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Genes, Transgenic, SuicideThymidine KinaseGanciclovirSuicideGenetic TherapySuicide, AttemptedThymidineCytosine DeaminaseSimplexvirusFlucytosineProdrugsGenetic VectorsNucleoside DeaminasesSuicide, AssistedBystander EffectAntiviral AgentsHerpesvirus 1, HumanAdenoviridaeAcyclovirPhosphatidylinositol 3-KinasesMAP Kinase Signaling SystemTumor Cells, CulturedProtein-Serine-Threonine KinasesProtein KinasesTransfectionGene Transfer TechniquesThymidine PhosphorylaseGliosarcomaArabinofuranosyluracilAntimetabolitesCell LinePromoter Regions, GeneticCalcium-Calmodulin-Dependent Protein KinasesProtein Kinase InhibitorsOncolytic VirusesOncolytic VirotherapyEncyclopedias as TopicNewcastle disease virusHistory, 20th CenturyVirus ReplicationVaccinia virus
GanciclovirHerpesRecombinantInhibitorAdenovirusGliomaTransductionMesenchymalViral vectorsPromoterVitroVectorAntitumorVivoProdrugProteinClinicalHumanTherapeutic geneTumor cellsDeletionCellularGenomeCancersToxicityReporter geneMarkerLaboratoryCombinationTherapy systemEarlyPotentiallySystemMethodVirusTransferCancer cellsApproach
Ganciclovir7
  • To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer. (wjgnet.com)
  • Given the lack of effective treatments, we constructed a bifidobacterial recombinant thymidine kinase (BF-rTK) -ganciclovir (GCV) targeting system (BKV) to meet this requirement and to explore antitumor mechanisms. (biomedcentral.com)
  • The Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-TK + GCV) system is currently one of the best-studied tumor suicide gene therapy systems [ 9 - 11 ]. (biomedcentral.com)
  • A colorectal cancer model was used to decipher the molecular mechanism of BF-rTK + GCV (bifidobacterial recombination thymidine kinase/ganciclovir) using a human apoptosis antibody array kit in a murine cancer model in vivo . (biomedcentral.com)
  • Although the combination of herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) with ganciclovir (GCV) has been shown as a promising suicide gene treatment strategy for glioma, the almost immunodepressive dose of GCV required for its adequate in vivo efficacy has hampered its further clinical application. (hindawi.com)
  • Herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) in combination with ganciclovir (GCV) has been shown as one of the most promising suicide gene systems for tumor treatment in laboratory studies and is currently being tested in several clinical trials. (hindawi.com)
  • The suicide gene therapy group (14 rats) was subjected to in situ gene transfer with a recombinant adenovirus vector carrying the HSV-TK gene driven by CAG promoter (Ad.CAGHSV-TK) in gastric tumor, followed by the antiviral drug ganciclovir (GCV). (fujita-hu.ac.jp)
Herpes5
  • The herpes simplex virus type 1 (HSV-1) mutant 1716 lacks both copies of the ICP34.5 gene, and as a result is no longer able to replicate in terminally differentiated and non-dividing cells but will infect and cause lysis very efficiently in cancer cells, and this has proved to be an effective tumour-targeting strategy. (wikipedia.org)
  • Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
  • Suicide gene therapy, such as with the herpes simplex virus type 1 thymidine kinase (HSV-TK) gene, has been shown to exert antitumor efficacy in various cancer models in vitro. (fujita-hu.ac.jp)
  • Herpes virus thymidine kinase (HSV-tk) is used in suicide gene therapy. (umich.edu)
  • TICKLE", more scientifically known as Trigger-Induced Cell-Killing Lethal-Enzyme, started off as a suicide-gene from the Herpes Simplex Virus (HSV), in this case the gene encoding for Thymidine Kinase (TK). (ranomics.com)
Recombinant2
  • We used a replication defective recombinant retrovirus vector GINaTK (bearing HSV-TK gene) to make packaging cell PA317 produce progeny virions. (wjgnet.com)
  • To investigate further the oncolytic capacity of the Raccoonpox virus, we have generated a thymidine kinase (TK)-deleted recombinant Raccoonpox virus expressing the suicide gene FCU1 . (oncotarget.com)
Inhibitor5
  • p16 is a cyclin-dependent kinase inhibitor that has shown prognostic utility in some human cancers. (umich.edu)
  • p21 is a cyclin-dependent kinase inhibitor. (umich.edu)
  • p27 is a cyclin-dependent kinase inhibitor with many putative functions in normal and neoplastic cells. (umich.edu)
  • Thus, iPSCs provide new opportunities for drug Protein Tyrosine Kinase inhibitor screening and for studying the molecular and cellular mechanisms of cardiac pathophysiology in humans. (pdgfreceptor.com)
  • KU-60019 is a kinase inhibitor. (alzet.com)
Adenovirus2
  • The results suggest that adenovirus-mediated IL-12 gene transfer combined with Ad.CD followed by 5-FC treatment may be useful for treating cancers. (biomedcentral.com)
  • The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
Glioma3
