• Lipozymes can be differentiated by origin - it can be extracted from Mucor miehei, Thermomyces lanuginosus, Candida antarctica, and others. (wikipedia.org)
  • The zeolitic materials were used as a solid supports for the immobilization of fungal Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL) lipases and the catalytic potential of these nanozeolite-enzyme complexes were explored in the transesterification reactions of oil palm oil and microalgae aiming the biodiesel production for biodiesel production using ethanol as solvent. (unesp.br)
  • Further, utilizing different lipase specificities, addition of Thermomyces lanuginosus lipase (TLL), allowed the CALA product mixture to be cleaned up by hydrolyzing attached glycerol. (mdpi.com)
  • In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). (researchgate.net)
  • In this paper, we describe a simple alternative enzymatic process, in which castor oil is directly converted to an estolide mixture by Candida antarctica lipase A (CALA) catalyzed transesterification. (mdpi.com)
  • Lipozyme, a registered trademark of Novo Nordisk A/S Corp., is a class of industrial enzymes, specifically: lipases. (wikipedia.org)
  • Other enzymes and lipases from other microorganisms were also tested in the biocatalytic esterification of mono-and disaccharides. (researchgate.net)
  • The results have indicated the nature and concentration of the divalent cations solutions have played a significant role and strongly affected the amount and the enzymatic activity of the immobilized enzymes. (unesp.br)
  • Lipases like Lipozyme and Novozyme (reg.trademark by Novozymes) play a big role in the synthesis of biodiesel. (wikipedia.org)
  • Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
  • Recently, reports have emerged detailing lipase-catalyzed synthesis of estolides from free hydroxy fatty acids. (mdpi.com)
  • The modification process imparted switchable wettability to the surface while maintaining the thermal stability and biocompatibility of the CF. During the ethanol transformation, the rapid, reversible cell adsorption and detachment of Saccharomyces cerevisiae were performed through the modulation of wettability, displaying the enhancement of immobilized biomass and immobilization efficiency from 2.20 g/L and 59.43% to 2.81 g/L and 93.32%, respectively. (edu.au)
  • With a very high molar proportion of fatty acid to saccharide when compared to the other studies, the authors suggested that increasing the proportion of fatty acid could negatively affect the lipase conformation due to an acidification of the reaction medium. (researchgate.net)
  • [11] have reported a lipase-mediated acylation of an equimolecular mixture of D/L-thymidine with acetonoxime levulinate as acylating agent and Pseudomonas cepacia lipase as biocatalyst. (beilstein-journals.org)
  • The reaction mixture is analyzed by NMR to determine the estolide number (EN) and MALDI MS to identify individual components, in addition to titration to determine the acid value (AV). Estolide trimers and tetramers (EN 2-3) were formed over 24 h in a system with 2:1 ( v/v ) castor oil-water. (mdpi.com)
  • Free and immobilised lipases were tested for their esterification activities. (researchgate.net)
  • The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • Based on the accumulation of the biofilm, the fabrication and utilization of PNIPAM-CF improved the efficiency of continuous immobilized fermentation, making the ethanol production reach 26.34 g/L in the sixth batch of fermentation. (edu.au)
  • Simplex lattice mixture design was employed to evaluate the effect of the composition of lipase mixtures on the yield of transesterification reaction. (uludag.edu.tr)
  • Lipase-mediated catalysis has been undertaken with reagent-based solvents and lyophilized powders, but there are no examples of molecularly dispersed enzymes that catalyse reactions at sub-solvation levels within solvent-free melts. (nature.com)
  • In aqueous environments, lipases catalyse the hydrolysis of fatty acid esters by a two-step mechanism that involves nucleophilic attack by a serine residue located within the active site to produce an acylated enzyme and alcohol product, followed by nucleophilic addition of a water molecule to liberate the fatty acid 1 . (nature.com)
  • The lipases comprise an active site based around a serine-histidine-aspartate triad (Ser144-His257-Asp203 for RML, Ser146-His258-Asp201 for TLL) with substrate access regulated by conformational changes involving a helical 'lid' motif ( Fig. 1a ), and which together facilitate nucleophilic attack on the fatty acid ester substrate to produce the acylated enzyme and alcohol product ( Fig. 1b ). (nature.com)
  • Cross-linked enzyme aggregates (CLEAs) present several interesting advantages over carrier-bound immobilized enzymes, such as highly concentrated enzymatic activity, high stability of the produced superstructure, important production costs savings by the absence of a support, and the fact that no previous purification of the enzyme is needed. (conicet.gov.ar)
  • Protein surface clusters, more probable configurations of the protein-supports systems at immobilization pHs, immobilized enzyme activity, and protein load were predicted by RDID1.0 software. (bvsalud.org)
  • Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. (ugm.ac.id)
  • In the current contribution the described protocol was applied to CLEAs of Thermomyces lanuginosa lipase, which showed a recovered specific activity of 11.1% relative to native lipase. (conicet.gov.ar)