• But while some companies are developing chips to mimic diseased organs, most are still testing whether existing drugs behave in the chips as they do in healthy human tissues. (nature.com)
  • Regenerative medicine is a promising therapeutic strategy for the repair and replacement of diseased or injured tissues and organs. (ncsu.edu)
  • Tissue engineering (TE) is a promising strategy for replacing, repairing or regenerating damaged tissues and organs. (nature.com)
  • The intense, focused light of lasers with these colors can be used for a wide variety of unique therapeutic interventions: making incisions, cauterizing and sealing wounds, and selectively heating or even vaporizing specific regions of organs and tissues. (nationalacademies.org)
  • Unique to each connective protein array is the path of production, its relative proportion, and distribution in tissues or organs. (medscape.com)
  • Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. (wikipedia.org)
  • Since frozen tissue sections don't go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen tissues retain their native characteristics much more than in paraffin embedded tissues. (wikipedia.org)
  • Here, we used the Xenium Analyzer (10x Genomics) to characterize the gene expression profiles of formalin fixed & paraffin embedded (FFPE) cancer tissue sections with single cell and spatial resolution. (bmj.com)
  • In contrast, certain colors or wavelengths in the infrared (IR) and ultraviolet (UV) regions are absorbed strongly by biological tissues. (nationalacademies.org)
  • The electrodes rub against the surrounding brain tissue, which leads to scarring around the electrode known as a "kill-zone" of dead nerve cells - the outcome is that you have damaged what you want to study. (lu.se)
  • Lung Tumor Tissue Array - 64 Different Lung tumors. (conzort.com)
  • this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. (wikipedia.org)
  • Scientists who have been developing antibodies for therapeutic purpose all need their preliminary results from frozen tissues to get approval from FDA. (wikipedia.org)
  • But their weak adhesion to tissues is a major challenge for controlled drug delivery over the long run or for biosensing, which involves using a device to detect DNA, enzymes, antibodies and other health indicators. (sciencedaily.com)
  • The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. (sigmaaldrich.com)
  • Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
  • The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. (rndsystems.com)
  • Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. (rndsystems.com)
  • Connective Tissue Gene Tests (CTGT) has added 12 new assays to its menu of molecular diagnostics for inherited disorders affecting connective tissues. (genomeweb.com)
  • Buffered formalin solutions cross link proteins and nucleic acids when they are used for fix tissues. (wikipedia.org)
  • Therefore, many scientific experimental results from formalin fixed tissues are not reliable. (wikipedia.org)
  • Schoenberg Fejzo M, Slamon DJ: Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. (wikipedia.org)
  • The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues. (nih.gov)
  • Current and future work in this field is focused on identifying specific forces, stresses, and strains at the cellular and tissue level through both experimental and computational approaches, and analyzing the role of specific proteins through fluorescence-based investigations and knockdown models. (ncsu.edu)
  • Each tissue and organ system has an array of connective proteins. (medscape.com)
  • EDS is caused by various abnormalities in the synthesis and metabolism of collagen and other connective-tissue proteins in the ECM, such as elastin, proteoglycans, and macromolecular proteins. (medscape.com)
  • Physical therapy guided by a therapist who is experienced in working with patients with connective-tissue abnormalities and joint dysfunction can be very helpful in the management of long-term health. (medscape.com)
  • Individuals with Ehlers-Danlos syndrome (EDS) demonstrate connective-tissue abnormalities due to defects in the inherent strength, elasticity, integrity, and healing properties of the tissues. (medscape.com)
  • A lung-on-a-chip, for instance, might consist of a layer of cells exposed to a blood-like medium on one side and air on the other, hooked up to a machine that stretches and compresses the tissue to mimic breathing. (nature.com)
  • With 4D printing, engineers have created tiny needles that mimic parasites that attach to tissues and could replace hypodermic needles, according to a new study. (sciencedaily.com)
  • These materials have mechanical properties that mimic neural tissue more closely than traditional micro wires used in current cochlear and deep brain stimulating implants. (asme.org)
  • Cytomics Frozen section procedure MicroArray and Gene Expression (MAGE) Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. (wikipedia.org)
  • The simplicity of this design enabled the development of multiple microtissue arrays (5, 12, and 30 microtissues) by co-culturing endothelial cells, stromal cells, and fibrin within the microchambers over two and three week periods. (nih.gov)
