Thus oocyte. Medical search. Frequent questions

Many studies show that cumulus expansion is critical for the maturation of the oocyte because the cumulus complex is the oocyte's direct communication with the developing follicle environment. (wikipedia.org)
After 8 days of stimulation, 10000 IU of human chorionic gonadotropin (Pregnyl, NV Organon, Oss, Netherlands) was administered for oocyte maturation. (hindawi.com)
since all oocytes produced during this time are of equivalent "depth", the production-line hypothesis of postnatal oocyte maturation cannot logically explain increasing oocyte depth as females age. (science20.com)
We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. (springer.com)
Galvão A, Segers I, Smitz J, Tournaye H, De Vos M. In vitro maturation (IVM) of oocytes in patients with resistant ovary syndrome and in patients with repeated deficient oocyte maturation. (springer.com)
In experiment III, worms collected in spring were exposed to different temperature regimes to examine the effect of raising temperature on the synchronization of oocyte growth and maturation. (frontiersin.org)
The results showed worms collected in spring showed increased temperatures will increase oocyte maturation synchronicity. (frontiersin.org)
T mice, this can be explained by the preferential propagation of mtDNA during oocyte maturation, counterbalanced by purifying selection against high heteroplasmy levels. (cam.ac.uk)
van den Hurk R, Zhao J. Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. (harvard.edu)
Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. (elifesciences.org)
In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)
CiVP ultimately activated a maturation-promoting factor, leading to oocyte maturation via germinal vesicle breakdown. (elifesciences.org)
This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. (elifesciences.org)
Therefore, the regulatory mechanisms underlying oocyte maturation and ovulation control not only the reproduction of the respective organisms but also evolutionary processes across the animal kingdom. (elifesciences.org)
Some neuropeptides have been shown to induce oocyte maturation and ovulation (or spawning) in several species of invertebrates. (elifesciences.org)
These findings demonstrate that various neuropeptides are responsible for triggering oocyte maturation and ovulation in invertebrates, and suggest that oocyte maturation and ovulation and their underlying molecular mechanisms are regulated in both a species-specific and evolutionarily conserved fashion. (elifesciences.org)
After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
Roy SR, Wang J, Rana MR, Nakashima M, Tokumoto T. Characterization of membrane progestin receptor α (mPRα) of the medaka and role in the induction of oocyte maturation. (gene-tools.com)
We previously found that mutations in TUBB8 caused oocyte maturation arrest. (bmj.com)
Methods and results Patients with oocyte maturation arrest were sequenced with respect to TUBB8 . (bmj.com)
The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
However, the observed maximum growth rate, even when adjusted for the positive influence of temperature, allowed little scope for previtellogenic oocytes to complete maturation during a spawning period of 60 days. (alr-journal.org)
Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation. (bvsalud.org)
Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single- cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. (bvsalud.org)
T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. (bvsalud.org)

The oocyte, a large and complex cell, must be supplied with numerous molecules that will direct the growth of the embryo and control cellular activities. (wikipedia.org)
Such a method of live-cell lipid quantification has (i) experimental power in basic cell biology, (ii) practical utility for identifying developmental predictive biomarkers while advancing biology-based oocyte/embryo selection, and (iii) ability to yield rationally supporting technology for decision-making in rodents, domestic species, and human assisted reproduction and/or fertility preservation. (rsc.org)
Enhanced water recipe and enrichment for oocyte quality and embryo development in the African clawed frog (Xenopus laevis). (awionline.org)
Background TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. (bmj.com)
There is insufficient evidence to recommend a particular stimulation protocol for oocyte donors or certain donor characteristics in terms of embryo quality or success rates. (guidelinecentral.com)
The oocyte from a donor can be either recovered as an embryo after it has undergone fertilization within the body (by uterine lavage (washing) in a process called ovum transfer) or recovered as an egg and inseminated in vitro (in the laboratory) to yield an in vitro fertilized embryo. (ferty9.com)
11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
When a normal sperm cell fertilizes one of these oocytes, the resulting embryo has only one set of chromosomes. (medlineplus.gov)
The health of the mature oocyte and subsequent embryo is highly dependent on the oocyte mitochondria. (cdc.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)

