• While still found in brain tissue at higher concentrations than the majority of other brain proteins, LHFPL2 displays an abundance in platelets that is 60 times greater, in the top 10% of platelet proteins. (wikipedia.org)
  • Here, we discovered that ribotoxic stress induces a profound reorganization of NSs with enhanced recruitment of factors required for splice-site recognition, including the RNA-binding protein TIAR, U1 snRNP proteins and U2-associated factor 65, as well as serine 2 phosphorylated RNA polymerase II. (bvsalud.org)
  • Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
  • The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • Polycomb Group (PcG) proteins are a family of protein complex that regulate gene expression, especially repressing gene transcription [ 1 ]. (hindawi.com)
  • Alternative splicing of gene can generate multiple transcripts and proteins to regulate tissue and organ development [ 17 ]. (hindawi.com)
  • The cartilage-specific localization pattern of these novel TGF-β superfamily members, which contrasts with the more ubiquitous presence of other BMP family members, suggests a potential role for these proteins in chondrocyte differentiation and growth of long bones. (elsevierpure.com)
  • Shi D, Cheng X, Saquet A, Grifone R. RNA-Binding Proteins and Inner Ear Hair Cell. (encyclopedia.pub)
  • RNA-Binding Proteins and Inner Ear Hair Cell" Encyclopedia , https://encyclopedia.pub/entry/30958 (accessed December 05, 2023). (encyclopedia.pub)
  • RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)
  • LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
  • This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. (cancerindex.org)
  • We evaluated a next generation therapeutic peptide, HTL-001, capable of targeting HOX gene over-expression in GBM by disrupting the interaction between HOX proteins and their co-factor, PBX. (biomedcentral.com)
  • These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
  • Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
  • Once target genes were identified, IHC, qPCR and DSP were performed to explore differential expression in BG vs. DGAST tumors. (biochain.com)
  • These findings indicate miR-34a along with its putative target genes could play a role in RCC tumorigenesis and progression. (hindawi.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Fluorescence in situ hybridization fish is a cytogenic technique used for the detection and localization of rna sequences within tissues or cells 116. (web.app)
  • Fluorescence in situ hybridization fish preparation of fish probe recommended filter set fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
  • It has emerged as a powerful cytogenetic method for the analysis of cells and tissues on the transcriptome and genome level with more than 6 publications in the literature per day since the late 1990s fig. Fluorescence in situ hybridization fact sheet itrc. (web.app)
  • Fluorescence in situ hybridization fish is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. (web.app)
  • We studied by in situ hybridization and RNA blots the pattern of 9E3 mRNA distribution in the wings of normal, wounded, and RSV-infected newly hatched chicks. (silverchair.com)
  • In situ hybridization and immunostaining of sections from human embryos showed that CDMP-1 was predominantly found at the stage of precartilaginous mesenchymal condensation and throughout the cartilaginous cores of the developing long bones, whereas CDMP-2 expression was restricted to the hypertrophic chondrocytes of ossifying long bone centers. (elsevierpure.com)
  • Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections. (molvis.org)
  • RNA pull down assay, luciferase reporter assay and fluorescence in situ hybridization were applied to verify the interaction between BCRC-3 and microRNAs. (biomedcentral.com)
  • See the in situ hybridization pattern in Kohara NextDB . (nih.gov)
  • In particular, ribotoxic stress causes targeted excision of retained introns from pre-mRNAs of immediate early genes (IEGs), whose transcription is induced during the stress response. (bvsalud.org)
  • In the latest genome annotation, lncRNAs, which are arbitrarily defined as noncoding RNAs longer than 200 nucleotides, constitute ~72% of the transcribed genome 13 , whereas mRNAs comprise only 19%, indicating the need for functional annotation of lncRNAs. (nature.com)
  • They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
  • Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
  • Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)
  • Alternative processing of nascent mRNAs is widespread in eukaryotic organisms and greatly impacts the output of gene expression. (stanford.edu)
  • In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
  • The expression levels of circRNAs, mRNAs and miRNAs in human tissues and cells were detected by quantitative real-time PCR (qRT-PCR). (biomedcentral.com)
