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ThreonineThreonine DehydratasePhosphorylationProtein-Serine-Threonine KinasesPhosphothreonineAmino Acid SequenceMolecular Sequence DataSerinePhosphoprotein PhosphatasesIsoleucineHomoserine DehydrogenaseProtein KinasesAmino AcidsOkadaic AcidPhosphoserineAspartate KinaseProtein Phosphatase 2MutationBase SequenceEnzyme ActivationSignal TransductionSequence Homology, Amino AcidMutagenesis, Site-DirectedCell LineSubstrate SpecificityProtein Phosphatase 1PhosphopeptidesProto-Oncogene Proteins c-pim-1Cloning, MolecularBinding SitesL-Serine DehydrataseKineticsProtein BindingOxazolesPeptide MappingRecombinant ProteinsCarbon-Oxygen LyasesProtein Structure, TertiaryCalcium-Calmodulin-Dependent Protein KinasesPhosphoamino AcidsProtein Kinase CPhosphoproteinsTyrosineProtein-Tyrosine KinasesAmino Acid SubstitutionTransfectionEthers, CyclicEnzyme InhibitorsHydro-LyasesThreonine-tRNA LigaseValineCasein Kinase IIEscherichia coliProto-Oncogene Proteins c-aktRecombinant Fusion ProteinsGlycineProto-Oncogene ProteinsLysineGlycine Hydroxymethyltransferasep21-Activated KinasesProtein Processing, Post-TranslationalAspartate-Semialdehyde DehydrogenaseRNA, Transfer, ThrCOS CellsSequence AlignmentAlanineProto-Oncogene Proteins c-rafHeLa CellsMucinsCells, CulturedNutritional Requirements3T3 CellsLeucineElectrophoresis, Polyacrylamide GelModels, MolecularPrecipitin TestsGlycosylationDNA, ComplementaryAlcohol OxidoreductasesCatalytic DomainCatalysisStructure-Activity RelationshipBlotting, WesternMethionineAmino Acid MotifsPhosphotyrosineHomoserineDNA PrimersIsoenzymesGlycogen Synthase Kinase 3Amino Acids, EssentialProtein ConformationProtein Kinase InhibitorsPeptide FragmentsPhosphotransferasesPeptidesMitogen-Activated Protein KinasesPoint MutationConserved SequenceDeath-Associated Protein Kinases
SerineProteinPhosphorylationResiduesLysineReceptorPhosphataseAmino acidsIndispensable amino acidAlanineSubstrate specificityGeneConcentrationGeneticallyCrystal StructureBiosynthesisAssayRequirementSubunitDietaryDietsActivityObjectiveFamilyPatientsContentSequenceAccuratelyCellSpeciesConcentrationsAnalysisProstate cancerFunctionsPresentStructuresIndividual
Serine25
  • The protein is serine/threonine-rich. (wikipedia.org)
  • Phosphorylation of serine 1327 or threonine 1348 is unaltered. (jci.org)
  • Phosphorylation of receptor serine/threonine residues has been proposed to exert an inhibitory influence on receptor tyrosine kinase activity and Ser 1327 and Thr 1348 have been identified as specific sites of phosphorylation in the insulin receptor COOH terminal domain. (jci.org)
  • Moreover, within each group there was no correlation between the level of tyrosine kinase activity and the extent of serine/threonine phosphorylation. (jci.org)
  • It is concluded that the stoichiometry of serine/threonine phosphorylation of insulin receptor in vivo is low, and that increased phosphorylation of Ser 1327 or Thr 1348 is not responsible for the decreased insulin receptor tyrosine kinase activity observed in the skeletal muscle of NIDDM patients. (jci.org)
  • ATM serine/threonine kinase (ATM). (darkdaily.com)
  • The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. (spandidos-publications.com)
  • A corresponding class of transmembrane serine/threonine kinases has recently been discovered that act as cell surface receptors for ligands of the TGFβ superfamily. (biologists.com)
  • We assessed two of these genes-hepsin, a transmembrane serine protease, and pim-1, a serine/threonine kinase-at the protein level using tissue microarrays consisting of over 700 clinically stratified prostate-cancer specimens. (nature.com)
  • Cells treated with Calyculin A accumulate serine/threonine phosphorylation making them a strong positive control for phospho-threonine or phospho-serine specific antibodies. (abcam.com)
  • Work done using smooth muscle myosin and mammalian non-muscle myosin have demonstrated that phosphorylation of the RLC at conserved Serine and Threonine sites ( Figure 1B , Serine-19 and Threonine-18) activates myosin motor activity, enhances the affinity of myosin for actin, and promotes myosin filament assembly ( Heissler and Sellers, 2016 ). (elifesciences.org)
