CryopreservationCryoprotectantsDimethyl sulfoxideVitrificationCPAsGlycerolDeep eutectic solventsDMSOAntineoplastic AgentsCryoprotectantThermalEfficacyViabilityPathogensAnticarcinogenic AgentsMetabolismInhibitsTherapeuticCellularAntibacterial agentsLiverStemChemopreventiveOrganSolutionsChemicalMolecular weightProtectDevelopmentReduceRequiresHumansWorkSafetyCurrentWater
Cryopreservation7
- Successful cryopreservation requires the addition of cryoprotective agents to protect against freezing damage and dehydration. (rsc.org)
- For decades, cryopreservation has largely relied on the same two primary agents: dimethylsulfoxide and glycerol. (rsc.org)
- This is one of the first times that this class of solvents has been systematically tested for cryopreservation of mammalian cells and as such this research opens the way for the development of potentially thousands of new cryoprotective agents that can be tailored to specific cell types. (rsc.org)
- Dimethyl sulfoxide (DMSO) has been the standard cryopreservation agent for freezing cells since the 1960 s 2 . (nature.com)
- These findings further highlight the importance of using multicomponent systems to reduce toxicity and improve the long-term viability of cryopreservation rather than single cryoprotective agents. (tomorrow.bio)
- We have studied the tolerance of spermatophores from the oceanic squid Illex coindetii to several cryoprotectants, in two toxicity experiments and a cryopreservation test. (ualg.pt)
- The application of these techniques to rooster spermatozoa is reviewed in relation to specific freezing techniques, the effects of cryoprotective agents (CPAs) and extenders, and the determination of spermatozoa quality after cryopreservation. (cryoletters.org)
Cryoprotectants2
- Cryoprotectants, also known as cryoprotective agents or CPAs, are a type of medical-grade antifreeze introduced through a process called perfusion. (tomorrow.bio)
- Lead researcher, Dr. Saffron Bryant, and his team at RMIT University found that using deep eutectic solvents as cryoprotectants reduces toxicity and improves the outcome of cryoprotectant solutions on cellular viability post-thaw. (tomorrow.bio)
Dimethyl sulfoxide5
- Dimethyl sulfoxide (DMSO) is the most widely used cryoprotective agent (CPA) today, but due to indications of cellular toxicity, changes of the cellular epigenetic state, and patient-related side effects, there is an increasing demand for DMSO-free alternatives. (lu.se)
- This freeze media should contain a cryoprotective agent such as dimethyl sulfoxide (DMSO) or glycerol. (strexcell.com)
- The dimethyl sulfoxide solution of sodium cyanide can cause cyanide poisoning through skin contact while dimethyl sulfoxide itself has a relative low toxicity. (chemicalbook.com)
- dimethyl sulfoxide is widely applied as the extraction agent in a lot of chemical and pharmaceutical companies. (chemicalbook.com)
- Therefore, the recycling of dimethyl sulfoxide has become a bottleneck as its further wide application as extraction agent. (chemicalbook.com)
Vitrification3
- These factors and mechanisms (or hypotheses) of cryoinjury and its prevention are discussed, including the most famous "two factor hypothesis" theory of Peter Mazur, concepts of cold shock, vitrification, cryoprotective agents (CPAs), lethal intracellular ice formation and osmotic injury during the addition/removal of CPAs and during the cooling/warming process as well as modeling/methods in the cryobiological research. (iranjournals.ir)
- Toxicity of the vitrification media is also reduced by sucrose because it probably reduces the necessary amount of intracellular cryoprotectant (Kasai 1996). (globalmedgroup.com)
- Additionally there is no requirement of step wise addition and removal of Crypprotective agent or Vitrification media. (frozencell.org)
CPAs1
- One of the main barriers to this is insufficient time for CPAs to penetrate the cells prior to toxicity. (tomorrow.bio)
Glycerol3
- A deep eutectic solvent made from proline and glycerol was an effective cryoprotective agent for all four cell types tested, even with extended incubation prior to freezing. (rsc.org)
- In this study, six DESs were explored for their cryoprotective capacity towards mammalian cells, which included various ratios of mixtures of galactose (Gal) or glycerol (Gly) with either choline chloride (ChCl), betaine (Bet), or proline (Prol) [4]. (tomorrow.bio)
- First are intracellular cryoprotective agents like glycerol and dimethylsulfoxide. (iranjournals.ir)
Deep eutectic solvents1
- Six deep eutectic solvents were explored for their cryoprotective capacity towards mammalian cells. (rsc.org)
DMSO1
- Frozen permanent cultures of the tester strains were prepared from fresh, overnight cultures to which DMSO was added as a cryoprotective agent. (europa.eu)
Antineoplastic Agents1
- Antineoplastic agents that are used to treat hormone-sensitive tumors. (lookformedical.com)
Cryoprotectant2
- In order to reduce the risk of ice crystal formation, cryoprotectant agents are used. (tomorrow.bio)
- This study does, however, give insight as to the potential for thousands of potentially new cryoprotectant agents, which may eventually result in improved technologies and processes that could revolutionize the way we view Biostasis . (tomorrow.bio)
Thermal3
- The solvents were tested for their thermal properties, including glass transitions, toxicity, and permeability into mammalian cells. (rsc.org)
- They were tested for their toxicity, thermal behavior, glass transition, and, ultimately, their cryoprotective capacity against four different human cell types, including skin and brain cells [4]. (tomorrow.bio)
- It has low toxicity, good thermal stability, and is not miscible with paraffin. (chemicalbook.com)
Efficacy1
- Several experimentally proven chemopreventive agents have been observed to lack efficacy with and without toxicity. (brjnmims.org)
Viability1