  • Some circRNAs contain miRNA response elements (MREs) and function as competing endogenous RNAs (ceRNAs) to interact with miRNAs and regulate the expression of target mRNAs. (biomedcentral.com)
  • CiRS-7 has more than 70 miR-7 binding sites and thus acts as effective miR-7 suppressor to regulate the expression of miR-7 target mRNAs. (biomedcentral.com)
  • AceView: gene:dpy-21, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView. (nih.gov)
  • This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
  • OfGT3/42/46 were mainly localized in the nuclei and exhibited no transcriptional activities based on the analysis of the subcellular localization and transcriptional activity assay. (biomedcentral.com)
  • In this study, 15 turbot MAPKs (SmMAPKs) were identified throughout the whole genome, and their basic chemical and physical properties and subcellular localization were illustrated. (figshare.com)
  • The subcellular localization of EZH2 protein was predicted by using different predictors (CELLO, Euk-mPLoc, WoLF PSORT, and TargetP). (hindawi.com)
  • Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. (nature.com)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • Subsequent antisense oligonucleotide-knockdown transcriptomic profiling of two LEC- and two BEC-specific lncRNAs identifies LETR1 as a critical gatekeeper of the global LEC transcriptome. (nature.com)
  • The aim of this mini-review is to discuss emerging epigenetic roles of long non-coding RNAs (lncRNAs) in Acute Myeloid Leukemia (AML). (frontiersin.org)
  • In particular, there are incremental examples of the participation of the distinct class of long non-coding RNAs (lncRNAs) in AML epigenetic regulation, some of which have translational potential. (frontiersin.org)
  • Purpose: GG-H whole transcriptome array analysis suggested involvement of PININ (PNN) in the alternative splicing of multiple long non-coding RNAs (lncRNAs). (molvis.org)
  • Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
  • Conclusions: The data suggest PNN's role in the alternative splicing of a specific subset of lncRNAs might have a significant impact on the corneal epithelium. (molvis.org)
  • Therefore, this complex regulatory network requires precise control of gene expression patterns at both transcriptional and post-transcriptional levels in order to ensure proper maturation, differentiation, and formation of blood and lymphatic vessels. (nature.com)
  • Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
  • Alternative splicing of pre-mRNA is a post-transcriptional modification essential for the regulation of gene expression and function. (biomedcentral.com)
  • These participate in transcriptional/translational feedback loops and many homologous clock-components in the fruit fly Drosophila are also expressed in mammalian clock tissues with circadian rhythms. (bioone.org)
  • Circular RNAs (circRNAs) are a new member of noncoding RNAs (ncRNAs) that have recently been described as key regulators of gene expression. (biomedcentral.com)
  • Circular RNAs (circRNAs), a new member of noncoding RNAs (ncRNAs), have attracted great attentions for their closed continuous loop structure and potential value in clinical work [ 9 , 10 ]. (biomedcentral.com)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
  • Floral inducers including FLOWERING LOCUS T (FT), FD, SUPPRESSOR OF CONSTANS 1 (SOC1), and LFEAY (LFY) activate the transcription of the majority of genes floral genes, promoting Arabidopsis bolting and flowering ( Johansson and Staiger, 2015 ). (frontiersin.org)
  • Degenerate oligonucleotide primer sets derived from the highly conserved carboxyl-terminal region of the BMP family were designed and used in reverse transcription-polymerase chain reactions with poly(A) + RNA from articular cartilage as template to determine which BMPs are produced by chondrocytes. (elsevierpure.com)
  • Dirk Schübeler has done important work in epigenomics and regulatory genomics, in particular with the dynamics of genome-wide methylation patterns, their role in gene regulation, and the interplay between chromatin state and the actions of transcription factors. (unibas.ch)
  • It has also been demonstrated that STAT1 (Signal Transducer And Activator Of Transcription 1), a transcription factor (TF) crucially involved in the IFNγ pathway, binds consensus sequences that, in humans, are located with a high frequency in the LTRs (Long Terminal Repeats) of the MER41 family of primate-specific HERVs (Human Endogenous Retrovirus). (biorxiv.org)
  • Interestingly, ID-associated genes exhibit promoter-localized MER41 LTRs that harbor TF binding sites (TFBSs) for not only STAT1 but also other immune TFs such as, in particular, NFKB1 (Nuclear Factor Kappa B Subunit 1) and STAT3 (Signal Transducer And Activator Of Transcription 3). (biorxiv.org)
