Transactivator protein. Medical search. Frequent questions

In Drosophila, signal transduction from Wingless stabilizes cytosolic Armadillo, which then forms a bipartite transcription factor with the HMG-box protein Drosophila Tcf (dTcf) and activates expression of Wingless-responsive genes. (duke.edu)
Since inhibitors of protein synthesis are known to activate cellular signal transduction pathways, our findings demonstrate new mechanisms for the regulation of HSV-1 IE gene expression which may be important during latency and reactivation. (microbiologyresearch.org)

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. (nature.com)
In this review we discuss the current understanding of the signaling mechanisms used by TGF-beta to elicit its effects on target genes, focusing primarily on Smad proteins and their role in the transcriptional regulation of ECM gene expression. (nih.gov)
Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
The CaMKIIα-tTA transgene inserted on chromosome 12, resulting in a 508 kb deletion that affects five mouse genes: Vipr2 (vasoactive intestinal peptide receptor 2), Wdr60 (WD repeat-containing protein 60), Esyt2 (extended synaptotagmin-like protein 2), Ncapg2 (non-SMC condensin II complex, subunit G2), and Ptprn2 (protein tyrosine phosphatase, receptor type, N polypeptide 2). (alzforum.org)
The various zoological phyla have played with a toolbox of genes encoding receptors, adaptors, kinases, transactivators, and antimicrobial peptides according to their own agendas, essentially their own environments and pathogens," he said. (newswise.com)
Cellular DNA-binding proteins encoded by the sis gene ( GENES, SIS ). (nih.gov)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)

2004. Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor transcriptional activity in colorectal cancer cells. . (oregonstate.edu)
Nef protein of HIV-1 is a transcriptional repressor of HIV-1 LTR. (microbiologyresearch.org)
However, the proteins needed for transcriptional control have been reported to be immunogenic and there is often a substantial leakage in the systems (Clackson, 2000). (lu.se)

It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). (cancerindex.org)
Accumulation of unfolded proteins in the ER lumen triggers the dissociation of GRP78 from its quiescent UPR mediators. (molvis.org)

The recent identification of Smad proteins, TGF-beta receptor kinase substrates that translocate into the cell nucleus to act as transcription factors, has increased our understanding of the molecular mechanisms underlying TGF-beta action. (nih.gov)
CamKII-tTA transgenic mice are viable and fertile, with expression of the tetracycline-controlled transactivator protein (tTA) directed to forebrain neurons by the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter. (mmrrc.org)
Integrin-linked kinase (ILK) is a major structural adaptor protein governing signaling complex formation and cytoskeletal dynamics. (jneurosci.org)
Although the molecular mechanisms responsible for the selective vulnerability of these cells are not well understood, activation of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway has been implicated in neuroprotective responses to excitotoxicity in other neuronal populations. (jneurosci.org)
Along these lines, accumulating evidence shows that the p42/44 mitogen-activated protein kinase (MAPK) pathway plays an important role in neuronal cell survival. (jneurosci.org)
Our data reveal that the vulnerable cells express high levels of striatal-enriched protein tyrosine phosphatase (STEP), which is a key regulator of the extracellular signal-regulated kinase (ERK) pathway. (jneurosci.org)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
Casein kinase-2 mediated phosphorylation increases the SUMO-dependent activity of the cytomegalovirus transactivator IE2. (ncbs.res.in)

Tat contains a protein transduction domain (PTD), which is also known as a cell-penetrating peptide. (wikipedia.org)
This work aimed to clone, express, purify and evaluate the protective effect antioxidant of this enzyme on skin cells when fused to transactivator of transcription (TAT) protein transduction domain of HIV-1 and Abalone (Ab) peptides to allow cell penetration. (pimac.org)

The CamKII-tTA transgene was designed by Dr. Mark Mayford (Columbia University, New York) with 8.5 kb of the mouse CaMKIIalpha promoter placed upstream of the tetracycline-regulated transactivator (tTA or "Tet-Off") gene (flanked by an artificial intron and splice sites at the 5' end and by a polyadenylation signal from SV40 at the 3' end). (mmrrc.org)
The most commonly used systems are based on a transactivator in combination with a drug, e.g. the tetracycline-regulated system. (lu.se)

Tat protein also associates with RNA polymerase II complexes during early transcription elongation after the promoter clearance and before the synthesis of full-length TAR RNA transcript. (wikipedia.org)
This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. (cancerindex.org)
rTg4510 mice ("r" for regulatable) are produced by crossing the 4510 responder line, carrying a human MAPT P301L cDNA downstream of a tetracycline operon-responsive element (TRE), to an activator line expressing a tetracycline-controlled transactivator (tTA) under control of the CaMKIIα promoter. (alzforum.org)
Expression from the human cytomegalovirus major IE promoter, when cloned into the genome of HSV-1 mutants, was also increased by inhibition of protein synthesis. (microbiologyresearch.org)
Activation of the ICP0 promoter was observed when protein synthesis was blocked by alternative agents. (microbiologyresearch.org)
May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. (lu.se)
Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. (lu.se)
Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)

