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Fluorescence Polarization ImmunoassayFluorescence PolarizationFetal Organ MaturityFluorescent DyesSpectrometry, FluorescenceImmunoassayEnzyme Multiplied Immunoassay TechniqueHigh-Throughput Screening AssaysAmniotic FluidSphingomyelinsBrucellosisDigoxinSolanineChromatography, High Pressure LiquidAutoanalysisBlood StainsPhenytoinFluorescenceDiphenylhexatrieneProtein BindingSubstance Abuse DetectionReagent Kits, DiagnosticGentamicinsRadioimmunoassayVancomycinQuality ControlCyclosporinsGas Chromatography-Mass SpectrometryTeicoplaninImmunoenzyme TechniquesDrug MonitoringMembrane FluidityBiological AssayHomocysteineMicroscopy, FluorescenceFluoresceinsFalse Negative ReactionsTheophyllineMicroscopy, PolarizationCross ReactionsSensitivity and SpecificityCyclosporineReproducibility of ResultsFluoresceinFluorescent Antibody TechniqueRhodaminesLigandsRadioligand AssayEncyclopedias as TopicBinding, CompetitiveBinding SitesMolecular Sequence Data
FPIADetectionHigh throughputMicroplate ReaderIntra-assayPrincipleQuantitativeQuantificationAnalyzerHomocysteineLigandTranswellLuminescence2020ELISAMeasurementKinetic experimentsProtein-protein intVitroInteractionsRadioactiveDeterminationQuantitateAntibodiesPhosphorylationAnalyzeMembraneProteinsCellsRESULTSRecognitionExtensivelyMETHODSMethodDemandsCellDevicePlateConcentrationMoleculesLevelsDevelopmentHttpsProvideCompetitiveFormatTargetDrugsRangeTherapeuticAlternativeObvious
FPIA6
Detection7
  • 1514 Eisenhower Place Ann Arbor, MI 48108-3284, USA Arbor Assays builds the highest quality detection and immunoassay products for clinically important biomolecules. (contractresearchmap.com)
  • A detection method is used to determine the presence and extent of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. (wikipedia.org)
  • The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
  • The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
  • Beckman Coulter Life Sciences' Valita Titer assays range are rapid, high throughput IgG quantification assays which use fluorescence polarization (FP) for detection. (mybeckman.ca)
  • That's why at Tecan we offer detection solutions that have been optimised for four areas: genomics & proteomics, microbiology, cell-based assays, and drug discovery. (tecan.com)
  • The use of increasingly complex assays and more advanced cellular models is placing greater demands on the readouts and detection methods required to study them. (tecan.com)
High throughput1
  • This feature along with short assay runtime makes this method a useful platform for the development of high throughput assays to determine modular domain-ligand interactions which could have wide-ranging applications in both basic and translational cancer research. (biomedcentral.com)
Microplate Reader1
  • Here the PHERAstar ® FS HTS microplate reader is used in a time-resolved fluorescence assay to study the loss of membrane integrity. (bmglabtech.com)
Intra-assay2
  • At mean levels of 12.8 and 25.7 lM/l, intra-assay coefficients of variation (CV) were 1.3% and 2.1%, respectively. (alzrisk.org)
  • At mean levels of 34.5 and 98.8 pM/l intra-assay total CV were 6.6% and 6.0%, respectively. (alzrisk.org)
Principle1
Quantitative3
  • An immunoassay was used for quantitative analysis of MTX levels. (lww.com)
  • There are three assay platforms for SH2 profiling: quantitative far-Western, rosette, and oligonucleotide-tagged multiplex (OTM) assays [ 10 ]. (biomedcentral.com)
  • Speed and accuracy are key when analyzing nucleic acids or proteins, as is the ability to accommodate a wide variety of quantitative DNA, RNA and protein assays, and ELISAs. (tecan.com)
Quantification1
  • It enables researchers to further increase their throughput for IgG quantification, with the ability to analyze up to 4X the number of samples per plate, using 33% less sample volume, versus the traditional Valita Titer 96-well assay. (mybeckman.ca)
Analyzer2
  • Plasma total homocysteine concentrations are calculated by the Abbott AxSym Immunoassay Analyzer using a machine-stored calibration curve. (cdc.gov)
  • EDTA plasma samples were analyzed for total homocysteine (tHcy) with the Abbott IMx analyzer at the Laboratory of Clinical Chemistry of the VUMC. (lasa-vu.nl)
Homocysteine2
Ligand12
  • A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
  • There are numerous types of ligand binding assays, both radioactive and non-radioactive. (wikipedia.org)
  • As such, ligand binding assays are a superset of radiobinding assays, which are the conceptual inverse of radioimmunoassays (RIA). (wikipedia.org)
  • Some newer types are called "mix-and-measure" assays because they do not require separation of bound from unbound ligand. (wikipedia.org)
