• DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. (wikipedia.org)
  • The Bst X enzyme is similar to Bst polymerase in that it lacks 5'→3' and 3'→5' exonuclease activity and has a potent strand-displacement functionality. (qiagen.com)
  • Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus . (sigmaaldrich.com)
  • DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)
  • What is the activity of Thermostable USER® III Enzyme in other buffers? (neb.com)
  • FAQ: What is the activity of Thermostable USER ® III Enzyme in other buffers? (neb.com)
  • Thermostable USER III Enzyme is 100% active in Thermopol Buffer and has limited activity in other buffers. (neb.com)
  • Additionally, Thermolabile USER II Enzyme is 100% active in many polymerase and PCR buffers (see chart below). (neb.com)
  • At least one firm is designing molecular diagnostics around a thermostable enzyme discovered in an extremophile organism. (genomeweb.com)
  • The OmniAmp enzyme used by Lucigen is a polymerase with innate reverse transcriptase activity that was discovered in a virus inhabiting a Yellowstone National Park hot spring. (genomeweb.com)
  • This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb. (bio-rad.com)
  • iProof High-Fidelity DNA Polymerase is a unique Pyrococcus -like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates. (bio-rad.com)
  • The enzyme here referred to as Thermophi, has a C-terminal polymerase domain and a proofreading 3 ′ → 5 ′ exonuclease domain, but lacks the 5 ′ → 3 ′ exonuclease domain. (matis.is)
  • The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield. (thermofisher.com)
  • For superior PCR performance, we recommend the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase . (thermofisher.com)
  • Scientist often use a technique called X-ray crystallography to study intermediate structures of frozen polymerase crystals as the enzyme constructs DNA. (elifesciences.org)
  • used a particular method for making frozen polymerase crytals by allowing the enzyme to add new bases in liquid form. (elifesciences.org)
  • b) DNA polymerase is an enzyme involved in DNA replication. (mcqexams.com)
  • Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. (mcqexams.com)
  • Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand at 72$$^0$$C. (mcqexams.com)
  • This enzyme is able to withstand the protein-denaturing conditions (high temperature) required during PCR or it is thermostable. (mcqexams.com)
  • Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. (wazeesupperclub.com)
  • The new fragments of DNA that are made during PCR also serve as templates to which the DNA polymerase enzyme can attach and start making DNA. (wazeesupperclub.com)
  • Most enzyme-mediated labeling techniques are very much dependent on polymerase activity, which is responsible for incorporation of the labeled nucleotides. (enzolifesciences.com)
  • Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
  • Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
  • Bst X DNA Polymerase is provided with 10x Xcelerator Reaction Buffer (B7390) and 100 mM Magnesium Sulfate Solution. (qiagen.com)
  • Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl 2 (D1806) or a 10× reaction buffer without MgCl 2 plus a separate tube of MgCl 2 for titration (D4545). (sigmaaldrich.com)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase. (qiagen.com)
  • Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer. (thermofisher.com)
  • Platinum Taq Green Hot Start DNA Polymerase is provided with green PCR buffer that contains a density reagent and two tracking dyes and allows for direct gel loading of PCR products. (thermofisher.com)
  • Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. (thermofisher.com)
  • Lyo-Ready qPCR master mix is a glycerol-free formulation that contains reaction buffer, Taq (Thermostable DNA) polymerase, dNTP (deoxyribose nucleotide triphosphate), lyo-excipients, and MgCl2 (Magnesium chloride). (offshore-technology.com)
  • Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquaticus. (the-scientist.com)
  • A major breakthrough in DNA polymerase came along in the year 1969, when Thomas Brock reported the isolation of Thermus aquaticus , a new species of thermophilic bacterium found in the hot springs of Yellowstone National Park. (news-medical.net)
  • A novel and non-ubiquitous thermostable DNA polymerase in Thermus antranikianii was expressed in E. coli, isolated and biochemically characterized. (matis.is)
  • Secondly, DNA polymerases can only add new nucleotides to the preexisting strand through hydrogen bonding. (wikipedia.org)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
  • The main components consist of the Taq DNA polymerase, the template, the primers, and loose nucleotides in the form of deoxynucleoside triphosphates (dNTPs). (norgenbiotek.com)
