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  • cytokine expression
  • Appropriate immune responses to particular pathogens are orchestrated by distinct T cell populations with relatively stable cytokine expression profiles that differentiate during antigen-specific clonal proliferation. (rupress.org)
  • regulate
  • Cooperatively bound BATF and IRF4 TFs in T H 17-polarized cells contributed to initial chromatin accessibility and subsequent recruitment of the nuclear receptor RORγt to regulate a selection of T H 17-relevant genes. (sciencemag.org)
  • Here we used transcriptional profiling of developing TH17 cells to construct a model of their signalling network and nominate major nodes that regulate TH17 development. (broadinstitute.org)
  • In this study, we found that small intestinal eosinophils down-regulate Th17 cells. (rupress.org)
  • Further understanding on how Smads, in cooperation with distinct transcription factors, regulate Th17 cell generation is needed for the potential development of therapeutic strategies targeting this pathway. (rupress.org)
  • Foxp3(+) T regulatory (Treg) cells regulate immune responses and maintain self-tolerance. (nih.gov)
  • (rupress.org)
  • In human purified naive T cells, PGE2 acts via prostaglandin receptor EP2- and EP4-mediated signaling and cyclic AMP pathways to up-regulate IL-23 and IL-1 receptor expression. (sigmaaldrich.com)
  • mice
  • Here we report the identification of the ligand-activated transcription factor aryl hydrocarbon receptor (AHR) as a regulator of T reg and T H 17 cell differentiation in mice. (nature.com)
  • On the other hand, AHR activation by 6-formylindolo[3,2-b]carbazole interfered with T reg cell development, boosted T H 17 cell differentiation and increased the severity of experimental autoimmune encephalomyelitis in mice. (nature.com)
  • Using a mouse model of MS, the researchers deleted the Satb1 gene in Th17 cells and found that the disease no longer developed in the mutant mice. (news-medical.net)
  • Intrapancreatic injections of adenovirus encoding IL-17A, the main cytokine produced by TH17 cells, into transgenic mice after KrasG12D activation led to an increase in the progression of PanIN lesions by 6 weeks when compared with mice injected with luciferase-adenovirus. (aacrjournals.org)
  • Taz fl/fl Lck -Cre mice) immunized with KLH exhibited a larger T H 17 population and fewer T reg cells than that of their Taz fl/fl littermates. (nature.com)
  • Here, we have reported that miR-146a-deficient mice developed more severe experimental autoimmune encephalomyelitis (EAE), an animal model of human multiple sclerosis (MS). We bred miR-146a-deficient mice with 2D2 T cell receptor-Tg mice to generate 2D2 CD4 T cells that are deficient in miR-146a and specific for myelin oligodendrocyte glycoprotein (MOG), an autoantigen in the EAE model. (jci.org)
  • miR-146a-deficient mice develop more severe experimental EAE featuring exaggerated Th17 responses against autoantigen. (jci.org)
  • E ) ELISA analysis of cytokine production by splenic cells harvested from day-28 EAE mice and stimulated with MOG 35-55 . (jci.org)
  • F ) Representative FACS plots showing the intracellular IL-17A staining of spinal cord-infiltrating lymphocytes (pregated on TCRβ + CD4 + cells) harvested from day-18 EAE mice ( n = 3). (jci.org)
  • H ) qPCR analysis of miR-146a expression in spinal cord-infiltrating CD4 + T cells harvested from WT mice at the indicated time points after EAE induction. (jci.org)
  • Mice with a conventional knockout, as well as a T cell-specific deletion, of the Irf8 gene exhibited more efficient T\(_H\)17 cells. (harvard.edu)
  • Th17 cells in the small intestine were markedly increased in the ΔdblGATA-1 mice lacking eosinophils, and an inverse correlation was observed between the number of eosinophils and that of Th17 cells in the small intestine of wild-type mice. (rupress.org)
  • showed that compared with WT counterparts in the same recipient mice, Mink1-deficient cells displayed enhanced IL-17 production and expression of the activation marker CD44 and reduced expression of the lymph node homing receptor CD62L, which is highly expressed in naive cells. (rupress.org)
