• Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
  • Thanks to recent developments in biotechnology, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. (routledge.com)
  • A transplastomic plant is a genetically modified plant in which genes are inactivated, modified or new foreign genes are inserted into the DNA of plastids like the chloroplast instead of nuclear DNA. (wikipedia.org)
  • Plants respond to various stimuli under abiotic or biotic stress condition and express certain genes either structural or regulatory genes which maintain the plant integrity. (scielo.br)
  • Transgenic constructs contain new combinations of genes that have never existed, and they also amplify gene products that have never been part of our food chain [3]. (i-sis.org.uk)
  • The expression of genes encoded within the T-DNA promotes the development and production of roots at the site of infection on most dicotyledonous plants. (bioone.org)
  • Inhibition of the Adp-Glucose Pyrophosphorylase in Transgenic Potatoes Leads to Sugar-Storing Tubers and Influences Tuber Formation and Expression of Tuber Storage Protein Genes. (mpg.de)
  • Thus, stress response studies during these growth stages reveal novel differentially regulated genes or proteins with important functions in plant stress adaptation. (intechopen.com)
  • GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware, and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. (nih.gov)
  • Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
  • Transgenic plants and animals or genetically modified organisms (GMOs) that lack federal approval for release. (tamuk.edu)
  • Those following the plant breeding and genetics stream can expect courses on plant genomics, tissue culture, transgenic technology and all the business aspects of the biotechnology industry. (chemistryworld.com)
  • Inhibition of Flower Formation by Antisense Repression of Mitochondrial Citrate Synthase in Transgenic Potato Plants Leads to a Specific Disintegration of the Ovary Tissues of Flowers. (mpg.de)
  • Leaf-Specific Antisense Inhibition of Starch Biosynthesis in Transgenic Potato Plants Leads to an Increase in Photoassimilate Export from Source Leaves During the Light Period. (mpg.de)
  • This technology has made the scientific community aware of the critical role of transgenic, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. (routledge.com)
  • The costs of meeting regulatory requirements and market restrictions guided by regulatory criteria are substantial impediments to the commercialization of transgenic crops. (nature.com)
  • Long-accepted plant breeding methods for incorporating new diversity into crop varieties, experience from two decades of research on and commercialization of transgenic crops, and expanding knowledge of plant genome structure and dynamics all indicate that if a gene or trait is safe, the genetic engineering process itself presents little potential for unexpected consequences that would not be identified or eliminated in the variety development process before commercialization. (nature.com)
  • Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind's ability to deliver precise genotypes in crops. (frontiersin.org)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • Humankind's radically increased ability to deliver precise genotypes in crops thanks to advances in breeding, transformation, transgenics, and editing is helping growers keep pace with increased demand for food and energy. (frontiersin.org)
  • In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
  • I have been drawing our regulators' attention to it at least since 1996 [1], when there was already sufficient evidence to suggest that transgenic DNA in GM crops and products can spread by being taken up directly by viruses and bacteria as well as plant and animals cells. (i-sis.org.uk)
  • Plant geneticists who have incorporated the promoter into practically all GM crops now grown commercially are apparently unaware of this crucial information [5]. (i-sis.org.uk)
  • With its work, the Leibniz Institute of Vegetable and Ornamental Crops (IGZ) contributes to a better understanding of plant systems and thus to the development of sustainable and resilient horticulture. (leibniz-gemeinschaft.de)
  • Several modifications of this editing system have been established for adoption in a variety of plants, as well as for its improved efficiency and portability, bringing new opportunities for the development of transgene-free improved varieties of economically important crops. (mdpi.com)
  • Selected candidates will perform gene and promoter isolation, vector constructions, tissue culture, transformation, and molecular analyses of transgenic crops. (bio.net)
  • Interest in duckweed has steadily regained momentum over the past decade, driven in part by the growing need to identify alternative plants from traditional agricultural crops that can help tackle urgent societal challenges, such as climate change and rapid population expansion. (osti.gov)
