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  • fatty
  • However, a mismatch between the rate of fatty acid uptake and the rate of fatty acid oxidation in the damaged tissue could lead to an imbalance in cytosolic fatty acids that could subsequently lead to an accumulation of toxic signaling lipids such as diacylglycerol (DAG) and/or ceramides. (diabetesjournals.org)
  • Moreover
  • Moreover recombinant PLCbeta1, -gamma1, -gamma2, -delta1, all of which had higher specific activities than boar sperm extracts, were not able to release Ca(2+) in the sea urchin egg homogenate. (ox.ac.uk)
  • liver
  • Also, from what starting amount of liver tissue is this product derived? (abcam.com)
  • I have standardised previous mitochondrial insults by expressing them as being derived from a certain percentage of starting liver tissue. (abcam.com)
  • Hi everyone, this few days I was thinking the benefits to extract DNA from tissues (liver, heart, kidney) rather than other part of our body such as skin, blood etc. (protocol-online.org)
  • specimens
  • 2.When DNA was extracted monthly from muscle specimens which were frozen at -20゚C, a steady decline of the yields of DNA was observed in relation to frozen periods. (nii.ac.jp)
  • 5. When DNA was extracted from the same specimens by using various procedures, DNA of sufficient quantity an quality was obtained by a saturated NaCl method, a liquid nitrogen method and a dry ice method as well as by a usual phenol-chloroform method. (nii.ac.jp)
  • Cryopreserved tissue specimens offer the possibility for sophisticated molecular analysis, but the histomorphology is less well-preserved, and handling is more complicated. (qiagen.com)
  • growth
  • The growth of actinomycetous Frankia strains, 55005, AvcI1, CesI5, CjI82 001, and Cj was stimulated by aqueous extracts of C. cunninghamiana tissue. (springer.com)
  • Aqueous extracts of green cladodes of C. cunninghamiana most stimulated the growth of Casuarina- derived Frankia strains CjI82 001 and 55005. (springer.com)
  • The growth of isolates of soil bacteria Bradyrhizobium japonicum, Arthrobacter globiformis, and Bacillus subtillis and of the soil fungi Penicillium oxalicum and Arthroderma cookiellum was either inhibited or not affected by cladode extracts. (springer.com)
  • Cladode extracts stimulated the growth of the actinomycete Streptomyces albus and the fungus Rhizopus homothallicus . (springer.com)
  • The magnitude (as great as 100%) of the increase in growth caused by tissue extracts for the Casuarina -derived Frankia strains relative to other soil microbes suggests a host-specific enhancement of the microsymbiont. (springer.com)
  • As an attractive source of growth factors, ATE is a promising alternative in the vast field of tissue engineering. (uta.fi)
  • This microarray was used to identify genes that are differentially expressed when bean leaf extracts, pod extracts or apoplastic fluid were added to the growth medium. (biomedcentral.com)
  • Curcumin, the major polyphenol found in turmeric, appears to reduce weight gain in mice and suppress the growth of fat tissue in mice and cell models. (medicalxpress.com)
  • Weight gain is the result of the growth and expansion of fat tissue , which cannot happen unless new blood vessels form, a process known as angiogenesis. (medicalxpress.com)
  • In those mice, we observed a suppression of microvessel density in fat tissue, a sign of less blood vessel growth and thus less expansion of fat. (medicalxpress.com)
  • In general, angiogenesis and an accumulation of lipids in fat cells contribute to fat tissue growth. (medicalxpress.com)
  • Again, based on this data, we have no way of telling whether curcumin could prevent fat tissue growth in humans. (medicalxpress.com)
  • vivo
  • Optical scattering coefficient from ex-vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. (spie.org)
  • Products
  • PCR products prepared from mouse-tail extracts with the IL-1β primer pair were sequenced after purification with the GenElute™ PCR Clean-up Kit (Product Code NA1020) . (sigmaaldrich.com)
