• Furthermore, we show that in maintaining early adipose precursors, Sox9 activates Meis1, which prevents adipogenic differentiation. (nih.gov)
  • It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene. (wikipedia.org)
  • SOX9 has a role in sex determination and differentiation of Sertoli cells. (thermofisher.com)
  • The high-mobility group (HMG) domain is a DNA-binding motif that is shared abundant non-histone components of chromatin and by specific regulators of transcription and cell differentiation. (embl.de)
  • The SRY-box 4 (SOX4) gene, which is located on chromosome 6p22.3, encodes a 47-kDa protein that is a member of the sex-determining region Y-related high-mobility group-box transcription factor family and has functions in embryonic development and cell differentiation ( 3 , 4 ). (spandidos-publications.com)
  • The Sox9 gene is required for the differentiation of mesenchymal precursor cells to chondrocytes and, together with the related L-Sox5 and Sox6 proteins, controls chondrocyte-specific gene expression [ 4 , 5 ]. (biomedcentral.com)
  • These repressors could down-regulate CRE-dependent transcription and thus cause delay of cell cycle progression and/or promote cell cycle exit during terminal differentiation. (biomedcentral.com)
  • It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. (sitoolsbiotech.com)
  • these cells are able to secrete soluble factors that stimulate the migration, mitosis, and differentiation of local stem cells. (biomedcentral.com)
  • Both transcription factor-based reprogramming and directed differentiation approaches ultimately exploit transcription factors to influence cellular identity. (silverchair.com)
  • In vivo and in vitro experiments confirmed that the knockdown of Sox9 improved the inflammatory response and fibrosis of mouse kidney tissues and HK-2 cells, while the ameliorative effect of silencing Sox9 was inhibited by overexpression of IL-6. (figshare.com)
  • Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
  • In addition, overexpression of SOX9 activated the mTOR pathway partly through BMP2. (cancerindex.org)
  • We therefore hypothesized that SOX9 overexpression may be responsible for the neoplastic-like phenotype observed in our model. (cdc.gov)
  • Here we demonstrate that overexpression of ATF3 in primary mouse chondrocytes results in reduced transcription of both genes, as well as decreased activity of a CRE reporter plasmid. (biomedcentral.com)
  • In parallel, ATF3 overexpression reduces the activity of a SOX9-dependent promoter and increases the activity of a RUNX2-dependent promoter. (biomedcentral.com)
  • The overexpression of a single transcription factor in MSCs may promote transdifferentiation into a specific cell lineage, which could then be used for the treatment of some diseases. (biomedcentral.com)
  • On the other hand, male sex determination is triggered by sex-determining region Y (SRY), which is located on the Y chromosome and works as a master regulator, initiating SOX9 expression. (benthamscience.com)
  • SOX9 expression was found to colocalize with a proportion of so-called scattered tubular cells in the uninjured kidney, a cell population previously shown to be involved in kidney injury and regeneration. (lu.se)
  • Sox9 was significantly enriched in the promoter region of miR-96-5p and repressed miR-96-5p expression. (figshare.com)
  • Expression of SIRT1/SOX9/LCN2 in the hypoxic cells was determined, and their interactions were predicted by bioinformatics websites and followed by the verification by luciferase assay and chromatin immunoprecipitation (ChIP). (hindawi.com)
  • To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
  • Expression of SOX9 did not decrease during aging and was instead upregulated by reactive astrocytes in a number of settings, including a murine model of amyotrophic lateral sclerosis (SOD1G93A), middle cerebral artery occlusion, and multiple mini-strokes. (jneurosci.org)
  • It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. (thermofisher.com)
  • Most of the enhancers activated by EGFRvIII bore signature DNA sequences that are bound by dozens of transcription factors--regulators of gene expression--expressed at high levels in GBM. (sciencedaily.com)
