Threonine residue. Medical search. Frequent questions

The structure provides support for the importance of threonine and tyrosine residues in the signal transduction mechanism. (rcsb.org)
Protein phosphatases remove phosphate groups covalently attached to serine, threonine, and tyrosine residues. (rndsystems.com)
The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)

To examine the role of this conserved threonine residue in 5-HT 1A receptor coupling to G i /G o proteins, this residue was mutated to alanine (T149A mutant). (aspetjournals.org)
Processing of the precursor proteins exposes an N-terminal threonine residue that is the nucleophile in catalysis ( Aronson, 2004 ). (ebi.ac.uk)
can phosphorylate substrate proteins on serine, threonine, tyrosines or histidine residues. (horizondiscovery.com)
A group of enzymes that are dependent on cyclic GMP and catalyzes the phosphorylation of serine or threonine residues of proteins. (harvard.edu)
These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. (senescence.info)
In enzymatic assays, utilizing recombinant pol Î· (residues 1-432) proteins, the C34W, I147N, and R167Q variants showed 4- to 14-fold and 3- to 5-fold decreases in specificity constants (kcat/Km) for dATP insertion opposite the 3'-T and 5'-T of a CTD, respectively, compared to the wild-type, while the other variants displayed 2- to 4-fold increases. (bvsalud.org)

Sequence alignment of several G protein-coupled receptors identified a highly conserved threonine residue in the i2 loop of the 5-hydroxytryptamine 1A (5-HT 1A ) receptor that is a putative protein kinase C phosphorylation consensus site and is located in a predicted amphipathic α-helical domain. (aspetjournals.org)
Our analysis revealed that a highly conserved layer of threonine residues in the pore conveys a basal K+ selectivity. (uzh.ch)

O -linked glycans are most commonly attached to serine or threonine residues through the N -Acetylgalactosamine residue. (neb.com)

Moreover, a single mutation of threonine at position 350 to the corresponding methionine in the human counterpart abolished the repression of mCAR by steroid hormones. (aspetjournals.org)
Mutation of one of these residues, C215, results in mutant enzymes that are able to hydrolytically remove O-GlcNDAz from a model compound. (rsc.org)
Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)

HIPKs belong to the CMGC group of serine/threonine kinases and are part of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. (nature.com)
C-terminally adjacent to the HID follows a proline, glutamate, serine, and threonine (PEST)-rich domain, mediating proteasomal degradation of these kinases. (nature.com)
Mitogen-activated protein (MAP) kinases are serine/threonine kinases that mediate intracellular signal transduction pathways. (rcsb.org)
Mutagenesis data suggested a single residue difference at threonine 106 between p38 and other MAP kinases is sufficient to confer selectivity of pyridinyl imidazoles. (rcsb.org)
These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. (antibodies-online.com)
We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
In the second release includes also serine/threonine protein kinases, as well as an update of the tyrosine kinases. (lu.se)
Mutations appear both in conserved hallmark residues of the kinases as well as in non-homologous sites. (lu.se)

Finally, the C-terminus of HIPK1-3 comprises a region rich in serine, glutamine, and alanine (SQA) residues, which is involved in the interaction with different co-factors 14 . (nature.com)
We have changed the equivalent residue in human ERK2, Q105, into threonine and alanine, and substituted four additional ATP binding site residues. (rcsb.org)

This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. (origene.com)
The method, named Predikin, identifies key conserved substrate-determining residues in the kinase catalytic domain that contact the substrate in the region of the phosphorylation site and so determine the sequence surrounding the phosphorylation site. (biomedcentral.com)
The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
The heptapeptide sequence from -3 to +3 that best binds to the pocket is determined by the physicochemical nature of the residues in the catalytic domain that line the pocket and contact the substrate. (biomedcentral.com)

PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. (thermofisher.com)
Living up to its ominous acronym, RIPK1, the receptor-interacting serine/threonine-protein kinase 1 unleashes powerful signaling cascades that can fell cells within hours. (alzforum.org)
This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. (senescence.info)
New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)

Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. (senescence.info)

The β-linked N-acetyl-D-glucosamine (GlcNAc) is a post-translational modification of serine and threonine residues catalyzed by the enzyme O-GlcNAc transferase (OGT). (qub.ac.uk)

DYRKs are named after their characteristic dual-specificity, as they auto-phosphorylate a conserved tyrosine in their activation loop, but phosphorylate substrates on serine and threonine residues 2 . (nature.com)

describes the use of a phage-displayed combinatorial random peptide library to identify a 27-residue peptide that binds to C3 and inhibits complement activation. (justia.com)
This peptide was truncated to a 13-residue cyclic segment that maintained complete activity, which is referred to in the art as Compstatin. (justia.com)

