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Substrate SpecificityTissue DistributionMolecular Sequence DataAmino Acid SequenceKineticsSensitivity and SpecificityBase SequenceCloning, MolecularSequence Homology, Amino AcidOrgan SpecificityBinding SitesModels, MolecularRecombinant ProteinsCatalysisSequence AlignmentEscherichia coliCatalytic DomainHydrolysisDNA, ComplementaryProtein BindingLiverHydrogen-Ion ConcentrationMutagenesis, Site-DirectedSpecies SpecificityIsoenzymesProtein Structure, TertiaryCrystallography, X-RayProtein ConformationStructure-Activity RelationshipRNA, MessengerMolecular WeightAntibody SpecificityChromatography, High Pressure LiquidMutationCell LineElectrophoresis, Polyacrylamide GelBacterial ProteinsDNA PrimersKidneyMolecular StructureCarrier ProteinsPeptidesPhylogenyCattleStereoisomerismAmino AcidsDNAOligopeptidesPhosphorylationRecombinant Fusion ProteinsBrainEnzyme StabilitySerine EndopeptidasesRabbitsBiological TransportMembrane ProteinsAmino Acid SubstitutionProtein Structure, SecondaryAlcohol OxidoreductasesGene ExpressionPolymerase Chain ReactionTemperatureChromatography, GelEndopeptidasesOxidation-ReductionSaccharomyces cerevisiaeAcyltransferasesMembrane Transport ProteinsEnzyme ActivationBlotting, NorthernGene LibraryAmino Acid MotifsCarbohydrate SequenceTransfectionEscherichia coli ProteinsTime FactorsAntibodies, MonoclonalOxidoreductasesHydrolasesOligosaccharidesProtein EngineeringSwineModels, ChemicalProtein IsoformsCell MembranePlasmidsCarbon RadioisotopesModels, BiologicalCOS CellsBlotting, WesternConserved SequenceMass SpectrometryPeptide LibraryBinding, CompetitiveGlycoside HydrolasesRats, Inbred StrainsPeptide FragmentsSpleenGene Expression Regulation, EnzymologicSequence Analysis, DNA
Broad substrate specificitySubcellular localizationDual-Specificity PhosphataseCompound is a substrateEnzymeConnective tissuesDistributed in various tissuesProteinsXenobiotic compoundsDistinctMonoclonalEnzymesInhibitorOATPEndogenousSerineExpressionExtracellular matrixGene structureReactionTransporterUtilizesDifferSulfateCharacterizationUptakeProteinSensitivityFattyMetabolicSpeciesAffinityMonkeyDetectionABSORPTIONSoft-tissueDispositionMetabolismHumansKidneyDrugFoundExplainsCellsImportantHigh-degree
Broad substrate specificity3
Subcellular localization3
  • Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. (nih.gov)
  • Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. (thermofisher.com)
  • These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. (thermofisher.cn)
Dual-Specificity Phosphatase7
  • DUSP9, a Dual-Specificity Phosphatase with a Key Role in Cell Biology and Human Diseases. (nih.gov)
  • Expression of Dual-Specificity Phosphatase 9 in Placenta and Its Relationship with Gestational Diabetes Mellitus. (nih.gov)
  • Find Dual Specificity Phosphatase 3 Antibodies for a variety of species such as anti-Human Dual Specificity Phosphatase 3, anti-Mouse Dual Specificity Phosphatase 3, anti-Rat Dual Specificity Phosphatase 3. (antibodies-online.com)
  • Find Dual Specificity Phosphatase 3 Antibodies validated for a specific application such as WB, ELISA, IHC, IF (cc). (antibodies-online.com)
  • Find Dual Specificity Phosphatase 3 Antibodies with a specific Host. (antibodies-online.com)
  • Find available monoclonal or polyclonal Dual Specificity Phosphatase 3 Antibodies. (antibodies-online.com)
  • Find Dual Specificity Phosphatase 3 Antibodies with a specific conjugate such as Biotin, FITC, HRP. (antibodies-online.com)
Compound is a substrate2
  • Cyprotex's preclinical species hepatic Oatp transporter substrate identification assay determines if your compound is a substrate of key preclinical species transporters. (cyprotex.com)
  • Cyprotex's MAO reaction phenotyping assay identifies if your compound is a substrate for either MAO-A or MAO-B. (cyprotex.com)
Enzyme7
  • Metabolic stability of the MAO substrate, kynuramine over 45 min in the presence of MAO-A and MAO-B expressed enzyme (Each data-point is mean of n=3). (cyprotex.com)
  • sPLA 2 isoforms have unique tissue and cellular distributions, and therefore it is evident that individual sPLA 2 s have distinct enzyme-activity-related biological functions. (ijbs.com)
  • The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. (caslab.com)
  • CYP1A1 is mainly found in extrahepatic tissues while CYP1A2 is the major CYP enzyme in human liver. (xula.edu)
  • Propeptides bind to their cognate enzyme with high specificity. (biomedcentral.com)
  • Little is known, however, about the mechanism by which the propeptide of FhCL3, a cathepsin L peptidase secreted by the infective newly excysted juveniles (NEJs), regulates the inhibition and activation of the mature enzyme before it is secreted into host tissues. (biomedcentral.com)
  • Engineering the substrate specificity of toluene degrading enzyme XylM using biosensor XylS and machine learning. (go.jp)
Connective tissues1
  • Sulfonation is one of the most abundant cellular reactions modifying a wide range of xenobiotics as well as endogenous molecules which regulate important biological processes including blood clotting, formation of connective tissues, and functionality of secreted proteins, hormones, and signaling molecules. (oncotarget.com)