The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. (springer.com)
However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. (life-science-alliance.org)
We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. (life-science-alliance.org)
Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
Experimental spindle transfer in metaphase II oocytes, also called mitochondrial replacement therapy, is a novel technology for preventing mtDNA transmission from oocytes to pre-implantation embryos. (infertile.com)
The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)
At laparotomy, ovulated oocytes and early-stage embryos were recovered from oviducts, and ovaries were removed for aspiration of oocytes and granulosa cells from unovulated follicles. (duke.edu)
Oocytes and embryos were stained for assessment of stage of development. (duke.edu)
Treatment with vitamin A altered development of oocytes and embryos by decreasing the percentage at the germinal vesicle stage and increasing the percentage at advanced stages. (duke.edu)

British Library EThOS: The use of Xenopus laevis oocytes as a transcription assay. (bl.uk)
Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and retina. (aspetjournals.org)
Electrical recordings were made in Xenopus oocytes to study the modulatory effects of steroids on gamma-aminobutyric acid (GABA) receptors expressed by RNA from mammalian brain and retina. (aspetjournals.org)
Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)

The oocyte is arrested in Meiosis II at the stage of metaphase II and is considered a secondary oocyte. (wikipedia.org)
These disturbances may lead ultimately to errors in chromosome segregation during the metaphase of meiosis, and that faulty segregation to oocyte aneuploidy. (cea.fr)
Mature oocytes become fertilizable, and their meiosis is arrested again at a species-specific stage: metaphase of the first division (MetI, many invertebrates), metaphase of the second division (MetII, most vertebrates), or G1-phase (some echinoderms and coelenterates) until fertilization. (elifesciences.org)
Spindle transfer from the patient's metaphase II oocyte into the cytoplasm of a donor oocyte. (infertile.com)

We report for the first time a case of ureteral injury that presented with delayed massive hematuria without urinary tract signs of peritoneal irritation 4 and 6 days after ultrasound-guided transvaginal oocyte retrieval. (hindawi.com)
Without gonadotropin priming and HCG trigger, ultrasound-guided transvaginal oocyte retrieval was performed. (springer.com)

Conclusions Our data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8 , underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF). (bmj.com)
Evidence-based outcomes after oocyte cryopreservation for donor oocyte in vitro fertilization and planned oocyte cryopreservation: a guideline. (guidelinecentral.com)

This study taking to account the limitations of studies in this area, aimed to compare the survival rate, fertilization capacity and developmental capacity of oocyte that cryo-preserved in open pulled and conventional straw. (scialert.net)
Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)

The oocyte donor may be anonymous and obtained through a donor egg agency, or, if desired, the donor can be designated by the patient (a friend or a sibling or other female relative with similar genetic makeup). (bcm.edu)
The oocytes (eggs) are removed from the donor and fertilized with sperm in the laboratory, then transferred to the recipient. (bcm.edu)
The procedure can be undertaken at virtually any time because the rate limiting issue is the age of the oocyte donor. (bcm.edu)
Oocytes may need to be cryo-stored in the event of unforeseen non-production of sperm during IVF therapy, allowing a more measured consideration of donor sperm use or other means of sperm retrieval. (scialert.net)
Heritable genetic diseases can be prevented with the use of donor oocytes. (bmj.com)
We report our experience in using donor oocytes from anonymous, matched, fertile donors in four women with heritable genetic disorders. (bmj.com)
Our results show that use of donor oocytes is a practical, successful, and currently available technique for the prevention of genetic disorders. (bmj.com)
G), with a long history of multiple undiagnosed pregnancy losses and deaths of offspring as a result of this disease, who underwent IVF after reconstitution of her oocytes by spindle transfer into the cytoplasm of enucleated donor oocytes. (infertile.com)
D) transfer of the removed spindle into the perivitelline space of the enucleated donor oocyte. (infertile.com)
It is recommended to tell recipients that previously cryopreserved donor oocytes are a reasonable option compared with fresh donor oocytes, given that there is good evidence that there are no significant differences in per transfer pregnancy rates compared with those with fresh donor oocytes. (guidelinecentral.com)
Recipients can be counseled that as the number of donor oocytes warmed increases, there is an associated increase in cumulative live birth rate. (guidelinecentral.com)
Recipients can be counseled that, based on limited evidence, neonatal outcomes appear similar between vitrified and fresh donor oocytes. (guidelinecentral.com)
There are no significant differences in per transfer pregnancy rates with cryopreserved vs. fresh donor oocytes. (guidelinecentral.com)
Title : Trends and Outcomes for Donor Oocyte Cycles in the United States, 2000-2010 Personal Author(s) : Kawwass, Jennifer F.;Monsour, Michael;Crawford, Sara;Kissin, Dmitry M.;Session, Donna R.;Kulkarni, Aniket D.;Jamieson, Denise J. (cdc.gov)
Oocyte Donor Program:The use of the female gamete, the egg or oocyte, for the treatment of certain disorders leading to female reproductive failure has only been possible since 1984. (ferty9.com)

