Tissue processing tissue fixation. Medical search. Frequent questions

The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. (qiagen.com)
This online seminar focused on recent advances in gene expression profiling of challenging formalin-fixed, paraffin-embedded tissue samples for cancer research and clinical practice. (genomeweb.com)
Ipswich, MA (January 23, 2014) - New England Biolabs (NEB®) announces a novel DNA repair reagent aimed at helping to address the historically intractable challenge of sequencing DNA from archival, formalin-fixed, paraffin-embedded (FFPE) tissue samples. (neb.com)
Advances in the field of molecular biotechnology have made it possible to extract proteins from formalin fixed and paraffin embedded (FFPE) tissue blocks. (cytojournal.com)
We run FISH her 2 on formalin fixed paraffin embedded tissue as per FDA protocol. (utsouthwestern.edu)
Also, we are a regional reference lab for IHC and have a panel of 115 antibodies, all optimized for formalin fixed paraffin embedded tissue. (utsouthwestern.edu)
Background: Formalin-fixed paraffin-embedded (FFPE) tissue archives in hospitals, biobanks, and others offer a vast collection of extensive, readily available specimens for molecular testing. (bvsalud.org)
Formalin-fixed paraffin-embedded tissue samples were collected and archived from autopsies conducted over 13 years in the forensic medicine department of the University of Pretoria (Pretoria, South Africa). (bvsalud.org)

Certain specimens (especially biopsies) can undergo agar pre-embedding to assure correct tissue orientation in cassette & then in the block & then on the diagnostic microscopy slide. (wikipedia.org)
Fixation times for very small specimens are shorter, and standards exist in human diagnostic histopathology. (wikipedia.org)
The PAXgene Tissue FIX Container (50 ml) enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). (qiagen.com)
PAXgene Tissue STABILIZER is used for transport or storage of tissue specimens fixed with the PAXgene Tissue FIX Container (50 ml). (qiagen.com)
PAXgene Tissue STABILIZER Concentrate is only to be used in conjunction with tissue specimens previously fixed in PAXgene Tissue FIX. (qiagen.com)
The tissue specimens included lon campechianum [ 1 ]. (who.int)
Diagnostic neuropathology is based on the same principles used for the preparation and evaluation of tissue specimens used in Anatomic Pathology. (chop.edu)
The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of miRNA, total RNA, or DNA. (qiagen.com)
But Steven Gonias, chairman of the department of pathology at the University of California San Diego, takes issue with Topol's characterization of the pathology field, noting that his department has "invested in new biorepository facilities so that all of our diagnostic specimens can be stored and processed by conventional methods and by multiple state-of-the-art tissue-freezing methods, facilitating techniques like genome sequencing. (genomeweb.com)
Objective -To determine whether metal concentrations in canine liver specimens were influenced by specimen size, assay variability, tissue processing (formalin fixation and deparaffinization), or storage in paraffin blocks. (avma.org)
Procedures -Fresh frozen liver specimens (obtained via simulated needle-core and wedge biopsy), fresh hepatic tissue, and deparaffinized-archived specimens (0.5 to 14 years old) were analyzed for concentrations of copper, iron, and zinc by atomic absorption flame spectrometry. (avma.org)
In tissues with high metal concentrations, copper and iron concentrations were significantly lower in needle-core versus wedge biopsy specimens. (avma.org)
A higher zinc concentration in deparaffinized-archived specimens masked a low zinc concentration in fresh liver tissue of 10 of 20 (50%) dogs. (avma.org)
MFPE blocks were made by Cellient™ automated system using human tissue specimens from normal and malignant specimens collected in ThinPrep™ Vials. (cytojournal.com)
In the current era of minimally invasive techniques for obtaining tissue samples for diagnostic and prognostic markers, cellblocks containing "limited material" specimens are routinely used to provide the valuable information about pre-disease and disease processes. (cytojournal.com)
4. All requests for specimens from cases with the "Limited" status will require further justification and nPOD Tissue Prioritization Committee approval prior to distribution. (npod.org)

Our third generation of tissue valve technology brings you AOA ® tissue treatment, the Physiologic Fixation™ process, and now, the Cinch ® Implant System - demonstrating our commitment to innovation. (medtronic.com)
1,2,3 The Physiologic Fixation process, used in Medtronic's third generation tissue valves, was developed to address the problem of structural valve deterioration caused by mechanical stress and is designed to maintain native collagen structure and porcine aortic root and leaflet geometry. (medtronic.com)
No clinical data is available which evaluates the long-term impact of the Physiologic Fixation process or the impact of AOA treatment in patients. (medtronic.com)

PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
PAXgene Tissue Containers provide tissue fixation for histopathology studies and enable purification of high-quality nucleic acids from the same sample for molecular analysis. (qiagen.com)

Most embedding waxes are fairly quick infiltrates that can accommodate various sizes and density in tissue specimen (breast, bone, etc. (labstore.com)
Specifically, in clinical medicine, histopathology refers to the examination of a biopsy or surgical specimen by a pathologist, after the specimen has been processed and histological sections have been placed onto glass slides. (wikipedia.org)
however, longer fixation does not change the dimensions of the specimen. (uni-koeln.de)

The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. (genetex.com)
There were four time points investigated: directly after excision in the operating room and after four, 24 and 72 h of fixation in formaldehyde (4% Formaldehyde in phosphate buffer pH 7.4). (uni-koeln.de)

The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers. (qiagen.com)

The most commonly used stain in histology is a combination of hematoxylin and eosin (often abbreviated H&E). Hematoxylin is used to stain nuclei blue, while eosin stains the cytoplasm and the extracellular connective tissue matrix of most cells pink. (wikipedia.org)
One of the most used staining methods for tissue slides is hematoxylin and eosin (H&E) staining. (thebiogirls.com)

The organotypic culture procedure, which preserves testicular tissue architecture, microenvironment and cell interactions, has been used successfully to obtain spermatozoa from fresh or frozen/thawed mouse prepubertal testicular tissues ( 3 - 6 ). (elifesciences.org)
The tissue is then prepared for viewing under a microscope using either chemical fixation or frozen section. (wikipedia.org)
The second method of histology processing is called frozen section processing. (wikipedia.org)
In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. (wikipedia.org)
This can be done to slides processed by the chemical fixation or frozen section slides. (wikipedia.org)
The Scripps physicians recommend that cancer tissue samples be frozen, rather than fixed in formalin, to improve tissue processing for whole-genome sequencing. (genomeweb.com)
However, "discovering this level of crucial detail is possible only with whole genome and exome sequencing of frozen tissue. (genomeweb.com)
The authors acknowledge that procuring frozen samples will add an extra step to the current process of formalin fixation and also may require obtaining additional tissue. (genomeweb.com)
Topol tells the Union-Tribune that most pathologists "are very much against" procuring frozen tissue due to the additional expense of storing the samples and the lack of reimbursement to do so. (genomeweb.com)
Recommend the use of acetone fixation for frozen sections. (genetex.com)

Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. (qiagen.com)
Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. (qiagen.com)
During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. (qiagen.com)
This is especially true if the starting material is FFPE tissue, where the fixation process introduces further degradation of the RNA. (genomeweb.com)

Histopathological examination of tissues starts with surgery, biopsy, or autopsy. (wikipedia.org)
At the Histology & Research Services (HRS) Laboratory, we offer a full range of custom and routine histological solutions to assist in your resarch, from animal autopsy, tissue dissection and fixation, to accurate processing, sectioning, and staining. (dal.ca)

After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. (qiagen.com)
PAXgene Tissue FIX Container (50 ml) is only to be used in conjunction with PAXgene Tissue STABILIZER. (qiagen.com)
Specifications for fixation and storage conditions in PAXgene Tissue FIX and PAXgene Tissue STABILIZER were determined using animal tissues. (qiagen.com)
The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. (qiagen.com)
After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. (qiagen.com)

Next, Bryan Lo of the Ottawa Hospital discussed a method for gene expression profiling of pancreatic cancer precursors directly from FFPE tissues without nucleic acid extraction. (genomeweb.com)
Dr. Lo and colleagues have shown in a pilot study that they can use the HTG EdgeSeq Oncology Biomarker Panel to profile approximately 2,500 genes from a cohort of microdissected low- and high-grade PanIN lesions from human pancreatic cancer resections that have been archived as FFPE tissue blocks. (genomeweb.com)
The NEBNext FFPE DNA Repair Mix makes it possible to apply today's and tomorrow's next-generation sequencing (NGS) technologies even to compromised FFPE tissue samples, which we hope will open up new avenues in the analysis of these valuable samples. (neb.com)
thus, the forensic pathology FFPE tissue archives in Africa have been a largely unexploited genetic resource, with the usability of DNA obtainable from these samples being unknown.Intervention: The study, conducted from January 2015 to August 2016, determined the usefulness of FFPE tissue as a reliable source of genetic material for successful post-mortem molecular applications and diagnostics. (bvsalud.org)
Deoxyribonucleic acid from FFPE tissue samples and control blood samples was amplified by high-resolution melt real-time polymerase chain reaction before sequencing. (bvsalud.org)