  • It has also been shown that the kn1 mRNA can be transported bidirectionally via phloem cells in plants (Duan et al. (springer.com)
  • The most common method for plastid transformations is biolistics: Small gold or tungsten particles are coated with the plasmid vector and shot into young plant cells or plant embryos, penetrating multiple cell layers and into the plastid. (wikipedia.org)
  • This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)
  • Transgenic DNA inserting into human cells, triggering cancer. (i-sis.org.uk)
  • The transgenic constructs used in genetic modification are basically the same whether it is of human cells or of other animals and plants. (i-sis.org.uk)
  • An aggressive promoter from a virus is often used to boost the expression of the transgene, in animal and human cells, from the cytomegalovirus that infects mammalian cells, and in plants, the 35S promoter from the cauliflower mosaic virus (CaMV) that infects Cruciferae plants. (i-sis.org.uk)
  • Unfortunately, although the CaMV virus is specific for plants, its 35S promoter is active in species across the living world, human cells included, as we discovered in the scientific literature dating back to 1989. (i-sis.org.uk)
  • In plants, several methods have been established for transcriptional analysis in tissue sections, cell types, and/or single cells. (portlandpress.com)
  • Here, we briefly review the evolution of spatial transcriptomics methods and we highlight recent advances and current challenges in sequencing, imaging, and computational aspects toward achieving 3D spatial transcriptomics of plant tissues with a resolution approaching single cells. (portlandpress.com)
  • Immediately after harvest, plant cells are very active such that they are vulnerable to adverse environmental conditions. (scialert.net)
  • Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
  • and the use of any human cells, tissues or body fluids. (scranton.edu)
  • Plant tissue culture includes techniques to propagate plants via somatic cells by using small parts called as explant on artificial growth mediums under sterile conditions. (intechopen.com)
  • This bacterium induces the neoplastic growth of plant cells that differentiate to form "hairy roots. (bioone.org)
  • Future research programs can be focused on the development of transgenic plants with enhanced stress tolerance in field conditions based upon the outcome of genomic approaches and knowing the mystery of nucleotides sequences hidden in cells. (intechopen.com)
  • The study confirms that the wide range of undesirable and unintended on-target and off-target mutations seen in gene-edited animal and human cells (see examples here ) also occur in plants. (gmwatch.org)
  • The foreign DNA in this case consisted of elements from the genetic construct - the plasmid - that was used to carry the gene-editing tool into the plant cells. (gmwatch.org)
  • Ferrier Lab fungi, botanicals, tissues and biofluids are prioritized by community-led meta-analytical consensus values from Project #1. (dal.ca)
  • 2014). With the help of several collaborators, we are evaluating potential impacts of transgenic chestnut on soil fungi, aquatic insects, terrestrial insects, other wildlife, persistence in the environment, tree growth rates, pollen flow, and surrounding plant communities. (esf.edu)
  • These results provide further evidence of induction of immune responses to fungi based on their ability to invade host tissue. (cdc.gov)
  • Filamentous fungi are ubiquitous microorganisms in indoor and outdoor environments and acquire nutrients from a wide variety of substrates such as decaying plant matter or water-damaged building materials [1] . (cdc.gov)
  • I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
  • In higher plants, FAD2 gene encodes the microsomal oleate Δ12-desaturase, one of the key enzymes essential for biosynthesis of polyunsaturated lipids that serve many important functions in plant development and stress responses. (researchsquare.com)
  • Interestingly, manipulation of carotenoid biosynthesis can also influence plant architecture, and positively impact growth and yield, making it an important target for crop improvements beyond biofortification. (frontiersin.org)
  • Here, we briefly describe carotenoid biosynthesis and highlight the latest advances and discoveries related to synthetic carotenoid metabolism in plants and microorganisms. (frontiersin.org)
  • The allocation of sugars from photosynthetic leaves to storage tissues in seeds, fruits, and tubers is an important determinant of crop yields. (biorxiv.org)
  • Synthesis of fructans in tubers of transgenic starch-deficient potato plants does not result in an increased allocation of carbohydrates. (mpg.de)
  • High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
  • The recent controversy over the transgenic contamination of the Mexican landraces [10] hinges on observations suggesting that the transgenic DNA with the CaMV 35S promoter is "fragmenting and promiscuously scattering throughout the genome" of the landraces, observations that would be consistent with our expectations [11]. (i-sis.org.uk)
