Tissues during embryonic human cells. Medical search. Frequent questions

Directed differentiation of human embryonic stem cells (hESCs) has generated much interest in the field of regenerative medicine. (ca.gov)
Hence, regulation of selective MMPs and TIMPs occurs during differentiation of human neural precursors suggesting that MMP-2 and TIMP-4 in particular may perform regulatory roles in the developing CNS. (uea.ac.uk)
FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast. (mpg.de)
Our current focus is to learn how to direct and efficiently drive controlled differentiation of human stem cells into subtype-specific neurons. (lu.se)

Spatiotemporal analysis of stem-cell-derived embryos offers conceptual and technical advances in the field. (newswise.com)
For instance, Science University and Oregon Health are engaged in developing blastocyst staged embryos of around 150 cells in order to provide hESC source. (grandviewresearch.com)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
1] Therapeutic cloning, on the other hand, creates human embryos merely as a source of embryonic stem cells. (reasons.org)
Tragically, however, in order to harvest stem cells from human embryos, the embryos must be destroyed. (reasons.org)
Crudely put, therapeutic cloning looks to generate human embryos solely for the body parts they can provide. (reasons.org)
Recent and ongoing research suggests an alternative approach that can achieve the same goal (repair of damaged or diseased organs) without destroying human embryos. (reasons.org)
This means that hundreds of human embryos would die to achieve a single live human clone birth. (reasons.org)
They took other mouse embryos and put in the human version. (upr.org)
These cells, taken from days' old embryos called blastocysts, have the ability to form any tissue in the body. (harvard.edu)
Mouse embryos exposed to valproic acid in the womb develop similar birth defects to human embryos. (livescience.com)
In human embryos, that's typically between the fourth and sixth week of pregnancy, according to the Centers for Disease Control and Prevention (CDC). (livescience.com)
The researchers exposed the organoids to valproic acid and found that the drug pushed the organoids' neuroepithelial cells into senescence, just as it had in the mouse embryos. (livescience.com)
Scientists announced this week that they have successfully produced embryonic stem cells by transferring the DNA of human skin cells into unfertilized human eggs to produce embryos, a technique the Church considers to be an abuse of human life. (womenofgrace.com)
An Indiana woman's brain tumor turned out to contain hair, bone and teeth, and has been dubbed her "embryonic twin" - but experts say that such tumors are not actually twins, nor are they embryos. (livescience.com)
Although these tumors can originate during embryonic development, they aren't embryos, and they are not a person's "twin. (livescience.com)
Chick embryos are easily accessible, and their nervous system development is similar to that of human embryos at comparable stages. (lu.se)
To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)

In the ongoing debate over embryonic stem cells some have argued that the cells have not demonstrated their potential value in medicine. (news-medical.net)
During a recent appearance on the Oprah Winfrey show, the popular cardiovascular surgeon and television personality, Dr. Mehmet Oz, announced to a stunned audience that due to advances in adult stem cell technology, the debate over embryonic stem cells "is dead. (womenofgrace.com)

The use of gametes, embryonic and fetal tissue as well as blood and blood products raises additional questions that need to be separately addressed. (who.int)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

These are the pluripotent cells that possess the ability to get differentiated into all germ layers. (grandviewresearch.com)
This complex process occurs in several steps and controls the development of pluripotent cells into highly specialised cells of an organism. (europa.eu)

and transplantation of in vitro grown organs and tissues (tissue engineering). (wikipedia.org)
Nonetheless, the transplantation of organs and tissues does raise ethical concerns. (who.int)
Sex differences in the human body affect many different organs and tissues, some of them have an effect on the human brain and its development. (dissertations.se)
The damage caused by thalidomide was not mutually exclusive, with the majority of children exhibiting damage to multiple organs and tissues [ 3 , 7 ]. (hindawi.com)