  • DNA is composed of two complementary strands of linked nucleotides in matched pairs of A/T and C/G. Thus if one strand is present, the polymerase can produce the opposite strand. (norgenbiotek.com)
  • They are single nucleotides which the polymerase links together into the final product. (norgenbiotek.com)
  • Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing. (sigmaaldrich.com)
  • BIORONs unique thermostable SD Polymerase for Isothermal Amplification and regular PCR. (bioron.net)
  • It possesses heat stability up to 93 °C. With SD Polymerase possibilities of Isothermal Amplification and regular PCR can be combined. (bioron.net)
  • SD Polymerase can be used in various strand displacement reactions like LAMP or WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions. (bioron.net)
  • Although this technique used DNA polymerase repeatedly, similar to PCR, it employed only a single primer template complex, and exponential amplification was not possible using this technique. (news-medical.net)
  • Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. (thermofisher.com)
  • The amplification efficiency of HotVent exo- DNA Polymerase is 1 KB / min. (tinzyme.com)
  • How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)? (mcqexams.com)
  • High fidelity amplification using a thermostable DNA Pyrococcus Furiosus isolated polymerase. (taq-polymerase-dna.com)
  • The diagnosis of many infectious diseases, both viral and bacterial, may include the use of reverse transcriptase-polymerase chain reaction (RT-PCR). (medscape.com)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • New England Biosciences makes a similar kit, the WarmStart LAMP Kit for DNA and RNA, that uses a blend of Bst 2.0 DNA polymerase and RTx reverse transcriptase . (genomeweb.com)
  • In this dissertation, I begin with evaluating the usage of thermostable group II intron reverse transcriptase end-to-end template-switching activities in rapid preparations of strand-specific, low bias RNA sequencing libraries for the Illumina short-read sequencing platform. (utexas.edu)
  • Finally, I also established an efficient method to prepare high-quality single-stranded DNA sequencing libraries by exploiting the end-to-end template-switching activity and the DNA-dependent, DNA-polymerase activity of this reverse transcriptase and demonstrated its use in liquid biopsies. (utexas.edu)
  • Thermostable group II intron reverse transcriptase (TGIRTTM) enzymes and methods for their use are the subject of patents and patent applications that have been licensed by the University of Texas at Austin and East Tennessee State University to InGex, LLC. (cdc.gov)
  • DNA polymerases also cannot initiate DNA chains so they must be initiated by short RNA or DNA segments known as primers. (wikipedia.org)
  • The actual process of the PCR involves heating and cooling the mixture in order to sequentially separate the complementary strands, adhere the primers, and finally allow the polymerase to replicate the segment of interest. (norgenbiotek.com)
  • This technique sought to simplify gene synthesis by using artificial primers and templates that help DNA polymerase to copy the desired gene segments. (news-medical.net)
  • The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
  • This function is performed by DNA- directed DNA-polymerases ( EC 2.7.7.7 ) by adding nucleotide triphosphate (dNTP) residues to the 5'-end of the growing chain of DNA, using a complementary DNA chain as a template. (embl.de)
  • It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. (bjzblg.com)
  • A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29. (bjzblg.com)
  • Bst X DNA Polymerase is a recombinant, thermostable DNA polymerase from Geobacillus that is homologous to the well-known DNA polymerase from Bacillus stearothermophilus (Bst). (qiagen.com)
  • The protein is produced by a recombinant E. coli strain carrying the Bst X DNA Polymerase (exo-) polymerase gene. (qiagen.com)
  • Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary Platinum antibodies. (thermofisher.com)
  • Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene. (bjzblg.com)
  • One unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C. (qiagen.com)
  • Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. (sigmaaldrich.com)
  • The TAQXpedite PCR System contains a blend of thermostable DNA polymerases, an optimized 2X Universal MasterMix, and a Difficult/Long Master Mix with all four dNTPs. (genengnews.com)
  • and US No. 6,127,155, entitled "Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents. (genomeweb.com)
  • phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. (bjzblg.com)
  • During this process, the helix unwinds and enzymes called polymerases produce new strands (using the old ones as a template). (elifesciences.org)