  • Accordingly, rat C6 glioma cells were first transfected with pCDNA-TK and pCDNA-sr39TK, respectively, and the gene transfection efficacy was verified by immunocytochemistry and western blot analysis. (hindawi.com)
  • However, the dramatic progresses in gene therapy in the past decades have drawn increasing interests in the application of suicide gene system as a new treatment strategy for glioma. (hindawi.com)
  • Using a U87 glioma model in SCID mice, we show that the therapeutic capacity of the new CRISPR/Cas9-engineered hAMSCs is equivalent to that of therapeutic hAMSCs generated by introduction of the same therapeutic gene by transduction with a lentiviral vector previously published by our group. (nih.gov)
Transduction2
  • Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
  • Although virus-based gene therapy is a promising strategy to combat advanced prostate cancer, its current effectiveness is limited partially due to inefficient cellular transduction in vivo . (hindawi.com)
Mesenchymal2
  • Mesenchymal stem cell (MSC)-based gene therapy is a promising strategy in oncology. (ac.ir)
  • In this way, suicide genes have also found a good platform for better performance and have shown a stronger anti-tumor mechanism by riding on mesenchymal cells. (ac.ir)
Viral vectors3
  • The use of non-viral procedures, together with CRISPR/Cas9 genome-editing technology, allows the insertion of single-copy therapeutic genes at pre-determined genomic sites, overcoming safety limitations resulting from random gene insertions of viral vectors with potential for genome damage. (nih.gov)
  • In this study, we demonstrate that combination of non-viral gene delivery and CRISPR/Cas9-mediated knockin via homology-directed repair can replace the use of viral vectors for the generation of genetically modified therapeutic cells. (nih.gov)
  • E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
Promoter4
  • Lyoma virus enhancer, HSV-tk enhancer and promoter, HSV thymidine kinase. (umich.edu)
  • By using an expression vector, a 680 bp PSA promoter isolated from a prostate cancer patient demonstrated the prostate-specific expression of the reporter gene [ 9 ]. (hindawi.com)
  • The rat PB gene promoter (454 bp) contains ARE [ 11 ] and directs a hormonal and developmental regulated expression, of a heterologous gene, specifically to the prostate in transgenic mice [ 12 ]. (hindawi.com)
  • Transgenic mice bearing PB promoter fused to simian virus 40 large tumor antigen gene all consistently develop prostate adenocarcinoma [ 13 ]. (hindawi.com)
Vitro2
  • Finally, a subcutaneously C6 xenograft tumor model was established in the nude mice to test the in vitro efficacy of TK/GCV gene therapy. (hindawi.com)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
Vector2
Antitumor2
  • We have evaluated the antitumor effect of combining IL-12 with CD gene transfer in mice bearing renal cell carcinoma (Renca) tumors. (biomedcentral.com)
  • Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma. (kanazawa-u.ac.jp)
Vivo4
  • In the present study, we used FDG microPET as an in vivo evaluation method to assess the early therapeutic efficacy of the suicide gene therapy. (hindawi.com)
  • Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
  • Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
  • However, the therapeutic outcome appears not to be high as expected, presumably because the in vivo gene delivery efficiency is low. (biomedcentral.com)
Prodrug2
  • Several PET molecular imaging agents could be used for monitoring tumor responses to prodrug activation in gene therapy. (hindawi.com)
  • The enzyme/prodrug strategy using cytosine deaminase (CD) and 5-fluorocytosine (5-FC) has been used for cancer gene therapy. (biomedcentral.com)
Protein2
  • Human CD20 has been proposed as non-immunogenic protein with a dual function as selection marker and suicide gene. (haematologica.org)
  • The group deployed a 2-stage bacteria screen for finding the perfect HSK-TK-CH1 suicide protein. (ranomics.com)
Clinical5
  • Co-transfer of a suicide gene would provide a desirable safety switch in clinical TCR gene therapy. (haematologica.org)
  • Gastrointestinal cancer is the most important clinical target of gene therapy. (fujita-hu.ac.jp)
  • RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
  • A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
  • Therapeutic gene transfer affords a clinically feasible and safe approach to cancer treatment but a more effective modality is needed to improve clinical outcomes. (biomedcentral.com)
Human6
  • Our data demonstrate that HSV-TK/GCV suicide gene therapy system is effective for treating experimental human pancreatic cancer, which is largely resistant to the common therapies, so the suicide gene therapy system may be a potential treatment approach for pancreatic cancer. (wjgnet.com)
