• In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
  • Hyperglycemia and advanced glycation end products disrupt BBB and promote occludin and claudin-5 protein secretion on extracellular microvesicles. (elisakits.co.uk)
  • The expression and phosphorylation of Hif-1α, VEGF, VEGFR-2, ERK and occludin were measured by Western blot analysis. (researchsquare.com)
  • In addition, the inhibitors of Hif-1α, VEGF, VEGFR-2, and ERK could reduce the effect of TNF-α on occludin expression. (researchsquare.com)
  • Conclusion: TNF-α could decrease the expression of occludin via Hif-1α/VEGF/VEGFR-2/ERK signaling pathway, which was attenuated by propofol. (researchsquare.com)
  • as well as the tight junction protein claudin-1 and occludin. (biotech2012.org)
  • For instance, anti-Claudin 18.2 antibody screening was incorporated with a counter-screen against Claudin 18.1, which is ubiquitously expressed in normal tissue. (genscriptprobio.com)
  • SAN FRANCISCO ― An experimental monoclonal antibody combined with chemotherapy could offer a new standard of care for patients with claudin-18.2+ (CLDN18.2+) / HER2− advanced gastric or gastroesophageal junction (mG/GEJ) adenocarcinoma, say researchers in reporting a phase 3 trial. (medscape.com)
  • A Phase II, Open-label, Multi-centre Study to Evaluate Safety, Tolerability, Efficacy, PK, and Immunogenicity of AZD0901 as Monotherapy and in Combination With Anti-cancer Agents in Participants With Advanced Solid Tumours Expressing Claudin 18.2. (who.int)
  • Scholars@Duke publication: The expression of Claudin-4 in gastric cancer tissue: A single center experience. (duke.edu)
  • Furthermore, the study establishes a new clinically relevant biomarker in gastric cancer called claudin-18 isoform 2 (CLDN18.2). (m3india.in)
  • One reason may be that claudin 18 positivity may be associated with a better prognosis for patients with diffuse gastric cancer, he explained. (medscape.com)
  • Overall, Wang believes that these results validate claudin 18 as a new predictive biomarker for advanced gastric cancer, although he noted that widespread implementation for this indication may be limited, as the assay to determine claudin 18 levels is not widely available. (medscape.com)
  • Immunohistochemistry (IHC) was used for assessing the expression of claudin-4 with a semi-quantitative model. (duke.edu)
  • Claudin-2 expression was examined by immunohistochemistry on tissue microarrays, containing EAC, high grade dysplasia (HGD), low grade dysplasia (LGD), Barrett's esophagus (BE), columnar cell metaplasia (CM), squamous cell carcinoma (SCC), and squamous epithelium (SE) cases. (biomedcentral.com)
  • Cancer tissues were subjected to immunohistochemistry with antibodies against claudin-3, claudin-4, beta-catenin, matrix metalloproteinase (MMP)-1, and MMP-2, as well as endothelial markers of lymphatic vessel endothelial hyaluronan receptor-1 and von Willebrand factor for the objective discrimination between lymphatics and blood vessels. (toho-u.ac.jp)
  • The expression of sealing claudin-1, claudin-3, claudin-4, and claudin-8, and pore-forming claudin-2 in humans and rodents has been evaluated by immunohistochemistry and quantitative polymerase chain reaction. (figshare.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Claudin 2 (CLDN2) in tissue homogenates, cell lysates and other biological fluids. (scalegen.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Claudin 2 (CLDN2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
  • The following product was used in this experiment: ZO-2 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 71-1400, RRID AB_2533976. (thermofisher.com)
  • Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. (labome.cn)
  • Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) - Azide and BSA Free [NBP2-71329] - Claudin-5 labeled pig SC cells visualized by alexa 594. (labome.cn)
  • Anti- Claudin 5 Picoband antibody, RP1043, Western blotting. (labome.cn)
  • This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
  • Claudin-2 increases the calcium permeability of renal epithelial cells upon overexpression. (ku.edu)
  • I found no effect of claudin-3 overexpression on conductance or sodium permeability (PNa), with or without claudin-2 expression. (ku.edu)
  • The results of calcium permeability assays show that calcium transport was reduced with co-expression of claudins-2 and -10a but unchanged with co-expression of claudins-2 and -3. (ku.edu)