  • There are two types of glycosylation: N-linked (the glycan is attached to an asparagine) and O-linked (the glycan is attached to a serine or threonine). (neb.com)
  • A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine. (rcsb.org)
  • We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
  • The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)
  • Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
  • Enhanced activity of human N-myristoyltransferase by dimethyl sulfoxide and related solvents in the presence of serine/threonine-containing peptide substrates. (ageless.co.za)
  • Neuronal loss of the serine-threonine protein kinase Tao , a regulator of the Hippo signaling pathway, results in supernumerary boutons, each of which contain a normal number of active zones. (sdbonline.org)
  • They remove the phosphate group from both phospho-tyrosine and phospho-serine/threonine residues. (embl.de)
  • A competitive ELISA for quantitative measurement of Monkey Serine/threonine protein phosphatase PP1 β catalytic subunit(PPP1CB) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (pp1b.com)
  • Monkeys are generally considered to be intelligent, particularly Old World monkeys.Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. (pp1b.com)
  • however, TGF-β signalling also activates numerous serine/threonine kinases that phosphorylate Smad2 in its linker region. (iium.edu.my)
  • The expression of phosphorylated Smad linker proteins were determined following TGF-β stimulation in the absence and presence of different serine/threonine kinase inhibitors in vascular endothelial cells. (iium.edu.my)
  • Conclusions TGF-β-mediated phosphorylation of individual serine/threonine sites in the linker region of Smad2 occurs in a highly specific manner by kinases. (iium.edu.my)
  • Most (66 to 94%) cases appear to be caused by a germline mutation of the STK11/LKB1 (serine/threonine kinase 11) tumor suppressor gene. (msdmanuals.com)
Protein15
  • The phosphoinositide 3-kinase (PI3K)/RAC seine/threonine-protein kinase (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway is a promising therapeutic target owing to its frequent dysregulation in HCC and the critical functions it has in regulating cell survival, proliferation, apoptosis, migration and angiogenesis through phosphorylation of distinct protein substrates ( 6 , 7 ). (spandidos-publications.com)
  • Protein efficiency ratio and protein conversion efficiency values for fish fed the different diets also were significantly affected by threonine level and indicated requirement values of 0.8-0.9% of dry diet. (eurekamag.com)
  • Based on these data, the minimum threonine requirement of juvenile red drum was determined to be approximately 0.8% of dry diet (2.28% of dietary protein). (eurekamag.com)
  • The protein content was determined by BCA assay. (abcam.com)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • Processing soybean meal to increase the protein digestibility for better threonine absorption is recommended for its use as a feed additive. (celignis.com)
  • A study was conducted to determine if the crude protein or mineral content of a diet affected water consumption by growing pigs. (thepigsite.com)
  • To determine if lowering dietary crude protein (CP) or mineral level reduces drinking water consumption in growing pigs. (thepigsite.com)
  • Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
  • New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
  • Two major factors determine the formation of a protein kinase-substrate complex [ 4 ]. (biomedcentral.com)
  • However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
  • Raw and roasted pistachio nuts (Pistacia vera L.) are 'good' sources of protein based on their digestible indispensable amino acid score as determined in pigs. (nuthealth.org)
  • Studies suggest that the AKT3 protein plays a critical role in determining brain size. (medlineplus.gov)
  • Following exposure, SAEC showed time-dependent increases in reactive oxygen species production, total protein phospho-tyrosine and phospho-threonine levels, and migratory behavior. (cdc.gov)
Phosphorylation2
  • Phosphorylation of Ser 1327 and Thr 1348 was determined using antibodies that specifically recognize insulin receptor phosphorylated at these sites. (jci.org)