  • and for revealing potentially novel strategies in plant defense and genome maintenance. (osti.gov)
  • Chloroplasts in plants are thought to have originated from an engulfing event of a photosynthetic bacteria (cyanobacterial ancestor) by a eukaryote. (wikipedia.org)
  • Similarly, I was not surprised by the research results released earlier this year by the Food Standards Agency [12], indicating that transgenic DNA from GM soya flour, eaten in a single hamburger and milk shake meal, was found transferred to the bacteria in the gut contents from the colostomy bags of human volunteers. (i-sis.org.uk)
  • Carotenoids are well-known isoprenoid pigments naturally produced by plants, algae, photosynthetic bacteria as well as by several heterotrophic microorganisms. (frontiersin.org)
  • The researchers also measured the growth performance of the transgenic lines and proved that MtPHY1 driven by the MtPT1 promoter is the best gene-promoted combination. (isaaa.org)
  • These early trees contain the wheat oxalate oxidase gene controlled by a vascular promoter, meaning the enzyme is predominantly present in the stem tissues where infections occur. (esf.edu)
  • A Truncated Version of an Adp-Glucose Pyrophosphorylase Promoter from Potato Specifies Guard Cell-Selective Expression in Transgenic Plants. (mpg.de)
  • Surprisingly, all promoter/cDNA combinations produced transgenic plants with red pericarp and red cob (RR pattern), indicating that the P1-wr promoter and encoded protein can function in pericarp. (iastate.edu)
  • Three tissue types were examined (leaves, hypocotyls and roots) and compared to analyses conducted with whole plants. (nih.gov)
  • Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis, however, closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment. (nih.gov)
  • Progress has been made to better understand about effect of regulons (AREB/ABF, DREB, MYB, and NAC) under abiotic stresses and a number of regulons reported for stress responsive and used as a better transgenic tool of Arabidopsis and Rice. (scielo.br)
  • FAD2 was first identified in the model plant species Arabidopsis thaliana [2]. (researchsquare.com)
  • Analysis of phenylpropanoid metabolites in transgenic tobacco showed that lignin content was affected when PAL activity is reduced to 20-25% of wild type levels (Bate et al . (scialert.net)
  • As a result, hairy roots are widely used as a trans-genic tool for the production of metabolites and for the study of gene function in plants. (bioone.org)
  • Diverse specialised metabolites contributed to the success of vascular plants in colonising most terrestrial habitats. (bvsalud.org)
  • Nevertheless, much progress has been made into plant transplastomics, for example, the production of edible vaccines for Tetanus by using a transplastomic tobacco plant. (wikipedia.org)
  • HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
  • Transgenic N. benthamiana plants expressing the CP gene of WMV II or ZYMV showed protection against symptom development when inoculated with WMV II and six other potyviruses: bean yellow mosaic (BYMV), potato Y (PVY), pea mosaic (PeaMV), clover yellow vein (CYVV), pepper mottle (PeMV), and tobacco etch (TEV). (apsnet.org)
  • This is partly because environmental risk assessment for transgenic plants is new and partly because the social context in which regulatory decisions about transgenic organisms must now be made is dramatically different from the social context in which these agencies are accustomed to working. (nationalacademies.org)
  • The Animal and Plant Health Inspection Service (APHIS) began its involvement in the regulation of transgenic organisms in the mid-1980s. (nationalacademies.org)
  • Most multicellular organisms have a capacity to regenerate tissue after wounding. (biologists.com)
  • The function of these enzymes was characterised in single and double mutant maize plants. (researchgate.net)
  • As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knock-down maize plants. (researchgate.net)
  • The invention discloses a method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries, and belongs to the technical field of tissue culture fast propagation and cultivation. (google.com)
  • shaping tissue culture seedlings at an early stage. (google.com)
  • Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. (scielo.br)
  • Currently, the majority of transplastomic plants are a result of chloroplast manipulation due to poor expression in other plastids. (wikipedia.org)
  • Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
  • All transgenic lines with higher levels of gene expression showed improved growth. (isaaa.org)
  • Hence, this study was conducted to examine the changes in PAL activity and gene expression patterns in relation to texture in the edible branchlet tissues during early postharvest handling. (scialert.net)
