Total genomic library. Medical search. Frequent questions

These are due to the advance of genome-wide association studies from the ability to create and sequence genomic libraries. (wikipedia.org)
Below are the steps for creating a genomic library from a large genome. (wikipedia.org)
Since the genome of an organism is fixed, chromosomal DNA may be isolated from almost any cell type in order to prepare genomic DNA. (biologydiscussion.com)
By digesting complex genomic DNA from an organism it is possible to reproducibly divide its genome into a large number of small fragments, each approximately the size of a single gene. (biologydiscussion.com)
Our DNA services includes whole genome, whole exome or targeted library preparation and sequencing on Illumina platforms. (lu.se)
Xq28 appears to be an unstable region of the human genome and genomic rearrangements are recognised as major causes of two single gene defects, haemophilia A and incontinentia pigmenti, which map within Xq28. (bmj.com)
The generation of a library of PCR-analyzed microsatellite variants for genetic mapping of the mouse genome. (ox.ac.uk)
Estimates of genome size based on k-mer frequency analysis were highly similar to the total size of the finished genome, suggesting that the entirety of the genome was recovered. (authorea.com)
The Indian male's genome was sequenced at 38.6 X coverage with a total of 110 Gb of sequence data generated using the Illumina HiSeq 2000 platform. (ijpsonline.com)
To facilitate M. grisea genome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC) library from the rice infecting strain 70-15. (johnshopkins.edu)
These data show that the BAC library is suitable for genome analysis of M. grisea. (johnshopkins.edu)
Surveillance consists of sample acquisition, library preparation, and whole genome sequencing. (cdc.gov)
Christopher Douse explores the repetitive portion of the human genome, so-called 'genomic dark matter', and its role in human brain development and degeneration. (lu.se)
This award includes a $1.2 million grant to support his lab's exploration of the repetitive portion of the human genome, so-called 'genomic dark matter', and its role in human brain development and degeneration. (lu.se)

In this pilot study, we sought to identify clinical and molecular factors that distinguish HGSOC patients who share similar clinical characteristics and pathology at diagnosis with exceptional survival outcomes, either LT or ST, through integrated analysis of clinical features, germline variants, somatic genomic alterations, and tumor immune microenvironment. (biomedcentral.com)
The newly identified structural variants will facilitate genetic improvement of tomato through both marker-assisted selection and genomic selection. (nature.com)
As new variants emerge, DoD medical and public health practitioners should maximize the use of genomic surveillance resources within DoD to inform force health protection measures. (health.mil)

Genomic characterization predicted specific genomic islands highly associated with virulence, transcriptional regulation, and DNA restriction-modification systems. (frontiersin.org)

Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. (biomedcentral.com)
The loss of the expression of the maternal allele of the UBE3A gene is typically associated with the four following mechanisms: Deletion at the 15q11.2-q13 locus, UBE3A functional loss variation, presence of paternal parthenogenetic double chromosome or genomic imprinting defect ( 4 ). (spandidos-publications.com)

Nanopore MinION device at Instituto Nacional Investigação em Saúde (INIS), Ministry of Health of Angola, Luanda, on the AO-1 isolate, as part of the ArboSPREAD project focused on genomic surveillance of arthropod-borne viruses. (cdc.gov)
Establishment of SARS-CoV-2 Genomic Surveillance Within the Military Health System During 1 March-31 December 2020. (health.mil)
This report describes SARS-CoV-2 genomic surveillance conducted by the Department of Defense (DoD) Global Emerging Infections Surveillance Branch and the Next-Generation Sequencing and Bioinformatics Consortium (NGSBC) in response to the COVID-19 pandemic. (health.mil)
Genomic surveillance supports the development of diagnostics, therapeutics, and vaccines and can help monitor their effectiveness over time. (health.mil)
In the current case-control study, Salari and colleagues used a total of 581 samples - 333 samples for classifying disease and developing algorithms for initial diagnosis, surveillance, and molecular-grade prediction, as well as 248 samples for blinded validation. (medscape.com)
Genomic insights are an important component of the response to COVID-19, and continuing genomic surveillance is warranted. (who.int)

The main consideration in the construction of genomic or cDNA libraries is, therefore, the nucleic acid starting material. (biologydiscussion.com)

A genomic library is a collection of overlapping DNA fragments that together make up the total genomic DNA of a single organism. (wikipedia.org)
In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. (wikipedia.org)
The vector containing the inserted fragments of genomic DNA can then be introduced into a host organism. (wikipedia.org)
In addition, several fosmids clearly belonging to Group II Euryarchaeota were retrieved and recruited many fragments from the total direct DNA sequencing suggesting that this group might be quite abundant in this habitat. (nih.gov)
pastoris, thus unexpectedly providing a feasible method to obtain large genomic fragments from other less prevalent members of this community. (nih.gov)
A DNA library is a set of cloned fragments that collectively represent the genes of a particular organism. (biologydiscussion.com)
This means that digesting genomic DNA with EcoRI, which recognizes the sequence 5′-GAATTC-3′, will produce fragments each of which is, on average, just over 4 kb. (biologydiscussion.com)
A new method was developed for separation of partially digested large-molecular-weight DNA fragments that facilitated library construction with large inserts. (johnshopkins.edu)