  • is recommended for use in freeze drying applications as alternative to glycerol-free polymerase or in applications where only a minimal amount of volume can be used. (jenabioscience.com)
  • An increase in error rate with pH has also been noted for the exonuclease-deficient DNA polymerases Taq and exo- Klenow, suggesting that the parameters which influence replication error rates may be similar in pol l- and alpha-like polymerases. (neb.com)
  • DNA Polymerase I, Large (Klenow) Fragment is available at Gentaur for Next week delivery. (telospub.com)
  • A proteolytic product of E. coli DNA Polymerase I Biomolecule/Target: Alternates names: DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA. (telospub.com)
  • We report here expression of a truncated gene, encoding the copper A (Cu_A) domain of cytochrome ba_3, that is regulated by a T7 RNA polymerase promoter in Escherichia coli. (caltech.edu)
  • The polymerase is recommended for routine PCR applications (up to 4 kb fragment length) or high throughput PCR. (jenabioscience.com)
  • Crystal structures of an NH2-terminal fragment of T4 DNA polymerase and its complexes with single-stranded DNA and with divalent metal ions. (embl.de)
  • We report the crystal structure of an NH2-terminal 388-residue fragment of T4 DNA polymerase (protein N388) refined at 2.2 A resolution. (embl.de)
  • Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase. (wazeesupperclub.com)
  • The ligase might also be useful for RNA-mediated oligonucleotide annealing, selection, and ligation, or RASL-seq, Tripathi said, particularly because it is thermostable and template dependent. (genomeweb.com)
  • It displays both 5′ to 3′ polymerase and exonuclease activities. (sigmaaldrich.com)
  • The Expand High Fidelity PLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity. (sigmaaldrich.com)
  • The initial rate of specific activity of this polymerase on nicked DNA was about 360,000 U / mg protein. (matis.is)
  • The Cu_A-containing domain is purified in high yields as a water-soluble, thermostable, purple-colored protein. (caltech.edu)
  • Name the bacterium that yields a thermostable DNA polymerase. (mcqexams.com)
  • Carbohydrate modifying enzymes, Polysaccharides and microbial carbohydrate utilization (prebiotics, probiotics - food ingredients), Thermophiles and thermostable enzymes. (lu.se)
  • Three domains, often referred to as thumb, finger and palm domain work together to sustain DNA polymerase activity. (wikipedia.org)
  • It has both 5′→3′ polymerase and exonuclease activity. (sigmaaldrich.com)
  • The polymerase was stable at 70 ° C and lost 50% of its activity after 5 min at 85 ° C, but could be stabilized above 80 ° C by the addition of 0.5 M L-proline. (matis.is)
  • The antibodies dissociate during the initial PCR denaturation step and the DNA polymerase regains its full activity. (thermofisher.com)
  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity. (thermofisher.com)
  • A polymerase thermostable DNA which has an associated exonuclease activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus Furiosus (PFU). (taq-polymerase-dna.com)
  • Even though there are 2 different methods of priming, these are mediated by 2 very similar polymerases classes, A and B, with similar methods of chain elongation. (embl.de)
  • After a couple of failed attempts to isolate the Taq DNA polymerase, Susanne Stoffel and David Gelfand from Cetus finally isolated it in the fall of 1985, and Randy Saiki's experiments soon after proved that Taq polymerase is ideal for the PCR process. (news-medical.net)
  • The DNA polymerase from this bacterium, called the Taq Polymerase, could withstand very high temperatures, unlike other polymerases available at that point of time. (news-medical.net)
  • HotVent exo- DNA Polymerase purified E. coli strain of self weight group. (tinzyme.com)
  • The X-ray crystal structures of polymerase domains of DNA polymerases are described in analogy to human right hands. (wikipedia.org)
  • High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. (elifesciences.org)
  • Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. (elifesciences.org)
  • Taq Polymerase - high conc. (jenabioscience.com)
  • SD Polymerase is efficient for high GC-content and difficult targets. (bioron.net)
  • High-fidelity PCR reagents featuring the thermostable iProof Polymerase. (bio-rad.com)
  • The polymerase is a high fidelity heat-resistant DNA polymerase with a half-life of 8 hours at 100°C. It is suitable for primer extension and high temperature (72°C) DNA sequencing. (tinzyme.com)
  • This powerful polymerase mixture produces a high yield of PCR product from genomic DNA. (wazeesupperclub.com)