  • Structure and expression of mitochondrial citrate synthases from higher plants. (mpg.de)
  • The first gene being tested in transgenic American chestnut encodes an enzyme called oxalate oxidase . (esf.edu)
  • The process can be less efficient overall, since a larger team can produce many plants from which to select the elite edited plant for commercialization. (frontiersin.org)
  • Transgenic techniques are discussed for developing resistance to newly emerging diseases, pests, nutrient- and water-use efficiency, root traits, and improved tolerance to increasing temperature. (routledge.com)
  • Tolerance and susceptibility for plant under stress condition are complex events in which stresses may affect the multiple stage of plant development. (scielo.br)
  • The oxalate oxidase gene is also being tested by several other researchers to enhance pathogen tolerance in plant systems such as peanut , soybean , sunflower , and oilseed rape and also to possibly increase salt tolerance in tomato . (esf.edu)
  • Although we cannot rule out the possible existence of tissue-specific regulatory elements within the p1 non-coding sequences or flanking regions, the data from transgenic and natural alleles suggest that the tissue-specific pigmentation pattern characteristic of the P1-wr phenotype is epigenetically controlled. (iastate.edu)
  • So, only a correspondingly tiny fraction of the actual transfers would ever be detected, especially given the well-known tendency of transgenic constructs to fragment and rearrange. (i-sis.org.uk)
  • Post-doc Plant Molecular Biology Kansas State University 2-3 Post-doc positions will be available this summer and fall to investigate topics in molecular biology and transformation of soybean and cereals. (bio.net)
  • Issues in the Regulation of Genetically Engineered Plants and Animals (Washington, DC, 2004). (nature.com)
  • Plants show stress-specific regulation of transcription that affects their transcriptome under stress conditions. (intechopen.com)
  • 2009). Future toxicological studies: Previously, there have not been no reports concerning the effects of exposure of DEHP to transgenic mice with hPPARalpha(TetOff) or hPPARalpha(PAC) , but Ito and Nakajima (2008) reported that at a relatively high dose of DEHP (5.0 mmol/kg for 2 weeks) PPARalpha was activated in the liver of both genotyped mice. (cdc.gov)
  • Analysis of tissue sections indicated the presence of germinating spores in the lungs of mice challenged with A. fumigatus , but not A. versicolor . (cdc.gov)
  • One major limitation associated with existing protocols when used on economically important citrus species is likely low plant regeneration frequencies. (springer.com)
  • 2004). Moreover, PAL activity was found to be enhanced in several plant species in response to wounding imposed by either harvest or other stresses during postharvest handling as observed in newly harvested asparagus spears (Bhowmik et al . (scialert.net)
  • Complementary DNAs encoding eukaryotic-type cytidine-5'-diphosphate-diacylglycerol synthases of two plant species. (mpg.de)
  • The evolution of angiosperm plants between 50 and 200 million years ago resulted in the development of many antioxidant pigments - particularly during the Jurassic period - as chemical defences against reactive oxygen species that are byproducts of photosynthesis . (wikipedia.org)
  • Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues. (springer.com)
  • In plants, they are synthesized in plastids where they play essential roles in light-harvesting and in protecting the photosynthetic apparatus from reactive oxygen species (ROS). (frontiersin.org)
  • Plant species show large variation in the composition and content of their tannins and other polyphenols. (bvsalud.org)
  • Here, we used an automated group-specific UHPLC-DAD-MS/MS tool to detect and quantify eight most common polyphenol groups in 31 chemically diverse plant species representing many types of growth forms and evolutionary ages. (bvsalud.org)
  • Ten replicate plants were used for each species and two polyphenol-related bioactivities, i.e., protein precipitation capacity and oxidative activity were measured in all samples as well. (bvsalud.org)
  • lymphoid tissue, and digestive tract), which the animal model captures the It can be difficult to parse out concordance has often been ob- range of potential human response reasons for lack of tumour site con- served among different species after to the particular agent tested. (who.int)
  • Approaches to Influence Partitioning and Metabolism of Carbohydrates in Transgenic Plants. (mpg.de)
  • Antisense inhibition of cytosolic NADP-dependent isocitrate dehydrogenase in transgenic potato plants. (mpg.de)
  • The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
  • This disease is currently the biggest cassava constraint in East Africa, as it destroys the edible roots of the cassava even when rest of the plant looks healthy. (allianceforscience.org)