An oocyte is produced in a female fetus in the ovary during female gametogenesis. (wikipedia.org)
CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. (elifesciences.org)
An oocyte is part the ovary development. (wikidoc.org)
As the oocyte matures in the postnatal mammalian ovary during folliculogenesis it increases exponentially in volume, and the oocyte mitochondria population proliferates to about 100 000 mitochondria per healthy, mature murine oocyte. (cdc.gov)
After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)

On Thursday, Oct. 12, Associate Professor in the Department of Molecular Biosciences at Northwestern University Sadie Wignall shared her findings on the mechanisms oocytes employ to regulate spindles without centrosomes during meiosis. (jhunewsletter.com)
The seminar , titled "Stabilizing a Dynamic Structure: Mechanisms that Maintain Acentrosomal Spindle Integrity During Oocyte Meiosis," was hosted by the Department of Biology at Hopkins for their seminar series. (jhunewsletter.com)
During her postdoctoral fellowship, Wignall began studying meiosis - a type of cell division that produces reproductive cells with half the number of chromosomes - occurring in female reproductive cells or oocytes. (jhunewsletter.com)
Meiosis in animal oocytes is arrested at prophase of the first division (ProI). (elifesciences.org)
nondisjunction -- a failure of proper homolog separation in meiosis I, or sister chromatid separation in meiosis II can lead to aneuploidy , in which the oocyte has the wrong number of chromosomes, for example 22,X or 24,X. This is the cause of conditions like Down syndrome and Edwards syndrome . (wikidoc.org)

The oocyte receives mitochondria from maternal cells, which will go on to control embryonic metabolism and apoptotic events. (wikipedia.org)
The partitioning of mitochondria is carried out by a system of microtubules that will localize mitochondria throughout the oocyte. (wikipedia.org)
In certain organisms, such as mammals, paternal mitochondria brought to the oocyte by the spermatozoon are degraded through the attachment of ubiquitinated proteins. (wikipedia.org)
Then, having established the full complement of mitochondria in the fully grown oocyte, there is no further increase of the mitochondrial population during early development. (nih.gov)
Toxicant effects on mammalian oocyte mitochondria. (cdc.gov)
Oocyte mitochondria are unique organelles that establish a founder population in primordial germ cells (PGCs). (cdc.gov)
Here we review the effects that toxic insults including chemotherapeutics, toxic metals, plasticizers, pesticides , polycyclic aromatic hydrocarbons (PAHs), and ionizing radiation can have on oocyte mitochondria. (cdc.gov)
This is very clearly a burgeoning field, as our understanding of oocyte mitochondria and metabolism is still relatively new, and we contend much more research is needed to understand the detrimental impacts of exposure to toxicants on oocyte mitochondria. (cdc.gov)

Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)

Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
After oocyte collection, immature oocytes were cultured in the IVM medium. (springer.com)