We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
Accelerated uptake of ESC based cell therapy on in vivo and in vitro principle is expected to fuel growth through to 2024. (grandviewresearch.com)
When epithelial cells transform into mesenchyme in the embryo, or when they are induced to do this in vitro, they switch from the keratin intermediate filament profile to one rich in vimentin, and the effect of cell matrix interaction on cell shape is profoundly altered. (nih.gov)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
Recently evidence was provided that the integration of cell-free nucleic acid with host cells occurs in vivo as well as in vitro . (fightaging.org)
These observations will facilitate molecular characterization of PSC-derived RGCs during differentiation, critical knowledge for establishing the veracity of these in vitro produced cells. (caltech.edu)
Recombinant expression, in vitro refolding, and biophysical characterization of the human glucagon -like peptide-1 receptor. (ncbcs.org)
To allow a biophysical characterization in vitro, we expressed the human full-length GLP-1R within the cytosol of Escherichia coli as inclusion our bodies. (ncbcs.org)
We also develop technologies for direct conversion of human fibroblasts into functional and subtype-specific neurons in vitro , and the conversion of endogenous glia into neurons in vivo . (lu.se)
In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)
5. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. (bvsalud.org)
11. Kramer PR, Nares S, Kramer SF, Grogan D, Kaiser M. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. (bvsalud.org)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Wnt signaling is critical for normal embryonic development of the skin, bones, and other structures. (medscape.com)
Since Wnt signaling proteins cannot be released without the PORCN protein, and Wnt signaling is important for normal embryonic development, the defects found in this disorder are related to lack of Wnt signaling. (medscape.com)
We are intrigued by how we can utilize the setting of normal embryonic development to understand tumor initiation. (lu.se)

Transplantation of human organs and tissues1 saves many lives and restores essential functions in circumstances when no medical alternative of comparable effectiveness exists. (who.int)
The number of human tissue transplants is increasing in both developed and developing countries, but global data on this form of transplantation are less complete. (who.int)
1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)
Successful transplantation of organs and living tissues depends on continued medical follow-up and the patient's compliance with a regimen of immunosuppressive drugs. (who.int)
Nevertheless, despite their immunogenicity in vivo, transplantation tolerance may be readily established by using minimal host conditioning with nondepleting monoclonal antibodies specific for the T cell coreceptors, CD4 and CD8. (ox.ac.uk)
One such approach, called "xenotransplantation" (the transplantation of living cells, tissues, and organs from one species to another species), turns to pigs as a source of organs for human transplants. (reasons.org)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
In co-culture with human cortical neurons and after transplantation (AT) into human brain tissue resected. (lu.se)
In co-culture with human cortical neurons and after transplantation (AT) into human brain tissue resected from patients with drug-resistant epilepsy, light-activated channelrhodopsin-2 (ChR2) expressing hdINs induced postsynaptic currents in human neurons, strongly suggesting functional efferent synapse formation. (lu.se)
This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)

This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)

These data establish that subpopulations of proliferating hESCs whose tissue fate is predetermined exist, and challenge the notion that all cells within proliferating hESC cultures are truly 'pluripotent. (ca.gov)
This co-culture approach also will enable identification of other distinct hESC subpopulations, and selection for these should prove valuable in generating tissue-specific reagents for cell-based therapy. (ca.gov)
We generated light-sensitive human embryonic stem cell (hESC)-derived GABAergic interneurons (hdIN) and tested their functionality. (lu.se)
These results provide a proof-of-concept that hESC-derived neurons can integrate and modulate the activity of a human host neuronal network. (lu.se)
To characterize RGCs in human embryonic stem cell (hESC) derived retinal organoids we examined RGC markers and surface antigen expression and made comparisons to human fetal retina. (caltech.edu)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

The differentiated cells are further used for therapeutic applications in regenerative medicine. (grandviewresearch.com)
Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)
Although the natural privileged status of ES cell-derived tissues is, therefore, insufficient to overcome even mH barriers, our findings suggest it may be harnessed effectively for the induction of dominant tolerance with minimal therapeutic intervention. (ox.ac.uk)
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology examines recent progress in our understanding of stem cell biology and how the properties of stem cells can be manipulated for therapeutic purposes, emphasizing the roles that cutting-edge technologies and interdisciplinary approaches have played in this work. (cshlpress.com)
Hence, recapitulating the islet ECM in both isolated islets and stem-cell-derived β-cells is of high therapeutic value. (nature.com)
In this regard, cell-based therapy may be an alternative therapeutic approach. (lu.se)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
3. developed new embryonic stem cell-based approaches to screening of small candidate molecules for therapeutic applications in human diseases. (europa.eu)
Some of these retinal tissues such as Retinal Pigment Epithelium (RPE) may provide a novel therapeutic strategy for such devastating diseases as macular degeneration, retinitis pigmentosa, and other retinal degenerative diseases. (news-medical.net)
With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
Regardless of current curiosity within the therapeutic potential of recombinant human insulin-like development factor-I (rhIGF-I) within the remedy of diabetes mellitus, its mechanism of motion remains to be not outlined. (ncbcs.org)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