  • The researchers show that as EGFRvIII alters the epigenetic landscape of activated enhancers, it also noticeably boosts the expression of SOX9 and FOXG1. (sciencedaily.com)
  • To find out if this potentially therapeutic effect might be induced by a drug, the researchers examined the genes whose expression is controlled by SOX9 and FOXG1. (sciencedaily.com)
  • This protein controls the expression and activity of yet another transcription factor, cMyc, which plays a central role in driving the aberrant metabolism and growth of GBM cells. (sciencedaily.com)
  • Mechanistically, SOX9 bound directly to the promoter region of BMP2 and increased BMP2 expression. (cancerindex.org)
  • The researchers showed that human pluripotent stem cell (hPSC)-derived neural stem cells engineered with reduced expression of a gene called SOX9 differentiate preferentially into spinal motor neurons. (genengnews.com)
  • Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
  • In the developing male (XY) gonad, sex-determining region of the Y (SRY) protein acts to up-regulate expression of the related gene, SOX9 , a transcriptional regulator that in turn initiates a downstream pathway of testis development, whilst also suppressing ovary development. (plos.org)
  • Expression analysis of mutant XY gonads at the same stage also reveals a dramatic reduction in Sox9 and, crucially, Sry at the transcript and protein levels. (plos.org)
  • The Y-linked sex determining gene SRY regulates this process by initiating a pathway of gene and protein expression, including the expression of critical autosomal genes such as SOX9 . (plos.org)
  • Our data suggest that transcriptional induction of the Atf3 gene in maturing chondrocytes results in down-regulation of cyclin D1 and cyclin A expression as well as activation of RUNX2-dependent transcription. (biomedcentral.com)
  • Protein expression was investigated by ELISA and immunofluorescence, transcription factor-DNA binding by ELISA, gene expression by real-time PCR, prostaglandin E 2 (PGE 2 ) by radioimmunoassay, and matrix metalloproteinase (MMP) activity and nitric oxide (NO) production by fluorometry. (karger.com)
  • To identify novel genes regulated by SOX9 we investigated changes in gene expression by microarray analysis following retroviral transduction with SOX9 of a human chondrocytic cell line (SW1353). (liverpool.ac.uk)
  • Second, during dedifferentiation of HACs by passage in monolayer cell culture, during which the expression of SOX9 progressively decreased, four of the genes (S100A1, RGC32, CRTL1 and SPINT1) also decreased in their expression. (liverpool.ac.uk)
  • Third, in samples of osteoarthritic (OA) cartilage, which had decreased SOX9 expression compared with age-matched controls, there was decreased expression of SRPX, APOD, RGC32, CRTL1 and SPINT1. (liverpool.ac.uk)
  • The results showed that a group of genes identified as being upregulated by SOX9 in the initial SW1353 screen were also regulated in expression in healthy and OA cartilage. (liverpool.ac.uk)
  • Other genes initially identified were differently expressed in isolated OA chondrocytes and their expression was unrelated to changes in SOX9. (liverpool.ac.uk)
  • The results thus identified some genes whose expression appeared to be linked to SOX9 expression in isolated chondrocytes and were also altered during cartilage degeneration in osteoarthritis. (liverpool.ac.uk)
  • Ectopic expression of vector-delivered transcription factors (particularly the triumvirate of Pdx1, Ngn3 and MafA) induces reprogramming through broad transcriptional remodelling. (silverchair.com)
  • We examine how transdifferentiation protocols are evolving to ever more faithfully recapitulate normal developmental biology using increasingly sophisticated biomimetic techniques and ectopic transcription factor expression. (silverchair.com)
  • It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. (neobiotechnologies.com)
  • En-1 blocks Wnt-7a expression, preventing expression of Lmx-1b there, and establishes the dorsal-ventral axis. (medscape.com)