  • As shown in Figure 2, PCR products ranging from 1-2 kb were detected on ethidium bromide stained 1% agarose gel from each extract. (sigmaaldrich.com)
  • applications
  • He was the leader for the evaluation of the PAXgene Tissue System within the European collaborative grant project, SPIDIA, and is currently managing work packages in two other grant projects to evaluate the PAXgene Tissue System for NGS applications and use in routine pathology workflows. (qiagen.com)
  • cause
  • Although accumulation of lipid droplets after ischemia is associated with greater infarct size ( 15 ), the precise cause of the tissue damage is not clear. (diabetesjournals.org)
  • Standard
  • Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. (sigmaaldrich.com)
  • For standard PCR, 10 µl of REDExtract-N-Amp™ PCR ReadyMix was combined with forward and reverse primers at 0.4 µM and 4 µl of neutralized tissue extract in a final volume of 20 µl. (sigmaaldrich.com)
  • Single-use aliquots of these DNA standard dilutions were stored at -20 °C. A set of standards was assayed along with the mouse tail extracts. (sigmaaldrich.com)
  • FFPE tissues are still the standard for histomorphological analysis. (qiagen.com)
  • Whole
  • The Ca(2+)-releasing activity was also detectable in sperm from other species and in whole testis extracts. (ox.ac.uk)
  • response
  • phaseolicola NPS3121 in response to tissue extracts from a susceptible Phaseolus vulgarisL. (biomedcentral.com)
  • IL-13Rα2-deficient mice display increased bone marrow macrophage progenitor frequency and decreased tissue macrophage nitric oxide and IL-12 production in response to lipopolysaccharide. (rupress.org)
  • Mouse
  • Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggs. (ox.ac.uk)
  • Therefore, the Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. (sigmaaldrich.com)
  • work
  • The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit. (sigmaaldrich.com)
  • Some tissues are tricky to work with because of inhibitors and contaminants and so it's better to avoid their use (if possible). (protocol-online.org)
  • time
  • These fluorochrome-tissue conjugates were not taken up by cells from lymph nodes excisedfrom the experimental dogs at the time of transplantation or from normal dogs. (sciencemag.org)
  • muscle
  • Much evidence exists to suggest that the improvement in muscle insulin sensitivity may be an indirect consequence of activation of PPAR-γ in fat, a tissue in which the receptor is abundantly expressed. (diabetesjournals.org)
  • release
  • Consistent with a sperm PLC acting on egg PtdIns(4,5)P(2), the ability of sperm extracts to release Ca(2+) was blocked by preincubation with the PLC inhibitor U73122 or by the addition of neomycin to the homogenate. (ox.ac.uk)
  • kits
  • Recently other options such as silica bind and elute kits have become available, but these also require a 5 hour to overnight digestion of the tail before the DNA is extracted. (sigmaaldrich.com)
  • The PAXgene Tissue DNA, RNA, and miRNA Kits are based on proven QIAGEN technologies. (qiagen.com)
  • cells
  • Regional lymph node cells of mongrel dogs which received kidney homografts after bilateral nephrecto-my take up fluorescein-isothiocyanate-labeled extracts of kidney tissue and of peripheral blood leukocytes. (sciencemag.org)
  • appears
  • The mechanism or mechanisms by which curcumin appears to affect fat tissue must be investigated in a randomized, clinical trial involving humans. (medicalxpress.com)
  • volume
  • Adequate vascular formation to the injured area, as well as replacement of the volume loss, is fundamental in soft tissue repair. (uta.fi)
  • For quantitative PCR, 10 µl of Extract-N-Amp™ PCR ReadyMix was combined with forward and reverse primers at 0.4 µM, SYBR ® Green I dye (Product Code S9430) , 0.6 µl of Reference Dye for Quantitative PCR (Product Code R4526) , and 4 ml of tissue extract in a final volume of 20 ml. (sigmaaldrich.com)
  • amount
  • The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. (spie.org)