  • The HOX genes encode a family of homeodomain-containing transcription factors that play important roles in the early embryo, including the establishment of cell and tissue identity, and the regulation of cell proliferation, differentiation, and survival [ 12 ]. (biomedcentral.com)
  • Sustained expression of Hnf6 also results in downregulation of the β-cell-specific transcription factor MafA and a decrease in total pancreatic insulin. (diabetesjournals.org)
  • For many years, the modulation of gene expression was thought to be restricted to, or at least dominated by, the control of transcription. (biologists.com)
  • The authors of the present study previously demonstrated that ectopically induced transcription factors, Octamer-binding transcription factor 4 (OCT-4), sex-determining region Y-box 2 (SOX2), and Kruppel-like factor 4 ( KLF-4) (collectively known as OSK factors) among mammals can reverse aging by restoring youthful patterns of deoxyribonucleic acid (DNA) methylation, transcriptomic profiles, and tissue functioning without cell identity loss. (news-medical.net)
  • TMEM211 RNA Compendium of normal tissues in Canis familiaris. (wikipedia.org)
  • The abundant expression of 9E3 in normal tissues indicates that the product of this gene plays a normal physiological role in tissues growing by cell division, perhaps as a growth regulator. (silverchair.com)
  • The peptide hormone insulin, produced by pancreatic β-cells, is critical for maintaining normal blood glucose levels and acts to regulate cellular metabolism and growth by facilitating glucose uptake by peripheral tissues. (diabetesjournals.org)
  • An RNA-Seq experiment was performed using field grown cotton plants subjected to different water supplies (well-watered and naturally rain fed) to identify differentially expressed transcripts under water-deficit stress. (usda.gov)
  • A total of 1,530 transcripts were differentially expressed between well-watered and water-deficit stressed root tissues, in patterns confirming the accuracy of this technique for future studies in cotton genomics. (usda.gov)
  • Additionally, putative sequence based genome localization of differentially expressed transcripts has detected A2 genome specific gene expression under water-deficit stress. (usda.gov)
  • We reveal that widely used splice-aware aligners, such as STAR and HISAT2, can introduce erroneous spliced alignments between repeated sequences, leading to the inclusion of falsely spliced transcripts in RNA-seq experiments. (bvsalud.org)
  • In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
  • Although the FPPE samples yielded fewer transcripts and genes compared to FF, there was a high agreement in gene expression between paired anatomical areas for FFPE and FF samples. (kth.se)
  • Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. (stanford.edu)
  • Therefore, a clearer understanding capsid expression was analyzed by immunofluorescent assay of WNV is necessary in order to identify new strategies to treat in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag or prevent this viral infection (3). (cdc.gov)
  • In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)
  • The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
  • Prolonged expression of Lin28B delays prosensory cell cycle exit and prevents hair cell differentiation, suggesting that it functions to increase hair cell production [ 2 ] . (encyclopedia.pub)
  • APA naturally occurs during development and cellular differentiation, with around 70% of human genes displaying APA in particular tissues and cell types. (stanford.edu)
  • In this review, we summarize current understanding of how APA is regulated during development and cellular differentiation and how the resulting change in 3'UTR content affects multiple aspects of gene expression. (stanford.edu)
  • No relationship was observed between the expression pattern of β-catenin and cyclin D1 and age, sex, tumor size, stage, differentiation grade, lymph node metastasis, response to chemotherapy, or survival. (biomedcentral.com)
  • The response of cells to environmental signals, as well as their differentiation, death or malignant transformation, involves changes in gene expression. (biologists.com)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • the gene expression profile during B lymphoid differentiation. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • Human genetic variants that introduce an AG into the intronic region between the branchpoint (BP) and the canonical splice acceptor site (ACC) of protein-coding genes can disrupt pre-mRNA splicing. (bvsalud.org)
  • AGAIN identified 350 AG-gain variants from the Human Gene Mutation Database, all of which alter splicing and cause disease. (bvsalud.org)
  • We further showed that AG-gain variants inside the high-risk region led to misspliced products, while those outside the region did not, by two case studies in genes STAT1 and IRF7. (bvsalud.org)