  • In order to test the potential contribution of claudin hetero-oligomerization on these permeability properties, I measured permeability of claudin-2/claudin-10a cells with titrated expression of each protein and with blockage of the claudin-2 pore. (ku.edu)
  • Claudin-5 regulates the paracellular permeability in the blood-brain barrier and is the enriched tight junction protein at the BBB. (elisakits.co.uk)
  • Claudin-10a deficiency shifts proximal tubular Cl-permeability to cation selectivity via claudin-2 redistribution. (mpg.de)
  • First, I examined the patterns of claudin expression in the proximal nephron and found that claudins-2 and -10a are expressed throughout the PT, in both convoluted and straight segments. (ku.edu)
  • Furthermore, claudin-2 and claudin-3 expression are found in separate and distinct subpopulations of thin descending limbs. (ku.edu)
  • Then I generated renal epithelial cell lines with inducible expression of PT claudins, claudin-2 and either claudin-3 or claudin-10a. (ku.edu)
  • Importantly, cis-acting expression quantitative trait loci (eQTL) analysis reveals that these same risk variants correlate with reduced claudin-2 mRNA expression in human tissues. (ku.edu)
  • In addition, claudin-2 expression is an important mediator of calcium transport that is associated with kidney stone disease in humans. (ku.edu)
  • The purpose of this investigation was to study the expression of claudin-4 in the various histological subtypes of GC and to evaluate its prognostic value. (duke.edu)
  • RESULTS:66.7% of our cases showed abnormal claudin-4 expression in IHC. (duke.edu)
  • The correlation of claudin-4 tissue expression with patient overall survival survival (OS) or disease-free survival (DFS) was not statistically significant, as well as with age, gender, tumor N stage, grade, TNM stage, positive lymph node ratio or lymphovascular invasion. (duke.edu)
  • Recently, we found that vitamin D receptor (VDR) enhanced Claudin-2 expression in colon and that bile salt receptors VDR and Takeda G-protein coupled receptor5 (TGR5) were highly expressed in esophageal adenocarcinoma (EAC) and precancerous lesions. (biomedcentral.com)
  • Here, we examined the expression of Claudin-2 in EAC and precancerous lesions and its association with VDR and TGR5 expression. (biomedcentral.com)
  • High expression was defined as 2-3 intensity in ≥ 10% of cells. (biomedcentral.com)
  • It was significantly more highly-expressed in EAC, SCC and glandular lesions than in SE and more in EAC than in BE and CM. A significant association was found between Claudin-2 expression and VDR and TGR5 expression. (biomedcentral.com)
  • No significant association was found between expression of Claudin-2 and age, gender, grade, stage, or patients' survival time in EAC and SCC. (biomedcentral.com)
  • We conclude that Claudin-2 expression is significantly associated with bile acid receptors VDR and TGR5 expression. (biomedcentral.com)
  • Claudin-2 expression may be involved at early stages of transformation in inflammatory bowel disease-associated neoplasia [ 23 ]. (biomedcentral.com)
  • Furthermore, SARS-CoV-2 spike (S) protein expression was investigated in cells of the placenta, amnion, and umbilical cord. (news-medical.net)
  • SARS-CoV-2 spike (S) expression was sparse in cortical microtubule-associated protein 2 (MAP2)-positive and HuC/D-positive neurons and the nestin-positive and Hopx (homeodomain-only protein)-positive radial glial, and Sox2 (sex-determining region Y-box 2)-positive ventricular zone and subventricular zone progenitor cells. (news-medical.net)
  • In the cortical regions of the hemorrhagic specimens, blood vessels situated proximal to erythrocyte clusters showed significantly lower claudin-5 expression compared to away from vessels located far from the erythrocyte clusters. (news-medical.net)
  • M M P-2 and -9 expression were nearly absent in all groups. (unesp.br)
  • No MMP-2 or -9 expression was observed in any endometrial tissue sample. (unesp.br)
  • JAM-A Acts via C/EBP-α to Promote Claudin-5 Expression and Enhance Endothelial Barrier Function. (nih.gov)
  • Expression profiles of claudin-6, -10d and - 10e in the euryhaline teleost fish Tetraodon nigroviridis revealed claudin-6 in brain, eye, gill and skin tissue, while claudin-10d and - 10e were found in brain, gill and skin only. (silverchair.com)
  • In patients with inflammatory bowel disease (IBD), disease activity-dependent changes in expression of certain claudins have been noted, thus making certain claudin family members potential therapy targets. (figshare.com)