During embryonic development, the extracellular matrix (ECM) promotes the production of differentiated products by epithelial cells and the migration of mesenchymal cells, and probably also plays a role in epithelial-mesenchymal transformation. (nih.gov)
Here we examine the role of the cell skeleton (actin, microtubules, intermediate filaments) in mediating matrix effects on mesenchymal cell morphology, migration, and formation. (nih.gov)
The interaction of both epithelial cells and mesenchymal cells with ECM seems to involve the actin cortex, which is best developed in the base of the epithelial cell, where it attaches to underlying matrix via membrane-intercalated receptors. (nih.gov)
A new hypothesis states that the microtubule- and intermediate filament-rich endoplasm, containing the nucleus, moves past the actin cortex-receptor-matrix complex into the newly synthesized front end of the mesenchymal cell to effect forward movement. (nih.gov)
Vimentin-actin interactions with ECM may be a major factor in the ability of a cell to become mesenchymal. (nih.gov)
RepliCel Life Sciences Inc. today announced that it has been granted a patent by Japan's Ministry of International Trade and Industry for hair follicle mesenchymal stem cells and their use thereof. (baldingblog.com)
Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)

Scientists would like to understand what the genetic basis is for humans' apparently special capacity for logic, abstract thought, complex emotions and language. (upr.org)
In abstract, a framework was developed to supply useful human full-length GLP-1R by recombinant expression in E. coli as a prerequisite for eventual construction willpower and a rigorous biophysical characterization together with protein variants. (ncbcs.org)

Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)
The retinal progenitor cells (RPCs) of retinal organoids expressed CD184, consistent with its expression in the neuroblastic layer in fetal retina. (caltech.edu)
The focus of my research is to understand cell fate specification in the developing brain and in human neural progenitor cells using cell-based models of neuronal differentiation. (lu.se)
Although biochemical functions of the human PORCN gene are not well characterized, Wnt signaling may be involved in the phenotypic expression of focal dermal hypoplasia where defective/deficient Wnt signaling could affect cell fate or result in failure of a progenitor cell line to expand. (medscape.com)

Here, we defined the single-cell spatiotemporal gene expression landscape of human embryonic development with stem-cell-derived organoids. (newswise.com)
Overall, our study advances the spatiotemporal analysis of human embryonic development in stem-cell-derived organoids. (newswise.com)
Many chapters illustrate how single-cell profiling, stem-cell-derived organoids, intravital microscopy, lineage tracing, quantitative modeling, and other modern approaches have offered important insights. (cshlpress.com)
Human cortical organoids (hCOs), derived from human embryonic stem cells (hESCs), provide a platform to study human brain development and diseases in complex three-dimensional tissue. (nih.gov)
The study used mice and human organoids. (livescience.com)
To check if valproic acid could trigger senescence in human cells, the team ran a similar experiment using 3D clusters of human nerve cells, known as cerebral organoids. (livescience.com)
These organoids resemble miniature human brains , in that their structure and function is similar to that of the full-size organ. (livescience.com)

Stroke is a severe cerebrovascular disease in which the neuronal tissue and vasculature of the brain undergo ischemia-evoked alterations. (dissertations.se)
Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

Time-series single-cell RNA sequencing paired with single-cell resolution spatial revealed human embryonic development signatures such as extraembryonic placenta, yolk sac hematopoiesis neural crest, blood vessels, and cardiac mesoderm. (newswise.com)
Cell-cell communication network analysis demonstrated that trophoblast-like tissues supplied WNT signaling in neural crest cells to facilitate maturation and migration. (newswise.com)
What we discovered is that the human DNA turned on gene activity in neural stem cells, and these are cells which produce the neurons of our cerebral cortex," says Silver. (upr.org)
This upset seems to occur around the time that the "neural tube" - a hollow tube of tissue that later becomes the brain and spinal cord - forms and closes. (livescience.com)
By simultaneously knocking out Foxi3 in neural plate border cells and following their fates, we show that neural plate border cells lacking Foxi3 contribute to all four lineages of the ectoderm - placodes, epidermis, crest and neural tube. (bvsalud.org)
We contrast Foxi3 with another neural plate border transcription factor, Zic5, the progenitors of which initially contribute broadly to all germ layers until gastrulation and gradually become restricted to the neural crest lineage and dorsal neural tube cells. (bvsalud.org)
Analysis of neural, neural crest and placodal markers reveals that individual NPB cells co-express competing transcriptional programmes suggesting that their ultimate identify is not yet fixed. (bvsalud.org)
Considering our findings, we propose a probabilistic model for cell fate choice at the neural plate border. (bvsalud.org)
These cancers originate from cells of the sympathetic nervous system (SNS), in turn derived from the transient embryonic neural crest stem cell population. (lu.se)

