In enzymology, a thymidylate synthase (FAD) (EC 2.1.1.148) is an enzyme that catalyzes the chemical reaction 5,10-methylenetetrahydrofolate + dUMP + FADH2 ⇌ {\displaystyle \rightleftharpoons } dTMP + tetrahydrofolate + FAD The 3 substrates of this enzyme are 5,10-methylenetetrahydrofolate, dUMP, and FADH2, whereas its 3 products are dTMP, tetrahydrofolate, and FAD. (wikipedia.org)
We investigated the clinical significance of E2F1 gene expression in relation to its target genes, thymidylate synthase ( TS ) and Survivin , in case of non-small-cell lung cancer (NSCLC). (aacrjournals.org)
5-FdUMP inhibits thymidylate synthase (key enzyme in DNA synthesis), which leads to accumulation of dUMP, which then gets misincorporated into the DNA in the form of 5-FdUTP resulting in inhibition of DNA synthesis and function with cytotoxic DNA strandbreaks. (medscape.com)
Thymidylate synthase (TYMS) enhancer region genotype-directed phase II trial of oral capecitabine for 2nd line treatment of advanced pancreatic cancer. (cdc.gov)
Thymidylate synthase genotype-directed neoadjuvant chemoradiation for patients with rectal adenocarcinoma. (cdc.gov)
Thymidylate synthase gene copy number as a predictive marker for response to pemetrexed treatment of lung adenocarcinoma. (cdc.gov)
Enzyme3
Fluorouracil inhibits thymidylate synthetase, the key enzyme involved in the biosynthesis of pyrimidine. (lekarstwo.ru)
TFD mono-phosphate also prevents the thymidylate synthetase enzyme reversibly, which is useful in DNA replication 4-6 . (ijpsr.com)
The possible relevance of the sulphur geometry in two of the compounds to certain structural aspects of the action of the enzyme thymidylate synthetase is also pointed out. (iisc.ac.in)
Inhibits thymidylate synthetase1
Capecitabine is a prodrug that is converted to fluorouracil following oral administration, which in turn inhibits thymidylate synthetase, blocking the m. (arogga.com)
Methylation of deoxyuridylic acid2
Undergoes hydrolysis in liver and tissues to form the active moiety (fluorouracil), inhibiting thymidylate synthetase, which in turn blocks methylation of deoxyuridylic acid to thymidylic acid. (medscape.com)
Interferes with DNA synthesis by blocking methylation of deoxyuridylic acid, inhibiting thymidylate synthetase and subsequently cell proliferation. (medscape.com)
Deoxyuridylic acid to thymidylic acid1
It interferes with DNA synthesis by blocking the THYMIDYLATE SYNTHETASE conversion of deoxyuridylic acid to thymidylic acid. (ucdenver.edu)
TYMS1
Decreased expression of six RNA species was seen at various time points in all cell strains analyzed, these were: plasminogen activator (PLAT), centromere protein F (CPF), replication factor C (RFC3), thymidylate synthetase (TYMS), a putative mitotic checkpoint kinase (BUB1) and a gene of unknown function (GenBank accession number AI859865). (cdc.gov)
Fluorouracil1
Calcium folinate enhances the inhibition of thymidine synthetase by increasing the intracellular folate pool, thus stabilizing the fluorouracil-thymidine synthetase complex and increasing its activity. (lekarstwo.ru)
Synthesis1
Inhibition of thymidylate synthesis is probably the single most important consequence in the overall reaction scheme. (medquizzes.net)
Thymidine1
Weekly subcutaneous injections of 1,2-dimethethyhydrazine into 6-week-old rats markedly increased thymidylate synthetase (TS) in the poorly differentiated, and thymidine kinase in the well-differentiated adenocarcinomas of the colon. (cancerprev.org)
Biosynthesis1
2011) Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria. (guidetopharmacology.org)
Gene1
The influence of methylenetetrahydrofolate reductase and thymidylate synthetase gene polymorphisms on lung adenocarcinoma occurrence. (cdc.gov)
Expression1
Resistance to 5-FU is a major clinical problem as mediated predominantly by over expression of Thymidylate Synthetase. (dcu.ie)
Effects of 5-fluorouracil1
Binds to and stabilizes ternary complex of FdUTP (intracellular active metabolite of fluoropyrimidines) and thymidylate synthetase (TS), augmenting cytotoxic effects of 5-fluorouracil. (medscape.com)
Inhibition1
It irreversibly binds to dihydrofolate reductase, inhibiting the formation of reduced folates, and thymidylate synthetase, resulting in inhibition of purine and thymidylic acid synthesis. (medicscientist.com)
MTHFR1
Therefore, SAM deficiency speeds up the use of 5,10-MTHF for the MTHFR reaction but reduces its availability for the alternate thymidylate synthetase reaction, which normally regulates the nucleotide balance of deoxyuridine monophosphate (dUMP) and deoxythymidine monophosphate (dTMP). (campuscrosswalk.org)
Tissues1
Undergoes hydrolysis in liver and tissues to form the active moiety (fluorouracil), inhibiting thymidylate synthetase, which in turn blocks methylation of deoxyuridylic acid to thymidylic acid. (medscape.com)
Active1
A filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant. (colorado.edu)