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  • connective tissue
  • Hearts were removed from the perfusion apparatus, ventricles were dissected free of aorta and connective tissue, and hearts were stored overnight at 4C in cryoprotectant solution (0.1 M phosphate buffer, 30% sucrose, 30% ethylene glycol). (histosearch.com)
  • Fine details of cytoplasmic filaments and organelles, membranes and membrane-associated structures, as well as connective-tissue elements are all visible. (biologists.org)
  • proteins
  • For over 20 years, BioChain has been a trusted source for tissues, as well as proteins, DNA, and RNA isolated from the materials in our tissue bank, to clinical and biopharmaceutical researchers. (biochain.com)
  • sperm
  • As such, the team thawed the testicular tissue after more than 4 months and applied the spermatogenesis technique, which successfully produced sperm. (medicalnewstoday.com)
  • samples
  • It's smaller than a child's lunchbox and could change the way tissue samples are cryogenically frozen in the lab. (hartfordbusiness.com)
  • Former Yale University researcher Dr. Dmitry Kravtsov, now vice president of research and development at Vanessa, developed the idea out of frustration with the amount of time his staff was spending freezing tissue samples individually. (hartfordbusiness.com)
  • Biochain has been in the business long enough to offer a thousands of different tissue samples, both healthy and diseased, from myriad organs and tumors from our existing bank of samples. (biochain.com)
  • When you and your team need a frozen tissue section for your research, BioChain is your best option for obtaining the highest quality samples. (biochain.com)
  • Total RNA was isolated from various frozen mouse tissue samples that were either processed directly from a frozen state or thawed in RNA later -ICE at -20ºC overnight. (thermofisher.com)
  • For each tissue the integrity of both rRNA and mRNAs for the RNAlater ICE treated samples is comparable to that of RNA prepared directly from frozen tissue samples. (thermofisher.com)
  • RNA Integrity from Frozen Samples Ground, Thawed or Treated with RNA later -ICE. (thermofisher.com)
  • Total RNA was isolated from mouse liver samples that were processed directly from a frozen state (ground), thawed on a benchtop for 5 min (thawed), or thawed overnight at -20ºC in RNA later -ICE and then stored at rt for 30 min prior to RNA isolation (treated). (thermofisher.com)
  • The goal of this study is to study NIS expression in benign and malignant breast and thyroid samples using archival formalin-fixed paraffin-embedded tissue sections. (clinicaltrials.gov)
  • Medium
  • He said standard procedures involve using forceps to hold the sample above a beaker of a freezing medium and repeating the process for each additional sample. (hartfordbusiness.com)
  • follicles
  • Investigate factors affecting successful maturation of immature follicles obtained from ovarian tissue including the use of 3-dimensional biogel scaffolds, growth factors, hormones and other culture conditions. (clinicaltrials.gov)
  • But the operation was a success - the grafted ovarian tissue responded to the woman's hormones, and began producing the little follicles needed to release maturing eggs. (upriser.com)
  • puberty
  • A 24-year old has become the first woman to give birth after a transplant of ovarian tissue that was taken before puberty. (bionews.org.uk)
  • Mrs Moaza Al Matrooshi, from Dubai, gave birth in London on 13 December, after surgeons implanted ovarian tissue taken when she was nine years old - before puberty - and this appears to be a world first. (planer.com)
  • isolation
  • Once tissues are thawed they can be easily processed using standard RNA isolation procedures. (thermofisher.com)
  • No more laborious grinding of frozen tissue to preserve RNA in difficult tissues or tissues that need to be stored prior to isolation. (thermofisher.com)
  • Once treated, tissue can be safely stored at 4ºC or even at room temperature (for a limited period of time) and can be further dissected or processed prior to homogenization in a standard RNA isolation lysis buffer. (thermofisher.com)
  • Here we compare RNA that was prepared directly by homogenization of frozen tissue, with a sample that was allowed to thaw at room temperature for 5 minutes, and a sample that was frozen, soaked overnight at -20ºC in RNA later -ICE, and kept at room temperature for 30 minutes before being processed for RNA isolation. (thermofisher.com)
  • option
  • So if you want to have a child in the future, but have not had the opportunity during your most fertile years, then freezing your eggs for use in the future - when you have met the right partner, or are ready to start your family - may be an option for you. (internationalfertilitycentre.com)
  • fresh
  • i never had this "bubble" problem in the past and i had no problem to isolate RNA from fresh unfixed tissue or from cellculture. (protocol-online.org)
  • years
  • The tissue induced a hormonal response, causing her to begin menstruation after five months, and she conceived naturally two years later at the age of 27. (bionews.org.uk)
  • healthy baby
  • Through cryopreserving testicular tissue and using an organ culture system that triggered spermatogenesis, the team was able to produce eight healthy baby mice. (medicalnewstoday.com)