  • The 477-gene panel helped resolve biologically distinct subgroups of immune, cancer, and tissue-specific cells. (bmj.com)
  • Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. (ncsu.edu)
  • How do cells within these mechanically active tissues interpret external loads imposed on their extracellular environment, and, how are cell–substrate interactions converted into biochemical signals? (ncsu.edu)
  • Resident memory (T RM ) cells are a distinct tissue-localized T cell lineage that is crucial for protective immunity in peripheral tissues. (frontiersin.org)
  • Recent studies in mouse tumor models have shown that T RM cells are induced by cancer vaccines delivered in peripheral tissue sites, or by the depletion of regulatory T cells. (frontiersin.org)
  • Such tumor-specific T RM cells are recognized as both necessary and sufficient for long-lived protection against tumors in peripheral tissue locations. (frontiersin.org)
  • Following effective priming in lymph nodes, T cells traffic to tumors and other peripheral tissues. (frontiersin.org)
  • whereas T EM cells were shown to recirculate predominantly through blood, and peripheral tissues ( 10 ). (frontiersin.org)
  • In the early 2000's, large and persistent populations of antigen (Ag)-specific CD8 T cells in peripheral tissues were initially classified as T EM cells in recirculation from the blood ( 8 , 11 ). (frontiersin.org)
  • The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. (prosci-inc.com)
  • To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
  • The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
  • Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
  • 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. (hindawi.com)
  • Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
  • TE strategies typically incorporate cells, biomaterials and signals (e.g. growth factors), with the goal of developing a construct that once implanted will promote tissue regeneration. (nature.com)
  • These dyes or biomarkers can be used in conjunction with visible and near-infrared light to highlight specific cell and tissue types, such as metastatic cancer cells circulating in the bloodstream. (nationalacademies.org)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • In cases for which PCV2 is considered causative, immunohistochemical and in situ hybridization (ISH) analyses demonstrate large amounts of PCV2 antigen or nucleic acids in the cytoplasm of macrophages and dendritic cells in the depleted follicles in lymphoid tissues ( 4 , 5 ). (cdc.gov)
  • Macrophages are critical effector cells in host defense and release a diverse array of cellular mediators including cytotoxic and proinflammatory cytokines that contribute to tissue injury. (cdc.gov)
  • The ECM is defined as the outer cell components of tissue that provide structural support to the cells. (medscape.com)
  • Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
  • This set-up is also equipped with a Multielectrode Array (MEA) system and there is a possibility to perform micromanipulator assisted collection of single cells for qPCR (Patch-Seq). (lu.se)
  • Patch clamp recording in vitro (cell cultures) and, to some extent, ex vivo (tissue preparations) to study intrinsic electrical properties of single cells, such as resting membrane potential, membrane resistance, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity, and to identify, characterize and quantify synaptic transmission. (lu.se)
  • Optogenetic manipulation of cell function to study, e.g. synaptic profile of isolated populations of the cells in cell cultures and tissue preparations. (lu.se)
  • Optical imaging of live cells in cultures and tissue preparations to monitor intracellular Ca 2+ fluctuations and to identify population of interest. (lu.se)
  • We measure cellular forces in cells and tissues with high spatial resolution using arrays of vertical nanowires. (lu.se)
  • This opens the way to high-resolution spatial analysis of cells and tissues. (lu.se)
  • Tests commonly employed in frozen tissue array include immunohistochemistry, and in situ hybridization. (wikipedia.org)
  • Studying tumor tissues with high resolution in situ technologies further unlocks the potential to profile the tumor microenvironment and cancer cell heterogeneity at a subcellular resolution. (bmj.com)
  • Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. (prosci-inc.com)
  • Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to allow simultaneous histological analysis. (wikipedia.org)
  • Consequently, frozen tissue array should be the best tool for high throughput analysis on this purpose. (wikipedia.org)
  • Each frozen tissue array block can be cut into 100-500 sections, which can be subjected to independent tests. (wikipedia.org)
  • Methods We used Xenium Analyzer to profile 5 μm FFPE tumor sections from various tissue types in a tissue array format. (bmj.com)
  • Results Cell type populations at subcellular resolution were identified and labeled in each FFPE tumor tissue section using unsupervised clustering. (bmj.com)
  • Conclusions The Xenium Human Multi-Tissue and Cancer Gene Expression Panel and custom add-on genes allowed us to profile multiple human tissue types with subcellular resolution in a range of FFPE tumor tissues on a single slide. (bmj.com)