  • To date, a lot of EZH2 variants have been found in various cell and tissue types [ 18 - 20 ]. (hindawi.com)
  • Moreover, none of the EZH2 transcript variants have yet been identified in porcine fetal tissues. (hindawi.com)
  • Interestingly, a mouse ortholog of HAS2-AS1, Has2as, clearly exhibited a differential splicing pattern among three major splice variants in the Pnn-deficient mouse cornea. (molvis.org)
  • Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)
  • Breast cancer risk is influenced by rare coding variants in susceptibility genes, such as BRCA1, and many common, mostly non-coding variants. (cancerindex.org)
  • During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
  • Furthermore, MJ significantly increased the expression of BCRC-3, resulting in an obvious up-regulation of p27. (biomedcentral.com)
  • Molecular mechanisms of gene regulation and/or heritable changes in gene expression caused by mechanisms other than changes in DNA sequence. (ashg.org)
  • The physiological properties of most organisms, from cyanobacteria to human, display a circadian (Latin circa dies , or 'about a day') pattern of activity, which is regulated by an endogenous circadian clock. (bioone.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • Knockouts or loss of function mutations to these family members cause complete or partial deafness in humans and mice that is then inherited in an autosomal recessive pattern. (wikipedia.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Considering the spatial proximity of cells within a tissue sample alongside differential expression analysis offers a more comprehensive picture of disease development, key driver mutations, and possible therapeutic targets. (biochain.com)
  • In this study, we investigated the expression pattern of β-catenin and cyclin D1 using immunohistochemistry and searched for mutations in exon 3 of the β-catenin gene and Axin gene in esophageal squamous cell carcinoma. (biomedcentral.com)
  • Mutations in β-catenin or axin genes are not responsible for this abnormal localization of β-catenin. (biomedcentral.com)
  • This gene plays a role in craniofacial and cognitive development and mutations have been associated with Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. (nih.gov)
  • Mutations in splicing elements, for example, have been found in genes such as LKB1 , KIT , CDH17 , KLF6 and BRCA1 , and changes in trans-acting regulators can affect the expression of genes such as Ron , RAC1 and CD44 . (biologists.com)
  • Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
  • [ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
  • Our work constitutes the first application of the newly published diploid D5 Gossypium raimondii sequence in the study of tetraploid AD1 upland cotton RNA-seq transcriptome analysis. (usda.gov)
  • Here, we identified the coding sequence of the EZH2 gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). (hindawi.com)
  • We present single cell RNA sequence data from 1.3m peripheral blood mononuclear cells from 982 healthy human subjects. (iscb.org)
  • The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
  • Not only do choroid plexus tumors and ependymomas (including the various histologic subtypes) clearly recapitulate specific cell types found at various stages in this ontologic sequence, so too do a variety of other uncommon and/or recently recognized entities. (medscape.com)
  • Additionally, a computational pipeline was used to classify the putative genome localization of genes identified by the RNA sequencing evaluation. (usda.gov)
  • Taken together, these data identify Nanos as primordial genes with highly conserved functions for both, the migration of the germinal cells and their maintenance in adults. (nature.com)
  • The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
  • GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
  • In Drosophila males, meiotic prophase lasts 3.5 days, during which spermatocytes upregulate of over 1800 genes and grow 25-fold. (stanford.edu)
  • We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
  • Localization of 9E3/CEF-4 in avian tissues: expression is absent in Rous sarcoma virus-induced tumors but is stimulated by injury. (silverchair.com)
  • Similar elevation of mRNA occurs immediately adjacent to RSV tumors but, surprisingly, the tumor tissue itself shows no detectable levels of this message. (silverchair.com)
  • Cells explanted from the tumors and grown in culture also show no expression of 9E3, in marked contrast to the very high level found in similarly cultured RSV-transformed CEFs. (silverchair.com)
  • However, the expression of the gene in the periphery of tumors leaves open the possibility that 9E3 may still be involved in RSV carcinogenesis. (silverchair.com)