  • A) CD4 + T cells were purified from Foxp3-GFP.KI mice and the Foxp3 + and Foxp3cells were sorted. (nih.gov)
  • B, C) Naïve CD4 + CD62L + T cells were sorted from WT, Tigit −/− (B) or Tigit tg (C) mice and differentiated as in (A). Foxp3 expression was analyzed by flow cytometry. (nih.gov)
  • D) CD4 + Foxp3 + TIGIT + (◆) or CD4 + Foxp3 + TIGIT − (◇) Treg cells were sorted from Foxp3-GFP.KI mice and titrated onto Foxp3 − GFP − effector T cells stimulated with anti-CD3 and APCs. (nih.gov)
  • For instance, Th17 cells appear to have a predominant role in antifungal immune responses in both humans and mice ( 14 - 16 ). (rupress.org)
  • Lamacchia C, Palmer G, Seemayer CA, Talabot-Ayer D, Gabay C. Enhanced Th1 and Th17 responses and arthritis severity in mice with a deficiency of myeloid cell-specific interleukin-1 receptor antagonist. (springer.com)
  • However, mice with STAT3-deficient T cells, which are unable to differentiate into Th17 cells, rejected lung allografts and developed OB similar to control mice. (sigmaaldrich.com)
  • The frequency of IL-17A(+) cells was not decreased in mice with STAT3-deficient T cells due mainly to the presence of IL-17A(+) γδ T cells. (sigmaaldrich.com)
  • Consequently, mice that lack BCAP in T cells had reduced susceptibility to experimental autoimmune encephalomyelitis. (rupress.org)
  • Surprisingly, BDC2.5(+) cells recovered from diabetic NOD.scid mice, in comparison with those from neonate NOD mice, showed predominant IFN-gamma over IL-17 expression, indicating conversion of donor cells into Th1 cells. (nih.gov)
  • C. Averages of the percentage of IL-17 or IFN-γ positive cells gated from the CD4+Tcr-β+ cells (n= 6-8 mice per group analyzed in independent experiments). (nih.gov)
  • A. CD4+ from BDC2.5 mice were differentiated to Th17, shown is the representative cytokine profile on day 3 after activation. (nih.gov)
  • B. Diabetes incidence when 1 million Th17 cells from A) were injected into NOD.scid mice. (nih.gov)
  • C. Diabetes incidence when different doses of Th17 cells were transferred into NOD.scid mice. (nih.gov)
  • D. Photograph of pancreas of NOD.scid mice that received Th17 cells sections. (nih.gov)
  • E. Diabetes incidence when 1 million differentiated BDC2.5 Th17 cells were injected into 7-day-old NOD mice (Results derived from two independent experiments). (nih.gov)
  • FoxP3
  • Regulatory T cells (T reg ) expressing the transcription factor Foxp3 control the autoreactive components of the immune system. (nature.com)
  • The balance between Th1, Th17, and FoxP3 + CD4 + T cell subsets in sarcoidosis remains unclear. (hindawi.com)
  • Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases, and 15 healthy controls, were investigated with flow cytometry for intracellular expression of FoxP3. (hindawi.com)
  • In addition, TAZ attenuated T reg cell development by decreasing acetylation of the T reg cell master regulator Foxp3 mediated by the histone acetyltransferase Tip60, which targeted Foxp3 for proteasomal degradation. (nature.com)
  • In contrast, under T reg cell-skewing conditions, TEAD1 expression and sequestration of TAZ from the transcription factors RORγt and Foxp3 promoted T reg cell differentiation. (nature.com)
  • In this study, we show that enhanced activity of Stat3 in CD4(+)CD45RA(-)Foxp3(-) and Foxp3(low) effector T cells from children with LN correlates with increased frequencies of IL-17-producing cells within these T cell populations. (sigmaaldrich.com)
  • Interestingly, murine Th17 cells share a common origin with Foxp3 + T regulatory cells, because both populations are produced in response to transforming growth factor-β, but they develop into Th17 cells only when IL-6 is simultaneously produced. (biomedcentral.com)
  • Foxp3 + nTreg cells were stained directly for TIGIT (solid line) or with an isotype control (dotted line) and analyzed by flow cytometry. (nih.gov)
  • Foxp3 + induced Treg (iTreg) cells were analyzed after 4 days of stimulation with TGF-β. (nih.gov)
  • Immunity
  • Diverse targets of the transcription factor STAT3 contribute to T cell pathogenicity and homeostasis," Immunity , vol. 32, no. 5, pp. 605-615, 2010. (hindawi.com)
  • T helper 17 cells promote cytotoxic T cell activation in tumor immunity," Immunity , vol. 31, no. 5, pp. 787-798, 2009. (hindawi.com)