  • Claudin expression by human mononuclear cells isolated from lamina propria has been confirmed by Western blot and flow cytometry. (figshare.com)
  • The claudin expression pattern in uninflamed and inflamed colon varied between species and murine strains. (figshare.com)
  • In IBD and both animal models, diverse alterations in claudin expression by epithelial and inflammatory cells were recorded. (figshare.com)
  • Dietary N reduction led to a segment specific increase in tissue conductances in the proximal jejunum which might be linked to concomitantly decreased expression of cadherin 17 mRNA. (equine-behaviour.de)
  • Reduced dietary Ca supply resulted in a segment specific increase in claudin 2 and claudin 12 expression and decreased the expression of OCLN which might have been mediated at least in part by calcitriol. (equine-behaviour.de)
  • We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)
  • We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
  • We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. (frontiersin.org)
  • Cross-reactivity occurs when amino acid sequence homology exists between a pathogen and self-tissue proteins ( 1 ). (frontiersin.org)
  • In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)
  • We believe the answer is probable, since some of the systemic disease clinical manifestations of COVID-19 cannot be explained solely by the binding of SARS-CoV-2 spike proteins with cell membranes of tissues that exhibit angiotensin-converting enzyme 2 (ACE2). (frontiersin.org)
  • Objective: The aim of this study was to investigate the malignant potential of endometrial polyps (EP) by assessing the immunoexpressions of both estrogen receptor (ER) and progesterone receptor (PR), Ki-67 cell proliferation index, neovascularization network (endoglin - CD105), cellular adhesion molecules (claudins 3 and 4), and extracellular matrix proteins (MMP-2 and -9) in both EP and endometrioid adenocarcinoma (type I) in comparison with the normal endometrium. (unesp.br)
  • They stained positively for specific cell-cell interaction markers indicative of a functional meningeal tissue, as well as extracellular matrix proteins found in human meninges. (biomedcentral.com)
  • However, overexpression of both claudins-2 and -10a led to a highly conductive cell monolayer with loss of charge selectivity. (ku.edu)
  • The immunoexpressions of ER, PR, Ki-67, CD105, claudins 3 and 4, and MMP-2 and -9 were assessed in paraffin blocks containing sections of the largest polyploid lesion fragment and tissue microarray recipient blocks. (unesp.br)
  • Taken together with our knowledge of claudin-6 and -10 function in other vertebrates, data support the idea that in SW-acclimated T. nigroviridis , these claudins are abundant in gill ionocytes, where they contribute to the formation of a Na + shunt and 'leaky' epithelium, both of which are characteristic of salt-secreting SW fish gills. (silverchair.com)
  • Description: Quantitativesandwich ELISA kit for measuring Human Claudin-2(CLDN2) in samples from serum, plasma, tissue homogenates, cell lysates. (scalegen.com)
  • Human claudin-5 ELISA kit can measure concentrations of CLDN5 (claudin-5, BEC1, CPETRL1, TMVCF1) present is human biological fluids, serum, plasma and tissue homogenate samples. (elisakits.co.uk)
  • For this claudin-5 ELISA kit it is recommended that a standard curve is generated for each assay carried out. (elisakits.co.uk)
  • Tissue mRNA levels for each studied claudin reflected changes within cell lineage and, at the same time, mirrored the ratio between various cell types. (figshare.com)
  • In contrast, claudin-3 is expressed only within the proximal straight tubule (PST). (ku.edu)
  • Next, I tested the hypothesis that deletion of the claudin-2 gene Cldn2 in mice causes nephrocalcinosis similar to human kidney stone disease using micro-computed tomography (micro-CT) and histological analyses. (ku.edu)
  • Description: A competitive ELISA for quantitative measurement of Human Claudin 2(CLDN2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (scalegen.com)
  • Cell Culture Infectivity Assay for Norovirus cally resemble in vivo tissues and organs ( 25 ). (cdc.gov)
  • Claudin-2 forms gated paracellular channels and allows sodium ions and other small positively charged ions to cross between adjacent cells. (biomedcentral.com)
