SerinePhosphorylationThreoninePhosphoserineAmino Acid SequenceMolecular Sequence DataProtein-Serine-Threonine KinasesProtein KinasesCell LineTyrosinePhosphoproteinsEnzyme ActivationProtein Kinase CMutagenesis, Site-DirectedPeptide MappingPhosphothreonineBinding SitesPhosphopeptidesTransfectionKineticsSignal TransductionProtein BindingRecombinant ProteinsCells, CulturedSubstrate SpecificityMutationSequence Homology, Amino AcidBase SequenceProtein Structure, TertiaryCasein Kinase IIProtein-Tyrosine KinasesProtein Processing, Post-TranslationalElectrophoresis, Polyacrylamide GelPeptide FragmentsAmino AcidsAmino Acid SubstitutionModels, MolecularProto-Oncogene Proteins c-rafRecombinant Fusion ProteinsCOS CellsCyclic AMP-Dependent Protein KinasesSerine EndopeptidasesCloning, MolecularProtein ConformationMolecular WeightPhosphoprotein PhosphatasesPrecipitin TestsHeLa CellsDNA-Binding ProteinsPhosphotyrosineStructure-Activity RelationshipEnzyme InhibitorsPhosphoamino AcidsBlotting, WesternTetradecanoylphorbol AcetateSequence AlignmentCalcium-Calmodulin-Dependent Protein KinasesAmino Acid MotifsPeptidesProteinsEscherichia coliOxidative PhosphorylationCasein KinasesTumor Cells, CulturedOkadaic AcidProto-Oncogene Proteins c-aktTrypsinProto-Oncogene ProteinsAlanineMitogen-Activated Protein Kinases14-3-3 ProteinsMembrane ProteinsCattleCatalysisCell MembraneImmunoblottingImmunoprecipitationProtein Structure, SecondaryCatalytic DomainCarrier ProteinsMass SpectrometryCysteineIntracellular Signaling Peptides and ProteinsGlycogen Synthase Kinase 3IsoenzymesProtein Phosphatase 2DNA PrimersCricetinaeProtein TransportCell NucleusAdenosine TriphosphateCDC2 Protein KinaseCell Cycle ProteinsPoint MutationConserved SequenceCercopithecus aethiops3T3 CellsCalciumPhosphorus RadioisotopesModels, Biological