TyrosineMitosisSerineThreoninePhosphorylationAmino Acid SequenceMolecular Sequence DataMutagenesis, Site-DirectedBinding SitesCell LinePhosphoserineProtein BindingProtein-Serine-Threonine KinasesMutationProtein KinasesKineticsEnzyme ActivationPhosphoproteinsPhosphotyrosineProtein Structure, TertiaryBase SequenceSequence Homology, Amino AcidProtein Tyrosine PhosphatasesRecombinant ProteinsSignal TransductionModels, MolecularSubstrate SpecificityProtein-Tyrosine KinasesPeptide MappingProtein ConformationPhosphopeptidesAmino Acid SubstitutionTransfectionRecombinant Fusion ProteinsPeptide FragmentsStructure-Activity RelationshipCells, CulturedAmino AcidsTetranitromethaneProtein Processing, Post-TranslationalPhosphothreonineCloning, MolecularEnzyme InhibitorsCOS CellsSerine EndopeptidasesHeLa CellsElectrophoresis, Polyacrylamide GelProteinsSequence AlignmentProto-Oncogene ProteinsProtein Kinase CPhosphoprotein PhosphatasesBlotting, WesternAmino Acid MotifsEscherichia coliPrecipitin TestsTyrosine 3-MonooxygenaseDNA-Binding ProteinsPeptidesIntracellular Signaling Peptides and ProteinsCell Cycle ProteinsThreonine DehydrataseCDC2 Protein KinaseProtein Structure, SecondaryCyclic AMP-Dependent Protein KinasesMembrane ProteinsCasein Kinase IICysteineMolecular WeightCatalysisImmunoblottingAdaptor Proteins, Signal TransducingCell MembranePoint MutationCalcium-Calmodulin-Dependent Protein KinasesTrypsinCattleCatalytic DomainCarrier ProteinsMitogen-Activated Protein Kinases3T3 CellsCricetinaeAlanineConserved SequenceCell CycleCytoplasmDNA PrimersCell Nucleussrc Homology DomainsNuclear ProteinsDNAModels, BiologicalIsoenzymessrc-Family KinasesImmunoprecipitationTumor Cells, CulturedMutagenesisOkadaic AcidProtein TransportProto-Oncogene Proteins c-akt