  • [ 2 ] The arachnoid cells have several proposed functions, including acting as a structural barrier with cellular wrapping/ensheathing, acting as a conduit for cerebrospinal fluid (CSF) drainage/absorption into dural sinuses/veins (arachnoid villi), epithelial-like/secretory functions, monocytelike functions, trophic support and byproduct detoxification for glial and neuronal cells, and participation in reactive/reparative processes. (medscape.com)
  • Studies of viral attachment to cells on porous collagen-I coated microcarrier beads under cultured gastrointestinal epithelial cells (Caco-2) using conditions of physiological fluid shear in rotating wall ves- recombinant virus-like particles or infectious noroviruses sel bioreactors. (cdc.gov)
  • This biomarker can be identified using a staining method in tumor biopsies, a procedure to remove cells or tissue from an affected area. (m3india.in)
  • Next, the team examined the counts of cleaved caspase-3+ cells to determine whether the older or recent hemorrhages were related to increased cell deaths within cortical tissues. (news-medical.net)
  • It represents a unique target in that it is restricted to differentiated stomach cells only, and LDN18.2 is absent from all other healthy tissues. (medscape.com)
  • Normally, potentially malignant cells are continuously eliminated by the immune system, but cancer cells can accumulate certain mutations, which allow them to escape these mechanisms [ 2 ]. (nature.com)
  • In skin, claudin-6 was found to co-localize with NKA-ir cells, but claudin-10d and -10e did not. (silverchair.com)
  • To overcome the lack of suitable in vitro models that faithfully recapitulate the intricate three-dimensional architecture, complex cellular interactions, and fluid dynamics within the SAS, we have developed a perfusion bioreactor-based 3D in vitro model using primary human meningothelial cells (MECs) to generate meningeal tissue constructs. (biomedcentral.com)
  • Primary human meningothelial cells (phMECs) were seeded and cultured on collagen scaffolds in a perfusion bioreactor to generate engineered meningeal tissue constructs. (biomedcentral.com)
  • Inflammatory Alveolar Type 2 Cells in Chronic Obstructive Pulmonary Disease: Impairing or Improving Disease Outcome? (lu.se)
  • Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. (wikipedia.org)
  • On the basis of sequence diversity of the lack of suitable tissue culture or animal models, the true capsid gene, noroviruses can be classified into 8 genetic nature of norovirus pathogenesis remains unknown. (cdc.gov)
  • Association of +62 G>A Polymorphism in the Resistin Gene with Type 2 Diabetes Mellitus among Thais: Case-Control Study. (kku.ac.th)
  • Interestingly, neither of these features seemed to be associated with a role for Keratin76 in maintaining structural stability, because cell lysis or tissue blistering was not evident in mice lacking the gene. (plos.org)
  • CONCLUSIONS:Literature stands equivocal about the exact role and prognostic value of claudin-4 and histopathology and tumor invasiveness in patients with GC. (duke.edu)
  • First, does cross-reactivity play a role in the multi-system disorders associated with SARS-CoV-2 infection? (frontiersin.org)
  • The team examined the number of hemorrhagic specimens associated with SARS-CoV-2 infection cases based on data sourced from the https://coronavirus.data.gov.uk/ website. (news-medical.net)
  • Description: tissue homogenates, cell lysates and other biological fluids. (scalegen.com)
  • Claudin-4 was significantly correlated with tumor T stage and with intestinal type classification. (duke.edu)
  • The blood-brain barrier (BBB) and the blood-spinal cord barrier (BSCB) are responsible for controlling the microenvironment within neural tissues in humans. (hindawi.com)
  • Disruption to the blood-brain barrier (BBB) is commonly observed in experimental models of epilepsy and in resected brain tissue from patients with treatment resistant temporal lobe epilepsy. (tcd.ie)
  • Modifying the blood-brain barrier by targeting claudin-5: Safety and risks. (elisakits.co.uk)
  • 2 2 Gaudier E, Hoebler C. Physiological role of mucins in the colonic barrier integrity. (scielo.br)
  • Basel, Switzerland - 13 April 2023, Alentis Therapeutics ("Alentis"), the Claudin-1 (CLDN1) company, is a clinical-stage biotech developing breakthrough treatments for organ fibrosis and CLDN1 positive tumors. (alentis.ch)
  • At the American Association for Cancer Research earlier this year, Synthekine presented data that showed STK-012 induced potent anti-tumor activity while avoiding the toxicities that have hindered the development of IL-2 therapeutics, including vascular leak syndrome (VLS). (biospace.com)
