• In the presence of BFA, a mannose-6-phosphate receptor (M6PR)-enriched tubular network rapidly forms from the TGN, not from the prelysosomal compartment, and can be labeled with endocytic tracers after only 5 min of uptake at either 20 degrees C or 37 degrees C, indicating that it is also functionally an early endosome. (nih.gov)
  • The RabA4b GTPase labels a novel trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing root hair cells. (lecollege.org)
  • Syndapin II can associate with Golgi membranes and this association increases upon Golgi exit block. (mdc-berlin.de)
  • An excess of the syndapin SH3 domain strongly inhibited budding from Golgi membranes in vitro. (mdc-berlin.de)
  • Importantly, a cytosolic fraction containing endogenous syndapin-dynamin complexes was sufficient to promote vesicle formation from Golgi membranes in a syndapin-dependent manner. (mdc-berlin.de)
  • Interestingly, acute depletion of phosphatidylinositol 4-phosphate (PtdIns4P) at the Golgi complex reduced GAP-43 membrane binding, highlighting a new, pivotal role for this anionic lipid and supporting the idea that basic amino acid residues are involved in the electrostatic interactions between GAP-43 and membranes of the Golgi complex where they are S-acylated. (portlandpress.com)
  • Golgi membranes form multilayer stacks that are laterally linked into a ribbon. (frontiersin.org)
  • We have demonstrated that nutrient-deprived β cells deliver newly synthesized secretory granules (SGs) to lysosomes in the Golgi area, where degradation of their cargo (proinsulin and insulin) provides necessary nutrients leading to mTOR-mediated suppression of macroautophagy. (nature.com)
  • Upon dissociation, the lysosomal enzymes are released into the lysosomes, and IGF2R either returns to the Golgi to repeat this process or distributes on the cell surface to internalize IGF2. (sciencemag.org)
  • Targeting of glycoprotein I (gE) of varicella-zoster virus to the trans-Golgi network by an AYRV sequence and an acidic amino acid-rich patch in the cytosolic domain of the molecule. (asm.org)
  • Previous studies suggested that varicella-zoster virus (VZV) envelope glycoproteins (gps) are selectively transported to the trans-Golgi network (TGN) and that the cytosolic domain of gpI (gE) targets it to the TGN. (asm.org)
  • ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65," Journal of Cell Biology , vol. 182, no. 5, pp. 837-843, 2008. (hindawi.com)
  • Syndapins are crucial for Golgi vesicle formation because antisyndapin antibodies, used either in in vitro reconstitutions or in living cells, inhibited this process. (mdc-berlin.de)
  • Antebi A, Fink GR (1992) The yeast Ca(2 +)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. (springer.com)
  • Function of Golgi-centrosome proximity in RPE-1 cells. (bioportfolio.com)
  • However, most of these conditions have not only separated these two organelles, but also caused extensive fragmentation of the Golgi, making it difficult to dissect the specific contribution of Golgi-centrosome proximity. (bioportfolio.com)
  • These results suggest that Golgi-centrosome proximity per se is not necessary for the normal function of RPE-1 cells. (bioportfolio.com)
  • GOLPH3 Bridges Phosphatidylinositol-4- Phosphate and Actomyosin to Stretch and Shape the Golgi to Promote Budding," Cell , vol. 139, no. 2, pp. 337-351, 2009. (hindawi.com)
  • These interactions describe fundamental steps in retromer-mediated transport and establish that the spatial organization of the retromer network is a critical element required for efficient retromer-mediated sorting. (aston.ac.uk)
  • Thus, morphological and subsequent functional impairments of the Golgi may contribute to the neurotoxicity associated with neurodegenerative diseases. (frontiersin.org)