  • Meanwhile, three human digestive system xenograft tumor models (colorectal cancer colo320, gastric cancer MKN-45 and liver cancer SSMC-7721) and a breast cancer (MDA-MB-231) model were employed to validate the universality of BF-rTK + GCV in solid tumor gene therapy. (biomedcentral.com)
  • We investigated the use of human CD20 as suicide gene in T-lymphocytes. (haematologica.org)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • 2007-2011: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Laboratory of Structure and Function of Human Genes. (biomed-mipt.ru)
  • The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
Therapeutic gene2
  • Cancer gene therapy approaches include the direct killing of tumor cells by injecting a therapeutic gene into the tumor cell or employing vaccine strategies to deliver an immunomodulatory gene that stimulates the immune system to recognize tumor antigens [ 1 ]. (biomedcentral.com)
  • Therapeutic gene transfer using viral or nonviral vectors has been shown to be clinically feasible and safe. (biomedcentral.com)
Tumor cells1
  • We then transferred the HSV-TK gene to target cells SW1990 using these progeny virions, and treated these gene-modified tumor cells with GCV to study the sensitivity of the cells to GCV and their bystander effects by routine MTT-method. (wjgnet.com)
Deletion1
  • Deletion or mutation of the p16 gene leads to tumor development or tumor progression. (umich.edu)
Cellular2
  • The label for this gene pretty much says it all - cellular suicide. (ranomics.com)
  • But of course, in order for a cellular suicide gene to have therapeutic effect, the gene needs to be ON in a cancer cell and OFF everywhere else. (ranomics.com)
Genome1
  • The underlying hypothesis has been that differences in gene sequence between individuals could result in alteration in genome function that would create phenotypic differences that manifest themselves in mental or physical disorders. (biomedcentral.com)
Cancers1
  • Gene therapy for treating cancers has been intensively investigated. (biomedcentral.com)
Toxicity1
  • Marker genes enable detection and selection of T cells, whereas suicide genes enable selective destruction of T cells in case of toxicity. (ashpublications.org)
Reporter gene2
  • The aim of this study was to investigate whether DAbR1 can be expressed on lymphocytes and used as a reporter gene as well as a suicide gene for therapy of immune-related adverse effects. (snmjournals.org)
  • The favorable biodistribution and high image contrast observed warrant further studies of this new reporter gene. (snmjournals.org)
Marker1
  • We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)
Laboratory1
  • The shop Luminescence of Solids of the Medical Laboratory Technician retains metropolis emission, covering ECG, pertaining Holter consultants, PRACTISING steps and kinase remarks in challenges. (holiday-reisezentrum.de)
Combination1
  • We demonstrated that combination of suicide therapy and cell therapy leading m,to successful tumor inhibition. (ac.ir)
Therapy system1
  • 18] Tang Q,Zhang D,Wan M,et al.Experi mental study of the RV-HSV-TK/GCV suicide gene therapy system in gastric cancer[J].Cancer Biother Radiopharm,2007,22(6):755-761. (bbmc.edu.cn)
Early1
Potentially1
  • Meanwhile, Inhibitors,research,lifescience,medical the exclusion of comorbid diagnosis or a past history of suicide may have potentially excluded a group of most severe depressive patients. (sirtuinsignaling.com)
System1
  • Survival analysis results of multiple cancer models confirmed that BF-rTK + GCV system has a wide field of application in solid tumor gene therapy. (biomedcentral.com)
Method2
Virus1
  • It is becoming clear that vectors based upon adeno-associated virus (AAV) are the vectors of choice for certain gene therapy applications such as muscle delivery. (justia.com)
Transfer6
  • We previously reported in situ gene transfer and gene therapy for gastric cancer induced by N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG) in dogs. (fujita-hu.ac.jp)
  • To observe the histopathological changes at various times after HSV-TK/GCV gene therapy, groups of animals were sacrificed at 3, 8, and 30 days after gene transfer. (fujita-hu.ac.jp)
  • The HSV-TK gene was detectable in peripheral blood by PCR until 30 days after gene transfer. (fujita-hu.ac.jp)
  • Combined transfer of therapeutic genes with different modes of actions may be a means to this end. (biomedcentral.com)
  • The combined gene transfer increased splenic NK cell activity and IFN-γ production by splenocytes. (biomedcentral.com)
  • Combined transfer of genes with different mechanisms of action may potentate the therapeutic outcome. (biomedcentral.com)
Cancer cells1
Approach1
  • Directly targeting therapeutic suicide gene to a solid tumor is a hopeful approach for cancer gene therapy. (biomedcentral.com)