Distributed in various tissues1
  • The SULF1 and SULF2 transcripts were widely distributed in various tissues. (scielo.br)
Proteins1
  • Fibrosis is characterised by an excessive production of extracellular matrix components, and cells in the tissue are constantly interacting with these extracellular matrix proteins. (bmj.com)
Xenobiotic compounds1
  • The UGT substrates include many endogenous and xenobiotic compounds. (aspetjournals.org)
Distinct1
  • sPLA 2 s show distinct substrate specificities in terms of the phospholipid polar headgroups and the fatty acids esterified at the sn- 2 position of the glycerol backbone. (ijbs.com)
Monoclonal1
  • They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. (thermofisher.cn)
Enzymes5
  • The six CerS enzymes have different specificity for acyl-CoA substrates, resulting in the generation of dihydroceramides with differing chain lengths (ranging from C14-C26). (wikipedia.org)
  • These cytosolic enzymes are different in their tissue distributions and substrate specificities. (origene.com)
  • Review of Ligand Specificity Factors for CYP1A Subfamily Enzymes from " by Jayalakshmi Sridhar, Navneet Goyal et al. (xula.edu)
  • A complete understanding of the factors that govern the specificity and potency for the two CYP 1A enzymes is critical to the development of effective inhibitors. (xula.edu)
  • In this review, we perform a thorough analysis of the computational studies that are ligand-based and protein-ligand complex-based to catalog the various factors that govern the specificity/potency toward these two enzymes. (xula.edu)
Inhibitor1
OATP7
  • We aimed to characterize the novel OATP-B with respect to tissue distribution and hepatocellular localization and to compare its substrate specificity with those of OATP-A, OATP-C, and OATP8. (nih.gov)
  • Tissue distribution and hepatocellular localization of OATP-B were analyzed by Northern blotting and immunofluorescence, respectively. (nih.gov)
  • Transport of 16 substrates was measured for each individual human OATP in complementary RNA-injected Xenopus laevis oocytes. (nih.gov)
  • Understand if your compound is an in vitro substrate for the main hepatic Oatp uptake transporter of preclinical species, namely rat Oatp1b2, dog Oatp1b4 or Cynomolgus monkey Oatp1b1. (cyprotex.com)
  • Preclinical species hepatic Oatp uptake transporter substrate identification is within our portfolio of in vitro drug transporter services. (cyprotex.com)
  • The use of in vitro cell test systems that each overexpress the major hepatic Oatp transporter of preclinical species (Oatp1b2, Oatp1b4 or Oatp1b1 for rat, dog or Cynomolgus monkey, respectively) may be useful towards understanding whether a molecule is a substrate of an hepatic active uptake transporter in those species. (cyprotex.com)
  • Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): Phylogenetic analysis and tissue distribution. (irb.hr)
Endogenous2
  • One of the most important human SLC transporters expressed in human liver is OATP1B1 which is responsible for the hepatic uptake and rate-determining elimination of a wide range of endogenous compounds and drugs that are substrates 2 . (cyprotex.com)
  • Typical endogenous substrates are bilirubin and estradiol. (aspetjournals.org)
Serine2
Expression5
  • HFD ± NDEA caused T2DM, neurodegeneration with impairments in brain insulin, insulin receptor, IGF-2 receptor, or insulin receptor substrate gene expression, and reduced expression of tau and choline acetyltransferase (ChAT), which are regulated by insulin and IGF-1. (biomedcentral.com)
  • Species differences in drug transporters with regard to their tissue distribution, expression levels and substrate specificity can be problematic for preclinical cross-species extrapolation of drug disposition (clearance) and DDI potential to human. (cyprotex.com)
  • The tissue-specific expression of AQPs suggests an important role in the development and function of the respective organs. (biomedcentral.com)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation. (geneticsmr.com)
Extracellular matrix1
  • The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. (bmj.com)
Gene structure1
  • Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity. (geneticsmr.com)
Reaction2
  • An unseeded (lane U) reaction was included as a specificity control. (cdc.gov)
  • After each round, reaction products (1 volume) were mixed with fresh substrate (9 volumes) to seed the following round. (cdc.gov)
Transporter1
  • However, several of these transporter-mediated-DDIs do not manifest themselves in increased plasma concentration levels of the affected FQ, so the assessment of FQ concentration levels in peripheral tissues is of interest to detect or predict a potential DDI. (bruker-webdev.de)
Utilizes1
  • This isozyme preferentially utilizes arachidonate as substrate. (nih.gov)
Differ1
Sulfate2
  • The purpose of this study was to clarify the involvement of uremic toxins (indoxyl sulfate (IS), 3-indoleacetic acid, p-cresyl sulfate and kynurenic acid) on CKD-induced adipose tissue inflammation. (mdpi.com)
  • The extreme structural diversity typical of HS species is a result of the variable distribution of these residues as well as of sulfate substituents along the chain. (silverchair.com)