Tat is a regulatory protein that drastically enhances the efficiency of viral transcription. (wikipedia.org)
Like other lentiviruses, HIV-1 encodes a trans-activating regulatory protein (Tat), which is essential for efficient transcription of the viral genome. (wikipedia.org)
Human immunodeficiency virus Vpr product is a virion-associated regulatory protein. (microbiologyresearch.org)
Mechanism of action of regulatory proteins encoded by complex retroviruses. (microbiologyresearch.org)
In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. (abnova.com)
These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)

This locus, however, also encodes a protein from an alternative reading frame, designated p19ARF. (medscape.com)

For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)

We will discuss three key proteins that function as tumor suppressors implicated in the development of pediatric (and some adult) cancers: pRB, p53, and PTEN. (medscape.com)
RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
[ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)

Immunohistochemistry in diagnostic surgical pathology: contributions of protein life-cycle, use of evidence-based methods and data normalization on interpretation of immunohistochemical stains. (lsbio.com)

HBxAg is a transactivator of viral transcription that appears to contribute to HCC by altering patterns of host gene expression. (slideserve.com)

An analysis of the sera from mice immunized with both variants of the protein revealed that the mRBD expressed in CHO cells provides a significantly stronger humoral immune response compared with the RBD expressed in E.coli cells. (pimac.org)

Co-expression of specific inhibitory proteins promotes cell survival and growth. (ox.ac.uk)

STAT2 (AAH51284, 742 a.a. ~ 851 a.a) partial recombinant protein with GST tag. (abnova.com)
Antibody Reactive Against Recombinant Protein. (abnova.com)

Here, we report that high levels of the striatal-enriched protein tyrosine phosphatase (STEP), a key regulator of ERK/MAPK signaling, are found in vulnerable somatostatin-immunoreactive hilar interneurons. (jneurosci.org)

The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients. (wikipedia.org)
Specifically, TGF-beta isoforms have the ability to induce the expression of ECM proteins in mesenchymal cells, and to stimulate the production of protease inhibitors that prevent enzymatic breakdown of the ECM. (nih.gov)
Herpes simplex virus type 1 (HSV-1) transcription can be arrested at the immediate early (IE) stage by continuous treatment of cells with inhibitors of protein synthesis, usually cycloheximide, from the time of infection. (microbiologyresearch.org)
At the crux of the Hoffmann lab body of work is the Toll receptor, a protein that straddles the cell membranes of immune system "sentinel" cells, detecting molecular nametags of pathogens from the outside and transducing messages to the inside, activating and orchestrating defense against infection. (newswise.com)
In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)

The protein consists of between 86 and 101 amino acids depending on the subtype. (wikipedia.org)

The receptor-binding domain (RBD) of the protein S SARS-CoV-2 is considered to be one of the appealing targets for developing a vaccine against COVID-19. (pimac.org)

Here, we demonstrate that the endoplasmic reticulum stress sensor inositol-requiring enzyme 1 (IRE1α) and its substrate transcription factor X-box-binding protein 1 (XBP1) drive NK cell responses against viral infection and tumors in vivo. (cancerindex.org)

Protein synthesis inhibitors differentially superinduce c-fos and c-jun by three distinct mechanisms: lack of evidence for labile repressors. (microbiologyresearch.org)
Effect of protein synthesis inhibitors on growth factor activation of c- fos , c- myc , and actin gene transcription. (microbiologyresearch.org)

Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

The basic region is involved in RNA (TAR, trans-activation response element) binding and Tat proteins thus belong to the family of arginine-rich motif (ARM) RNA binding proteins. (wikipedia.org)
A five-residue motif for the design of domain swapping in proteins. (ncbs.res.in)

Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. (cancerindex.org)
These conditions include but are not limited to artery or vein occlusion in diabetic retinopathy, hypoxic retina in retinopathy of prematurity, aging in age-related macular degeneration, expression of mutant proteins in inherited retinal degeneration, traumatic injury leading to retinal detachment, and light sensitivity in the case of light-induced retinal degeneration. (molvis.org)
Effects of the human immunodeficiency virus type 1 Tat protein on the expression of inflammatory cytokines. (microbiologyresearch.org)
We have analysed the effect of cycloheximide on IE gene expression with HSV-1 mutants deficient in the production of functional levels of the three major transactivators, the virion protein (VP16) and two IE proteins (ICP0 and ICP4). (microbiologyresearch.org)
Expression from the HSV-1 IE promoters that control synthesis of ICP0 and ICP27 was, unexpectedly, stimulated by inhibition of protein synthesis. (microbiologyresearch.org)
Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. (microbiologyresearch.org)
Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis. (microbiologyresearch.org)
We also observed a clear increase in CTGF mRNA and protein expression levels in HBV-positive HCC human tissue samples in comparison with the HBV-negative controls, indicating a possible negatively associated relationship between miR-18a and CTGF. (medscimonit.com)
The choice of an expression system is essential when developing subunit vaccines, as it ensures the effective synthesis of the correctly folded target protein, and maintains its antigenic and immunogenic properties. (pimac.org)
Efforts have therefore, designed a study to compare the expression of green ¯uor- been made to develop systems based on different drugs or hormones escent protein (GFP) and GDNF under the control of the hGFAP including tetracycline, rapamycine and progestrone (Clackson, 2000). (lu.se)

We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts. (nature.com)

Ab-TrSOD, when compared to TrSOD, promoted a decrease in the formation of ROS of 19 and 14% at the concentrations of 1 and 6 μmol/L, respectively, indicating that this joplinks Recombinant Human Regulator of G-protein form was more effective in reducing oxidative stress compared to SOD without the cell penetrating peptide (CPP). (pimac.org)

A "no-hybrids" screen for functional antagonizers of human p53 transactivator function: dominant negativity in fission yeast. (ox.ac.uk)
We have developed a functional "no-hybrids" screen in the fission yeast Schizosaccharomyces pombe based on the transcription transactivator activity of human p53. (ox.ac.uk)

Factor de transcripción similar a Kruppel que contiene tres DEDOS DE ZINC CYS2-HIS2 en el extremo C y se une a una SECUENCIA RICA EN GC (caja GC) en los promotores ascendentes del gen. (bvsalud.org)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. (bdbiosciences.com)

Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. (thermofisher.com)

ILK forms an obligate heterotrimeric complex with particularly interesting Cys-His-rich protein (PINCH) and parvin, which together lend stability to the actin cytoskeleton at focal adhesions ( Brakebusch and Fässler, 2003 ). (jneurosci.org)
Amide temperature coefficients in characterizing the allosteric effects of ligand binding on local stability in proteins. (ncbs.res.in)

TTA proteins dimerize, and this dimer is capable of specifically binding tetracycline (or doxycycline, which we call dox, and also, these dimers can bind tetracycline operator sequences (called tet-o-secquences). (stanford.edu)
The molecular chaperone glucose-regulated protein 78 ( GRP78 ) or binding immunoglobulin protein (BiP), in addition to Ca 2+ binding and protein processing functions, possesses one more key role: master initiator of early UPR signaling. (molvis.org)
53BP1 (p53-binding protein 1) facilitates ATM-dependent DSB repair but is largely dispensable for ATM activation or checkpoint arrest. (birmingham.ac.uk)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

TP53 encodes the protein p53, which is known as the "guardian of the genome. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

What pathways are this gene/protein implicaed in? (cancerindex.org)

Human Health and Environmental Risk Assessment of OX5034 Aedes aegypti Containing Tetracycline-Repressible Transactivator Protein Variant (tTAV-OX5034, New Active Ingredient), DsRed2-OX5034 Protein (new inert ingredient), and the Genetic Material Necessary (Vector pOX5034) for Production of the Proteins in vivo . (ca.gov)

MHC-I and MHC-II proteins are key components of the antigen presentation machinery responsible for neoantigen presentation to CD8 + and CD4 + T lymphocytes, respectively. (portlandpress.com)
Therefore, by synthesizing the N gene sequence of SARS-CoV-2, constructing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in E.coli and obtained 15 mAbs against SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established. (pimac.org)

Biosantech has developed a novel vaccine called Tat Oyi, which aims at the Tat protein. (wikipedia.org)

Anatomy16

Organisms32

Diseases5

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes76

Information Science3

Signal Transduction2

Genes7

Transcriptional3

Accumulation of unfolded proteins2

Kinase8

Transduction domain2

Tetracycline-regulated2

Promoter8

Regulatory6

Encodes a protein1

Chromosome1

Tumor3

Immunohistochemistry1

Viral transcription1

Mice1

Inhibitory1

Recombinant Protein2

Tyrosine phosphatase1

Cells5

Amino acids1

Receptor1

Endoplasmic reticulum1

Inhibitors2

Transcription Factors1

Motif2

Expression12

Cellular1

Regulator1

Functional2

Zinc2

Sodium azide1

Dimerization1

Stability2

Binding4

TP532

Cell2

Pathways1

Human1

Antigen2

Vaccine1