Oogenesis results in the formation of both primary oocytes during fetal period, and of secondary oocytes after it as part of ovulation. (wikipedia.org)
Scholars@Duke publication: Influence of vitamin A injection before mating on oocyte development, follicular hormones, and ovulation in gilts fed high-energy diets. (duke.edu)
For the current study, the hypothesis was that administration of vitamin A before ovulation would alter development of follicles and oocytes in a way favorable to enhanced embryonal survival. (duke.edu)

It was the first to be shown to alter oogenesis and lead to the formation of oocytes with an abnormal number of chromosomes (aneuploidy), in turn responsible for numerous miscarriages and fertility disorders. (cea.fr)

During oogenesis, the oogonia become primary oocytes. (wikipedia.org)
The formation of an oocyte is called oocytogenesis, which is a part of oogenesis. (wikipedia.org)
During the primary oocyte stage of oogenesis, the nucleus is called a germinal vesicle. (wikipedia.org)
Postnatal oogenesis through ongoing oogonial stem cell (OSC) mitosis explains increasing oocyte depth with age. (science20.com)
Three different experiments were conducted to examine the oocyte development of the polychaete Hediste diversicolor in response to changing photoperiod and temperature at three different periods of oogenesis. (frontiersin.org)

The only normal human type of secondary oocyte has the 23rd (sex) chromosome as 23,X (female-determining), whereas sperm can have 23,X (female-determining) or 23,Y (male-determining). (wikipedia.org)
Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). (springer.com)
Then sperm were added to living control, cryo-preserved in conventional and open pulled straws oocytes. (scialert.net)
Mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction. (oregonstate.edu)
Morphometric and morphokinetic differences in the sperm- and oocyte-or" by Lee-Sarose Orevich, Kate Watson et al. (edu.au)
All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by one embryologist (L-SO). (edu.au)
Timing parameters included 2nd polar body extrusion (tPB2), sperm-originated PN (tSPNa) or oocyte-originated PN (tOPNa) appearance, and PN fading (tPNF). (edu.au)
The only normal type of secondary oocyte has sex chromosomes 23,X (where sperm can be 23,X or 23,Y). (wikidoc.org)
A small number of cases of recurrent hydatidiform mole have been found to be caused by mutations in genes that play important roles in the production of oocytes and sperm cells. (medlineplus.gov)
during oocyte and sperm cell production. (medlineplus.gov)
The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)
At this point, intracytoplasmic sperm injection-injection of a single sperm into each oocyte-may be done, particularly if spermatogenesis is abnormal in the male partner. (msdmanuals.com)
After sperm are added, the oocytes are cultured for about 2 to 5 days. (msdmanuals.com)

Oocytes are rich in cytoplasm, which contains yolk granules to nourish the cell early in development. (wikipedia.org)
These mRNAs can be stored in mRNP (message ribonucleoprotein) complexes and be translated at specific time points, they can be localized within a specific region of the cytoplasm, or they can be homogeneously dispersed within the cytoplasm of the entire oocyte. (wikipedia.org)

Oocyte cryopreservation (egg-freezing), Kallen explained, is when a woman's eggs are removed from the ovaries and stored for future use as "a way of preserving reproductive ability in women (or individuals with ovaries)" and an alternative for "anyone who isn't planning pregnancy immediately" but might want to explore their options in the future. (uvm.edu)
In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)
We were sending young women to him for oocyte or ovarian cryopreservation back in the early 90s. (medscape.com)

We report a case of ureteral injury with delayed hematuria after transvaginal oocyte retrieval. (hindawi.com)
Transvaginal ultrasound-guided oocyte retrieval is a standard method in women undergoing IVF procedures. (hindawi.com)
Transvaginal ultrasound-guided oocyte retrieval was performed 36 hours later. (hindawi.com)
Objective: To assess the impact of class III obesity on outcomes and complications of transvaginal ultrasound-guided oocyte pickup (OPU). (bepress.com)

Thus, oocytes of many organisms are protected from oxidative DNA damage while storing up a large mass of substances to nurture the zygote in its initial embryonic growth. (wikipedia.org)
A recent genetic study tracing the origins of oocytes from the embryonic period throughout adulthood and adds new information to a growing controversy. (science20.com)
The proteins produced from the NLRP7 and KHDC3L genes are critical for normal egg cell (oocyte) development, which impacts embryonic development. (medlineplus.gov)

