• Usually available commercially with sepharose as the supporting matrix in the form of packed columns. (wikipedia.org)
  • The small particle size (34 μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. (sigmaaldrich.com)
  • HisTrap ™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose ™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). (sigmaaldrich.com)
  • Ni Sepharose ™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. (sigmaaldrich.com)
  • The high flow rates made possible by the Ni Sepharose ™ 6 Fast Flow matrix make HisTrap ™ FF columns well suited for small scale-up. (sigmaaldrich.com)
  • The commonly used supporting matrix would be cross-linked agarose (sepharose), sephadex, polyacrylamide, and silica. (wikipedia.org)
  • HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification. (sigmaaldrich.com)
  • GSTrap ™ FF columns are prepacked Glutathione Sepharose ™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins. (sigmaaldrich.com)
  • The medium, Glutathione Sepharose ™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC ™ System. (sigmaaldrich.com)
  • After purification by Ni-Sepharose affinity chromatography, the Tα1-Intein fusion protein was induced by the thiols β-mercaptoethanol or d,l-dithiothreitol, or by increasing the temperature, to release Tα1 through intein-mediated N-terminal cleavage. (biomedcentral.com)