  • Increasing number of research and developmental activities, is one of the important factor which is responsible for the significant growth of high-fidelity DNA polymerase market. (factmr.com)
  • Other factors such as increasing number of research laboratories, Increasing awareness among the people, increasing technological advancements etc. is expected to increase the growth of high-fidelity DNA polymerase market. (factmr.com)
  • Introduction of government initiatives and increasing government funding for research and developmental activities, is expected to increase the growth of high-fidelity DNA polymerase market. (factmr.com)
  • Increasing occurrence of genetic diseases, is also one of the major factors increasing the growth of high - fidelity DNA polymerase market. (factmr.com)
  • Increasing number of people going for research activities and increasing scope of life sciences in academics, can also increase the growth of the high-fidelity DNA polymerase market. (factmr.com)
  • Apart from the factors increasing the growth of high-fidelity DNA polymerase market, there are few factors, which can hinder the overall growth of the high-fidelity DNA polymerase market. (factmr.com)
  • Lack of awareness in some of the research institutes, or lack of government funding in some of the developing regions, is expected to hinder the growth of high-fidelity DNA polymerase market. (factmr.com)
  • Based on the application type, the high-fidelity DNA polymerase market has been segmented into DNA cloning, SNAP Analysis, next generation sequencing applications and other applications. (factmr.com)
  • DNA cloning is expected to hold a large revenue share in the high-fidelity DNA polymerase market. (factmr.com)
  • Based on the end users, the high- fidelity DNA polymerase market has been segmented into research centers, academic institutions, biotechnology centers and others. (factmr.com)
  • Geographically, the global high-fidelity DNA polymerase market is segmented into key regions such as North America, Latin America, Europe, East Asia, South Asia, Oceania and MEA. (factmr.com)
  • North America is expected to be dominant market in the global high-fidelity DNA polymerase market because of the availability of all the healthcare facilities and presence of a well-established infrastructure in the field of healthcare. (factmr.com)
  • Increasing number of research institutes is another important factor, which can increase the growth of high-fidelity DNA polymerase market. (factmr.com)
  • Europe is expected to hold a large revenue share in the global high-fidelity DNA polymerase market throughout the forecast period because of the introduction of new research equipment and new research products in the field of healthcare industry. (factmr.com)
  • There are several key players in the high-fidelity DNA polymerase market. (factmr.com)
  • Examples of some of the key players operating in the global High-fidelity DNA polymerase market are Thermo Fisher Scientific, Inc., Bio-Rad Laboratories, Inc., Merck KGaA, Jena Bioscience GmbH, Agilent Technologies, Inc., New England Biolabs. (factmr.com)
  • The fidelity of a DNA polymerase refers to its ability to accurately replicate a template. (factmr.com)
  • In the year 1977, Frederick Sanger identified a DNA sequencing method involving a DNA polymerase, a primer, and nucleotide precursors, for which he was he awarded the Nobel Prize in 1980. (news-medical.net)
  • The replication fidelities of Pfu, Taq, Vent, Deep Vent and UlTma DNA polymerases were compared using a PCR-based forward mutation assay. (neb.com)
  • Name the source organism that possesses Taq polymerase. (mcqexams.com)
  • T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. (bjzblg.com)
  • The error rates of a Taq/Pfu DNA polymerase mixture and a Klentaq/Pfu DNA polymerase mixture were found to be less than the error rate of Taq DNA polymerase, but approximately 3-4-fold higher than the error rate of Pfu DNA polymerase. (neb.com)
  • There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. (the-scientist.com)
  • First of all, all DNA polymerases must have both a template strand and a primer strand. (wikipedia.org)
  • Unlike RNA, DNA polymerases cannot synthesize DNA from a template strand. (wikipedia.org)
  • A critical aspect of this is the ability of the DNA polymerase to read a template strand, select the appropriate nucleoside triphosphate and insert the correct nucleotide at the 3′ primer terminus, such that canonical Watson-Crick base pairing is maintained. (factmr.com)
  • The Bst X DNA Polymerase (40,000 U) is intended for molecular biology applications. (qiagen.com)
  • E. coli bacteria produces 5 different DNA polymerases: DNA Pol I, DNA Pol II, DNA Pol III, DNA Pol IV, and DNA Pol V. Eukaryotic cells contain 5 different DNA polymerases: α, β, γ, δ, and ε. (wikipedia.org)