The cumulus-oocyte complex contains layers of tightly packed cumulus cells surrounding the oocyte in the Graafian follicle. (wikipedia.org)
We applied this approach to sequence the transcriptome of 17 prepubertal porcine oocytes and their corresponding cumulus cells. (auburn.edu)
We hypothesized that the transcript profiles of the cumulus cells and oocyte display distinct gene regulatory networks within the oocyte and cumulus cells. (auburn.edu)
We quantified 7277 genes expressed in the cumulus-oocyte complex. (auburn.edu)

With oocyte donation, any woman with a healthy uterus, regardless of the condition of her own eggs, can potentially bear a child with her partner. (bcm.edu)
Oocyte donation is also an option for those who are carriers of genetic diseases and wish to prevent the risk of either conceiving a child affected with the disease or who might be a carrier of a genetic disease. (bcm.edu)
Oocyte donation is proven, well-established technology with which there is considerable expertise. (bcm.edu)
Recipients can be counseled that live birth rates may be greater from vitrified oocytes that derive from donors who had prior successful outcome after a fresh cycle of oocyte donation. (guidelinecentral.com)
Egg donation is the process by which a woman provides one or several (usually 10-15) eggs (ova, oocytes) for purposes of assisted reproduction or biomedical research. (ferty9.com)

In a new assessment of the work by Shapiro and colleagues, reproductive biologists Dori Woods, Evelyn Telfer and Jonathan Tilly conclude that the most plausible explanation for these findings is that progenitor germ cells in ovaries continue to divide throughout reproductive life, resulting in production of new oocytes with greater depth as animals age. (science20.com)
Although these investigations were performed in mice, there is emerging evidence that oogonial stem cells are also present in the ovaries of reproductive-age women, and these cells possess the capacity, like their mouse counterparts, to generate new oocytes under certain experimental conditions. (science20.com)
A female reproductive cell, also called an oocyte or ovum. (cdc.gov)

In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. (lu.se)
A pregnancy that results from an abnormal oocyte cannot develop properly, resulting in recurrent hydatidiform mole. (medlineplus.gov)
In affected women, this can lead to the production of abnormal oocytes that do not contain chromosomes. (medlineplus.gov)

This stage the oogonia is now an Oocyte (pronounced oh'a (like Noah)-site ). (wikidoc.org)

The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. (jove.com)
Juvenile oocyte vitrification was a lot shorter in the generation interval in breeding programs. (jove.com)
The selection of the oocytes to submit at the vitrification procedures represent the first important step to warranty the successful application of this procedure. (jove.com)
The loading of the oocyte in the vitrification device used is an important and critical step. (jove.com)
In this method, the oocytes in the vitrification solution, less than 0 point milliliter are rapidly loaded with a glass capillary on top of the film strip. (jove.com)
1. Is Oocyte Freezing better than Oocyte Vitrification? (apollofertility.com)
But as the Oocyte Vitritifcation process is rapid and new, doctors now prefer Oocyte Vitrification. (apollofertility.com)

Below are some examples of maternally inherited mRNAs and proteins found in the oocytes of the African clawed frog. (wikipedia.org)
The oocyte is loaded with maternal mRNAs and proteins that control the cell maintenance and fate and the formation of the body plan prior to the onset of zygotic genome expression [ 3 , 4 ]. (biomedcentral.com)

Female infants have 6 to 7 million oocytes at 20 weeks of gestation, when progressive atresia occurs, resulting in 1 to 2 million oocytes at birth, approximately 25,000 at the age of 37 years, and 1000 at the age of 51 years, the average age of natural menopause in the United States. (ldysinger.com)

Among follicles matched by meiotic stage of oocyte, follicular fluid concentrations of progesterone, IGF-I, and PGF2alpha were greater in vitamin A-treated gilts than in controls, but treatment with vitamin A in vivo did not affect LH-stimulated or unstimulated secretion of progesterone by granulosa cells in vitro. (duke.edu)
Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)

Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). (harvard.edu)
About 34 hours after hCG is given, oocytes are retrieved by direct needle puncture of the follicle, usually transvaginally with ultrasound guidance or less commonly laparoscopically. (msdmanuals.com)
The oocytes compete with one another to become the dominant follicle and eventually ovulate to be released as an egg capable of being fertilized. (medscape.com)

An oocyte (UK: /ˈoʊəsaɪt/, US: /ˈoʊoʊ-/), oöcyte, or ovocyte is a female gametocyte or germ cell involved in reproduction. (wikipedia.org)

DNA damage occurring in oocytes, if not repaired, can be lethal and result in reduced fecundity and loss of potential progeny. (wikipedia.org)
Several aspects of vitellogenic oocyte growth and recruitement were examined as criteria to ascertain whether the annual fecundity can be determined in Solea solea just prior to spawning. (alr-journal.org)
Oocyte size frequency distribution from mid spawning and late spawning-spent sole from division IVc showed a hiatus in 9 out of 10 fish which became wider as the residual annual potential fecundity declined. (alr-journal.org)

Woods and colleagues say that "the recent work of Shapiro and colleagues is one of the first reports to offer experimental data consistent with a role for postnatal oocyte renewal in contributing to the reserve of ovarian follicles available for use in adult females as they age. (science20.com)

As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)

b) If continued proliferation of OSCs (red) and their subsequent differentiation into oocytes (blue) during postnatal life is superimposed on the production-line hypothesis, the emerging picture is consistent with a progressive increase in oocyte depth in females as they age. (science20.com)
Via genetic and chemical models, the team was able to propose that the deleterious effects of bisphenols on mature oocytes results from their ability to induce oxidative lesions on DNA at a key step in germinal cell differentiation during the fetal period. (cea.fr)

The number and quality of a woman's oocytes declines with age. (medscape.com)

The oocyte acquires developmental competence as it progresses through folliculogenesis. (auburn.edu)

These mutations cause a range of folding defects in vitro, different degrees of microtubule disruption upon expression in cultured cells and interfere to varying extents in the proper assembly of the meiotic spindle in mouse oocytes. (bmj.com)

An oocyte is a form of genetic material that can be collected for cryoconservation. (wikipedia.org)
The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
Use of oocytes from anonymous, matched, fertile donors for prevention of heritable genetic diseases. (bmj.com)
However, it is also possible that such genetic defects result in a smaller complement of oocytes during gonadal development. (ldysinger.com)

It was proposed that, in order to avoid damage to the DNA genome of the oocytes, the metabolism contributing to the synthesis of much of the oocyte's constituents was shifted to other maternal cells that then transferred these constituents to oocytes. (wikipedia.org)
Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)

In its study performed in a mouse model and published in Environmental Pollution , the team showed that fetal exposure to environmental concentrations of BADGE or BPAF caused oocyte defects in mature mice comparable to those provoked by fetal BPA exposure . (cea.fr)

The progressive loss of oocytes from fetal life through menopause is a normal process. (ldysinger.com)

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. (lu.se)
Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). (lu.se)

The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. (biologists.com)

A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. (life-science-alliance.org)
Independent clustering of co-expressed genes revealed critical biological functions for the oocyte, such as regulation of transcription, stem cell population maintenance, and insulin receptor signaling pathway. (auburn.edu)
The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation , such as mammalian oocytes . (bvsalud.org)

Some mutations in oocytes lead to infertility in the mature organism, whereas others, over a long period of time, may eventually lead to the emergence of novel species or subspecies. (elifesciences.org)
We investigated the effects of identified mutations in vitro, in cultured cells and in mouse oocytes. (bmj.com)
For example, oocytes harbouring any of three missense mutations (I210V, T238M and N348S) could extrude the first polar body. (bmj.com)
Surprisingly, oocytes from patients harbouring homozygous TUBB8 mutations that in either case preclude the expression of a functional TUBB8 polypeptide nonetheless contained identifiable spindles. (bmj.com)