  • Furthermore, we found homogenous and recurrent iCNV profiles in the high-grade tumors of relapse patients and identified expression of gene SPP1 in the tumor stroma as a potential prognostic mRNA marker in pediatric brain tumor relapse patients. (kth.se)
  • Cancer biomarkers originating from tumors can represent the molecular status of the tumor or its metastases which release them directly into body fluids or indirectly due to disruption of tumor/metastatic tissue. (scientificarchives.com)
  • In addition, OR expression pattern in various cancers were analyzed based on RNA-sequencing data reported in the Cancer Genome Atlas (TCGA), and the associations between patient survival outcomes and OR levels were analyzed to determine the clinical relevance and significances of OR expression in tumors. (bmbreports.org)
  • Since DGASTs are rare tumors, samples in this study were limited, and adjacent normal tissue was not available. (biochain.com)
  • RNA sequencing followed by differential expression analysis was used to identify genes with high expression in DGASTs vs. other GI tumors. (biochain.com)
  • This was possible by comparing expression patterns in stromal cells surrounding the tumors with cells of similar tissues to identify unique patterns. (biochain.com)
  • Mutational analyses of the exon3 of the β-catenin gene and Axin gene were performed on tumors with nuclear β-catenin expression. (biomedcentral.com)
  • Over the past few years, emerging numerous bioinformatic tools have been developed to identify candidate disease-causing genes [ 4 ], including microRNA (miRNA) genes. (hindawi.com)
  • MicroRNA-34a gene (MIR-34A) that is located on chromosome 1p36 belongs to one of evolutionary-conserved miRNA families (MIR-34 family) that consists of three members: MIR-34A, MIR-34B, and MIR-34C [ 10 ]. (hindawi.com)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • (12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)
  • It is possible that Lin28B directly promotes mRNA translation of mTOR pathway genes or relieves let7 -mediated repression of their translation [ 4 ] . (encyclopedia.pub)
  • APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
  • Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • We report that the WNV capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
  • Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
  • We showed that the method is capable of measuring high RNA quality in tissue areas of both high and low cell density and that the spatial RNA integrity patterns are reflected in spatial transcriptomics data. (kth.se)
  • The mitogen-activated protein kinase (MAPK) gene family performs crucial roles in cell division, migration, development, apoptosis, inflammatory response, and abiotic and biotic stress responses. (figshare.com)
  • The term "tumor" as used herein, refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues. (justia.com)
  • The term "cancer" is not limited to any stage, grade, histomorphological feature, aggressivity, or malignancy of an affected tissue or cell aggregation. (justia.com)
  • For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs), and 62,305 trans-eQTL, with the majority showing cell type specific effects on gene expression. (iscb.org)
  • thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
  • Other factors that contribute to a poor prognosis are tumour cell migration into the surrounding tissue, immune evasion [ 8 ], and evasion of cell death induced by radiation and chemotherapy through the activation of anti-apoptotic resistance pathways and upregulation of DNA repair systems. (biomedcentral.com)
  • Malentacchi F, Sorbi F, Cipriani N, Sgromo C, Antonuzzo L, Pillozzi S. Circulating Cell-Free RNA: A New Perspective for Endometrial Cancer. (scientificarchives.com)
  • We now define the mechanism whereby maintenance of Hnf6 expression postnatally leads to β-cell dysfunction. (diabetesjournals.org)
  • These results suggest that downregulation of Hnf6 expression in β-cells during development is essential to achieve a mature, glucose-responsive β-cell. (diabetesjournals.org)
  • Using time-lapse microscopy, we have identified the emergence of this random pattern at the third to fourth cell cycle, when cells started to intermingle. (bioone.org)
  • Present study was designed to establish a causal connection between changes in the cell-cell junction protein expression at the blood-testis barrier and alterations in the adult rat testis histology following an anti-androgen flutamide exposure. (biomedcentral.com)
  • Previous work showed that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. (stanford.edu)
  • A fraction of esophageal squamous cell carcinomas have abnormal nuclear accumulation of β-catenin accompanied with increased cyclin D1 expression. (biomedcentral.com)
