• Hey basic helix-loop-helix transcription factors are repressors of GATA4 and GATA6 and restrict expression of the GATA target gene ANF in fetal hearts. (jax.org)
  • The lack of suppression of PDX1, PTF1A, GATA4, and GATA6 indicates that MODY5-mediated pancreatic hypoplasia is mechanistically independent. (harvard.edu)
  • Bennett J, Baumgarten SC, Stocco C. GATA4 and GATA6 Silencing in Ovarian Granulosa Cells Affects Levels of mRNAs Involved in Steroidogenesis, Extracellular Structure Organization, IGF-I Activity, and Apoptosis. (chicagobiomedicalconsortium.org)
  • Mutations in the Gata4 gene cause human congenital cardiomyopathies, including valve and septal defects and loss of both Gata4 and Gata6 in mice leads to acardia, suggesting that genetic interactions between these factors are essential for the onset and/or maintenance of cardiogenesis. (xenbase.org)
  • Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis. (musc.edu)
  • GATA transcription factors direct cell proliferation and differentiation in many organs, and are implicated in tumorigenesis. (tuni.fi)
  • We studied in detail the expression patterns of these two GATA factors and a GATA-6 down-stream target, Indian hedgehog (Ihh), in normal human gastrointestinal mucosa. (tuni.fi)
  • The expression of the carcinogenesis-related protein Indian hedgehog was also investigated in comparison to GATA factors. (tuni.fi)
  • Transcription factor GATA-6, also known as GATA-binding factor 6 (GATA6), is protein that in humans is encoded by the GATA6 gene. (wikipedia.org)
  • GATA-6, a zinc finger transcription factor, is important in the endodermal differentiation of organ tissues. (wikipedia.org)
  • Master regulatory GATA transcription factors: mechanistic principles and emerging links to hematologic malignancies. (rochester.edu)
  • In an early step, the Dorsocross genes act in parallel with tinman to activate the expression of pannier , a cardiogenic gene encoding a Gata factor. (biologists.com)
  • In all these organisms, cardiac induction in the lateral mesoderm involves the concerted action of signals mediated by Bmp, Wnt and Fgf family members, which act upon cardiogenic genes encoding NK homeodomain, Gata and T-box transcription factors (reviewed by Zaffran and Frasch, 2002 ). (biologists.com)
  • W e recently discovered an epigenetic switch in GATA isoform expression that results in the replacement of GATA2 in normal healthy endometrium with GATA6 in endometriotic cells. (northwestern.edu)
  • We posit that GATA isoform expression affects critical cell fate decisions in the steroid - hormone sensitive stromal cells of the endometrium, and that the targets of GATA transcription factors in these cells contribute to the pathogenesis of endometriosis. (northwestern.edu)
  • TCF7L2 (Transcription Factor 7-Like 2) Regulation of GATA6 (GATA-Binding Protein 6)-Dependent and -Independent Vascular Smooth Muscle Cell Plasticity and Intimal Hyperplasia. (bvsalud.org)
  • In addition, we demonstrate that this family is critical in maintaining stemness of EpCAM + AFP + HepCSCs, in part by directly targeting an inhibitor of Wnt/β-catenin signaling (nemo-like kinase [NLK]) and two hepatic transcriptional regulators of differentiation, i.e., caudal type homeobox transcription factor 2 (CDX2) and GATA binding protein 6 (GATA6). (biomedcentral.com)
  • Congenital Heart Disease (CHD) is the leading cause of death in the first year of life and recent discoveries have identified GATA family of transcription factors and Wnt signalling molecules as key conserved regulators involved in early cardiogenesis. (xenbase.org)
  • Of the six vertebrate members of the GATA factors three (GATA4, 5 and 6) are expressed in the heart in partially overlapping patterns to carry out partially redundant functions. (xenbase.org)
  • I use Xenopus as a model organism to examine the fundamental question about what functional roles individual GATA factors play during cardiogenesis. (xenbase.org)
  • Pancreatic agenesis has been associated with pathogenic variants in the PDX1 gene, which encodes the insulin promoter factor-1 (IPF-1) transcription factor, and PTF1A gene which is associated with an autosomal recessive syndrome of neonatal diabetes mellitus with cerebellar and/or pancreatic agenesis. (uchicago.edu)
  • Recently, heterozygous pathogenic variants in GATA6 are considered as the most common cause of pancreatic agenesis. (uchicago.edu)
  • Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
  • Furthermore, these hUC-MSCs express a set of hepatic transcription factors (GATA4, GATA6, SOX9 and SOX17) and other hepatic markers (DKK1, DPP4, DSG2, CX43, KRT18 and KRT19), rendering them an interesting stem cell population for the development of human hepatocyte-like cells. (re-place.be)
  • rsos from in silico dynamical modelling by tuning parameters for protein concentrations and other factors .r o y involved in the rate equations describing the systems. (lu.se)
  • Our results demonstrate compensatory mechanisms in the pancreatic transcription factor network due to mutant HNF1BS148L/+ protein. (harvard.edu)
  • In our first aim we will demonstrate how the replacement of GATA2 with GATA6 causes pathological mRNA and protein expression , utilizing ChIP - seq and RNA - seq to identify the genomic targets of the GATAs in healthy and diseased cells. (northwestern.edu)
  • Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. (biomedcentral.com)