  • Ligand binding assays are used primarily in pharmacology for various demands. (wikipedia.org)
  • Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
  • The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
  • The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
  • The first successful ligand binding assay was reported in 1960 by Rosalyn Sussman Yalow and Solomon Berson. (wikipedia.org)
  • As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
  • Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
  • In this assay, target cells are loaded with fluorescence enhancing ligand, BATDA, which penetrates the cell membrane quickly and is hydrolyzed to form a hydrophilic ligand (TDA) that is released from cells only after cytolysis (Figure 1). (bmglabtech.com)
Transwell2
  • Thus, the aim of this study was to evaluate the ability of A. butzleri to survive as a long-term endosymbiont of A. castellanii for 30 days in two models of symbiotic interaction with A. castellanii: (i) endosymbiotic culture followed by gentamicin protection assay and (ii) transwell co-culture assay. (bvsalud.org)
  • Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
Luminescence2
  • Serum levels of vitamin B12 were determined at the Endocrine Laboratory of the VUMC with a competitive immunoassay luminescence on the automated ACS 180 System (Bayer Diagnostics, Mijdrecht, The Netherlands). (lasa-vu.nl)
  • Digital dispensing and luminescence-based assays play an important role in the evaluation of viral growth in different experimental models. (tecan.com)
20201
  • 2020. Inhibition of Mitochondrial Permeability Transition Pore Evaluated by Calcium Retention Capacity Assay . (news-medical.net)
ELISA1
  • Enzyme-linked immunosorbent assay (ELISA) was for IL-6 levels in peritoneal fluid and cell culture supernatant. (endometriosi.it)
Measurement1
  • We aimed to validate an immunoassay for the measurement of MTX-PG in erythrocytes. (eur.nl)
Kinetic experiments1
  • In this blog, we explore what it takes to tame automated cell imaging assays, and take back control of kinetic experiments to get reliable results more quickly, with fewer errors and less aggravation. (tecan.com)
Protein-protein int1
  • Figure 1 depicts the expected assay results for a 1:1 protein-protein interaction. (bmglabtech.com)
Vitro2
  • 6859 Quality Way Portage, MI 49002 Eurofins AvTech Labs offers a range of analytical and bioanalytical services including in vitro ADME assays, in vivo PK, bioanalysis, immunoassay development and biomarker ID and GMP method development. (contractresearchmap.com)
  • We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
Interactions2
  • We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
  • The assays rely on interactions between a fluorescently labeled IgG Fc-specific probe and the Fc of an IgG. (mybeckman.ca)
Radioactive1
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
Determination2
Quantitate1
  • We build robust immunoassay and enzyme activity kits to quantitate biomolecules in biological matrices, provide custom assay testing services, and offer world-class customer support. (contractresearchmap.com)
Antibodies1
  • The SH2-PLA assay utilizes oligonucleotide-conjugated anti-GST and anti-EGFR antibodies recognizing a GST-SH2 probe and cellular EGFR, respectively. (biomedcentral.com)
Phosphorylation2
  • We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
  • In-Cell Western assay was used for protein expression of CCL17 and phosphorylation levels of ERK, JNK, and P38. (endometriosi.it)
Analyze1
  • Landsteiner's work demonstrated that immunoassay technology allowed researchers to analyze at the molecular level. (wikipedia.org)
Membrane3
  • After opening of MPTP, fluorescence of membrane-impermeable CalciumGreen™ dye is enhanced as Ca 2+ leaches into the buffer. (news-medical.net)
  • These assays focus on changes that occur during apoptosis - the annexin V and propidium iodide assays are based on changes in membrane properties and permeability, and the TUNEL assay is based on apoptosis-related DNA fragmentation. (bmglabtech.com)
  • Assaying apoptosis in mixed cell populations usually requires the cells of interest to be pre-loaded with a reagent, and the loss of membrane integrity that occurs during cell death results in the reagent being released. (bmglabtech.com)
Proteins1
  • One limitation of current SH2 profiling methods is that these assays themselves do not provide the identity of the binding proteins. (biomedcentral.com)
Cells3
  • These and other assays are extremely good in evaluating apoptosis in a homogenous cell population, but are less useful when evaluating apoptosis in only one type of cell when a mixture of cells is present. (bmglabtech.com)
  • The most widely used assay is to pre-load cells with radio-labeled chromium. (bmglabtech.com)
