• Genetic and structural characterization of the human mitochondrial inner membrane translocase. (nih.gov)
  • Dysfunction of the mitochondrial electron transport chain (mETC) is a major cause of human mitochondrial diseases. (biorxiv.org)
  • Predicted to be part of TIM23 mitochondrial import inner membrane translocase complex. (nih.gov)
  • Predicted to be integral component of mitochondrial inner membrane. (nih.gov)
  • Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. (nih.gov)
  • V-ATPase is made of two subcomplexes: a cytosolic V 1 that carries out ATP hydrolysis, and a membrane bound V o that is responsible for proton translocation. (upstate.edu)
  • Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
  • The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. (nih.gov)
  • Wiedemann N, van der Laan M, Hutu DP, Rehling P, Pfanner N (2007) Sorting switch of mitochondrial presequence translocase involves coupling of motor module to respiratory chain. (uni-goettingen.de)
  • Predicted to be involved in protein import into mitochondrial matrix. (nih.gov)
  • Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. (nih.gov)
  • The mETC is comprised of a series of four large inner mitochondrial membrane (IMM) complexes (CI-CIV) that assemble into supercomplexes of defined stoichiometry ( Letts and Sazanov, 2017 ). (biorxiv.org)
  • We have analyzed the interaction of Hep1 with mitochondrial Hsp70 (Ssc1) and the determinants in Ssc1 which make it prone to aggregation. (cipsm.de)
  • To identify determinants of mETC function, we screened a genome-wide human CRISPRi library under oxidative metabolic conditions with selective inhibition of mitochondrial Complex III and identified OCIA domain-containing protein 1 (OCIAD1) as a Complex III assembly factor. (biorxiv.org)
  • For example, alkaline vacuole/lysosome are deficient in autophagy, Golgi pH regulates its ability to glycosylate proteins and failure to maintain endosomal pH perturbs with its ability to recycle receptors to the Plasma membrane or, the trans-Golgi. (upstate.edu)
  • Predicted to enable protein transmembrane transporter activity. (nih.gov)
  • Correspondingly, differentiation was associated with decreased expression of the mitochondrial citrate transporter (CIC). (biomed.news)
  • Journal cover: V-ATPase is regulated by disassembly into soluble V 1 (shown in cyan) and membrane integral V o (shown in gold) subcomplexes. (upstate.edu)
  • Taken together, these results highlight a central role for mitochondrial citrate metabolism in orchestrating histone acetylation and gene expression during trophoblast differentiation. (biomed.news)
  • The cryo-EM analysis also revealed a population of free V 1 bound to Oxidation Resistance 1 (Oxr1p), a poorly characterized protein that has been implicated in protecting cells from oxidative stress. (upstate.edu)
  • shown in red), a poorly characterized protein implicated in oxidative stress response. (upstate.edu)
  • Mitochondria are double membrane-bound organelles of endosymbiotic origin that produce most of the ATP in eukaryotic cells through oxidative phosphorylation (OXPHOS) ( Mitchell, 2011 ). (biorxiv.org)
  • These methods dramatically increase the likelihood of producing proteins that cannot fold or assemble appropriately. (cipsm.de)
  • Organelles are also identified by the presence of specific Phosphoinositide (PI) lipids on the cytosolic leaflet of their membrane. (upstate.edu)
  • CRISPR/Cas9 disruption of the mitochondrial citrate carrier showed that CIC is required for biochemical differentiation of trophoblasts. (biomed.news)
  • Unlike reversible disassembly, which requires ATP hydrolysis on V 1 to break protein-protein interactions, Oxr1p mediated disassembly of V-ATPase is ATP independent and therefore a novel mode of activity regulation. (upstate.edu)
  • Specific distribution of PI lipids is regulated, either by the localization of a kinase-phosphatase pair or, by vesicular and non-vesicular lipid transport. (upstate.edu)
  • Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)
  • Richter-Dennerlein R, Dennerlein S, Rehling P (2015) Integrating mitochondrial translation into the cellular context. (uni-goettingen.de)
  • Mitochondria play a critical role in differentiation events in cellular systems, thus we hypothesized that mitochondrial metabolism played a central role in trophoblast differentiation. (biomed.news)
  • The telomeres consist of repeating sequences and a set of special proteins, which interact with these repeats and spatially organize them in a specific manner, resulting in the formation of the nucleoprotein complex known as telomeric heterochromatin [ 6 , 7 ]. (actanaturae.ru)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Naumenko N, Morgenstern M, Rucktäschel R, Warscheid B, Rehling P (2017) INA complex liaises the F 1 F o -ATP synthase membrane motor modules. (uni-goettingen.de)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • We find that OCIAD1 is an inner mitochondrial membrane protein that forms a complex with supramolecular prohibitin assemblies. (biorxiv.org)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • As the assembly was carried out in the absence of ATP, this suggests that formation of the holoenzyme from the individual subcomplexes is solely driven by protein-protein interactions at the interface. (upstate.edu)