TS-REX was applied to predict regulators of Polycomb group genes in six human tumor tissues and in human embryonic stem cells. (lu.se)

Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression. (mpg.de)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
But the molecular mechanism that explained this process, and how the cell could use it to respond to types of inflammation and injury that it had never seen before, remained a mystery. (scitechdaily.com)
This volume is therefore an indispensable reference for molecular, cell, and developmental biologists, as well as anyone wishing to explore the possibilities of stem cells in regenerative medicine and tissue engineering. (cshlpress.com)
Our team of scientists also investigates the molecular mechanisms that underlie embryonic development and tissue regeneration. (edu.au)
Methodologies include cell and molecular biology, biochemistry, and microscopy - combined with proteomics to examine cell physiology, inter and intracellular signalling, cell structure, and organelle function, both on a cellular and molecular level. (edu.au)
The specimens were examined by stan- causative agent of cat-scratch disease (CSD), has been dard, cell culture, and molecular methods. (cdc.gov)
These cells expressed RPE-specific molecular markers and were capable of phagocytosis, an important RPE function. (news-medical.net)
Differentiomics could also be a valuable predictive tool for quality assessment of other ES cell derivatives based on their molecular signature. (news-medical.net)

Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)

Haseltine was briefed on the project to isolate human embryonic stem cells and embryonic germ cells at Geron Corporation in collaboration with researchers at the University of Wisconsin-Madison and Johns Hopkins School of Medicine. (wikipedia.org)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)
The researchers then used these cells as the source of genetic material to clone pigs with organs that lacked the sugar groups responsible for HAR. (reasons.org)
Humans and chimpanzees have DNA that's remarkably similar - researchers say our genetic code is about 95 percent identical . (upr.org)
18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
The event was the first for the new institute, which will bring together about 100 researchers working on stem cells at Harvard schools and at seven teaching hospitals. (harvard.edu)
Institute planners expect to host a major symposium annually similar to Friday's as a way to stimulate the exchange of ideas and foster a sense of community among stem cell researchers at Harvard. (harvard.edu)
Stem cells have generated excitement among researchers from their ability to both regenerate themselves and to produce specialized tissue cells. (harvard.edu)
It is that potential that has researchers thinking the cells may hold the key to treating or curing diseases related to organ or issue failure, such as diabetes, Parkinson's, Alzheimer's, heart disease, spinal cord injury, and muscular dystrophy. (harvard.edu)
The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)

IHC-P: Human lung and HEK-293T (human embryonic kidney epithelial cell) transfected with a SCGB1A1 expression vector containing a his tag tissues. (abcam.com)

However, implementation remains a controversial topic due to diverse views in context to the moral and legal status of early embryo cell lines. (grandviewresearch.com)
Opponents argue that any embryo has the potential to develop into a mature human. (cbc.ca)
The contributors describe how the first cell lineages arise in the mammalian embryo, stem cell dynamics during the development and homeostasis of specific tissues (e.g., epithelia and brain), and what happens when stem cell integrity is compromised (e.g., by DNA mutations). (cshlpress.com)
The human version of a DNA sequence called HARE5 (inserted into this mouse embryo) turned on a gene that's important for brain development. (upr.org)
Doctors discovered she had a teratoma, a type of tumor that can contain all three of the major cell types that are found in an early stage human embryo. (livescience.com)
Because teratomas can have all three of the cell types found in the developing embryo, they can contain a variety of tissues, including cartilage, bone and hair, said Dr. Amir Dehdashti, director of cerebrovascular neurosurgery research at North Shore University Hospital in Manhasset, New York. (livescience.com)
Typically, during the development of a human embryo, germ cells migrate to the gonads. (livescience.com)