  • According to the Jacob-Monod-Lwoff paradigm, established in bacteria and logically extended to eukaryotes, what made a cell a cell was the combinatorial turning on and off of genes. (biologists.com)
  • BCRC-3 was lowly expressed in bladder cancer tissues and cell lines. (biomedcentral.com)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • Microglia cells have been implicated to play an important role in the tumor microenvironment, and we found spatial co-localization of microglia and epithelial-to-mesenchymal transition (EMT) signatures in our patient cohort. (kth.se)
  • There are numerous examples of ocular surface diseases in which the corneal-specific epithelial qualities are not maintained and significant anterior eye physiologic perturbations occur resulting in dramatic loss of vision. (molvis.org)
  • In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
  • Consequently, it lays a foundation for further functional characterizations of these genes. (biomedcentral.com)
  • Using functional imaging and behavioral analysis, loss of Tao-induced ectopic synapses with inappropriate partner neurons were shown to be functional and altered behavioral responses in a connection-specific manner. (sdbonline.org)
  • In endometrial cancer only some of these cf-RNAs have been investigated, no studies have been performed on snoRNAs, piRNAs, snRNAs and tRNA, the most analyzed one is represented by cf-miRNA in plasma. (scientificarchives.com)
  • These findings open an immediate use of selected cf-miRNA as biomarker in liquid biopsy of EC and suggest further investigation of the other cf-RNAs to implement the knowledge in this fascinating field. (scientificarchives.com)
  • Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
  • The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • Differential expression patterns of specific candidate genes associated with water deficit stress were confirmed using Real time quantitative polymerase chain reaction (RT-qPCR). (usda.gov)
  • 5) qPCR for confirmation of differential gene expression. (biochain.com)
  • The platform was then applied to the analysis of differential splice forms in lung cancer samples compared to matched normal lung tissue. (biomedcentral.com)
  • These large-scale high-throughput analytical methods have been applied to the identification of differential splicing events in cancer tissues [ 8 ]. (biomedcentral.com)
  • In a normal lymph node that displays both distinct gene expression patterns and histological landmarks, we observed a neutral iCNV profile. (kth.se)
  • Expression patterns of MAPKs in distinct tissues and under diverse abiotic and biotic stresses were examined using the published available RNA-seq data sets. (figshare.com)
  • As one of the two distinct complexes, namely, Polycomb Repressive Complex 1 (PRC1) and PRC2, PRC2 mediates gene silencing by modulating chromatin structure [ 2 ]. (hindawi.com)
  • This review describes current knowledge about the expression of distinct ORs in cancers, as well as the canonical and non-canonical signaling pathways induced by these ORs. (bmbreports.org)
  • Fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
  • Here, we present evidence that the promoter regions of intellectual disability-associated genes are uniquely enriched in LTR sequences of the MER41 HERVs. (biorxiv.org)
  • Of note, functionally-relevant differences between humans and chimpanzees are observed regarding the 3 main components of this pathway: i) the protein sequences of immunes TFs binding MER41 LTRs, ii) the insertion sites of MER41 LTRs in the promoter regions of ID-associated genes and iii) the protein sequences of the targeted ID-associated genes. (biorxiv.org)
  • The algorithm used to distinguish changes in expression from changes in splicing was calibrated using internal non-spliced control sequences. (biomedcentral.com)
  • Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
  • The blood and lymphatic vascular systems are essential for the efficient transport of oxygen, nutrients, signaling molecules, and leukocytes to and from peripheral tissues, the removal of waste products, and the preservation of fluid homeostasis. (nature.com)
  • The RNA-seq data suggested that the OfGT s have specific expression patterns in different tissues, and some were induced by salt stress. (biomedcentral.com)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • These data will facilitate efforts to understand the complex responses governing transcriptomic regulatory mechanisms and to identify candidate genes that may benefit applied plant breeding programs. (usda.gov)
  • We illustrate where such mechanisms of pattern formation arise in biological systems from cellular to tissue scales, with an emphasis on morphogenesis. (mpi-cbg.de)
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
  • Blencowe's research team studies the mechanisms by which genes are regulated and coordinated to provide critical functions in mammalian cells. (unibas.ch)