  • The following product was used in this experiment: SOX9 Monoclonal Antibody (1B11) from Thermo Fisher Scientific, catalog # MA5-17177, RRID AB_2538648. (thermofisher.com)
  • Formalin-fixed, paraffin-embedded human pancreas stained with SOX9 Recombinant Rabbit Monoclonal Antibody (SOX9/3916R). (neobiotechnologies.com)
  • SDS-PAGE Analysis Purified SOX9 Recombinant Rabbit Monoclonal Antibody (SOX9/3916R). (neobiotechnologies.com)
  • Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
  • Order a complex pool of 30 siRNAs (siPOOL) for the specific gene knockdown of human gene SOX9. (sitoolsbiotech.com)
  • Conclusion: Collectively, Sox9 up-regulates miR-96-5p-mediated Trib3 and activates the IL-6 signaling pathway to exacerbate the inflammatory response, ultimately promoting the development and progression of kidney injury. (figshare.com)
  • The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene. (wikipedia.org)
  • Next, Sox9 activates FGF9 and forms feedforward loops with FGF9 and PGD2. (wikipedia.org)
  • Transcription factor SOX-9 is a protein that in humans is encoded by the SOX9 gene. (wikipedia.org)
  • The Sox9 protein has been implicated in both initiation and progression of multiple solid tumors. (wikipedia.org)
  • One member of this family, Activating Tanscription Factor 3 (ATF3), is expressed during skeletogenesis and acts as a transcriptional repressor, but the function of this protein in chondrogenesis is unknown. (biomedcentral.com)
  • One example is the activating transcription factor/cyclicAMP response element-binding protein (ATF/CREB) family that is defined by the ability of its members to bind to the cyclicAMP response element (CRE) in target promoters. (biomedcentral.com)
  • In mechanistic studies, we found that exogenous supplementation of suramin can activate Nrf2, and accordingly inhibit the nuclear factor kappa-light-chain-enhancer of activated B cells (NF- κB) and mitogen-activated protein kinase (MAPK) pathways, thereby alleviating the inflammation and ECM degeneration of chondrocytes stimulated by IL-1ß. (bvsalud.org)
  • SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
  • Introduction: Our study investigated the possible mechanisms of the role of the transcription factor Sox9 in the development and progression of kidney injury through regulation of the miR-96-5p/Trib3/IL-6 axis. (figshare.com)
  • A previous study has shown the key roles of SOX9 in the progression of CNV [ 1 ]. (hindawi.com)
  • Therefore, it can be hypothesized that SIRT1/SOX9/LCN2 axis may exert great functions on the progression of CNV in AMD, but their specific interactions in CNV-induced AMD remain unclear. (hindawi.com)
  • SOX4 has been revealed to induce EMT, a key process during organ development and the progression of epithelial tumors to metastatic cancers ( 9 ), via its activation of the Wnt and transforming growth factor β signaling pathways in cancer cells ( 10 , 11 ). (spandidos-publications.com)
  • 2013. Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients prognosis. (neobiotechnologies.com)
  • Purified recombinant fragment of human SOX9 expressed in E. Coli. (thermofisher.com)
  • The MA5-17177 immunogen is purified recombinant fragment of human SOX9 expressed in E. Coli. (thermofisher.com)
  • Recombinant fragment within Human SOX9 aa 150-300. (abcam.com)
  • These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. (thermofisher.com)
  • Notably, silencing of the two transcription factors stopped tumor growth, both in cell cultures and in an animal model that mimics GBM. (sciencedaily.com)
  • We found that SOX9 is almost exclusively expressed by astrocytes in the adult brain except for ependymal cells and in the neurogenic regions, where SOX9 is also expressed by neural progenitor cells. (jneurosci.org)
  • We quantified the relative number of astrocytes using the isotropic fractionator technique in combination with SOX9 immunolabeling. (jneurosci.org)
  • Based on SOX9 immunolabeling, we document that astrocytes constitute a smaller fraction of total cell number than previously estimated in the normal adult mouse brain. (jneurosci.org)