  • Plant biotechnology has now become an integral part of tissue culture research. (routledge.com)
  • The book traces the roots of plant biotechnology from the basic sciences to current applications in the biological and agricultural sciences, industry, and medicine. (routledge.com)
  • Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research, and industry and also of interest to policy makers, social activists and regulatory and funding agencies. (routledge.com)
  • Abiotic stress is the key cause of crop hammering globally, reducing average yields of most of the major crop plants. (scielo.br)
  • To apply please send c.v. and three letters of recommendation to: Dr. Harold N. Trick, Department of Plant Pathology, Kansas State University, Manhattan Kansas, 66506-5502. (bio.net)
  • Shoots and roots are regenerated from explants, and consequently, the whole fertile plants are reconstituted under certain cultural conditions. (intechopen.com)
  • Hairy roots are induced by the incorporation of a bacterial-derived segment of DNA transferred (T-DNA) into the chromosome of the plant cell. (bioone.org)
  • In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)
  • We had orange roots," said Dr. Nigel Taylor, director of the Institute for International Crop Improvement at the Donald Danforth Plant Science Center, in an interview with the Alliance. (allianceforscience.org)
  • It describes the recent biotechnological advancement and developments in plant tissue culture and transgenic. (routledge.com)
  • Plant tissue culture techniques such as such as micropropagation, regeneration, somaclonal variation, somatic embryogenesis, anther/pollen/embryo culture are discussed for genetic improvement of crop plant. (routledge.com)
  • Plant cell has the ability of forming whole fertile plant which is called 'totipotency', under in vitro culture conditions. (intechopen.com)
  • High-frequency shoot regeneration is one of the main aims of in vitro culture and it is a prerequisite to guarantee the success in transformation studies and in clonal propagation of plants. (intechopen.com)
  • In this chapter, the importance of tissue water content on in vitro culture response is discussed. (intechopen.com)
  • Plant tissue culture belongs to totipotency meaning that a whole plant can be reproduced from a single cell in growth medium. (intechopen.com)
  • The process of tissue culture caused them to lose resistance to CMD… but transgenic CBSD resistance remained," explained Dr. Andrew Kiggundu, project manager of the Institute for International Crop Improvement at the Donald Danforth Plant Science Center at St. Louis, Missouri, in an interview with the Alliance for Science. (allianceforscience.org)
  • The current progression in tissue culture techniques, specifically micropropagation led to a new method of propagation through in vitro micropropagation medium used for propagation of potato is termed as potato micropropagation medium (PMM). (slideserve.com)
  • Plant/ crop productivity effected by the environmental strains such as water deficit condition (drought), Low temperature (cold), salt and High temperature (heat) these stresses disturb the signal transduction of gene regulatory systems of plants. (scielo.br)
  • As indicated in Chapter 1 , regulatory agencies charged with assessing the safety of transgenic plants face a daunting task. (nationalacademies.org)
  • APHIS's regulatory process has never led to the release of a transgenic plant that clearly caused environmental damage. (nationalacademies.org)
  • Plants are being sessile in nature it can't move from one place to other places and contentiously exposed by extensive array of environmental stresses like as water deficit condition (drought), low temperature (cold), salt and high temperature (heat) etc. (scielo.br)
  • Being sessile in nature, plants have developed different strategies to adapt and grow under rapidly changing environments. (intechopen.com)
  • Furthermore, development of a notification process that focuses on plant ecology was an important step in effectively streamlining the field-testing process. (nationalacademies.org)
  • Given this and other reports, it appears that transgenic plants that express a potyvirus CP gene will show at least a noticeable level of protection against symptom development when challenged by other potyviruses. (apsnet.org)
  • Our work demonstrated the importance of C18:2 and/or C18:3 in development of pollen exine and anther cuticle in cotton, and provided clue for further investigation of the physiological significance of the fatty acid composition in plant growth and development. (researchsquare.com)
  • This review details the anatomy, development, physiology, and molecular characteristics of the Lemnaceae to introduce them to the broader plant research community. (osti.gov)
  • Post-translationally modified peptides are involved in many aspects of plant growth and development. (elifesciences.org)
  • 2004), a mortality study of U.S. workers in a plastics manufacturing and research and development plant (Selenskas et al. (cdc.gov)