  • This review describes OR expression in various types of cancer and the association of these receptors with various types of signaling mechanisms. (bmbreports.org)
  • Empirical and theoretical research on the patterns and determinants of genetic variation within and between populations, especially as they relate to major mechanisms of evolution and their effect on phenotypes. (ashg.org)
  • Northern analyses showed that both genes are predominantly expressed in cartilaginous tissues. (elsevierpure.com)
  • They play major roles in the tissue- and stage-specific expression of protein isoforms as well as in the maintenance of protein homeostasis. (encyclopedia.pub)
  • In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
  • Genome-wide association analysis implicates dysregulation of immunity genes in chronic lymphocytic leukaemia. (cancerindex.org)
  • HOX gene dysregulation is now a widely recognised feature of many malignancies. (biomedcentral.com)
  • In this study we have focused on HOX gene dysregulation in GBM as a potential therapeutic target in a disease with high unmet need. (biomedcentral.com)
  • We show significant dysregulation of these developmentally crucial genes and specifically that HOX genes A9, A10, C4 and D9 are strong candidates for biomarkers and treatment targets for GBM and GBM cancer stem cells. (biomedcentral.com)
  • In mice, the suppression of Nanos3 expression in PGC resulted in the complete loss of germ cells in both sexes 9 . (nature.com)
  • A role of this gene in Germinal Stem Cells maintenance may be evolutionarily conserved as Nanos2 is specifically expressed both in oogonia and in spermatogonia of adult medaka 11 and in a subpopulation of undifferentiated A spermatogonia in juvenile and spermiating male trout 12 . (nature.com)
  • These results show that there are intrinsic differences between transformed embryonic cells in tissue culture and RSV target cells in the hatched chick. (silverchair.com)
  • The research presented in this thesis focuses on developing and applying new tools for interrogating cells and tissues. (kth.se)
  • In Paper I , we describe a protocol for transcript profiling of single cells, capable of measuring the relative expression levels for genes of interest. (kth.se)
  • Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)
  • Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
  • One particular gene of interest ( NKX6.3 ) was found by IHC and qPCR to be expressed in DGAST cells but not in normal tissue - suggesting it plays a role in tumorigenesis or precursor events in the tissue prior to tumor formation. (biochain.com)
  • Although widespread, the role of APA in the biology of cells, tissues, and organisms has been controversial. (stanford.edu)
  • The study assessed the genetic expression patterns of chemically treated cells compared to aged human-origin cells and OSK(MYC)-induced murine and human iPSC. (news-medical.net)
  • The researchers discovered that OSK expression in cells, such as murine and human fibroblasts, may significantly repair the epigenetic environment and patterns of gene expression of aged cells. (news-medical.net)
  • Proliferation of BC cells was suppressed by ectopic expression of BCRC-3 in vitro and in vivo. (biomedcentral.com)
  • [ 7 ] In addition, recent gene expression profiling studies support the concept that radial glial cells from different neuroanatomic sites may be predisposed to acquiring particular genetic aberrations that result in ependymomas with site-specific genetic signatures and biologic potential. (medscape.com)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Loss of epigenetic information is a characteristic of cellular aging in eukaryotes, resulting in changes in gene expression, loss of cellular identity, mitochondrial malfunction, inflammation, and cellular senescence, which contribute to aging and age-related illnesses. (news-medical.net)
  • The system was designed to be efficient, allowing for high-throughput analysis of cellular health and youthful gene expression patterns. (news-medical.net)
  • We use Arabidopsis for forward genetic screens to identify genes important in the response to the wound-induced C6-volatile E -2-hexenal. (uva.nl)
  • We also aim to identify genes that are specifically regulated by E -2-hexenal using a transcriptomics approach. (uva.nl)
  • Since identification of EZH2 in the research of protooncogene product Vav [ 3 ], studies have shown that EZH2 is highly expressed in tumorigenesis, which regulates the expression of tumor suppressor genes, such as in breast cancer, prostate cancer, and lung cancer [ 4 - 6 ]. (hindawi.com)
  • In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)