  • Our results further strengthen the need of larger studies to fully elucidate the predictive role of claudin-4 in the natural history of GC. (duke.edu)
  • We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)
  • We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. (bicellscientific.com)
  • Claudin-2 was highly expressed in 77% EAC (86/111), 38% HGD (5/13), 61% LGD (17/28), 46% BE (18/39), 45% CM (29/65), 88% SCC (23/26), and 14% SE (11/76). (biomedcentral.com)
  • Claudin-5 consists of 4 transmembrane domains, a short NH2 terminus, two extracellular loops (ECL), a short intracellular loop and a longer COOH terminus. (elisakits.co.uk)
  • TGF-β1 is a major mediator of airway tissue remodelling during atopic asthma and affects tight junctions (TJs) of airway epithelia. (springer.com)
  • This technique is specific and uses native tissues for functional studies of epithelia. (jove.com)
  • These demonstrations of BBB dysfunction have been confirmed with biofluid biomarker data and observations of postmortem tissue analysis [ 6 , 12 - 17 ]. (hindawi.com)
  • These barriers are fundamental to all neurological processes as they provide the extreme nutritional demands of neural tissue, remove wastes, and maintain immune privileged status. (hindawi.com)
  • Biocompatibility as well as biosafety of butterfly chicken wings for your scientific utilization of tissue-engineered neural grafts. (peptide-solubility.com)
  • As such, it is maintained within a narrow range through the actions of gastrointestinal, bone, kidney, and endocrine tissues. (ku.edu)
  • The study was conducted between July 2020 and mid-April 2022 in the United Kingdom (UK), wherein human fetal tissues aged 9.0 to 21.0 pcw (post-conception weeks) were obtained from the HDBR (human development biology resource). (news-medical.net)
  • Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • This week's $67 million raise will help advance therapies targeting Claudin-1 for liver and kidney disease into the clinic. (biospace.com)
  • Midway between these two, type 2 patients had stones and kidney tissue calcium deposits but adequate kidney function. (uchicago.edu)
  • His Type 1 patients, who simply formed kidney stones, rarely came to autopsy, so he did not have tissues from them. (uchicago.edu)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • Using dilution potentials, you can measure the permselectivity and get an understanding of tight junctions in a tissue. (jove.com)
  • Strikingly, one of the principal protein components of the tight junction, Claudin 1, was mislocalised. (plos.org)
  • Our paper therefore proposes that Keratin76 contributes to normal tight junction function by ensuring that Claudin1 localises appropriately to the structure [ 2 ]. (plos.org)
  • Influence of the application of enemas with oily extract of curcumin ( Curcuma longa ) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream ☆ ☆ Study conducted at Universidade São Francisco (USF), Programa de Pós-Graduação em Ciências da Saúde, Laboratório de Investigação Médica (LIM), Bragança Paulista, SP, Brazil. (scielo.br)
  • To verify if the application of enemas containing oily extracts of curcumin preserves the tissue content of mucins in the glands of the colonic mucosa without fecal stream. (scielo.br)
  • In vitro differentiation of small intestinal epithelium that nausea, vomiting, and watery diarrhea within 12-24 hours approaches physiologic functionality of the in vivo host of exposure and typically remain symptomatic for 1-2 may allow for the development of a pathogenesis model days ( 5 ). (cdc.gov)
  • Thus, our results can establish the potential risk for autoimmunity and multi-system disorders with COVID-19 that may come from cross-reactivity between our own human tissues and this dreaded virus, and thus ensure that the badly-needed vaccines and treatments being developed for it are truly safe to use against this disease. (frontiersin.org)
  • The COVID-19 pandemic has placed an immediate call to action for medical researchers to investigate how SARS-CoV-2 can impact the worldwide human population. (frontiersin.org)
  • SARS-CoV-2 can reportedly infect developing human neurons within cerebral organoids. (news-medical.net)
  • Finding the best clearing approach: Towards 3D wide-scale multimodal imaging of aged human brain tissue. (mpg.de)
  • In the present study, researchers assessed the effects of coronavirus disease 2019 (COVID-19) on fetal brain tissues. (news-medical.net)
  • This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
