Loading...
  • dilution
  • Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling HuC + HuD with ab184267 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ( ab97051 ) at 1/500 dilution. (abcam.com)
  • SwissProt
  • This band was subjected to digestion, peptide extraction, and sequence analysis using MALDI-MSMS, and the protein was identified as cytoplasmic actin with a decisive score (human SwissProt database, 60% protein coverage using the 15 highest scoring peptide groups and two lower scoring but acceptable peptides). (hindawi.com)
  • pHPL
  • 15,16 This protocol presents a practicable procedure to generate a pool of human platelet lysate (pHPL) derived from routinely produced platelet rich plasma (PRP) of forty to fifty single blood donations. (jove.com)
  • The implementation of pHPL into standard culture protocols represents a promising tool for further development of cell therapeutics propagated in an animal protein-free system. (jove.com)
  • Umbilical Cord
  • Comparative Characterization of Cells from the Various Compartments of the Human Umbilical Cord Shows that the Wharton's Jelly Compartment Provides the Best Source of Clinically Utilizable Mesenchymal Stem Cells. (jove.com)
  • The human umbilical cord (UC) is an attractive source of mesenchymal stem cells (MSCs) with unique advantages over other MSC sources. (jove.com)
  • autologous
  • Considering autologous MSC therapy in human renal disease, studies identifying uremia-associated mechanisms that account for altered MSC function are urgently needed. (jove.com)
  • platelet
  • The trophic effect of platelet derived growth factors has already been tested in several trials for tissue engineering and regenerative therapy. (jove.com)
  • testis
  • Furthermore, using western blotting in combination with sensitive detection methods, we were also unable to detect G72 protein in a number of human brain regions (including cerebellum and amygdala), spinal cord or testis. (biomedcentral.com)
  • Multiple transcripts for each gene have been cloned by reverse transcription-PCR from human brain, spinal cord and testis cDNA libraries. (biomedcentral.com)
  • Heart
  • We measured CA expression in hypertrophied human hearts to begin to examine the role of carbonic anhydrase in progression of human heart failure. (biomedcentral.com)
  • This could lead to production of strong tissue with properly organized collagen fibers, which is important for heart valve tissue engineering. (sigmaaldrich.com)
  • UniProt
  • Synthetic peptide, corresponding to residues within Human Pyruvate Dehydrogenase E1 beta subunit (UniProt: P11177). (abcam.com)
  • Sequence
  • Recombinant fragment corresponding to Human Fibulin 5 aa 250 to the C-terminus (internal sequence). (abcam.com)
  • Synthetic peptide corresponding to Human TGIF2 (internal sequence). (abcam.com)
  • Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. (abcam.com)
  • Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cytochrome P450 3A5 aa 250-350. (abcam.cn)
  • Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Amyloid Precursor Protein aa 750 to the C-terminus. (abcam.co.jp)
  • Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human G protein alpha Inhibitor 2. (abcam.com)
  • Cellular
  • Because cellular therapeutics should eventually find their way from bench to bedside we established a new method to isolate and further expand progenitor cells without the use of animal protein. (jove.com)
  • actin
  • A threefold increase in duplication rate was found when human, saphenous vein-derived myofibroblasts were cultured in PL, whereas expression of marker proteins (alpha-smooth muscle actin, vimentin, desmin, and nonmuscle myosin heavy chain) was similar. (sigmaaldrich.com)
  • Gene
  • Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses. (abcam.com)
  • No homology was found between any of the putative protein products from the transcripts of either gene and any known protein. (biomedcentral.com)
  • Species
  • Since these methods use only 8-20 ml human blood they have potential for monitoring reactive oxygen species generation and bioenergetics in a clinical setting. (jove.com)
  • vitro
  • A specific band for PPAR γ , (calculated molecular weight for human PPAR γ 1 = 54.55 kD) was identified on our Western blots by performing new experiments in which in vitro translated human PPAR γ 1 (provided by J. Peters, Pennsylvania State University) was compared with human fetal tissue lysates (Figure 1 ). (hindawi.com)
  • The Western blots of the fetal tissues were all reanalyzed using the ~55 kD band that aligned with the in vitro translated human PPAR γ 1. (hindawi.com)
  • normal
  • IHC-P: Human normal liver tissue. (abcam.cn)
  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. (abcam.cn)
  • IHC image of Sprouty 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. (abcam.cn)
  • The objective of this study was to carry out the first comprehensive analysis of adrenal steroid levels and steroidogenic enzyme expression in normal second trimester human fetuses. (springer.com)
  • Normal development and function of the fetal adrenals is also essential for several processes that can affect the fetus itself or the health of the neonate. (springer.com)
  • muscle biopsy
  • These can be isolated from human muscle biopsy samples using enzymatic digestion and their myogenic properties studied in culture. (jove.com)
  • Isolation
  • Here we detail the methods for the isolation and plating of monocytes, lymphocytes, neutrophils and platelets, without activation, from human blood and the analysis of mitochondrial bioenergetic function in these cells. (jove.com)