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  • contrast
  • In contrast to DSP fixation, DST fixation followed by cleavage with sodium metaperiodate, although effective, results in side reactions that prevent effective protein extraction and interfere with protein identification. (eur.nl)
  • In contrast, tissues fixed in the phosphate buffered saline (PBS) buffer results in lumen and cell morphologies that are consistent with live imaging. (biomedcentral.com)
  • antigen retrieval
  • Recent years have seen the use of different combinations of high temperature and solubilizing agents (usually derived from antigen retrieval techniques) to unravel formaldehyde-fixed paraffin-embedded tissue proteomes. (eur.nl)
  • Distribution and intensity of immunostaining of CAM105 in cryosections of the PFA-fixed rat kidney cortex tissue that underwent various antigen retrieval protocols listed in the section 2.3. (intechopen.com)
  • Relative efficiency of various antigen retrieval protocols B-H in unmasking hidden epitopes in cryosections of the PFA-fixed tissues, as observed in the present study. (intechopen.com)
  • primary
  • Among different stages of tissue preparation, both in biopsy and autopsy samples, two primary stages namely tissue fixation and dehydration are essential and if they are well done, the rest of the process will be easy.One way to facilitate these stages is using an appropriate tissue cassette. (medpip.com)
  • primary walls predominate in young, dividing, and growing cells, whereas secondary walls are characteristic of the thickened walls of woody tissues. (plantphysiol.org)
  • Processing
  • The protocols are for tissue sample processing for subsequent light microscopy with H&E staining, immunocytochemistry or in situ hybridization. (upenn.edu)
  • P.I.P. tissue processing cassette is disposable, made of high-density Acetal polymer and apart from resistance to the common solvents in histology, will keep the sample totally immersed in the fluids. (medpip.com)
  • This instrument is designed for all the stages of processing, embedding, sectioning and storing tissue samples and it can be used from the beginning until the end. (medpip.com)
  • protein
  • However, to achieve protein extraction yields similar to those of fresh-frozen tissue, high-temperature heating is necessary. (eur.nl)
  • Such harsh extraction conditions can affect sensitive amino acids and post-translational modifications, resulting in the loss of important information, while still not resulting in protein yields comparable to those of fresh-frozen tissue. (eur.nl)
  • Herein, the objective is to evaluate cleavable protein crosslinkers as fixatives that allow tissue preservation and efficient protein extraction from fixed tissue for MS proteomics under mild conditions. (eur.nl)
  • In addition, cleavage of DSP improves protein recovery from fixed tissue by a factor of 18 and increases the number of identified proteins by approximately 20 % under mild extraction conditions compared with those of formaldehyde-fixed paraffin-embedded tissue. (eur.nl)
  • Protein crosslinkers that can be cleaved under mild conditions and result in defined modifications, such as DSP, are thus viable alternatives to formaldehyde as tissue fixatives to facilitate protein analysis from paraffin-embedded, fixed tissue. (eur.nl)
  • results
  • This webinar will explore fixation in a historical sense, explore fixative types and chemistries, and compare results of poorly fixed tissues with well-fixed tissues. (nsh.org)
  • central
  • With the emergence of clinically relevant biomarkers, there is the renewed focus on standardization of fixation and other pre-analytical variables: fixation has again become a central topic. (nsh.org)
  • Blood
  • Arteries display highly directional growth and remodeling that are specific to increases in the mechanical loads imposed on them by blood pressure, blood flow, and lengthwise tensile forces that are transmitted from the tissues to which they are attached. (ahajournals.org)
  • days
  • While filling the pouch every few days the tissue expands and the filling material if it is bioactive start to function. (google.co.uk)
  • cells
  • We suggest that PIPES and HEPES buffers should be utilized with caution for fixation when examining the interplay between cells and their extracellular environment, especially in Drosophila pupal and adult retina research. (biomedcentral.com)
  • These findings indicate that influences of mechanical force can modulate the manner in which mitosis of vascular cells contributes to reorganization of arterial wall tissue. (ahajournals.org)
  • We hypothesized that mitosis of vascular cells induced by mechanical forces is polarized so that delivery of daughter cells occurs in a direction that is specific for the force that is applied to the tissue. (ahajournals.org)
  • 9 Endothelial cells at 90% to 100% confluence were cultured in sterile tissue culture dishes (Falcon, VWR) in Medium199 plus 10% FBS (Invitrogen), 1% amphotericin B (Fungizone), and 1% penicillin/streptomycin. (ahajournals.org)
  • A small but growing number of monoclonal antibodies against plant cell wall polysaccharides and glycoproteins have been used to determine the localizations of these macromolecules within plant cells and tissues ( Knox, 1997 ). (plantphysiol.org)