  • Overall, SIRT1 promoted the formation of CNV-induced AMD through SOX9 deacetylation-caused LCN2 upregulation, representing a promising target for CNV-induced AMD management. (hindawi.com)
  • The identification of key master regulator transcription factors (which distinguish one body part from another) during embryonic development has been central in developing transdifferentiation protocols. (silverchair.com)
  • Specifically, Sox9 appears to induce invasiveness and therapy-resistance in prostate, colorectal, breast and other cancers, and therefore promotes lethal metastasis. (wikipedia.org)
  • We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
  • Acts early in neural crest formation, functioning redundantly with the other group E Sox factors sox8 and sox9 to induce neural crest progenitors. (xenbase.org)
  • Taken together, our findings provide a mechanistic insight into SWCNT-induced carcinogenesis and the role of SOX9 in CSC regulation and metastasis. (cdc.gov)
  • LCN2 was increased by SOX9 and SIRT1 deacetylated SOX9 to promote its nuclear translocation, which further inhibited the viability of human retinal pigment epithelial cells and promoted cell apoptosis and angiogenesis as well as CNV-induced AMD formation. (hindawi.com)
  • Additionally, SIRT1 can regulate the acetylation of sex determining region Y- (SRY-) box 9 (SOX9) through nuclear translocation [ 10 ]. (hindawi.com)
  • These findings shed light on the functional and regulatory aspects of SOX9 activation in the human kidney during injury and regeneration. (lu.se)
  • SOX9 belongs to the SOX gene family expressed in several organisms and involved in numerous physiological processes such as sex determination and development gonad [ 11 ]. (hindawi.com)
  • A team of Ludwig Cancer Research scientists has mapped out how a mutant version of the epidermal growth factor receptor (EGFR) known as EGFRvIII specifically drives critical processes that alter the reading of the genome to fuel the growth of the brain cancer glioblastoma multiforme and -- most important -- how each process is linked to the other. (sciencedaily.com)
  • HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
  • Transcription factors binding to the cyclicAMP response element (CRE) are known to regulate these processes. (biomedcentral.com)
  • These processes are regulated by a large number of endocrine, paracrine and autocrine hormones and growth factors that, to a large part, act on chondrocyte cell surface receptors. (biomedcentral.com)
  • SRY is a transcription factor (involved in turning on genes) whose proximal target is another transcription factor, SOX9. (allandyenshapiro.com)
  • Collectively, these results identify a novel role for SOX9 as a potential therapeutic marker for the prevention and treatment of NPC. (cancerindex.org)
  • Additionally, SOX9-positive cells demonstrate a capacity to differentiate toward other nephron segments. (lu.se)
  • Following injury and in areas adjacent to inflammatory cell infiltrates, SOX9-positive cells were increased in number. (lu.se)
  • Studies in chondrocyte cell line has revealed nearly 50% of SOX9 is bound to DNA and it is directly regulated by external factors. (wikipedia.org)
  • MA5-17177 targets SOX9 in indirect ELISA, ICC, IF and WB applications and shows reactivity with Human samples. (thermofisher.com)
  • SOX9 is mostly localised in the nucleus and it is highly mobile. (wikipedia.org)
  • We show the requirement of Sox9 for the maintenance of Pref-1 + proliferative, early precursors. (nih.gov)
  • In the interstitial tissue, Leydig cells synthesize androgens and the insulin-like factor 3 (INSL3) [ 3 ], whereas in the seminiferous tubules, Sertoli cells regulate the nutrients and factors that reach the germ cells by means of the blood-testis barrier. (biomedcentral.com)
  • Induction of SRY box transcription factor 9 (SOX9) has been shown to occur in response to kidney injury in rodents, where SOX9-positive cells proliferate and regenerate the proximal tubules of injured kidneys. (lu.se)