  • We performed AGAIN on our exome/genomes database of patients with severe infectious diseases but without known genetic etiology and identified a private homozygous intronic AG-gain variant in the antimycobacterial gene SPPL2A in a patient with mycobacterial disease. (bvsalud.org)
  • The discovery of introns and the process of splicing, which eliminates them from the precursor mRNA, introduced an exciting but also perturbing factor in our simplified conceptions of the flow of gene expression in eukaryotes. (biologists.com)
  • The avian gene 9E3/CEF-4, a member of the superfamily of genes that includes KC and gro, is expressed abundantly in exponentially growing cultures of chick embryo fibroblasts (CEFs) and at high levels in CEFs transformed with Rous sarcoma virus (RSV). (silverchair.com)
  • In the early embryonic development, abnormal expression of EZH2 impaired embryo growth and pluripotency maintenance [ 7 , 8 ]. (hindawi.com)
  • The results implied that the OfGT genes were related to abiotic and hormonal stress responses in O. fragrans , and that the OfGT3/42/46 genes in particular might play crucial roles in responses to salt stress. (biomedcentral.com)
  • The results imply that, in addition to PAL, other lignin-specific enzymes and/or tissue structural changes may have more significant roles in tissue toughening of broccoli branchlets. (scialert.net)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • Four (8%) esophageal cancer tissues showed high nuclear β-catenin staining. (biomedcentral.com)
  • Immunofluorescence was performed to assess the expression of pluripotency-related genes following all cocktail treatments. (news-medical.net)
  • DSP enables "visualization" of spatial context in samples that is otherwise missed by bulk RNA sequencing. (biochain.com)
  • Ribonucleic acid (RNA) sequencing (to analyze transcriptomic profiles), signature association, transcriptomic clock, and iPSC profiling analyses were performed. (news-medical.net)
  • Several floral integrator genes including LsCO , LsFT , and LsLFY were co-expressed with LsARF3 in the overexpression and knockout lettuce plants. (frontiersin.org)
  • Mechanistically, overexpression of BCRC-3 induced the expression of cyclin-dependent kinase inhibitor 1B (p27). (biomedcentral.com)
  • Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. (sdbonline.org)
  • In contrast, we found huge variabilities investigating several malign tissue types ranging from homogenous (pediatric medulloblastoma) to highly variable genomes (ductal breast cancer and glioblastoma). (kth.se)
  • Strikingly, we also observed similar iCNV profiles in both tumor and benign tissue areas from prostate and skin cancer. (kth.se)
  • The present invention relates to methods, kits and systems for predicting the benefit from inclusion of taxane in a chemotherapy regimen based on the measurements of gene expression levels in tumor samples of breast cancer patients. (justia.com)
  • Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)
  • These analyses of the relationships between patient survival and expression patterns obtained from an open mRNA database in cancer patients indicate that ORs may be cancer biomarkers and therapeutic targets. (bmbreports.org)
  • We had analyzed circRNA high-throughout sequencing from human tissues and determined bladder cancer related circRNA-3 (BCRC-3, GenBank: KU921434.1) as a new candidate circRNA derived from PSMD1 gene. (biomedcentral.com)
  • This gene is associated to a phenotype (DumPY : shorter than wild-type). (nih.gov)
  • We have established a role for jasmonate (JA) in the production of volatile terpenoids in tomato upon herbivory and discovered that JA induces the expression of salicylic acid (SA) methyl transferase that converts SA to the volatile methyl salicylate, an volatile crucial in attracting predatory mites as shown by silencing SAMT in tomato. (uva.nl)
  • Beyond the variety of molecular implementations of such patterns, we emphasize the unitary principles associated with them, across scales in space and time, within a general mechanochemical framework. (mpi-cbg.de)
  • Ex is required for apical localization of Schip1 in imaginal discs . (sdbonline.org)
  • Mathematical frameworks have been devised to account for the self-organization of biological patterns, most famously the Turing framework. (mpi-cbg.de)
  • Like intercellular RNAs, circulating RNAs participate in numerous biological process and express aberrantly under abnormal or pathological status. (scientificarchives.com)
  • The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining. (hindawi.com)
  • This gene encodes a member of the heterogeneous family of basic, proline-rich, human salivary glycoproteins. (cancerindex.org)
  • A human neonate will imitate a facial expression, such as an outstuck tongue. (amacad.org)
  • However, a growing body of evidence suggests that recruitment, stability, and function of epigenetic factors can be mediated by non-protein-coding RNAs. (frontiersin.org)