This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies. (beilstein-journals.org)
Acyl1
The expression of regulatory molecules, TGF-β1/2, phospho-Akt (Ser473), PPARα, sterol regulatory element-binding protein 1 (SREBP-1), fatty acid synthase (FASN), hormone-sensitive lipase (HSL), and acyl dehydrogenases was analyzed in virus-infected hepatocytes. (academic-accelerator.com)
Significantly2
The expression levels of LPL (lipoprotein lipase), HSL (Hormone-sensitive lipase), FAS (fatty acid synthase), and PPARα/PPARγ (peroxisome proliferator-activated receptor alpha/gamma) were significantly changed in the sheep preadipocytes during differentiation for 7 days. (academic-accelerator.com)
Lipases and phospholipases are also used to improve dough tolerance, significantly increasing bread volume after baking. (kerry.com)
Bacillus1
The structure of Bacillus stearothermophilus P1 lipase provides a template for other thermostable lipases, and offers insight into mechanisms used to enhance thermal stability which may be of commercial value in engineering lipases for industrial uses. (inrae.fr)
Enzyme6
It includes lipases from gram positive bacteria, organic-solvent tolerant, showing thermoalkalophilic properties and high molecular weight resulting from extra domain where a zinc ion is coordinatively bound to the enzyme. (inrae.fr)
Moreover, the classification enables us to predict (1) important structural features such as residues forming the catalytic site or the presence of disulphide bonds, (2) types of secretion mechanism and requirement for lipase-specific foldases, and (3) the potential relationship to other enzyme families. (inrae.fr)
The optimum temperature and pH for immobilized enzyme activity were found to be 50 o C and 8.0 respectively. (innspub.net)
The immobilized enzyme exhibited its stability at much wider alkaline pH ranges and higher temperatures even after 24 hours incubation. (innspub.net)
Km and Vmax values of the immobilized enzyme were 6.618 mg/ml and 131.578 µmol/min/mg of protein respectively. (innspub.net)
The agar-agarose immobilized enzyme could be reused up to 5 repeated cycles and it is stable for at least 1 month when stored at 4 o C. Hence this immobilized cellulase with good storage stability than the soluble one can be considered for commercial applications. (innspub.net)
Aspergillus1
Optimization of Aspergillus hortai Cellulase Immobilized on Poly ( Acrylamide-Co-Acrylic Acid ) for Hydroxylation of Cellulose Rice Straw Wastes. (innspub.net)
Heterologous2
Gargouri A. (2013) Cloning and heterologous expression of a thermostable pectate lyase from Penicillium occitanis in Escherichia coli. (cbs.rnrt.tn)
After the successful validation of DNA transfer and positive selection in M. thermautotrophicus ΔH, we demonstrated heterologous gene expression of a thermostable ß-galactosidase-encoding gene (bgaB) from Geobacillus stearothermophilus under the expression control of four distinct synthetic and native promoters. (bvsalud.org)
Bacterial1
Here we propose an updated and extensive classification of bacterial esterases and lipases based mainly on a comparison of their amino acid sequences and some fundamental biological properties. (inrae.fr)
Synthesis1
Furthermore, novel biotechnological applications have been successfully established using lipases for the synthesis of biopolymers and biodiesel, the production of enantiopure pharmaceuticals, agrochemicals, and flavour compounds. (inrae.fr)
Enzymes1
Both free and immobilized forms of enzymes were inhibited significantly by Hg 2+ metal ion and the activity of the latter was affected in the presence of detergents and additives at higher concentration. (innspub.net)
Geobacillus1
The purpose of this study was to produce cross-linked enzymatic aggregates (CLEAs) of LipMatCCR11, a 43 kDa recombinant solvent-tolerant thermoalkaliphilic lipase from Geobacillus thermoleovorans CCR11. (bvsalud.org)
Stability1
The structure contains a zinc-binding site which is unique among all lipases with known structures, and which may play a role in enhancing thermal stability. (inrae.fr)
Properties3
The optimisation of industrially relevant lipase properties can be achieved by directed evolution. (inrae.fr)
In this study, purified thermostable cellulase from Stenotrophomonas maltophilia was immobilized on an agar-agarose matrix, and the properties of immobilized cellulase were studied. (innspub.net)
Urease immobilized on chitosan membrane: Preparation and properties. (innspub.net)
Family1
Abdeljalil S., Trigui-Lahiani H., Lazzez H., Gargouri A. (2013) Cloning, molecular characterization, and mRNA expression of the thermostable family 3 β-glucosidase from the rare fungus Stachybotrys microspora. (cbs.rnrt.tn)
Production2
The high-level production of microbial lipases requires not only the efficient overexpression of the corresponding genes but also a detailed understanding of the molecular mechanisms governing their folding and secretion. (inrae.fr)
Bouzid M., Gargouri A., Belghith H. (2013) Application of a Chitosan-Immobilized Talaromyces thermophilus Lipase to a Batch Biodiesel Production from Waste Frying Oils. (cbs.rnrt.tn)