Genomic and transcriptomic sequencing analyses were performed with phenotypic validation to investigate its adaptation in SARS-CoV-2 infected environment. (frontiersin.org)
Microbiomic studies using the next generation sequencing (NGS) technology query sequence abundance of a single gene (e.g., 16S rRNA or ITS) or multiple genes (e.g., meta-genomic/meta-transcriptomic) in the samples and provide abundance information for hundreds to thousands of microbial genera or species. (forsyth.org)

The window of opportunity to tailor therapeutic interventions to control progressive disease is limited due to the inherent cellular heterogeneity and genomic instability of HGSOC. (biomedcentral.com)
This patient therefore provides further evidence for the instability of this genomic region. (bmj.com)

Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. (wikipedia.org)
Library complexity is assessed using qPCR prior to amplification. (utmb.edu)

RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). (utmb.edu)
ChIP DNA libraries were made following the Illumina ChIP-seq library preparation kit. (nih.gov)
ChIP-Seq signal was quantified as total number of reads per million in the region of interest. (nih.gov)

Forty-three sequences containing simple sequence repeats or microsatellites were generated from an M13 library of total genomic mouse DNA. (ox.ac.uk)

7 Genomic data have also become critical components of epidemiology and outbreak response. (health.mil)
These bioinformatic tools are means for major actionable results: maintaining quality assurance and checks, defining population structure, performing genomic epidemiology, and inferring lineage to allow reliable and actionable identification and classification. (cdc.gov)
We undertook an integrated analysis of genomic and epidemiological data to investigate a large health-care- associated outbreak of coronavirus disease 2019 (COVID-19) and to better understand the epidemiology of COVID-19 cases in Tasmania, Australia. (who.int)

Particular genes can be isolated from DNA libraries, much as books can be obtained from conventional libraries. (biologydiscussion.com)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)

Our analyses revealed a 'great divide' genomic boundary extending from the Black Sea to the Baltic. (lu.se)

Loci were isolated from a genomic library enriched for AC repeats. (researchgate.net)
It's no exaggeration to say that we're at the dawn of a new genomic era in which the systematic study of repeats and their functions is possible," says Christopher Douse. (lu.se)

Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. (wikipedia.org)
Construction of a genomic library involves creating many recombinant DNA molecules. (wikipedia.org)
These recombinant molecules are taken up by a host bacterium by transformation, creating a DNA library. (wikipedia.org)

The urinary comprehensive genomic profiling (uCGP) assay (UroAmp, Convergent Genomics) had a specificity of 90% and sensitivity of 95% for an initial diagnosis of urothelial carcinoma in patients with hematuria - identifying 95 of 100 people with urothelial carcinoma and 90 of 100 patients without the disease. (medscape.com)

A reporter-gene-fusion-based genomic library that was custom-generated in this study enabled the detection of 23 secretory proteins. (cdc.gov)

Methods SARS-CoV-2 genomic sequences were generated from positive samples collected, along with epidemiological metadata, at a walk-up, rapid testing site in the Mission District of San Francisco, California during November 22-December 2, 2020 and January 10-29, 2021. (medrxiv.org)
In Australia, integration of genomic sequencing METHODS into the response to COVID-19 has al owed clusters and outbreaks to be identified and transmission chains to COVID-19 cases notified to the Tasmanian Department of be rapidly detected. (who.int)

These results indicate that astrocyte response to elevated dopamine involves PARP1-mediated CTCF genomic restructuring and concerted expression of gene networks. (jneurosci.org)

Certain viral lineages bearing spike mutations, defined in part by L452R, S13I, and W152C, comprised 54.9% of the total sequences from January, compared to 15.7% in November. (medrxiv.org)

Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available to probe its biology. (asm.org)

We demonstrate its use in capturing missing heritability in GWAS, providing insights into a classical genetics problem and facilitating genomic breeding (Extended Data Fig. 1 ). (nature.com)

Genomic libraries are commonly used for sequencing applications. (wikipedia.org)
Sanger and his team of scientists created a library of the bacteriophage, phi X 174, for use in DNA sequencing. (wikipedia.org)
The library was loaded onto a flow cell (FLO-MIN106) and sequencing data were collected for 24 hours. (cdc.gov)
The comparison between the direct and fosmids sequencing revealed a bias in the fosmid libraries against low-GC DNA and specifically against the two most dominant members of the community, Candidatus Pelagibacter and P. marinus subsp. (nih.gov)
Meta-genomic sequencing for total DNA isolated from samples. (forsyth.org)
By coupling sequencing and pairwise competition experiments, we demonstrated that the first mutation λ evolved en route to the innovation would only evolve in the presence of the ancestral host, whereas later steps in λ's evolution required the shift to a resistant host. (elifesciences.org)
Additionally, the pandemic has required public health laboratories to reach high throughput proficiency in sequencing library preparation and downstream data analysis rapidly. (cdc.gov)