  • An in vivo animal model of kidney injury and an in vitro cellular model of kidney injury were constructed using LPS induction in 8-week-old female C57BL/6 mice and human normal renal tubular epithelial cells HK-2 for studying the possible roles of Sox9, miR-96-5p, Trib3, and IL-6 in kidney injury. (figshare.com)
  • Here, we used the Pref-1 promoter-rtTA system in mice for labeling Pref-1 + cells and for inducible inactivation of the Pref-1 target Sox9. (nih.gov)
  • Upon Sox9 inactivation, these Pref-1 + cells become PDGFRα + cells that express early adipogenic markers. (nih.gov)
  • Thus, we show that Pref-1 + cells precede PDGFRα + cells in the adipogenic pathway and that Sox9 inactivation is required for WAT growth and expansion. (nih.gov)
  • Highly expressed LCN2, SOX9, and SIRT1 were observed in hypoxic cells. (hindawi.com)
  • In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. (cancerindex.org)
  • The researchers reported on their developments in Advanced Science , in a paper titled, " Transplanting Human Neural Stem Cells with ≈50% Reduction of SOX9 Gene Dosage Promotes Tissue Repair and Functional Recovery from Severe Spinal Cord Injury ," in which they concluded, "Our findings represent a new paradigm in generating genetically modified hNSCs for the treatment of SCI. (genengnews.com)
  • In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
  • SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
  • BTE aims to combine the cellular ( osteogenic cells), extracellular ( osteoconductive scaffolds) and/or molecular elements ( osteoinductive growth factors) required for bone healing [ 2 ]. (biomedcentral.com)
  • ASC treatment upregulated granulocyte-macrophage colony stimulating factor (GM-CSF) at the gene level, which may contribute to the recruitment of cells that participate in skin repair to the site of injury. (biomedcentral.com)
  • Increasingly, complimentary cell culture techniques, which recapitulate the developmental microenvironment, are employed to coax cells to adopt new identities by indirectly regulating transcription factor activity via intracellular signalling pathways. (silverchair.com)
  • Here, we discuss how transcription factors, and their unique position as the gatekeepers of cellular identity, are exploited in cell reprogramming protocols by exploring work focusing on one reprogramming paradigm - the transdifferentiation of hepatocytes to pancreatic beta cells (β-cell). (silverchair.com)
  • a hostile microenvironment and deficiency of growth factors in the injured spinal cord limited the therapeutic effects of grafted NSCs that are largely determined by their survival, neurogenic potency, integration capacity, and axial identity," the researchers continued. (genengnews.com)
  • MA5-17177 detects SOX9 which has a predicted molecular weight of approximately 56kDa. (thermofisher.com)
  • During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. (thermofisher.com)
  • Here, we characterized the role of SOX9 in normal and injured human kidneys. (lu.se)
  • ASO Author Reflections: Role of SOX9 Transcription Factor in Pancreatic Neoplasms. (asbestos.com)
  • Accumulating evidence has shown that SOX9 plays a significant role in various malignancies. (cancerindex.org)
  • However, the role of SOX9 in nasopharyngeal carcinoma (NPC) remains unknown. (cancerindex.org)
  • SOX9 plays an important role in the normal skeletal development. (neobiotechnologies.com)
  • A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)
  • Hence, the present study was conducted to verify the hypothesis and explore the specific mechanism among SIRT1, SOX9, and LCN2 in the development of CNV-induced AMD, which will be greatly helpful to enlarge the understanding of CNV-induced AMD and to validate a novel therapy. (hindawi.com)
  • The latter causes urogenital development, a highly complex process, through a complex cascade of transcription factors and signaling events. (benthamscience.com)
  • Wnt-7a stimulates secretion of transcription factor Lmx1b, which causes dorsal development in this part of the limb bud. (medscape.com)
  • An enhancer (a control element) in the promoter of the SOX9 gene is duplicated. (allandyenshapiro.com)
  • Results: Sox9 was highly expressed in both mouse and cellular models of kidney injury. (figshare.com)