  • The effects of AKBA on the cell viability in A549, H460, H1299, and BEAS-2B cells were determined by the CCK-8 assay. (boswellia.org)
RESULTS2
  • It is shown that this assay produces results that are similar to the classical chromium release assay. (bmglabtech.com)
  • The need for more physiologically relevant and timely results from cell-based assays continues to be a major challenge. (tecan.com)
Recognition1
  • The device has also shown a reliable fluorescent recognition of the assay. (news-medical.net)
Extensively1
  • Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. (lookformedical.com)
METHODS1
  • MATERIALS AND METHODS: A total of 202 hospitalized patients (71 with obesity and 131 without obesity) with a diagnosis of COVID-19 were included in the study. (bvsalud.org)
Method2
  • The method was compared with our in-house liquid chromatography tandem mass spectrometry (LC-MS/MS) method for total MTX-PG. (eur.nl)
  • A key factor of the Job plot also called the continuous variation method, is that the total concentration of the 2 molecules is held constant 2 . (bmglabtech.com)
Demands1
  • But behind every great imaging assay, pity the poor scientist grappling with the demands of biological variability and complex kinetic assays. (tecan.com)
Cell4
  • Several assays are available to monitor apoptosis and other forms of cell death. (bmglabtech.com)
  • The cell mediated cytotoxicity assay was carried out following the manufacturer's protocol with a few modifications. (bmglabtech.com)
  • The colony formation assay was used to identify the effects of AKBA on cell proliferation. (boswellia.org)
  • We therefore investigated whether oleandrin or a N. oleander extract could inhibit virus transmission from a GFP-expressing HTLV-1+ lymphoma T-cell-line to huPBMCs in co-culture assays. (longdom.org)
Device1
  • This generic type of device includes immunoassays and chromatographic assays for tacrolimus. (medicregister.com)
Plate2
  • Valita Titer plate-based, 96- and 384-well assays that offers a rapid, cost effective way to measure IgG. (mybeckman.se)
  • Valita Titer is among the industry's fastest IgG tests and is compatible with all plate readers with a Fluorescence Polarization module. (mybeckman.se)
Concentration1
  • Docetaxel area under the curve (AUC) and maximum concentration (Cmax) in each cycle and total cumulative AUC (AUCcum) were calculated and related to the incidence of adverse effects and tumour recurrence. (unav.edu)
Molecules1
  • The rotation of the molecules between absorption and emission of the photon has the effect of "twisting" the polarization of the light. (mybeckman.ca)
Levels1
  • Non-fasting serum lipid levels were measured using the Cobas Integra 400 Plus Chemistry analyser, an automated analyser that employs four different technologies: absorption photometry, fluorescence polarization immunoassay, immune-turbidimetry, and potentiometry for accurate analysis. (pgscatalog.org)
Development1
  • Through the development of RIA technology, researchers have been able to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. (wikipedia.org)
Https1
  • Retrieved on December 11, 2023 from https://www.news-medical.net/whitepaper/20180614/Inhibition-of-Mitochondrial-Permeability-Transition-Pore-Evaluated-by-Calcium-Retention-Capacity-Assay.aspx. (news-medical.net)
Provide1
  • Although binding assays are simple, they fail to provide information on whether or not the compound being tested affects the target's function. (wikipedia.org)
Competitive1
  • In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8 ]. (biomedcentral.com)
Format1
  • The 384-well format, alongside the 'add-mix-read' nature of the assay, with no requirement for sample pre-preparation or wash steps, makes it ideal for incorporation into a partially or fully automated bioprocess workflow. (mybeckman.ca)
Target1
  • Conclusion and Implications: These data suggest that LMT503 is a therapeutic candidate that can target macrophages to drive polarization with an immunosuppressive character and ameliorate IBD. (21docs.com)
Drugs1
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
Range1
  • The Valita Titer 384-well assay represents an expansion of ValitaCell's pioneering Quantum technology platform and the Valita Titer product range. (mybeckman.ca)
Therapeutic1
  • Experimental Approach: Here we show an IBD therapeutic candidate LMT503, a substrate which modulates NADH quinone oxidoreductase 1, which enhances NAD+ and induce anti-inflammatory macrophage polarization. (21docs.com)
Alternative1
  • We have developed PLA-SH2, an alternative in-solution modular domain binding assay that takes advantage of Proximity Ligation Assay and real-time PCR. (biomedcentral.com)
Obvious1