- Our IRIs permit the use of lower amounts of conventional Cryoprotective Agents (CPA) resulting in higher post-thaw viability and recovery. (pantheracryo.com)
Pathogens1
- Many pathogens are susceptible to CO toxicity, therefore expressing non-CO producing heme degradation enzymes evades self-inflicted toxicity while meeting nutritional iron needs. (wikipedia.org)
Anticarcinogenic Agents3
- Anticarcinogenic Agents" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
- This graph shows the total number of publications written about "Anticarcinogenic Agents" by people in this website by year, and whether "Anticarcinogenic Agents" was a major or minor topic of these publications. (rush.edu)
- Below are the most recent publications written about "Anticarcinogenic Agents" by people in Profiles. (rush.edu)
Metabolism2
- The Minnesota group has suggested that the toxicity of the oxygen inhibits metabolism by literally poisoning the processes which maintain life to allow prolonged cold storage. (biostasis.com)
- Tamoxifen acts as an anti-estrogen (inhibiting agent) in the mammary tissue, but as an estrogen (stimulating agent) in cholesterol metabolism, bone density, and cell proliferation in the ENDOMETRIUM. (lookformedical.com)
Inhibits1
- Suspensive agent: inhibits aggregation of dust by keeping it disaggregated. (pdfplayer.org)
Therapeutic1
- The administration of therapeutic agents drop by drop, as eye drops, ear drops, or nose drops. (lookformedical.com)
Cellular4
- Unfortunately, both of these agents are toxic to a degree, thus additional damage would need to be repaired before the warming of complex cellular organisms. (tomorrow.bio)
- 2000). More in detail, addition of such non-permeating agents results in a hyperosmotic extracellular solution, promoting cellular dehydration and diminution of ice crystal formation (Shaw and Jones 2003). (globalmedgroup.com)
- The Influence of Pathological Mutations and Proline Substitutions in TDP-43 Glycine-Rich Peptides on Its Amyloid Properties and Cellular Toxicity. (bio-rad-antibodies.com)
- Cellular responses to cancer chemopreventive agent D,L-sulforaphane in human prostate cancer cells are initiated by mitochondrial reactive oxygen species. (rush.edu)
Antibacterial agents1
- These results provide opportunities to develop Cr-SPs as natural antioxidant and antibacterial agents. (brjnmims.org)
Liver3
- Successful chemopreventive agents include selective oestrogen receptor modulators and aromatase inhibitors for breast cancer, the 5-α-reductase inhibitors for prostate cancer, non-steroidal antiinflammatory drugs (NSAIDs) for colorectal lesions and vaccines for viruses that are associated with cervical and liver cancers. (brjnmims.org)
- Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals. (in.ua)
- Ibuprofen with its short circulation half-life (1.8-2.0 h) and its lack of a pathologic metabolite, showed a low reported rate of liver toxicity compared with other NSAIDs ( Ref ). (phrmahealth.com)
Stem1
- Pediatric cancer treatments such as chemotherapy have recognized toxicity on germline stem cells, which could lead to infertility at adulthood ( 1 , 2 ). (elifesciences.org)
Chemopreventive1
- Inhaled isotretinoin (13-cis retinoic acid) is an effective lung cancer chemopreventive agent in A/J mice at low doses: a pilot study. (rush.edu)
Organ1
- Furthermore, these two agents are ineffective for hundreds of cell types, and organ and tissue preservation has not been achieved. (rsc.org)
Solutions1
- Cryoprotective solutions have been supplemented with macromolecules such as sucrose and human serum albumin (HSA). (globalmedgroup.com)
Chemical2
- This allows for extensive fine-tuning of chemical structures for unique applications without the risk of toxicity. (tomorrow.bio)
- On the other hand, slow cooling rates may result in chemical toxicity due to higher salt concentrations. (strexcell.com)
Molecular weight2
- The ideal cryoprotector should have a low molecular weight with high water solubility and low toxicity. (iranjournals.ir)
- Low molecular weight organic compounds tend to have melting points lower than water, which makes them suitable for use as antifreeze agents. (wikipedia.org)
Protect1
- Most if not all commercial antifreeze formulations intended for use in heat transfer applications include anti-corrosion and anti- cavitation agents (that protect the hydraulic circuit from progressive wear). (wikipedia.org)
Development1
- Specifically, these findings could eventually lead to the further development of new cryoprotective agents that could be tailored to target specific cell types [4]. (tomorrow.bio)
Reduce1
- Agents that reduce the frequency or rate of spontaneous or induced tumors independently of the mechanism involved. (nih.gov)
Requires1
- Their toxicity also requires that they be administered at near-freezing temperatures. (tomorrow.bio)
Humans2
- The current review focuses on several approaches and agents that have been scrutinized by way of randomized clinical trials in humans for their cancer prevention potential. (brjnmims.org)
- They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (lookformedical.com)
Work2
- How do cryoprotective agents work? (tomorrow.bio)
- Moreover disadvantage of high concentration of Cryo- protective agent does not work here. (frozencell.org)
Safety1
- Ibuprofen offers a favorable safety profile compared with other NSAID agents. (phrmahealth.com)
Current1
- Their results support the current practice of combining cryoprotective agents . (tomorrow.bio)
Water3
- Complexes of iodine and non-ionic SURFACE-ACTIVE AGENTS acting as carrier and solubilizing agent for the iodine in water. (lookformedical.com)
- Because of this property, lecithin may bind water and oil, which are generally incompatible, making it an emulsifying agent [16]. (pdfplayer.org)
- Wetting agent: aids in the faster dissolution of dusty particles in water. (pdfplayer.org)