In contrast, CD135(+)GFP(+) hESCs gave rise to tissues representing all three embryonic germ layers, and were virtually indistinguishable from CD135(-)-derived tissues. (ca.gov)
Its major objective was to produce a gene expression atlas covering the development of ES cells into all three germ layers (ecto-, meso-, and endoderm) and into somatic cells. (europa.eu)

The expression of TIMP-1, TIMP-2 and TIMP-3 was unchanged following stem cells differentiation into neurons and glia. (uea.ac.uk)
In the developing nervous system, sex differences can bias the number or functionality of neurons, glial cells or synapses. (dissertations.se)
In addition to having bigger brains, Silver says, humans also "have more neurons, and we have more connections between these neurons. (upr.org)
In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
The use of stem cell-derived dopamine neurons or deep brain stimulation (DBS) represents two alternative approaches to treat Parkinson's Disease. (lu.se)

In Europe, hundreds of thousands of tissue transplants are performed each year, and in 1999 an estimated 750 000 people in the United States of America received human tissue, twice as many as in 1990. (who.int)
In fact, the research team oversaw the birth of four normal, healthy piglets with organs suitable for human transplants. (reasons.org)

L-15 supports established cell lines as well as primary explants of embryonic and adult human tissue. (sigmaaldrich.com)
Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)

Single-cell level characterization of embryonic development is a major benchmark of human developmental biology. (newswise.com)
TS-REX covers ESTs from H. sapiens and M. musculus, and allows the characterization of both presence and specificity of transcription factors in user-specified tissues or cell types. (lu.se)
Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)

These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)

The first cell therapies were intended to slow the aging process. (wikipedia.org)
Significant involvement of the Japanese government in the development of cell line based therapies is a significant factor and is expected to provide lucrative growth avenues. (grandviewresearch.com)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
In addition, the authors consider the potential of stem-cell-based therapies in the clinic (e.g., for treating retinal diseases and skin disorders) and the innovations that are facilitating the development of those therapies, including various lineage reprogramming strategies and new biomaterials that modulate stem cell properties. (cshlpress.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
This brings as oral dopamine therapies with the advantage that with it continued infective risks for the patients the cells can be targeted to the site where dopamine with DBS which is not so for patients with trans- is most needed, namely the striatum. (lu.se)

My PhD focussed on investigating the distributions and possible functions of nitric oxide/nitric oxide synthases in the physiology and pathology of a variety of tissues and organs and I was fortunate enough to publish some of the first papers on the involvement of these molecules in human diseases, notably in the cardiovascular system. (nottingham.ac.uk)
I cover mainly cell biology and physiology. (nottingham.ac.uk)
Pig organ size and physiology are comparable to that of humans. (reasons.org)

4. Nakashima M, Akamine A. The application of tissue engineering to regeneration of pulp and dentin in endodontics. (bvsalud.org)

When the cell source for a regenerated organ is derived from the patient's own tissue or cells, the challenge of organ transplant rejection via immunological mismatch is circumvented. (wikipedia.org)
Nor are they sure how well a patient's body will accept stem cell treatments derived from cells donated by a stranger, or whether the growth of implanted cells can be managed. (latimes.com)

Pinning proteins to its genetic material at the height of inflammation, the cells bookmark where they left off in their last tussle. (scitechdaily.com)
Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
To address this issue using a mouse model of CRT, we have derived a panel of ES cell lines that differ from CBA/Ca recipients at defined genetic loci. (ox.ac.uk)
We explored the use of naturally occurring somatic mutations in mitochondrial transcripts detected in single-cell RNA-seq as genetic lineage barcodes in HPSC. (biorxiv.org)
In this study, we used an enrichment of scRNA-seq mitochondrial reads and a robust computational method to identify clonally relevant mitochondrial variants as endogenous genetic barcodes for clonal tracking of early embryonic hematopoiesis from hPSC. (biorxiv.org)
Most of the genetic differences between humans and chimps are actually found in the so-called junk DNA, Pollard notes. (upr.org)
10 Oct, 2007 12:18 pm The field of functional genomics explores the various functions of genetic sequences within the human genome. (scitizen.com)
One question with high relevance to aging is whether or not cfDNA fragments can behave as mobile genetic elements, illegitimately integrating in the chromosomal DNA of healthy cells in its own host, thereby contributing to genome instability and possibly causing age-related functional decline and age-related pathophysiological processes. (fightaging.org)
Focal dermal hypoplasia (FDH) is an uncommon genetic disorder affecting tissues of ectodermal and mesodermal origin. (medscape.com)

