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  • Ligand
  • Biotin binds tightly to the tetrameric protein avidin (also streptavidin and neutravidin), with a dissociation constant Kd on the order of 10−15 M, which is one of the strongest known protein-ligand interactions. (wikipedia.org)
  • interactions
  • The guide also contains a discussion on various applications where crosslinking has been applied, including the powerful label-transfer technique for identifying or confirming protein interactions. (thermofisher.com)
  • Chromatin immunoprecipitation (ChIP) is a common technique for studying epigenetics, as it allows the researcher to capture a snapshot of specific protein-DNA interactions. (thermofisher.com)
  • With the advent of next-generation sequencing (NGS), ChIP has become even more powerful-researchers can now get a snapshot of not only specific protein- DNA interactions in a few regions but also genome-wide interactions, by adapting purified ChIP DNA for NGS, called ChIP-sequencing (ChIP-Seq). (thermofisher.com)
  • The attachment of biotin to various molecules, biotinylation, is used as an important laboratory technique to study various processes, including protein localization, protein interactions, DNA transcription, and replication. (wikipedia.org)
  • Today, organic chemists utilize NMR for mapping protein interactions or identifying small compounds, but NMR was first a passion project of physicists. (wikipedia.org)
  • fluorescent
  • Most fluorophores are organic small molecules of 20 - 100 atoms (200 - 1000 Dalton - the molecular weight may be higher depending on grafted modifications, and conjugated molecules), but there are also much larger natural fluorophores that are proteins: Green fluorescent protein (GFP) is 27 kDa and several phycobiliproteins (PE, APC. (wikipedia.org)
  • fluorescent proteins GFP (green), YFP (yellow) and RFP (red) can be attached to other specific proteins to form a fusion protein, synthesized in cells after transfection of a suitable plasmid carrier. (wikipedia.org)
  • synthesis
  • Biotin is an important component of enzymes involved in metabolizing fats and carbohydrates, influencing cell growth, and affecting amino acids involved in protein synthesis. (wikipedia.org)
  • transmembrane
  • BMPR1A is one of the bone morphogenetic protein (BMP) receptors which are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. (thermofisher.com)
  • organic
  • Based on molecular complexity and synthetic methods, fluorophore molecules could be generally classified into four categories: proteins and peptides, small organic compounds, synthetic oligomers and polymers, and multi-component systems. (wikipedia.org)
  • reaction
  • Because there are significant differences in molecular weights of tested carrier proteins, this presentation is more relevant to practical uses than the mole-to-mole comparison (i.e., conjugation protocols are alike in specifying a given milligram amount of carrier protein for reaction regardless of its molecular weight). (thermofisher.com)
  • Purity
  • To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein. (thermofisher.com)
  • biological
  • Hydrogen isotope biogeochemistry is the scientific study of biological, geological, and chemical processes in the environment using the distribution and relative abundance of hydrogen isotopes. (wikipedia.org)
  • popular
  • However, NHS esters and imidoesters are the most popular amine-specific functional groups that are incorporated into reagents for protein crosslinking and labeling. (thermofisher.com)
  • crystals
  • In response to some of this data, NASA wants to investigate microgravity's effects on the growth of three-dimensional, human-like tissues, and the unusual protein crystals that can be formed in space. (wikipedia.org)
  • Many scientific techniques exploit NMR phenomena to study molecular physics, crystals, and non-crystalline materials through nuclear magnetic resonance spectroscopy. (wikipedia.org)
  • form
  • The CCH protein is composed of two very large polypeptide subunits (404/351 kDa) that form an extremely stable heterodidecameric structure even in the absence of divalent cations. (thermofisher.com)
  • Kevlar's structure consists of relatively rigid molecules which tend to form mostly planar sheet-like structures rather like silk protein. (wikipedia.org)
  • ELISA
  • Our expertise helps increase the probability that you will obtain a polyclonal or monoclonal antibody capable of distinguishing among even highly related proteins within complex mixtures to characterize protein expression patterns using IHC, ICC, IF, FACS, ELISA, western blotting, or immunoprecipitation (IP, Co-IP, ChIP). (thermofisher.com)
  • molecular
  • Transient expression in the cells of five infectious molecular clones of BLV with a single alanine mutation at five common antibody binding sites had no effect syncytia formation of the gp51 protein. (biomedcentral.com)
  • surfaces
  • therefore, they occur predominantly on the outside surfaces of native protein tertiary structures where they are readily accessible to conjugation reagents introduced into the aqueous medium. (thermofisher.com)
  • ensure
  • For example, if you are interested in knowing where H3K9me2 protein is bound on the genome, you want to ensure that you are pulling down only the dimethyl (me2) mark and not the monomethyl (me1) or trimethyl (me3) mark. (thermofisher.com)
  • cell
  • The Fuse-It liposomal carrier, which includes the siRNA, simply fuses with the cell membrane and then releases the siRNA directly into the cytoplasm. (ibidi.com)
  • products
  • Although grown worldwide for its protein and oil, high value added products such as plant functional nutraceuticals, including phospholipids, saponins, isoflavones, oligosaccharides and edible fibre, have gained importance in the last decade. (intechopen.com)
  • Great
  • In this existing download of rate in the Magnitude of Stolen Innocence, the function of Warren Jeffs, the interested Prophet of the FLDS Church, is you great inside the few education eligible German carrier Based by her mix and how she had it. (onairsol.com)
  • include
  • Methods of vaccinating to raise an immune response include the administration of a protein or polypeptide which stimulates an aspect of the immune response in vivo, as well as the administration of a polynucleotide which, once properly administered to a subject, is transcribed and translated into a protein or polypeptide which stimulates an aspect of the immune response in vivo. (google.com)
  • When completed, The ISS will include a number of modules devoted to scientific activity as well as other hardware designed for the same purpose. (wikipedia.org)
  • results
  • Content: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. (aaccjnls.org)
  • Consequently, there is the real danger of the scientific literature being corrupted with a multitude of publications reporting inadequate and conflicting results( 13 ). (aaccjnls.org)