  • Fiber-reinforced scaffolds for tissue engineering and regenerative medicine: use of traditional textile substrates to nanofibrous arrays. (ncsu.edu)
  • Scaffold based strategies for regenerative medicine have focused on the use of three dimensional, biocompatible, biodegradable structures to provide an adequate template for ex vivo cell expansion and maturation, native tissue ingrowth, and restoration of the original tissue qualities with respect to the tissue's biochemical constituents, morphology, form, and function. (ncsu.edu)
  • Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. (nih.gov)
  • Complex processes in biology involve a wide array of cell types, as do diseases such as cancer, so having the ability to accurately understand cell fates is critical to elucidate the underlying molecular mechanisms associated with normal and abnormal tissue growth. (genengnews.com)
  • Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. (lu.se)
  • Tissue microarrays from frozen tissues-OCT technique. (wikipedia.org)
  • The microneedle array has backward-facing barbs that interlock with tissue when inserted, enhancing adhesion. (techbriefs.com)
  • This paper reports a polydimethylsiloxane microfluidic model system that can develop an array of nearly identical human microtissues with interconnected vascular networks. (nih.gov)
  • Pantomics Array Description: IHC control tissue array for AFP, 4 cases, 8 samples (1.5mm) Species: Human Anatomic. (delos.info)
  • The slides were incubated overnight with the Xenium Human Multi-Tissue and Cancer Gene Expression Panel and a custom panel of 100 additional genes, for a total of 477 genes which broadly targeted cell types across multiple human tissue types. (bmj.com)
  • To work safely by adhering to all University policies and practices, including preparing and following laboratory risk assessments, and complying with Health and Safety policies, ethical approval processes and Human Tissue Act guidelines. (ucl.ac.uk)
  • The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. (sigmaaldrich.com)
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. (sigmaaldrich.com)
  • Protein array of 364 human recombinant protein fragments. (sigmaaldrich.com)
  • But before neural prosthetics can advance, engineers will be called on to make innovative use of materials to design and fabricate devices that allow sustained electronic functioning in the harsh environment of the human body, without causing tissue infection and other serious adverse conditions. (asme.org)
  • Research efforts have focused on enhancing the performance of various types of materials used in neural prosthetics, in addition to developing interface technologies that enable the micro devices to be safely implanted in human tissue for long periods. (asme.org)
  • We think our 4D-printed microneedle array will allow for more robust and sustained use of minimally invasive, pain-free and easy-to-use microneedles for delivering drugs, healing wounds, biosensing and other soft tissue applications," said senior author Howon Lee, an assistant professor in the Department of Mechanical and Aerospace Engineering in the School of Engineering at Rutgers University-New Brunswick. (sciencedaily.com)
  • We think our 4D-printed microneedle array will allow for more robust and sustained use of minimally invasive, pain-free, and easy-to-use microneedles for delivering drugs, healing wounds, biosensing, and other soft tissue applications," says researcher Howon Lee. (techbriefs.com)
  • Pantomics Array Description: 35 types of normal tissues based on the FDA recommendation for antibody cross-reactivity. (delos.info)
  • A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. (rndsystems.com)
  • Why Use an Antibody Array to Detect Multiple Cytokines? (rndsystems.com)
  • The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample. (rndsystems.com)
  • Frozen tissue cores with 2 mm diameter from the regions of interest are removed from frozen tissue OCT blocks at different freezing temperature since each tissue type has their own temperature preference at frozen stage. (wikipedia.org)
  • Then all the frozen tissue cores are inserted in a recipient OCT frozen block in a precisely spaced, array pattern. (wikipedia.org)
  • 2. Most TMA slides were not coated with an extra layer of paraffin (tissue cores can be easily seen on the glass). (tissuearray.com)
  • 10 μm single tissue slides are available upon request. (prosci-inc.com)
  • To prevent tissue detachment during antigen retrieval, unbaked slides must be baked for at least 30 to 120 minutes at 60°C . before putting into xylene for de-paraffinization. (tissuearray.com)
  • Extracellular field potential recordings, spontaneous or evoked, to study general network activity, or synaptic plasticity ex vivo in brain tissue preparations (predominantly in hippocampus - LTP, LTD). (lu.se)
  • The problem with most of the electrodes used today is that they are stiff and often quite rough, which means they irritate the brain tissue when the brain moves, which it does more or less the whole time, such as when we breathe or move our head. (lu.se)
