• In this study, we determined the effect of DMF on platelet-derived growth factor (PDGF)-BB- and TNFα-induced asthma-relevant cytokines and NF-κB activation by primary human asthmatic and nonasthmatic airway smooth muscle cells (ASMC). (edu.au)
  • Recently, TDP-43 was reported to contribute to pathogenesis in amyotrophic lateral sclerosis through its interaction with p65 nuclear factor κB (NF-κB) resulting in abnormal hyperactivation of this signaling pathway in motor neurons. (nih.gov)
  • In the classic NF-κB pathway, family members p65 (RelA) and p50 (NF-κB1) are localized in the cytoplasm of unstimulated cells as inactive dimers bound to inhibitors of NF-κB (IκB). (aacrjournals.org)
  • Furthermore, semi-quantitative RT-PCR, Western blot and a luciferase reporter assay were used to assess the effects of hERG1 inhibition on the nuclear factor-κB (NF-κB) pathway. (jcancer.org)
  • This correlated to a significant increase in tumor necrosis factor-α concentration in cell culture media.This work provides solid evidence that differentiated human muscle precursor cells maintain in vivo phenotypes of inflammation and insulin resistance and that obesity alone may not be sufficient to establish inflammation in these cells.It is important that we demonstrate an anti-inflammatory role for AMPK in these human cells. (nih.gov)
  • The level of inflammation (nuclear factor-κB [NF-κB] signaling) and effect of pharmacological AMPK activation was assessed by Western blots, enzyme-linked immunosorbent assay, and radioactive assays (n = 5 for each subject group). (nih.gov)
  • Despite attenuation of NF-κB activity by AMPK, insulin resistance in obese T2D cells remained, suggesting factors in addition to inflammation may contribute to the insulin resistance phenotype in muscle cells. (nih.gov)
  • Chronic gastric inflammation induced by the bacterial pathogen, H. pylori , is the strongest known risk factor for the development of atrophic gastritis, metaplasia, dysplasia, and ultimately gastric adenocarcinoma (Figure 1 ). (frontiersin.org)
  • NF-κB transcription factors control the expression of various genes regulating inflammation, cell survival, and proliferation. (aacrjournals.org)
  • In AML, cellular proliferation, differentiation, and apoptosis are affected by complementing genetic alterations that deregulate signaling and transcription pathways (reviewed in ref. 1 ). (aacrjournals.org)
  • Inactive NFKB is sequestered in the cytoplasm through binding to inhibitors of NFKB (IKBs) until activation by IKB kinases, which results in the release and ubiquitination of IKB and the subsequent translocation of NFKB dimers into the nucleus regulating transcription of responsive genes [ 1 , 2 ]. (pubmedcentralcanada.ca)
  • Recent studies have shown that c-Rel, a member of the NF-κB family of transcription factors, is essential for LPS-induced IL-12p40 production by macrophages. (jimmunol.org)
  • Many of the microbial products which stimulate the innate production of IL-12 from accessory cells such as macrophages and dendritic cells (DC) 3 signal through Toll-like receptors (TLR) which activate the NF-κB family of transcription factors ( 2 , 3 , 4 ). (jimmunol.org)
  • Chronic infection with Helicobacter pylori is the strongest known risk factor for the development of gastric cancer. (frontiersin.org)
  • The major contributing factor to the development of gastric cancer is colonization and chronic infection by the bacterial pathogen, Helicobacter pylori . (frontiersin.org)
  • 20 Importantly, the active form of NF-κB is known to have antiapoptotic activity and is thereby considered to be a key survival factor for several types of cancer. (bloodjournal.org)
  • Reverse transcription-PCR and Western blotting indicated that pancreatic cancer cells expressed only ADMR but not CRLR. (aacrjournals.org)
  • Whereas most drug-sensitive cell lines and patient biopsies showed high expression and activity of the melanocytic lineage transcription factor MITF, intrinsically resistant cell lines and biopsies displayed low MITF expression but higher levels of NF-κB signaling and the receptor tyrosine kinase AXL. (aacrjournals.org)
  • In addition to bacterial determinants, numerous host and environmental factors increase the risk of gastric carcinogenesis. (frontiersin.org)
  • Expression level of risk genes of MHC class II is a susceptibility factor for autoimmunity: New insights. (amedeo.com)
  • Taken together, these data indicate that primitive AML cells aberrantly express NF-κB and that the presence of this factor may provide unique opportunities to preferentially ablate LSCs. (bloodjournal.org)
  • Expression of BCR-ABL allows myeloid cells to grow in the absence of the growth factors interleukin-3 and granulocyte-macrophage colony-stimulating factor. (aacrjournals.org)
  • Transcription factor ELISA demonstrated an overall increase in RELA during the peri-implantation period in both cyclic and pregnant gilts. (pubmedcentralcanada.ca)
  • The pregnancy-specific increase of IL1B in the uterine lumen during conceptus elongation and attachment in pigs [ 3 ] may contribute to the activation of NFKB and the transcription of regulated genes, such as prostaglandin synthase-2 (PTGS2). (pubmedcentralcanada.ca)
  • Naive CD4 T cell activation in the presence of IL-12 leads to the expression of the master transcription factor T-bet that induces IFN-γ production and thus polarization toward the Th1 cell fate ( 6 ). (jimmunol.org)
  • In contrast, Th17 cell differentiation relies on IL-6, in cooperation with TGF-β and IL-1β, to trigger the expression of the master transcription factor RORγt (encoded by Rorc ) that governs IL-17-expression, whereas maintenance is controlled by IL-23 ( 13 ). (jimmunol.org)
  • The objective of the current investigation was to determine changes in expression and cellular localization of NFKB and associated factors during the estrous cycle and early pregnancy in the pig. (pubmedcentralcanada.ca)