Freezing of prepubertal testicular tissue containing spermatogonia is a fertility preservation option proposed to prepubertal boys with cancer prior to highly gonadotoxic treatments. (elifesciences.org)
Preservation of tissue morphology. (qiagen.com)
the paraffin both promotes tissue preservation and enables tissue sectioning. (neb.com)

While the fixation process adequately preserves the ultrastructure of the tissues, it often results in various types of damage to the DNA within the tissues, including deamination of cytosine to uracil, generation of nicks and gaps, oxidation of bases and blocking of 3´ ends, as well as fragmentation and crosslinking of the DNA. (neb.com)

Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality miRNA and total RNA for molecular analysis. (qiagen.com)
For purification of total RNA including miRNA, the system requires the use of PAXgene Tissue Containers for tissue collection and stabilization, followed by RNA isolation and purification using the PAXgene Tissue miRNA Kit. (qiagen.com)

In the first step, dehydration, placing tissue directly into pure dehydrant can and mostly likely will result in cellular disruption where the cell membrane rapidly shrinks forming folds, wrinkles and distorting the natural morphology. (labstore.com)
PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. (qiagen.com)

Under-dehydration can result in residual bound water in tissue blocking subsequent reagents, inconsistency in tissue profile, and soft and mushy center. (labstore.com)
PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. (qiagen.com)
I know that at least some of the microwave processing done out there is being done with 'proprietary' reagents and not with formalin. (utsouthwestern.edu)

Histopathology (compound of three Greek words: ἱστός histos 'tissue', πάθος pathos 'suffering', and -λογία -logia 'study of') refers to the microscopic examination of tissue in order to study the manifestations of disease. (wikipedia.org)

In addition, archived tissue samples can be very small, and may only contain trace amounts of the cells of interest, and therefore the nucleic acid of interest. (neb.com)

The tissue is removed from the body or plant, and then, often following expert dissection in the fresh state, placed in a fixative which stabilizes the tissues to prevent decay. (wikipedia.org)
Formalin is commonly used as a fixative for the long-term storage of clinically-important tissue samples. (neb.com)

We investigated commonly available CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate) buffer, as well as two commercially available Qiagen ® kits and compared their effectiveness on MFPE tissue for protein yields. (cytojournal.com)

A rare cause of this situation is the intestinal band caused by remaining fibrous connective tissue leading to entrapment and consequent intestinal obstruction. (vin.com)
The parameters evaluated were: intensity and kind of inflammatory infiltrate, presence of connective tissue, foreign body reaction, vascularization and biodegradation. (bvsalud.org)
and after 9 weeks there was minor biodegradation associated with the presence of new connective tissue, and persistence of moderate inflammatory reaction observed from beginning to end of the experiment. (bvsalud.org)

In this article we will discuss the post fixation steps in routine histological tissue processing (dehydration, clearing, infiltration), and how they must be administered and maintained to achieve complete and optimal processing. (labstore.com)
Over-dehydration can result in fragmentation of the molecular bond in the tissue, disintegration of the protein molecule, and brittle fragmentation (flakes) during microtomy. (labstore.com)
Other techniques involve dehydration in alcohols and infiltration with paraffin, or some similar agent - a process called embedding. (columbia.edu)

The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. (qiagen.com)
process, tissue fixation methods, pH and 1. (who.int)
Current tissue fixation methods used in traditional histology are of limited use for molecular analysis. (qiagen.com)
Material and methods: In order to obtain comparable tissues, we investigated 100 palatal tonsils after cold steel dissection tonsillectomy for chronic tonsillitis. (uni-koeln.de)

Yet the potential for discovering clinically actionable driver and passenger mutations and also for analyzing tumor heterogeneity suggest consideration for more rather than less tissue procurement. (genomeweb.com)
Samples are recovered into UW solution and transported to the organ procurement and pathology core via tissue donor courier services. (npod.org)