  • Nuclear factor I/thyroid transcription factor 1 interactions modulate surfactant protein C transcription. (zju.edu.cn)
  • By recruiting more than thousand kindreds with early onset CAD and multiple metabolic risk factors for genetics and metabolic studies we have been able to map, identify and characterize a dozen of human disease genes for CAD and MetS, which have been reported in leading journals such as Nat Genet, Science, NEJM, Cell Metab, JCI, PNAS, AJHG, etc. (yale.edu)
  • Our studies will identify the specific genes directly governed by GATA2 in healthy tissue and GATA6 in diseased tissue. (northwestern.edu)
  • These included PTEN-dependent DNA damage repair in response to SHP2 inhibition, AKT-mediated bypass resistance in response to chronic SHP2 inhibition, and SHP2 control of hypoxia-inducible factor expression through multiple MAPKs. (aacrjournals.org)
  • TNDM is caused by mutations in the GATA6 gene in rare cases. (eurospe.org)
  • The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. (mpg.de)
  • Our data set includes regulators of ACTIVIN, BMP, fibroblast growth factor, and WNT signaling. (mpg.de)
  • Transcription factor CHF1/Hey2 regulates neointimal formation in vivo and vascular smooth muscle proliferation and migration in vitro. (jax.org)
  • However, they fail to differentiate to extraembryonic endoderm cells due to markedly reduced enhancer binding of GATA6, the lineage-determining transcription factor driving extraembryonic endoderm development. (nih.gov)
  • Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. (diagenode.com)
  • A) Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade CTCF antibody. (diagenode.com)
  • Chromatin Immunoprecipitation has been performed using chromatin from HeLa cells, the iDeal ChIP-seq kit for Transcription Factors and the Diagenode ChIP-seq-grade HDAC1 (A), LSD1 (B) and p53 antibody (C). The IP'd DNA was subsequently analysed on an Illumina ® Genome Analyzer. (diagenode.com)
  • In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.com)
  • in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor. (cellsignal.com)
  • thyroid transcription factor-1), is a tissue-specific transcription factor of the thyroid, lung, and ventral forebrain. (zju.edu.cn)
  • Eight of these SNPs were analyzed for transcriptional regulation using reporter assays where all tested regions exerted regulatory effects on transcription in epithelial and/or immune cell lines, and seven SNPs showed allelic differences. (biomedcentral.com)
  • Gene expression profiling of ErbB receptor and ligand-dependent transcription. (rochester.edu)
  • Here, we differentiated MODY5 human-induced pluripotent stem cells (hiPSCs) into pancreatic progenitors, and show that the HNF1BS148L/+ mutation causes a compensatory increase in several pancreatic transcription factors, and surprisingly, a decrease in PAX6 pancreatic gene expression. (harvard.edu)
  • This gene encodes a transcription factor that is important for the development of the hematopoietic, cardiac and gastrointestinal systems. (eurospe.org)
  • Moreover, we have shown that NF-B can also mediate muscle wasting by collaborating with STAT3 to markedly increase the transcription of inducible nitric oxide synthase (iNOS), an enzyme that catalyzes the conversion of L-arginine to citrulline resulting in the release of nitric oxide (NO)7,9,10,12. (sdfca.org)
  • Cardiovascular basic helix loop helix factor 1, a novel transcriptional repressor expressed preferentially in the developing and adult cardiovascular system. (jax.org)
  • The aberrant transcription of key oncogenes is driven by super-enhancers (SEs), which are characterized by large clusters of enhancers with significantly high density of transcription factors, cofactors, and epigenetic modulatory proteins. (ijbs.com)
  • Furthermore, the promoter upstream of exon 1 GATA6 (5-flanking area) includes an interferon consensus series binding proteins (ICSBP)-binding site and activator proteins-1 (AP-1)-binding site [11], while another promoter upstream of exon 2 (intron 1) has a PU.1-binding site [11]. (cancer-pictures.org)
  • For example, the concurrent signaling of interferon (IFN) and tumor necrosis factor (TNF) (IT) can synergistically elicit muscle wasting by stimulating the activity of transcription factors including STAT3 and the heterodimeric NF-B9C12. (sdfca.org)
  • Ventricular septal defect and cardiomyopathy in mice lacking the transcription factor CHF1/Hey2. (jax.org)
  • Mouse embryonic fibroblasts isolated from transgenic mice carrying doxycycline (Dox)-inducible Yamanaka factor constructs were used for reprogramming. (biomedcentral.com)
  • Our results indicate that direct and selective methylation of certain TFBS that prevents TF binding is restricted to special cases and cannot be considered as a general regulatory mechanism of transcription. (biomedcentral.com)
  • The core transcription regulatory loop of the digestive system tumors is complex and a high density of transcription regulatory complexes in the SEs and the crosstalk between SEs and the noncoding RNAs. (ijbs.com)
  • Since these factors are considered important for proliferation and differentiation, we also explored the possible alterations in their expression in gastrointestinal neoplasias. (tuni.fi)
  • MLL3 (KMT2C) and MLL4 (KMT2D), the major mammalian H3K4me1 methyltransferases, are critical for enhancer activation and cell-type-specific transcription during development and cell differentiation. (nih.gov)