  • There are three important questions regarding the role of cross-reactivity with SARS-CoV-2. (frontiersin.org)
  • Second, how does cross-reactivity contribute to the pathophysiology of SARS-CoV-2-induced autoimmunity? (frontiersin.org)
  • All reagents supplied need to be stored at 2 °C - 8 °C, unopened reagents will retain reactivity until expiration date. (elisakits.co.uk)
  • Using the established model, meningeal tissue constructs were exposed to physiological and pathophysiological flow conditions simulating the impaired CSF flow associated with optic nerve compartment syndrome and RNA sequencing was performed. (biomedcentral.com)
  • In addition, this technique measures the real-time physiological properties of a tissue, which is very useful. (jove.com)
  • To begin, place the desired segments of intestinal tissue collected from claudin-15 knockout mice into ice cold bubbled Ringer's solution and trim away the fat and connective tissue. (jove.com)
  • To strip the muscle layer, pour two to three milliliters of fresh ice cold bubbled Ringer's solution into a silicone rubber-covered dissection plate under a dissection microscope and use the pins to secure the ends of one intestinal tissue segment in the dish with the mucosal side facing down. (jove.com)
  • Piglets infected with PDCoV were most affected, whereas administration of EP reduced diarrhea incidence, alleviated intestinal lesion, and decreased viral load in feces and tissues. (biomedcentral.com)
  • Preincubation pertaining to 1 l from the intestines and stomach SM strip using 1 mg/ml filtered IgG from the sera of four years old main Social security sufferers (SS IgG) substantially inhibited carbachol-induced sleek muscle tissue contraction (CISC) on the range of CCh concentrations of mit, although IgG from healthful handles had tiny effect. (peptide-solubility.com)
  • CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. (thermofisher.com)
  • Bright Peak's Immunocytokines allow tissue- and cell-specific targeting of the cytokine payload with the added potential for synergistic efficacy through potency-enhancing avidity effects, the company said. (biospace.com)
  • Adenocarcinoma and control cases were assessed using the tissue microarray technique. (unesp.br)
  • Apoptosis is a double-edged sword mechanism, which is used by the host to eliminate viruses and is manipulated by the virus to cause cytopathic effects in vitro and/or tissue damage in vivo [ 10 ]. (biomedcentral.com)
  • According to the GLOBOCAN 2020 estimates of incidence and mortality for 36 cancers in 185 countries produced by the International Agency for Research on Cancer (IARC), an estimated 19.3 million new cancer cases and almost 10.0 million cancer deaths occurred worldwide in 2020, and GC ranked fifth (5.6%) and fourth (7.7%), respectively ( 2 ). (frontiersin.org)
  • My results suggest that, rather than forming a hybrid channel with novel characteristics, claudin-2 and claudin-10a form pores in parallel. (ku.edu)
  • Moreover, the continued advances in detectors and the emergence of digital cadmium-zinc-telluride cameras have allowed for improved extrinsic resolution ( 2 ), increased count sensitivity, and reduced scan times ( 3 ). (snmjournals.org)
  • Previous studies have reported that maternal SARS-CoV-2 infections and immunological responses elevate the risk of altered fetal brain development, fetal death, growth restrictions and severe pathologies such as intraventricular hemorrhages and pneumonia. (news-medical.net)
  • In addition, SARS-CoV-2 was present sparsely in the placental, umbilical cord and amniotic tissues, indicating SARS-CoV-2 presence in maternal and fetal tissues. (news-medical.net)
  • Synthekine's lead candidates are its selective IL-2 partial agonist, STK-012, as well as its orthogonal IL-2 and CD-19 CAR-T system, STK-009, and SYNCAR-001. (biospace.com)
  • After trimming away the fat and connective tissue, cut along the mesenteric attachments to open each segment longitudinally, then return the segments to the ice cold Ringer's solution and wash the pieces thoroughly. (jove.com)
  • Membrane enriched extracts (40 µg lysate) of Caco-2 (Lane 1), Caco-2 treated with IFN Gamma (50 ng/mL for 24hrs) (Lane 2), A-431 (Lane 3), HEL 92.1.7 (Lane 4) and Jurkat (Lane 5). (thermofisher.com)
  • The mean values of surface areas in the RBC clusters were 0.02 mm 2 and 0.05 mm 2 among non-hemorrhagic and hemorrhagic specimens, respectively, with the biggest cluster having a surface area of 1.3 mm 2 . (news-medical.net)