Using homology modeling, we identified OGA residues proximal to the N -acyl position of O-GlcNAc substrate. (rsc.org)
This system consists of chymotrypsin residues 1-11 and 16-245, a three-residue substrate GAW in the active site, four sulfate ions, and 346 water molecules. (openmopac.net)

There is a signal sequence of 19 amino acid residues. (wikipedia.org)
The first 39 amino acids of the Dantu antigen are derived from glycophorin B and residues 40-99 are derived from glycophorin A. Dantu is associated with very weak s antigen, a protease-resistant N antigen and either very weak or no U antigen. (wikipedia.org)
There are three differences in the first three amino acid residues: the usual form has Tryptophan1-Serine-Threonine-Serine-Glycine5 while Henshaw has Leucine1-Serine-Threonine-Threonine-Glutamate5. (wikipedia.org)
All studies reporting universal chloroquine performed to amplify the P. falciparum pfcrt fragment susceptibility of P. falciparum parasites in Haiti were around amino acid residue 72-76. (cdc.gov)
However, a recent 134 base-pair PCR product was performed to determine report from the Artibonite Valley north of Port-au-Prince the amino acid haplotype of residues 72-76 as described documented that the P. falciparum chloroquine resistance ( 9 , 10 ). (cdc.gov)
The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)

STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. (nih.gov)

This posttranslational modification on serine or threonine residues is catalysed by the enzyme O-linked N-acetylglucosamine transferase (OGT) and shows increased occurrence in 4-MU treated MSC, presumably due to an overly high intracellular UDP-GlcNAc concentration. (europa.eu)

The mammalian O-GlcNAc hydrolase (OGA) removes O-GlcNAc from serine and threonine residues on intracellular glycoproteins. (rsc.org)

N -linked glycosylation occurs when glycans are attached to asparagine residues on the protein. (neb.com)

PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N -linked glycoproteins (5), as shown in Figure 2. (neb.com)
Other commonly used endoglycosidases such as Endoglycosidase H ( NEB #P0702 ) are not suitable for general deglycosylation of N -linked sugars, because it only deglycosylates glycoproteins containing primarily high mannose N -linked structures, as well as leaving one N -acetylglucosamine residue attached to the asparagine. (neb.com)

The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)

N-linked glycosylation occurs through the asparagine residues of the protein, while O-linked glycosylation occurs through serine or threonine. (neb.com)
Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. (senescence.info)

The antigenic determinant for the blood group Ss is located at residue 29 where S has a methionine and s a threonine. (wikipedia.org)

These residues occur in an active-like conformation stabilized by lanthanum ions. (rcsb.org)

Thus, threonine 350 seems to regulate hormone responsiveness of mCAR by interfering indirectly an interaction of the receptor with a coactivator. (aspetjournals.org)

They remove the phosphate group from both phospho-tyrosine and phospho-serine/threonine residues. (embl.de)

Based on the presence of glutamine or arginine residues in the zero layer, the SNAREs are classified as Qa- (Syntaxin-1), Qb- and Qc- (SNAP25) or R-SNAREs (Synaptobrevin-2) resulting in the formation of the ternary QabcR (3Q:1R) SNARE complex 8 . (nature.com)

They found it directly phosphorylated the protein at threonine-189, a residue that sits smack dab in the middle of the RIPK1 kinase domain. (alzforum.org)

The conserved i2 loop threonine may serve as a G protein contact site to direct the signaling specificity of multiple receptors. (aspetjournals.org)
PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. (thermofisher.com)

Residues 80-100 of glycophorin A and 51-71 of glycophorin B are very similar. (wikipedia.org)

The single residue change Q105A in ERK2 enhances the binding of SB202190 at least 25,000-fold compared to wild-type ERK2. (rcsb.org)

Tau hyperphosphorylation, mostly at serine (Ser) or threonine (Thr) residues, plays a key role in the pathogenesis of Alzheimer disease (AD) and other tauopathies. (nih.gov)

PNGase F digestion deaminates the asparagine residue to aspartic acid, leaving the oligosaccharide intact for further analysis. (neb.com)

Only O-glycosylation sites are found on glycoprotein B and these are linked via serine or threonine. (wikipedia.org)

The serine and threonine residues in the CT7n repeats are the target of such phosphorylation. (embl.de)

Anatomy18

Organisms14

Chemicals and Drugs133

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes57

Information Science4

Health Care3

Tyrosine residues3

Proteins6

Highly conserved2

Attached to serine1

Mutation3

Kinases10

Alanine2

Catalytic4

Protein kinase5

Serine Residue1

Catalyzed by the enzyme1

Phosphorylate1

Peptide2

Substrate2

Amino6

Substrates1

Enzyme1

Intracellular1

Glycans1

Glycoproteins2

Peptides1

Occurs2

Methionine1

Conformation1

Regulate1

Phosphate1

Glutamine1

Domain1

Loop2

Similar1

Single1

Role1

Analysis1

Found1

Target1