  • It was shown that thin, flexible electrodes with approximately the same density as brain tissue caused the least damage. (lu.se)
  • Gene expression variations for organisms, tissue, and disease states have historically been quantified using an array of techniques. (genengnews.com)
  • The epigenome consists of chemical modifications attached to our DNA, which can be altered due to environmental factors, e.g., exercise, and thereby provides dynamic gene regulation and gives each cell or tissue its unique identity and functions. (lu.se)
  • We have generated DNA methylation and mRNA expression array data from adipose tissue and skeletal muscle before and after a 6-months exercise intervention. (lu.se)
  • The DNA, RNA, and protein within the tissues are damaged in various levels during the fixation. (wikipedia.org)
  • In this field, nanoparticles have notable importance due to their unique properties and characteristics, which are useful in different applications, including tissue engineering, biomarkers, and drug delivery systems. (mdpi.com)
  • [ 7 ] The specific characteristics of a particular form of EDS stem from the tissue-specific distribution of various components of the extracellular matrix (ECM). (medscape.com)
  • Multielectrode Array (MEA) recordings of extracellular field potentials from cell cultures and tissue preparations. (lu.se)
  • Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. (lu.se)
  • Background Tissue array technology enables screening of multiple tissue samples on a single microscopic slide and is an assistive tool in the field of oncology, given its ability to investigate cancer markers in a high-throughput manner. (bmj.com)
  • One of the potential limitations associated with the use of alginate for in vivo tissue regeneration is that it is in general non-degradable by mammals, as they lack the enzymes needed to break down the polymer chains 21 . (nature.com)
  • Matrix stiffness is a key determinant of mesenchymal stem cell (MSC) differentiation, suggesting that modulation of alginate bioink mechanical properties represents a promising strategy to spatially regulate MSC fate within bioprinted tissues. (nature.com)
  • We next explored if spatially modulating the stiffness of 3D bioprinted hydrogels could be used to direct MSC fate inside printed tissues. (nature.com)
  • We investigate epigenomic and transcriptomic changes in skeletal muscle and adipose tissue and their association with obesity, insulin resistance, and metabolic risk. (lu.se)
  • Breast cancer tissue array containing triple negative (TNBC) cases, including pathology grade, TNM, clinical stage and IHC results of ER, PR and Her-2. (tissuearray.com)
  • The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. (rndsystems.com)
  • The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (rndsystems.com)
  • Ehlers-Danlos syndrome (EDS) consists of a group of inherited heterogeneous disorders that share a common decrease in the tensile strength and integrity of the skin, joints, and other connective tissues. (medscape.com)
  • [ 1 ] This group of connective-tissue disorders is characterized by abnormal collagen synthesis causing hyperextensibility of the skin, hypermobility of the joints, and tissue fragility, as is seen by easy bruising and delayed wound healing with atrophic scarring. (medscape.com)
  • The main approach for this method is the fabrication and use of scaffold materials to act as a surrogate framework and promote cell-seeded populations to develop into a mature and functional tissue. (ncsu.edu)
  • Inspired by these examples, Rutgers engineers developed a microneedle that interlocks with tissue when inserted, enhancing adhesion. (sciencedaily.com)
  • Using chicken muscle tissue as a model, the researchers showed that tissue adhesion with their microneedle is 18 times stronger than with a barbless microneedle. (sciencedaily.com)
  • In conclusion, the inhibition of the inflammatory response resulted in delayed phagocytosis of the necrotic myofibers and delayed the repair process of the injured muscle tissue. (cdc.gov)
  • DNA lesions such as 8-nitroguanine and 8-oxodG with mutagenic properties occur in several types of inflammation-related cancer tissues [ 10 ]. (hindawi.com)
  • In nature, some insects and other organisms have developed microscopic features that adhere to tissue, such as the microhooks of parasites, barbed stingers of honeybees and scaled quills of porcupines. (sciencedaily.com)
  • For its array-based tests, the company relies on its High-Density Targeted Array platform, an Agilent Technologies-manufactured chip for copy number variation detection. (genomeweb.com)
  • The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. (rndsystems.com)
  • After detection, the array data were quantitated to generate a protein profile (histogram). (rndsystems.com)
  • The detection of a circovirus in tissues of dogs expands the known tropism of these viruses to a second mammalian host. (cdc.gov)
  • In experiments with auditory prosthetic applications, the microelectrodes comprising the neural interface are embedded in the polymer, which allows the device to move naturally and conform to live tissue in which it is implanted. (asme.org)