in the control of basal transcription: the mediator, TFIIH, and RNA pol II itself. (genetics.org)
  • We show that mutations in the putative phosphorylation sites of TFIIAβ greatly affect degradation of the β‐subunit. (embopress.org)
  • The regulation of C/EBPβ is exerted in many manners, phosphorylation , acetylation , activation and repression via others transcription factors, oncogenic elements or chemokines , autoregulation, etc. (bionity.com)
  • However, the precise function and general requirement for a TFIIA-TBP association in vivo have not been completely elucidated. (asm.org)
  • Triplex DNA can disrupt gene transcriptions and can be used as of this oncogene in vivo. (oalib.com)
  • Our results support the existence of different components within the basic transcription complexes that antagonistically interact with the NC2 repressor and suggest that the correct balance between the activities of specific positive and negative components is essential for cell growth. (genetics.org)
  • Interaction of TFIIA with TBP also results in the exclusion of negative (repressive) factors that might otherwise bind to TBP and interfere with PIC formation. (wikipedia.org)
  • TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. (cellsignal.com)
  • However, subsequent work has shown that the ADs of transactivators can also directly bind chromatin-directed coactivators to modulate transcription ( 1 ). (pubmedcentralcanada.ca)
  • Examples of this correlation have been demonstrated for hyperacetylated H4 in chromatin near the active chicken globin gene ( 3 ), the human platelet-derived growth factor gene ( 4 ), and the Drosophila male X chromosome ( 5 ). (pnas.org)
  • Genetic tests reveal that cmi is required for proper global trimethylation of H3K4 and that hormone-stimulated transcription requires chromatin binding by CMI, methylation of H3K4 by TRR and demethylation of H3K27 by the demethylase UTX. (biologists.org)
  • These factors recruit a series of chromatin co-activators that can covalently modify nucleosomes at specific histone residues (not identified) and mobilize nucleosomes through ATP-requiring reactions. (nih.gov)
  • Transcription is initiated at regions of DNA called promoters, which are typically 20 to 150 base pairs long, depending on the organism. (encyclopedia.com)
  • Co-factors can be activators or repressors and are typically recruited to gene promoters through associations with receptors ( Bulynko and O'Malley, 2011 ). (biologists.org)
  • This gene, ALF/TFIIAtau (gene name GTF2A1LF) is expressed only in oocytes and spermatocytes, suggesting it has a TFIIA-like regulatory role for gene expression only in germ cells. (wikipedia.org)
  • Classical studies have shown that ADs enhance transcription rates by directly stimulating the formation and/or function of the PIC, which is an assortment of over 40 distinct polypeptides often termed the general transcription factors TFIIA, -B, -D, -E, -F, -H, and RNA pol II ( 2 , 3 ). (pubmedcentralcanada.ca)
  • Nuclear receptors (NRs) function as transcription factors that respond to cellular signals to initiate new gene expression programs ( King-Jones and Thummel, 2005 ) and have essential roles in embryonic development, growth and differentiation. (biologists.org)
  • Mutations in the CRS that prevent cleavage significantly prolong the half‐life of TFIIA. (embopress.org)
  • In a previous report, a genetic approach was used to determine that glucose dehydrogenase activity (GDH) is required for inhibiting prodigiosin production and transcription of the prodigiosin biosynthetic operon ( pigA-N ). However, the transcription factor(s) that regulate this process were not characterized. (oalib.com)
  • This observation and data presented in this study suggest that cleavage of TFIIAαβ precursor is a regulated process and that the cleaved (α+β+γ) and uncleaved (αβ+γ) TFIIA may have distinct cellular functions. (embopress.org)