Characterization1
Uptake1
  • The uptake of [ 3 H]vinblastine, a substrate of P-gp, by Caco-2 cells was measured. (aspetjournals.org)
Protein3
  • The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. (nih.gov)
  • Western blots of variant Creutzfeldt-Jakob disease (vJCD) proteinase K-resistant prions (PrP res ) analyzed by protein misfolding cyclic amplification (PMCA) in tissues of clinical and asymptomatic patients. (cdc.gov)
  • PMCA substrate was prepared by using brains from transgenic mice overexpressing the ARQ variant of sheep prion protein. (cdc.gov)
Sensitivity2
  • In addition to its non-invasive nature, the advantages of PET are its high sensitivity (only a few µg of the radiolabelled compound is required) and its high temporal resolution, enabling the detection of concentration changes in tissues within several seconds. (bruker-webdev.de)
  • In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
Fatty1
  • For their sn -2 fatty acid tail specificities, GIB, GIIA and GIIE sPLA 2 s are promiscuous. (ijbs.com)
Metabolic1
Species1
  • We clarified that the species differences in UGT activity evaluated by the CL int or CL max values in liver and duodenum varied according to the substrate. (aspetjournals.org)
Affinity2
  • Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. (joplink.net)
  • Several reviews and reports present the advantages and limitations of the currently available algorithms and software for in silico modelling of the drug dissolution/bioavailability, oral absorption rate/fraction, volume of distribution, path- ways, affinities or rates of metabolism, renal excretion rate as well as affinity for specific transporters [3-20]. (cdc.gov)
Monkey1
Detection1
  • Examination of direct smears for acid fast bacilli is the widely used method for the detection of mycobacteria, but lacks the desired specificity and relatively large number of bacteria is needed for detection. (who.int)
ABSORPTION3
  • Their polar nature restricts movement of FQs across biological membranes, making active and facilitated transport processes the major determinants of FQ absorption, distribution and elimination. (bruker-webdev.de)
  • Even though this approach is conceptually applicable to non-volatile and high molecular weight organics as well, it is more challenging to predict the other PBPK model parameters required for modelling the kinetics of these chemicals (particularly tissue diffusion coefficients, association constants for binding and oral absorption rates). (cdc.gov)
  • The pharmaceutical literature consists of numerous examples of 2-D QSARs, 3-D QSARs and expert systems for modelling the individual components or phases of drug disposition and pharmacokinetics (i.e. absorption, distribution, metabolism and *Corresponding author. (cdc.gov)
Soft-tissue2
  • The ET-D gene was detected mainly in isolates from patients with skin and soft-tissue infections, namely furuncles, abscesses, and finger pulp infections. (medscape.com)
  • In contrast to PET, Magnetic Resonance Imaging (MRI) delivers anatomical information with high spatial resolution and excellent soft-tissue contrast. (bruker-webdev.de)
Disposition1
  • The aim of this study was to evaluate effect of CBD on the disposition of the CES1 substrate methylphenidate (MPH). (inra.fr)
Metabolism2
  • Sphingolipidoses, or disorders of sphingolipid metabolism, have particular impact on neural tissue. (wikipedia.org)
  • Liver and intestine are the important tissues for drug metabolism including glucuronidation. (aspetjournals.org)
Humans1
  • This is difficult to investigate in humans due to the invasive nature of tissue biopsies. (bruker-webdev.de)
Kidney2
  • Adipose tissue inflammation appears to be a risk factor for the progression of chronic kidney disease (CKD), but the effect of CKD on adipose tissue inflammation is poorly understood. (mdpi.com)
  • They are eliminated via the kidneys, leading to high concentration levels in kidney tissue and urine. (bruker-webdev.de)
Drug1
  • Positron Emission Tomography (PET) is a nuclear imaging technique for non-invasive determination of the concentration of a radiolabelled drug in tissues. (bruker-webdev.de)
Found1
  • Additionally, the neuronal type of nAChRs can also be found in non-neuronal tissues such as lymphocytes, platelets, macrophages, myelo- and erythrocyte progenitors, as well as mature erythrocytes [2][3]. (dysona.org)
Explains1
  • The distribution of desmogleins in the epidermis explains the preferential blistering of SSSS in the superficial skin and lack of blistering in mucous membranes. (medscape.com)
Cells1
  • the substrate used for sulfonation reactions in cells. (oncotarget.com)
Important1
  • Despite their importance, limited data is available about AQP distribution and function in the economically important oilseed crop peanut, Arachis hypogea (AABB). (biomedcentral.com)
High-degree1
  • When introducing a security administration system, an essential first step is for an organization to nominate a senior govt who takes direct responsibility for security and who has some high-degree influence on the distribution of funds. (ehd.org)