Hypothesis: Formalin fixation may lead to shrinkage in tissue dimensions and may thus alter tumor stages. (uni-koeln.de)
Conclusion: The null hypothesis has to be withdrawn that tissue dimensions are altered by formalin fixation. (uni-koeln.de)
A fail in the process of intestinal rotation and fixation the happen in early stage of embryogenesis, together with cartilaginous tissue organization, is a possible hypothesis. (vin.com)

and third, to infiltrate the tissue with an embedding medium (infiltration wax) that will give the tissue/cells the structural support needed to withstand the thin sectioning of microtomy. (labstore.com)
The final step, infiltration, serves to give structure and support to cells and tissue stroma to keep the tissue in proper relationship during microtomy. (labstore.com)
In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
In work with the light microscope, it is difficult to recognize the various components of cells and tissues without differential staining. (columbia.edu)
Differentiation of cells present in hematopoietic tissue. (studystack.com)
The nuclei of cells samples from the endometrium, appendix, are stained by its active product, haematin, gall bladder, spleen and other gastric and which is produced when an oxidizing agent pleural tissues obtained by surgery, curet- is added. (who.int)
Thus, GPI-anchored FGF organizes both source and recipient cells and self-regulates its cytoneme-mediated tissue-specific dispersion and signaling. (biorxiv.org)
During development, intercellular communication of morphogens is critical for embryonic cells to determine their positional identity, directionality, and interactions in an organized pattern to sculpt tissue. (biorxiv.org)
Understanding how signals might inform cells of their positional identity, directionality, and interactions and organize these functions in diverse tissue-specific patterns is critical to understanding morphogenesis. (biorxiv.org)
The discrete tissue-specific organization of morphogen signaling is known to be dependent on the ability of signal-receiving cells to selectively sense and respond to a specific signal 3 . (biorxiv.org)
Fixation is considered as physiochemical process where cells or tissues are fixed chemically. (ls-lev.de)
Tissues are groups of cells that work together to perform a specific function. (alloptica.com)
These microscopes can observe a variety of materials including cells, tissues, and bacteria. (alloptica.com)
Both are based on four basic principles: biocompatibility, maintenance of adequate space to support the newly formed tissue, the promotion of growth of osteogenic cells and peripheral sealing to prevent growth of unwanted cellular tissue inside the defective area and the displacement of the membrane 3-5 . (bvsalud.org)

MCPyV), has been recently identi- real-time RT-PCR assay for detection of fi ed in tumor tissue from patients with 308, and 351 bp, respectively. (cdc.gov)
The Children's Hospital of Philadelphia Pediatric Neuropathologists also make significant contributions to translational and basic research at Children's Hospital of Philadelphia Research Institute by contributing to the Pathology Core Laboratory and supporting an extensive multi-institution pediatric brain tumor tissue repository. (chop.edu)
An IHC can identify characteristics of tumor tissue that provide clues about how cancer may respond to treatment. (labtestzote.com)

The full root, stentless design of the Freestyle™ porcine bioprosthesis - based on more than 30 years of Medtronic tissue valve design improvements - can be used for full root, modified subcoronary, and complete subcoronary configurations. (medtronic.com)

To see the tissue under a microscope, the sections are stained with one or more pigments. (wikipedia.org)
In order to study tissues with a microscope they must be preserved (fixed) and cut into sections thin enough to be translucent. (columbia.edu)
With a light microscope, you can observe the different types of tissues in plants and animals: epithelial, connective, muscular, and nervous tissues. (alloptica.com)

Clusterin is a highly conserved glycoprotein ubiquitously expressed in the brain as well as in peripheral tissues in different species, including rodents and humans 15 . (nature.com)

Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
Discussion: Formalin fixation does not significantly influence the tissue dimensions of palatal tonsils in comparison to direct ex vivo measurements. (uni-koeln.de)

Several fertility restoration approaches, whose aim is to mature cryopreserved tissues in vivo or in vitro in order to produce spermatozoa, are being developed. (elifesciences.org)
Background: It is often observed in tumor surgery that the dimensions in vivo seem larger than after resection, and tissue appears to shrink further after formalin fixation. (uni-koeln.de)

To study the effect of tap water `softening' (i.e. calcium extraction) on H&E stains, 5 sets of slides from 30 different paraffin-embedded human pathologic tissue blocks were prepared in the same way except for washing with 5 different types of water. (who.int)
Subject: RE: [Histonet] Microwave processing We have the Sakura Xpress and we are still doing side-by-side comparisons on a variey of tissue types, IHC testing and special stains. (utsouthwestern.edu)
The material was implanted into subcutaneous tissue of mice (n=15) and after 1, 3 and 9 weeks, the animals were sacrificed and biopsies analyzed with light microscopy, using the stains hematoxylin-eosin, picrosirius and alcian blue pH 2.5. (bvsalud.org)