  • Neuroscience focusing on human development and disease has long been hampered due to ethical rea¬sons, low tissue availability, and low translatability from animal models. (lu.se)
  • Lignin is an essential polymer in vascular plants that plays key structural roles in vessels and fibers. (researchgate.net)
  • The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets. (scialert.net)
  • Textural changes or toughening of plant tissues is closely associated with lignin formation. (scialert.net)
  • Lignin, a polymer of aromatic subunits derived from phenylalanine serves as a matrix around the polysaccharide components of some plant cell walls, providing rigidity and comprehensive strength (Whetten and Sederoff, 1995). (scialert.net)
  • However, tissues with lack of water are usually not successful in regenerating a satisfactory amount of shoots. (intechopen.com)
  • While no effective HLB control methods or HLB resistance citrus cultivars have been commercialized, transgenic plant technologies have been recognized as a powerful tool to control the HLB disease. (springer.com)
  • An efficient genetic transformation protocol is a key step for using transgenic technologies to improve citrus quality, productivity, and resistance to abiotic and biotic stresses such as HLB. (springer.com)
  • While toughness of the tissue or breaking force as estimated by resistance to pressure on shearing continuously increased, PAL activity decreased after few hours from harvest. (scialert.net)
  • Genetically guided selection and transgenic modification of plant membrane transporters can help enhance crop yields and increase pathogen resistance. (biorxiv.org)
  • The mission is to provide resistance to both diseases… So they are doing field work to back cross the CMD resistance… Now there are about 300 lines that are transgenic and have both CMD and CSBD resistance… and they are crossing it back to non-transgenic, which is CMD resistant… Now they have the first generation," he said. (allianceforscience.org)
  • It is almost exclusively of exogenous origin and is delivered mainly from dietary plant chlorophyll and, to a lesser extent, from animal sources. (medscape.com)
  • Regeneration of a patterned multicellular organism from isolated pieces of adult somatic tissues is a remarkable phenomenon that occurs both in plants and animals ( Morgan, 1901 ). (biologists.com)
  • Infectious agents isolated from plants or animals in nature. (tamuk.edu)
  • Creating transgenic animals and plants. (harvard.edu)
  • [ 6 , 7 ] Medawar's postgraduate student, now envisioned the possibility of having adult animals accept tissue allografts by reproducing in the laboratory what occurred naturally in cattle. (medscape.com)
  • On the other hand, transgenic plants produced from mature tissue via genetic transformation can drastically shorten flowering and fruiting time and thus speed up field evaluation studies. (springer.com)
  • Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. (scielo.br)
  • Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. (biologists.com)
  • Micropropagation and induction of autotetraploid plants of Chrysanthemum cinerariifolium (Trev. (bioone.org)
  • The gradual induction of defenses in stressed plant populations could be monitored on a yearly basis unless a seasonal and yearly variation in natural defense levels obscures such monitoring schemes. (bvsalud.org)
  • Whilst the transformation efficiency is lower than in agrobacterial mediated transformation, which is also common in plant genetic engineering, particle bombardment is especially suitable for chloroplast transformation. (wikipedia.org)
  • Other transformation methods include the use of polyethylene glycol (PEG)- mediated transformation, which involves the removal of the plant cell wall in order to expose the "naked" plant cell to the foreign genetic material for transformation in the presence of PEG. (wikipedia.org)
  • PEG- mediated transformation however, is notoriously time consuming, very technical and labor intensive as it requires the removal of the cell wall which is a key protective structural component of the plant cell. (wikipedia.org)
  • Whilst no study has been attempted yet for plastid transformation of higher plants using electroporation, this could be an interesting area of study for the future. (wikipedia.org)
  • They used a stable transformation method that ensured that the CRISPR editing tool remained active in the plants over four generations, so that they could examine the effects over time. (gmwatch.org)
  • In higher plants, the microsomal oleate Δ12 -desaturase (fatty acid desaturase 2, FAD2) is a hydrophobic endoplasmic reticulum protein, catalyzing the reaction from monounsaturated oleic acid (C18:1) to polyunsaturated linoleic acid (C18:2) by introducing a double bond between the 12th and 13th carbon atoms of C18:1 [1]. (researchsquare.com)
  • I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)