  • Chemical factors such as soluble growth factors and insoluble components of the extracellular matrix are known to affect the differentiation of murine ESCs. (ox.ac.uk)
  • SEs are defined as large clusters of active enhancers in large clusters that are enriched with high levels of transcription factors (TFs), master co-factors, mediator complexes, RNA polymerase II (RNA pol II), and epigenetic modifications [ 5 , 7 ]. (ijbs.com)
  • A recognizable differ from C to G at placement ?137 altered the histone H4 gene-specific transcription factor-1 (H4TF-1) nuclear factor binding site [13] (Desk 1). (cancer-pictures.org)
  • In order to understand the development of adiposity, it is crucial to identify the factors and mechanisms that regulate the recruitment of mesenchymal stem cells (MSCs) of the vascular stromal fraction of the adipose tissue and its transformation into lipid-filled adipocytes. (biomedcentral.com)
  • We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
  • The disease affects up to 10% of reproductive age women, during what should be women's peak years of health, but an insufficient understanding of the factors that cause the disease has limited the development of new diagnostic and therapeutic approaches. (northwestern.edu)
  • Despite of the relevance of this issue, very little is known about the factors regulating the development of adipose tissue in fish, and the possible health alterations associated to an increased fat deposition. (biomedcentral.com)
  • as a proto-oncogene was recently characterized and the gene is implicated as a contributory factor in lung cancer development. (zju.edu.cn)
  • In this review, we summarize the role of this transcription factor in the development, diagnosis , and prognosis of lung cancer in the hope of providing insights into the utility of Nkx2-1 as a novel biomarker of lung cancer . (zju.edu.cn)
  • PFMs can be transformed to probabilistic models to construct position weight matrices (PWMs) or position-specific scoring matrices (PSSMs), which then can be used to scan any DNA sequence and predict transcription factors binding sites (TFBSs). (genereg.net)
  • The risk of lung cancer can be reduced by quitting smoking and by eliminating or reducing exposure to secondhand smoke and environmental and workplace risk factors. (aromabranding.co.id)
  • Because these factors function in other embryonic tissues I have established a powerful and reliable experimental cardiogenic assay with Xenopus explants, which allows investigating the role of these factors during early heart formation without interfering with other developmental processes. (xenbase.org)
  • We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. (diagenode.com)
  • Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the iDeal ChIP-seq kit for TFs (Cat. (diagenode.com)
  • Interleukin (IL)-18 was originally discovered as one factor that enhanced IFN- production from anti-CD3-stimulated Th1 cells, in the current presence of IL-12 specifically. (cancer-pictures.org)
  • This means that the database is non-redundant in the sense that there are not many models for the same factor. (genereg.net)
  • This is the main JASPAR collection and should be used when curated, non-redundant binding profile models for specific factors derived from experimental data are required. (genereg.net)
  • In light of these results, therapies that can disrupt multiple pathways or target redundant factors downstream of these humoral factors may be a more fruitful approach to combatting cachexia. (sdfca.org)
  • Dynamic transcription factor activity profiling in 2D and 3D cell cultures. (chicagobiomedicalconsortium.org)
  • The DNA fragment with the top-associated SNP 27:19,086,778 displayed the highest activity in keratinocytes with 11-fold induction of transcription by the risk allele versus 8-fold by the control allele (p difference = 0.003), and also mapped close (~3 kb) to an ENCODE skin-specific enhancer region. (biomedcentral.com)
  • The transcription activity of the gene promoter fragment showed that ?656 G/T (rs1946519), ?607 C/A (rs1946518), and ?137 G/C (rs187238) are in the promoter region which the other two SNPs are in the 5-untranslated region (Desk 1). (cancer-pictures.org)
  • It was also shown that a single differentially methylated CpG might affect transcription of the ESR1 gene [ 11 ]. (biomedcentral.com)
  • The importance of these factors during heart formation is further demonstrated in studies that shown that compound Gata4/Gata5 and Gata5/Gata6 mutants die embryonically or perinatally due to severe CHD. (xenbase.org)
  • HRT1, HRT2, and HRT3: a new subclass of bHLH transcription factors marking specific cardiac, somitic, and pharyngeal arch segments. (jax.org)
  • In short, whether or not the distinct methylation status of a single CpG affects specific transcription-related functions remains an open question. (biomedcentral.com)
  • Therefore, it is important to determine the factors that may affect the follow-up for diabetes. (eurospe.org)
  • The importance of these factors in heart formation has been highlighted in recent studies. (xenbase.org)
  • Thyroid transcription factor-1 is expressed in extrapulmonary small cell carcinomas but not in other extrapulmonarry neuroendocrine tumors. (zju.edu.cn)
  • Human gastrointestinal mucosa regenerates vigorously throughout life, but the factors controlling cell fate in mature mucosa are poorly understood. (tuni.fi)