Neurodegeneration and neurodegenerative diseases (including the shared mechanisms of nerve cell death that contribute to many diseases), Vascular Cognitive Impairment and Dementia (VCID), NINDS tissue/cell resources, basic invertebrate neuromuscular junction (NMJ). (nih.gov)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
This makes them perfect for a wide range of medical uses, from repairing tissue to treating diseases such as Parkinson's and Alzheimer's. (cbc.ca)
Given their plasticity and regenerative abilities, stem cells provide opportunities for treating human diseases such as diabetes. (cshlpress.com)
Implanting replacement tissue into damaged and diseased organs would provide the opportunity to treat and possibly cure many dreaded diseases and debilitating injuries. (reasons.org)
California voters received, instead, a TV campaign promising cures tomorrow for a host of diseases, some of which may never respond to stem cell therapy. (latimes.com)
All these diseases are characterised by the irreversible loss of functional cells. (europa.eu)
Harvard's ability is critical in this case, Summers said, given the promise of stem cells to cure organ or tissue failure from diseases that afflict 150 million nationally. (harvard.edu)
Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
Do you know you can use your own body cells to fight diseases? (medicaltourismco.com)
All these diseases are readily treated by Stem Cell Therapy in India at a reasonable cost. (medicaltourismco.com)
Symptoms and Signs Amyotrophic lateral sclerosis and other motor neuron diseases are characterized by steady, relentless, progressive degeneration of corticospinal tracts, anterior horn cells, bulbar motor nuclei. (msdmanuals.com)

But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
For instance, the medication changes the levels of certain chemical messengers in the brain and alters which genes can be switched on in a cell at any given time. (livescience.com)
However, when applying this concept to genomes of multicellular organisms such as mammals, one has to deal with a large number of false positive predictions since many transcription factor genes are only expressed in specific tissues or cell types. (lu.se)
We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)
The use of expression levels of transcription factor-encoding genes according to hierarchical anatomical classifications covering different tissues and cell types makes it possible to filter out irrelevant binding site predictions and to identify candidates of potential functional importance for further experimental testing. (lu.se)

The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)

Functional genomics of human pre-implantation development. (mpg.de)

By forcing key stem cells cells into this state, called senescence, valproic acid may disrupt brain development in the womb and therefore cause cognitive and developmental disorders down the line, according to the study, published Tuesday (June 14) in the journal PLOS Biology . (livescience.com)

This is happening in natural killer cells, T cells, dendritic cells from human skin, and epidermal stem cells in mice," says Samantha B. Larsen, a former graduate student in the laboratory of Elaine Fuchs at The Rockefeller University. (scitechdaily.com)
So the Fuchs lab once again exposed mice skin to irritants, and watched as stem cells in the skin changed. (scitechdaily.com)
Scientists studying the difference between human and chimpanzee DNA have found one stretch of human DNA that can make the brains of mice grow significantly bigger. (upr.org)
Just before birth, mice with the human DNA had brains that were noticeably larger - about 12 percent bigger than the brains of mice with the chimp DNA, according to a report in the journal Current Biology . (upr.org)
Mice were injected intravenously with human cfDNA and Cfs and analysis of heart, lung, liver, and brain of the mice sacrificed 7 days after injection revealed genomic localization of nucleic acids, with Cfs localizing more efficiently than cfDNA. (fightaging.org)
Now, in a study using mice and human tissue, scientists discovered that the medication locks some embryonic cells into a suspended state where they can't properly grow or divide. (livescience.com)
the same enzymes did not crop up in the cells of healthy, unexposed mice. (livescience.com)

They are still developing and they have the potential to become any of the major specialized cell types within that organ. (cbc.ca)
The scope of the systems covered ranges from cellular to tissue, organ, systems studies, and up to populational dynamics. (nih.gov)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)

Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
In this study we show that contactin coimmunoprecipitates with Na v 1.3 from postnatal day 0 rat brain where this channel is abundant, and from human embryonic kidney (HEK) 293 cells stably transfected with Na v 1.3 (HEK-Na v 1.3). (jneurosci.org)

Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
Variation in the policies across the globe is expected to impact the global human embryonic stem cell market development. (grandviewresearch.com)
They are in an early stage of development and have the ability to become any type of cell to form skin, bones, organs or other body parts. (cbc.ca)
Development of new or improved laboratory/experimental techniques, instruments, or supporting software that measure the location and dynamics of molecules in situ, and organelles, cells, or tissues on the nanometer and micrometer length scales. (nih.gov)
They play critical roles during embryonic development and in the maintenance and repair of adult tissues. (cshlpress.com)
We modeled the development of embryonic tissues from hPSCs and delineated the ontogeny of hematopoietic cells by mitochondrial variant lineage tracing. (biorxiv.org)
We further applied mitochondrial variant lineage tracing to spatial transcriptomics and identified the clonal development of both erythro-myeloid progenitors and their niche cells. (biorxiv.org)
Our study highlights the power of mitochondrial variants as an endogenous marker for clonal tracking in modeling human development from stem cells. (biorxiv.org)
In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
Furthermore, observations made in the retinal organoid model closely parallel those in human fetal retina further validating use of retinal organoid to model early retinal development. (caltech.edu)
Results of low-dose recombinant human insulin-like development factor-I on insulin sensitivity, development hormone and glucagon ranges in younger adults with insulin-dependent diabetes mellitus. (ncbcs.org)
Wnt proteins are key regulators of embryonic development. (medscape.com)

Ewen Callaway investigates a new field in cell biology. (nature.com)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
From 1999 onwards my research has been focused primarily on osteoblast biology and approaches to tissue engineer and model bone biology, pathology and repair. (nottingham.ac.uk)
My group works with translational stem cell biology. (lu.se)

Differential modulation of BMP Signaling by Activin/Nodal and FGF pathways in lineage specification of Human Embryonic Stem Cells. (mpg.de)
The ability to perform lineage tracing at the single-cell level is critical to reconstructing dynamic transitions during cell differentiation. (biorxiv.org)

In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
These circulating nucleic acids are thought to arise from the destruction of cells, though given that cells are capable of creating and releasing quite complex structures into the surrounding tissues - consider extracellular vesicles for example - it is perhaps plausible that dysfunctional cells could be exporting nucleic acids while still intact. (fightaging.org)

In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)

Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
Scientists have long suspected that even cells that are not traditionally involved in the immune response have the rudimentary ability to remember prior insults and learn from experience. (scitechdaily.com)
A number of scientists are trying to create life in the lab, specifically artificial cells. (reasons.org)
In Pollard's lab, scientists are exploring the differences between chimp and human DNA using dishes of cells. (upr.org)
This may allow scientists to culture healthy tissue cells in the lab to replace damaged ones in the human body. (latimes.com)
Scientists have found a way to possibly avoid using embryonic stem. (scitizen.com)
Scientists at Stanford University in California have been able to coax embryonic stem cells into becoming eggs and sperm, which could one day lead to the creation of children through entirely artificial means. (womenofgrace.com)
British scientists are hailing the recent creation of human sperm cells that they believe could revolutionize fertility treatment. (womenofgrace.com)

Tissues representing endoderm (alpha-fetoprotein(+)) and mesoderm (smooth muscle actin(+)) were not seen among GFP(+) tissues. (ca.gov)

Nowadays, bone marrow can be taken from people who are similar enough to the patient who needs the cells to prevent rejection. (wikipedia.org)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)

Stem cells are the progenitors of specialized cell types in the body and have the ability to self-renew. (cshlpress.com)
This population of 'border located undecided progenitors' (BLUPs) gradually diminishes as cell fate decisions take place. (bvsalud.org)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

We established the human embryonic organoid (HEMO) from expanded potential stem cells and achieved both embryonic and extraembryonic tissues in the same organoid. (newswise.com)

Ontario and California together account for about 70 per cent of the stem cell research currently conducted in North America. (cbc.ca)

The first clinical trials involving a patient receiving human embryonic stem cells began in October 2010 at the Shepard Center, a spinal cord injury hospital in Atlanta. (cbc.ca)
This patent approval is an important milestone as RepliCel's licensing partner, Shiseido Company, prepares to conduct human clinical trials using RCH-01. (baldingblog.com)
DBS is a widely used FDA-approved treatment and stem cell-derived dopamine neuron replacement has now evolved to the first in-human clinical trials. (lu.se)