We demonstrate a temporally dynamic pattern of genomic plasticity that triggers pronounced changes in astrocyte morphology and function. (jneurosci.org)

Here, we report a comprehensive secretome (600 proteins) of this species, which was identified using a multipronged strategy based on genetic/genomic, proteomic, and bioinformatic approaches. (cdc.gov)

Cobalt (300 µM) alone treatment drastically inhibited the stomatal conductance, plant height (45%), leaf area (30%), free amino acid (139%), relative electrolyte leakage (109%), and total soluble sugars (71%), compared with the control. (bvsalud.org)

The choice of the particular type of gene library depends on a number of factors, the most important being the final application of any DNA fragment derived from the library. (biologydiscussion.com)

4-6 Locally, genomic shown to be beneficial in investigating health-care- analysis has been used to analyse transmission routes, associated infections, 9,17 monitoring community trans- assign likely origins of infection, link outbreak cases and mission, 8-10 informing public health responses 9,10,18 and inform public health interventions and policies. (who.int)

Besides the chemistry sets under the Christmas tree - there would be a total of seven, in increasing complexity - a secondary school teacher, who was also an amateur mineralogist, played an important role. (lu.se)

National Library of Medicine The National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information. (cnos.net)

Thus, it is important to consider carefully the cell or tissue type from which the mRNA is to be deriver in the construction of cDNA libraries. (biologydiscussion.com)

Hybridization of total genomic DNA to the library and analysis of individual clones indicated that ~26% of the clones contain single-copy DNA. (johnshopkins.edu)
Genomic analysis provided useful additional information on COVID-19 in Tasmania, including evidence of a large health-care-associated outbreak linked to an overseas cruise, the probable source of infection in cases with no previously identified epidemiological link and confirmation that there was no identified community transmission from other imported cases. (who.int)
9 Genomic data enhance national Health between 2 March and 15 May 2020 were included surveil ance data by clarifying the source of infection in in the analysis. (who.int)

We used 250 ng of total DNA input in the library preparation. (cdc.gov)
DNA should be of high quality and diluted in water or EB buffer, presence of EDTA in the storage buffer might affect the library preparation. (lu.se)

Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. (lu.se)

In Arabidopsis , as giant single epidermal cells, trichomes have become an excellent model system to study cell differentiation and development because it is easy to analyze them at the genetic, genomic and cell biology levels. (biomedcentral.com)

This allows researchers to also determine the percentage of infectious viral particles actually carrying a fragment of the library. (wikipedia.org)

and a cDNA library, which represents the mRNA from a cell or tissue at a specific point of time. (biologydiscussion.com)
However, if the goal is the production of new or modified proteins, or the determination of tissue-specific expression of timing patterns, cDNA libraries are more appropriate. (biologydiscussion.com)
In contrast, however, cDNA libraries represent only mRNA being produced from a specific cell type at a particular time in the cell's development. (biologydiscussion.com)

Costs are reduced by indexing ("bar coding") individual template libraries, allowing multiple libraries to be sequenced in each of the flow cell lanes. (utmb.edu)

Several unlinked single-copy DNA probes were used to screen 4608 clones in the library and an average of 13 (minimum of 6) overlapping BAC clones was found in each case. (johnshopkins.edu)

Similar turnovers occurred in western Siberia, where we report new genomic data from a 'Neolithic steppe' cline spanning the Siberian forest steppe to Lake Baikal. (lu.se)

We also generated multiple resources useful for future genomic research on Lake Trout including a repeat library and a sex averaged recombination map. (authorea.com)

By doing this test, researchers can also determine the efficiency of the ligation and make adjustments as needed to ensure they get the desired number of clones for the library. (wikipedia.org)
A genomic library from Chinese Spring' (CS) was screened with 5 trinucleotide probes and about 1.1% of the clones contained microsatellites. (usda.gov)
The library was used to identify BAC clones containing a adenylate cyclase gene (mac1). (johnshopkins.edu)

Up to eight samples/libraries are pooled prior to the enrichment step. (lu.se)

A similar method can be used to titer genomic libraries made with non-viral vectors, such as plasmids and BACs. (wikipedia.org)

Anatomy9

Organisms29

Diseases3

Chemicals and Drugs58

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes109

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture6

Information Science29

Named Groups1

Health Care5

Genome14

Variants3

Characterization1

Chromosome3

Surveillance6

Nucleic acid1

Fragments8

Transcriptomic2

Instability2

Amplification2

ChIP-Seq3

Sequence1

Epidemiology3

Genes3

Analyses1

Repeats2

Molecules3

Genomics1

Detection1

Methods2

Involves1

Sequences1

Probe1

Insights1

Sequencing7

Morphology1

Approaches1

Soluble1

Gene Library1

Inform1

Complexity1

Advances1

Construction1

Analysis3

Preparation2

Large1

Study1

Fragment1

Specific3

Cell1

Single1

Report1

Resources1

Clones3

Samples1

Made1