While traditional thinking has held that female mammals are born with all of the eggs they will ever have, newer research has demonstrated that adult mouse and human ovaries contain a rare population of progenitor germ cells called oogonial stem cells capable of dividing and generating new oocytes. (science20.com)
Researchers traced the number of divisions a cell has undergone with age (its 'depth') and counted the number of times progenitor germ cells divided before becoming oocytes. (science20.com)
In a work published in Environmental Pollution , researchers from iRCM's LDG laboratory explored the effects of exposure to two bisphenol A substitutes, BADGE and BPAF, on murine germ cells, and more specifically on oocytes and their precursors. (cea.fr)

As the oocyte is a product of female gametogenesis, the maternal contribution to the oocyte and consequently the newly fertilized egg, is enormous. (wikipedia.org)
The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. (biologists.com)

Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. (tubitak.gov.tr)
It has been hypothesized that in gonadal dysgenesis various deletions on the X chromosome cause such rapid atresia of oocytes 5 that few girls with this condition reach puberty with functioning ovaries. (ldysinger.com)

Infertile women should be counseled that, based on a small number of births, neonatal outcomes appear similar after using their own previously cryopreserved oocytes compared with outcomes after the use of fresh oocytes. (guidelinecentral.com)
Neonatal outcomes appear similar with cryopreserved oocytes. (guidelinecentral.com)

During the growth of the oocyte, a variety of maternally transcribed messenger RNAs, or mRNAs, are supplied by maternal cells. (wikipedia.org)

11 h light) coupled with an endogenous timer synchronizes spawning of the first cohort of oocytes in Harmothoe imbricate L. The authors also mentioned that in H. imbricate vitellogenesis was favored by low temperature and increasing photoperiod. (frontiersin.org)
The growth rate of the leading oocyte cohort was 2.73 × 10 −5 mm 3 per day on 20 September and increased to 3.91 × 10 −3 at a mean diameter of 839 µ m by 10 February just prior to spawning. (alr-journal.org)

In normal women, at approximately 37.5 years of age, an accelerated atresia of the oocytes begins 1 , 2 ( Figure 1 ). (ldysinger.com)

The sequencing of single oocytes and their surrounding somatic cells revealed coordinated expression of hundreds of genes that formed functional regulatory networks. (auburn.edu)

Mitochondrial DNA heteroplasmy is modulated during oocyte development propagating mutation transmission. (cam.ac.uk)
A male euploid blastocyst was obtained from the reconstituted oocytes, which had only a 5.7% mtDNA mutation load. (infertile.com)

Oocyte Freezing is a way to protect your eggs and use them later in your life for pregnancy. (apollofertility.com)
So preserving the eggs with Oocyte Freezing makes perfect sense. (apollofertility.com)
4. What is the best age to secure the eggs with Oocyte Freezing? (apollofertility.com)
No. Oocyte Freezing just preserves your eggs. (apollofertility.com)

Within oocytes, the exact role of NLRP7 and KHDC3L proteins are not known. (medlineplus.gov)

Anatomy37

Organisms18

Diseases8

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology1

Phenomena and Processes64

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science4

Health Care2

Maturation29

Embryo11

Embryos10

Xenopus4

Metaphase4

Ultrasound-guided transvaginal oocyte re2

Vitro fertilization2

Fertilization2

Donor15

Ovary5

Meiosis5

Mitochondria8

Decline in the Number of Oocytes2

Immature oocytes2

Ovulation3

Formation of oocytes1

Oogenesis5

Sperm13

Cytoplasm2

Cryopreservation3

Transvaginal4

Embryonic3

Cumulus4

Donation5

Reproductive3

Abnormal3

Oogonia1

Vitrification7

MRNAs and proteins2

Weeks of gestat1

Follicular2

Follicle3

Ovocyte1

Fecundity3

Ovarian follicles1

Aneuploidy1

Differentiation2

Woman's1

Competence1

Meiotic spindle1

Genetic4

Metabolism2

Defects1

Fetal1

Drosophila2

Nucleus1

Regulation3

Mutations4

Germ cells3

Newly2

Ovaries2

Outcomes2

Maternal1

Cohort2

Atresia1

Genes1

Mutation2

Eggs4

Proteins1