One explanation, the Fuchs team proposed, could be epigenetic changes to the skin cell genome itself. (scitechdaily.com)
ERVs and related sequences are estimated at ~400,000 copies or 4.6% of the human genome, including genomic proviruses like 5' long terminal repeat (LTR)-gag-pol-env-3' LTR ( 1 ). (frontiersin.org)
And, using genome engineering techniques, we can start to study the effects of switching the human and the chimp sequences in these primate cell lines. (upr.org)

HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
or = 0.02 ng/ml of mGLP-1 facilitated cell proliferation and 0.1 ng/ml and 0.5 ng/ml of mGLP-1 rescued SH-SY5Y cells from Abeta(1-42)-induced apoptosis. (ncbcs.org)
On the other hand, for primary teeth, enzymatic digestion always promoted a higher cell proliferation. (bvsalud.org)

Regenerative medicine also includes the possibility of growing tissues and organs in the laboratory and implanting them when the body cannot heal itself. (wikipedia.org)
Some of the biomedical approaches within the field of regenerative medicine may involve the use of stem cells. (wikipedia.org)
These advances led to tissue engineering, and from this field, the study of regenerative medicine expanded and began to take hold. (wikipedia.org)
He recognized that these cells' unique ability to differentiate into all the cell types of the human body (pluripotency) had the potential to develop into a new kind of regenerative therapy. (wikipedia.org)
Applicability for inducing differentiation into a wide range of tissues is attributive to the largest share of regenerative medicine. (grandviewresearch.com)
He is also interim president of the state's new stem cell agency, the California Institute for Regenerative Research. (latimes.com)
Melton and the institute's other co-director, Professor of Medicine David Scadden, who is also director of Massachusetts General Hospital's Center for Regenerative Medicine and Technology, outlined the basics of stem cell research for the audience. (harvard.edu)
In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)

Hair growth in adults occurs naturally in a process known as hair neogenesis â€" where cells called dermal papilla cells that span the top two layers of skin coax surrounding cells to form hair follicles. (baldingblog.com)

In this study, we show that contactin associates with Na v 1.3 in P0 brain tissue and interacts with both the N and C termini of Na v 1.3. (jneurosci.org)
It's likely to be one of many DNA regions that's critical for controlling how the human brain develops," says Debra Silver , a neurobiologist at Duke University Medical School. (upr.org)
They're also looking for other bits of uniquely human DNA that affect the brain. (upr.org)
We can now actually generate the equivalent of embryonic brain cells and tissues that are human or chimpanzee. (upr.org)
These markers of senescence specifically appeared in exposed neuroepithelial cells, a type of stem cell that later produces brain cells. (livescience.com)
Among brain tumors , just 0.5 to 3 percent are germ cell tumors, and of these, about 20 percent are teratomas, Dehdashti said. (livescience.com)
The ultimate aim is to develop these cells and technologies for use in brain repair, with focus on Parkinson's disease. (lu.se)

David Scadden (below), co-director of the Stem Cell Institute, who also spoke at the symposium, chats with some conference attendees during a break. (harvard.edu)
Co-Director of the Stem Cell Institute Douglas Melton, who also spoke at the symposium, listens intently during a talk. (harvard.edu)
Correspondence to: Roger A. Barker, Department of Clini- jections are lost, it does not tell us that this leads to cal Neuroscience and WT-MRC Cambridge Stem Cell Institute, the expression of many of the features of PD. (lu.se)

Anatomy36

Organisms16

Diseases8

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes87

Information Science3

Health Care2

Differentiation of human4

Embryos21

Debate over embryonic stem2

Fetal tissue2

Pluripotent cells2

Organs and tissues4

Vitro13

Normal embryonic development3

Transplantation10

Vivo1

HESC6

Therapeutic12

Mesenchymal7

Abstract2

Progenitor4

Organoids7

Neuronal2

Neural9

Tumor tissues1

Molecular9

Injured or diseased1

Researchers11

Epithelial cell1

Embryo7

Germ layers2

Neurons8

Transplants2

Explants2

Characterization3

ESCs2

Therapies6

Physiology3

Regeneration1

Patient's2

Genetic9

Diseases12

Genes6

Self-renewal and differentiation1

Functional Genomics1

Developmental1

Mice7

Organ5

Kidney2

Development12

Biology4

Lineage2

Extracellular vesicles2

Adult human tissue1

Scientists10

Mesoderm1

Bone3

Gene expression1

Progenitors4

Organoid1

North America1

Clinical trials3

Genome3

Proliferation3

Regenerative9

Coax1

Brain7

Stem Cell Institute3