This study reports useful information on the limits of the organotypic culture of neonatal mouse testes, which has been regarded as an experimental strategy that can be extended to humans in the clinical setting for the conservation and subsequent re-use of testicular tissue. (elifesciences.org)
No abnormalities in clinical signs, terminal body and organ weights, or hematologic and serum chemistry were noted, and no gross or histopathologic lesions of systemic tissue toxicity were found in any treatment group at any time point studied. (hindawi.com)
Clinical severity scores were assigned on the basis of tissue metal concentrations. (avma.org)
Cytology is a useful clinical tool for investigation of disease processes, and the techniques and their interpretation have developed into an entire discipline. (merckvetmanual.com)

These services are provided by the Organ Processing and Pathology Core (OPPC). (npod.org)

La teneur faible en ions calcium et magnésium et élevée en ions sodium de l'eau douce affecte négativement les résultats de la coloration de routine à l'hématoxyline-éosine. (who.int)
As I tech, I am not in favor of tossing routine processing wholesale in favor of a completely new technology without thorough testing and parallel processing. (utsouthwestern.edu)

Optimal tissue embedding is essential for smooth sectioning and ribboning. (leicabiosystems.com)
The preceding article (Optimal Fixation), discussed fixation as the foundation of tissue processing, upon which the success of all other steps is dependent. (labstore.com)
Aim: To investigate the change of tissue dimensions after formalin fixation, and to determine the optimal time of fixation. (uni-koeln.de)

Mammary tissues from C57BL6/J strain mice (8 wk of age) were prepared by perfusion fixation, processed as described previously (Nakatani et al. (ucsd.edu)

Although advances in tissue typing and cross-match testing have helped to deter severe transplant rejection, rejection may result from direct cellular attack (cellular rejection) or be mediated by antibodies (humoral rejection). (medscape.com)
After that, you can remove the primary and secondary antibodies, for instance by microwaving the tissue, followed by a restain with the next antibody and fluorochrome combination. (thebiogirls.com)
Immunohistochemistry (IHC) is a valuable laboratory diagnostic technique that employs antibodies to detect antigens in a tissue sample. (labtestzote.com)
Immunostaining involves using antibodies and special markers to "label" parts of a tissue sample so they're easier for pathologists to identify. (labtestzote.com)
An IHC uses the antigen-finding properties of antibodies to detect and grab onto antigens in a tissue sample. (labtestzote.com)

1% sodium bicarbonate (NaHCO ) dis- ions in tissues such as collagen or muscle. (who.int)
Y-27632 eye drops (or vehicle only) were administered to eyes after a superficial keratectomy, and the tissue was examined by immunohistochemistry for α-SMA, collagen types I, II, and III, and keratan sulfate. (molvis.org)
Interestingly, bundles of aligned and uniformly spaced collagen fibrils were more prevalent in keratocytes in Y-27632-treated corneas, which is reminiscent of fibripositor-like structures that have been proposed as a mechanism of matrix deposition in embryonic connective tissues. (molvis.org)

An initial evaluation of a suspected lymphoma is to make a "touch prep" wherein a glass slide is lightly pressed against excised lymphoid tissue, and subsequently stained (usually H&E stain) for evaluation under light microscopy. (wikipedia.org)
Haematoxylin stain is extracted from formalin, were processed routinely for his- the logwood of a tropical tree, Haematoxy- topathology. (who.int)
I have been pro microwave processing since they hit the marketplace, and am anxious to purchase one, but the info not to stain IHC from one has me worried. (utsouthwestern.edu)

The staining method can be altered to suit the needs of the examiner in order to accentuate certain tissues or organelles. (columbia.edu)

In contrast, traditional models predict that the signal presentation from the source via free secretion and extracellular diffusion is a non-selective process. (biorxiv.org)

You can also analyze the structure and organization of tissues. (alloptica.com)

tissue samples by using the QIAamp goza J, de Ory F, Minguell S, et al. (cdc.gov)
Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA. (qiagen.com)
In a Viewpoint column in this week's Journal of the American Medical Association , Eric Topol, Kelly Bethel, and Laura Goetz of the Scripps Translational Science Institute and the Scripps Clinic say that pathologists must change the way they handle tissue samples in order to take full advantage of genomic sequencing in cancer care. (genomeweb.com)
Tissue from donors who do not have type 1 diabetes is also recovered to provide critical controls for the autoantibody positive and type 1 diabetes samples. (npod.org)

PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. (qiagen.com)
Total RNA purified using the PAXgene Tissue miRNA Kit is highly pure. (qiagen.com)

PAXgene Tissue FIX Containers (50 ml) are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. (qiagen.com)

Following fixation, blocks of tissue must be cut into thin sections. (columbia.edu)
Chemistry studies the tiny building blocks that make up all matter, including human tissue. (labtestzote.com)

Finally, in order to preserve the section which has been made from a block of fixed tissues and stained, it is mounted on a glass slide and covered with a thin cover glass by means of a transparent substance which hardens and seals the preparation to make it permanent. (columbia.edu)

nPOD provides tissue recovery services for pancreata and related tissues from autoantibody positive donors and donors with type 1 diabetes. (npod.org)

Xylene is the best solution for basic tissue processing, and since we are discussing this interstitially at the cellular level, time and multiple solution changes are again critical factors for complete removal of dehydrant. (labstore.com)

The latest addition to Medtronic's tissue valve portfolio, 3f ® Aortic Bioprosthesis, is the only pericardial tissue valve designed to function like a native human aortic valve. (medtronic.com)
Pressure fixation of valves can compromise the function and durability of the bioprosthesis. (medtronic.com)

In the current era of minimally invasive techniques to obtain minimal amount of tissue for diagnostic and prognostic purposes, the use of commercial and lab made buffer on low weight MFPE scrapings obtained by Cellient ® processor opens new possibilities for protein biomarker research. (cytojournal.com)

The subsequent burial of the wood in silty sediment led to a silicification of the cellular tissue. (researchgate.net)
silicification of the cellular tissue. (researchgate.net)

Fundamentally it consists of a chemical or physical method of killing the tissue and yet retaining characteristic peculiarities of shape and structure. (columbia.edu)
The framework is made up of a number of modules (or submodels), each containing formulations of a relatively well-defined, related subset of ecosystem processes with a distinct spatial and/or temporal signature (characteristic scale). (lu.se)

All Institutional and Federal requirements for human tissue banking are current. (npod.org)

In conclusion, this study reports the failure of adult Leydig cell development and altered steroid production and signaling in tissue cultures. (elifesciences.org)
Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
Increased levels of POGLUT2 were found in multiple types of cancer tissues and cell lines. (frontiersin.org)

How signaling proteins generate a multitude of information to organize tissue patterns is critical to understanding morphogenesis. (biorxiv.org)
At the ribosome, the processed mRNA is translated to produce proteins from amino acid units. (cdc.gov)

Gene expression analysis of pancreatic intraepithelial neoplasia's (PanINs), the classical pancreatic cancer precursor, has been challenging because extracting and purifying RNA from pancreatic tissue is difficult. (genomeweb.com)

You use a secondary antibody conjugated with an enzyme, and add tyramide-conjugated fluorochromes that deposit on the tissue. (thebiogirls.com)

AOA treatment is Medtronic's biochemical approach to mitigating calcification in the wall and leaflets of tissue valves. (medtronic.com)

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes2

Formalin-fixed8

Specimens16

Physiologic Fixation3

Purification of high-quality nucleic acids2

Specimen3

Formaldehyde2

Fixed and stabilized1

Hematoxylin and eosin2

Frozen10

Degradation4

Autopsy2

PAXgene Tissue STABILIZER5

FFPE5

Preservation3

Preserves1

Stabilization2

Morphology2

Reagents3

Histopathology1

Nucleic1

Fixative2

QIAGEN1

Connective tissue3

Dehydration3

Methods4

Procurement2

Hypothesis3

Cells14

Tumor tissue3

Stentless1

Microscope3

Peripheral1

Measurements2

Vivo2

Stains3

Clinical4

Pathology1

Routine2

Optimal3

Mice1

Antibodies5

Collagen3

Stain3

Organelles1

Extracellular1

Organization1

Samples4

MiRNA2

Containers prefilled1

Blocks2

Preparation1

Donors1

Xylene1

Bioprosthesis2

Minimal1

Cellular tissue2

Characteristic2

Human1

Cell3

Proteins2

Pancreatic1

Antibody1

Leaflets1