  • The method, named Predikin, identifies key conserved substrate-determining residues in the kinase catalytic domain that contact the substrate in the region of the phosphorylation site and so determine the sequence surrounding the phosphorylation site. (biomedcentral.com)
Residues3
  • However, a recent 134 base-pair PCR product was performed to determine report from the Artibonite Valley north of Port-au-Prince the amino acid haplotype of residues 72-76 as described documented that the P. falciparum chloroquine resistance ( 9 , 10 ). (cdc.gov)
  • The glycoprotein contains unique tandem repeats of eight amino acids, of which two threonine residues are probably glycosylated by N -acetyl-d-galactosamine (GalNAc). (figshare.com)
  • The heptapeptide sequence from -3 to +3 that best binds to the pocket is determined by the physicochemical nature of the residues in the catalytic domain that line the pocket and contact the substrate. (biomedcentral.com)
Lysine3
Receptor1
  • We examined the effect of amiloride on the activity of recombinant GABARs expressed transiently in L929 fibroblasts to determine its mechanism of action on mammalian GABARs and to determine whether the subunit composition of the receptor influenced its sensitivity to amiloride. (aspetjournals.org)
Phosphatase1
  • Here, the crystal structure of a human DSP, vaccinia H1-related phosphatase (or VHR), was determined at 2.1 angstrom resolution. (rcsb.org)
Amino acids1
Indispensable amino acid1
  • Threonine is an indispensable amino acid required by all animals for normal growth and metabolic functions. (eurekamag.com)
Alanine1
  • The concentrations of excitatory AAs (glutamate and aspartate) and inhibitory AAs (γ-aminobutyric acid and glycine) decreased and were stabilized by 45 minutes after probe implantation, whereas the levels of nonneurotransmitter AAs (alanine and threonine) were not stabilized at 60 minutes. (thejns.org)
Substrate specificity1
  • A "recognition region," connecting helix alpha1 to strand beta1, may determine differences in substrate specificity between VHR, the PTPs, and other DSPs. (rcsb.org)
Gene3
  • This study sought to determine the cellular and gene expression changes in small airway epithelial cells (SAEC) after in vitro exposure to MWCNT in an effort to elucidate potential toxicity mechanisms and signaling pathways. (cdc.gov)
  • We analyzed 16S rRNA gene amplicons to determine the principal environmental factors that shape the structure and function of microbial communities in shrimp cultural enclosure ecosystems from Guangdong and Hainan provinces, in China. (frontiersin.org)
  • Preliminary tests, such as BRAF mutation testing, may be useful in determining which patients are likely to benefit from full gene sequencing. (medscape.com)
Concentration1
  • For Glycine max a 21 d NOEC (shoot height) could not be determined as no concentration effect relationship occurred. (europa.eu)
Genetically2
  • Industrial production of threonine is achieved by fermentation using genetically engineered E. coli. (celignis.com)
  • [ 7 ] The tendency to develop pigmentation appears to be genetically determined, but color intensity might be influenced by smoking, hormones, systemic medications, and physical factors. (medscape.com)
Crystal Structure1
  • A crystal structure of Bck2 has not been determined. (wikipedia.org)
Biosynthesis1
  • Threonine is a crucial amino acid for intestinal health as it aids intestinal mucin biosynthesis and confers protection from intestinal damage. (celignis.com)
Assay7
Requirement3
  • An experiment was conducted in a brackish water (5 +- 1 ppt) recirculating system to quantify the minimum dietary threonine requirement of juvenile red drum Sciaenops ocellatus. (eurekamag.com)
  • Free threonine in plasma also significantly responded to increasing dietary threonine but indicated a slightly higher requirement value of approximately 1.0% of dry diet. (eurekamag.com)
  • As a feed additive, threonine is added to meet the amino acid requirement of livestock and helps to improve their weight. (celignis.com)
Subunit1
  • We measured its effects on recombinant GABARs to determine its mechanism of action at mammalian receptors and to examine the effect of subunit composition. (aspetjournals.org)