  • 2011) demonstrate in this issue of Immunity that autocrine TGF-β cytokine promotes Th17 cell development and maintenance. (biomedsearch.com)
  • The discovery of these novel Th17 cell-mediated direct protective and indirect helper effects important for intracellular immunity highlights the diversity of Th17 cell roles, and increases understanding of protective T. cruzi immunity, aiding the development of therapeutics and vaccines for Chagas disease. (sigmaaldrich.com)
  • Th17 cells are positioned at a crossroads between innate and adaptive immunity and provide mediators that are essential for host defense. (springer.com)
  • transcriptional
  • Computational and experimental analyses help us understand the T H 17 cell transcriptional regulatory network. (sciencemag.org)
  • These findings define IRF8 as a novel intrinsic transcriptional inhibitor of T\(_H\)17-cell differentiation. (harvard.edu)
  • T helper [Th]17) cells in vivo and in vitro due to a failure to express sufficient levels of the Th17-specific transcriptional regulator retinoid-related orphan receptor γt. (rupress.org)
  • Such differentiation of CD4 + T cells is determined by signals provided by components of the innate immune system and is subsequently stabilized by induction and repression of key transcriptional regulators ( 1 ). (rupress.org)
  • type 17
  • Proinflammatory T helper type 17 cells are effective B-cell helpers," Proceedings of the National Academy of Sciences of the United States of America , vol. 107, no. 32, pp. 14292-14297, 2010. (hindawi.com)
  • regulator
  • https://doi.org/10.1084/jem.20161120 ) identified the kinase Mink1 as a novel negative regulator of Th17 cell generation. (rupress.org)
  • lineages
  • Although T reg cell dysfunction and/or T H 17 cell dysregulation are thought to contribute to the development of autoimmune disorders, little is known about the physiological pathways that control the generation of these cell lineages. (nature.com)
  • pathogenesis
  • Th17 cells infiltrate the kidneys of patients with lupus nephritis (LN) and are critical for the pathogenesis of this disease. (sigmaaldrich.com)
  • In contrast, it is unlikely that Th17 cells contribute to the pathogenesis of human allergic IgE-mediated disorders, because IL-4 and IL-25 (a powerful inducer of IL-4) are both potent inhibitors of Th17 cell development. (biomedcentral.com)
  • interferon
  • Preferential recruitment of interferon- γ -expressing TH17 cells in multiple sclerosis," Annals of Neurology , vol. 66, no. 3, pp. 390-402, 2009. (hindawi.com)
  • Moreover, the presence in the circulation and in various tissues of Th cells that can produce both IL-17 and interferon-γ, as well as the flexibility of human Th17 clones to produce interferon-γ in addition to IL-17 in response to IL-12, suggests that there may be a developmental relationship between Th17 and Th1 cells, at least in humans. (biomedcentral.com)
  • Transcription Factors
  • Here we found that TAZ, a coactivator of TEAD transcription factors of Hippo signaling, was expressed under T H 17 cell-inducing conditions and was required for T H 17 differentiation and T H 17 cell-mediated inflammatory diseases. (nature.com)
  • Also, the pioneering transcription factors BATF and IRF4 are activated upon TCR/co-stimulation signals, which induce chromatin remodeling at Th17-associated genes, allowing chromatin to be accessible to other transcription factors. (rupress.org)
  • In this regard, we have identified the AP-1 family of transcription factors (TFs) as key regulators of Th17 cell identity. (jimmunol.org)
  • secrete
  • Unlike other small intestinal immune cells or circulating eosinophils, we found that small intestinal eosinophils have a unique ability to constitutively secrete high levels of IL-1 receptor antagonist (IL-1Ra), a natural inhibitor of IL-1β. (rupress.org)
  • significantly
  • Inhibition of the Akt signaling pathway significantly decreases Th17 cell migration. (sigmaaldrich.com)
  • Depletion of CD4(+) T cells led to a significantly decreased frequency and number of IL-17A(+) lymphocytes and was sufficient to prevent acute rejection and OB. (sigmaaldrich.com)