Dietary1
  • Weight gain and feed efficiency of fish fed the various diets were significantly (P lt 0.05) affected and increased linearly as dietary threonine increased until plateauing around 0.8% of dry diet. (eurekamag.com)
Diets2
  • Incremental levels of L-threonine were added to the diets and fed to juvenile red drum initially averaging 2.8 g/fish for 8 wk. (eurekamag.com)
  • Amino acid analysis of both foods was performed to determine that threonine was the limiting amino acid in both diets. (vin.com)
Activity1
  • Storage: store at 2-8°C Stability: The stability of kit is determined by the loss rate of activity. (pp1b.com)
Objective2
  • Introduction: Our objective was determining the optimal combinations of cerebrospinal fluid (CSF) biomarkers for predicting disease progression in Alzheimer's disease (AD) and other neurodegenerative diseases. (lu.se)
  • OBJECTIVE: Our goal was to determine participants' views of the acceptability and validity of the online trial methodology used in the pilot RCT of the Sexunzipped intervention. (who.int)
Family1
  • We extracted a novel glycoprotein, a member of the mucin family, from five species of jellyfish with high yields (1%−3% dry weight, 0.02%−0.1% wet weight) and determined its main chain structure and molecular mass. (figshare.com)
Patients1
  • GI cancer surveillance of Peutz-Jeghers syndrome patients includes colonoscopy, upper endoscopy, and video capsule endoscopy beginning at age 8, with the timing of subsequent surveillance determined by the findings. (msdmanuals.com)
Content1
  • We can determine the Threonine content of biomass, click here to learn more about our various biomass analysis methods. (celignis.com)
Sequence1
  • Alkylating agent and chromatin structure determine sequence context-dependent formation of alkylpurines. (ageless.co.za)
Accurately2
  • Faeces and urine were collected on days 11 to 14 of each period to allow us to accurately determine water intake, water output and the water:feed ratio. (thepigsite.com)
  • Although diagnosing dementia is largely a clinical endeavor, neuroimaging plays an increasingly important role in accurately determining the underlying etiology, which extends beyond its traditional role in excluding other causes of altered cognition. (medscape.com)
Cell1
  • Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
Species1
  • The chronic toxicity of Biofert Plusz to two terrestrial plant species was determined over a period of at 61 days (oats) and 43 days (turnip) according to DIN ISO 22030:2005. (europa.eu)
Concentrations2
  • optimal dilutions/concentrations should be determined by the end user. (abcam.com)
  • The AA concentrations in the dialysates were determined using high-performance liquid chromatography with fluorescence detection. (thejns.org)
Analysis1
  • Body mass and composition were determined by DEXA analysis. (vin.com)
Prostate cancer2
  • Thus, a research study that has identified unique genetic features within prostate cancer that can help determine if the cancer is aggressive or not, and whether certain drugs may be effective, is good news for men, for urologists, and for the clinical laboratories that will be called upon to perform testing. (darkdaily.com)
  • Signature expression profiles of normal adjacent prostate (NAP), BPH, localized prostate cancer, and metastatic, hormone-refractory prostate cancer were determined. (nature.com)
Functions2
  • Glycans are diverse, complex molecules and their functions are determined by their unique structures. (neb.com)
  • This study sought to determine whether the conserved Hippo pathway , critical for proportional growth in other tissues, also functions in NMJ development. (sdbonline.org)
Present1
Structures1
  • Here, we determined cryoelectron microscopy (cryo-EM) structures of Ric-8B in complex with Galphas and Galphaolf, revealing isoform differences in the relative positioning and contacts between the C-terminal alpha5 helix of Galpha within the concave pocket formed by Ric-8 alpha-helical repeat elements. (stanford.edu)
Individual1
  • Daily water intake (ADWI) and spillage was determined daily using individual water meters connected to bowl drinkers. (thepigsite.com)