• This is a Rho-dependent terminator located within the first translated gene of the left operon and is regulated by P4 immunity. (unimi.it)
  • This relies on recombinant nucleic acid techniques to form new combinations of heritable genetic material followed by the incorporation of that material either indirectly through a vector system or directly through micro-injection, macro-injection or micro-encapsulation. (wikipedia.org)
  • One of the approaches based on next-generation sequencing (NGS) is targeted enrichment of genomic DNA (TEDNA-seq) sequencing, which allows the identification of a directly selected genomic region by using hybridization probes during DNA library preparation. (springer.com)
  • The discovery of a genomic region in the Toledo strain that had been deleted from the prototype laboratory strain AD169 [ 5 ], added a new set of previously unrecognized open reading frames (ORFs). (biomedcentral.com)
  • The first and possibly most important aspect considered is the molecular genetic characterization provided by the industry for gaining approval. (i-sis.org.uk)
  • A Pilot Project was set up by the Biotechnology Group in the Trans-Atlantic Economic Partnership Action Plan (TEP) to compare the molecular genetic characterization that industry has to submit on both sides of the Atlantic to gain regulatory approval for release to the environment. (i-sis.org.uk)
  • The key outcome of the Workshop will be a technical annex (Annex 3) of the molecular genetic characterization required on both sides. (i-sis.org.uk)
  • A draft Annex 3 circulated for comments after the workshop was actually drawn up as the result of an earlier meeting of regulatory officials from the United States and Canada aimed at comparing and harmonizing the molecular genetic characterization of the two countries. (i-sis.org.uk)
  • The molecular genetic characterization in the draft Annex 3 does not take account of the random nature of the genetic transformation process. (i-sis.org.uk)
  • The aim of the present study was to analyse the effect of PLCD4, PECR, FN1 and PNKD mutations on pig productive traits and tested the usefulness of targeted enrichment DNA sequencing method as tool for preselection of genetic markers. (springer.com)
  • Aortic dilatation, dissection, or sudden death was the index event leading to genetic diagnosis in 65% of families with TGFBR2 mutations, versus 32% with FBN1 mutations ( P =0.002). (ahajournals.org)
  • Several large cohort studies have attempted to define the genetic basis of type 1 VWD, and numerous mutations have been reported. (bloodjournal.org)
  • However, no information is available on the deletions or mutations in the BART and BHRF regions in clinical samples of EBV-associated lymphoma. (biomedcentral.com)
  • Therefore, there is no information about the deletions or mutations in the BART and BHRF regions in clinical samples of EBV-associated lymphoma. (biomedcentral.com)
  • Genetic sequencing compounds the problem because we can now look into the genetic makeup of an animal and see how closely-or not-it's related to a distant relative. (mi-centre.com)
  • The potential genetic markers for pig productive traits were identified by using targeted enrichment DNA sequencing of chromosome 15 region that is QTL-rich. (springer.com)
  • The number of subjects with this type of condition is expected to increase due to better prevention of complications and genetic diagnosis advances, thus, prevalence data are essential for future budget estimates. (springer.com)
  • The aim of this study is to estimate the overall and selective prevalence rates of inherited muscle diseases in a northern Spanish region and to describe their demographic and genetic features. (springer.com)
  • Prevalence of inherited muscle diseases in Navarre is high in comparison with the data reported for other geographical regions. (springer.com)
  • The aim of our study is to describe the demographic and genetic features of this group of neuromuscular diseases in Navarre for a 16-year period (2000-2015) and to estimate the prevalence according to IMD subtype, age group and geographical distribution. (springer.com)
  • While there are many genetic scientists who have pure intentions to make the worst diseases today a thing of the past, it is also believed that there are others who would take the research too far. (mi-centre.com)
  • In this region, the abundance of QTLs related to meat quality traits, in particular meat texture, is the highest in comparison to other pig chromosomes. (springer.com)
  • Therefore, the analysis of thermal dissociation curves targeting the conserved kinetoplast DNA minicircles region is able to provide a rapid and reliable method to identify the main etiologic agents of cutaneous and visceral leishmaniases in endemic regions of Brazil. (biomedcentral.com)
  • IMPORTANCE The availability of genetic tools and laboratory models determines the progress in understanding mechanisms of virus emergence and pathogenesis. (asm.org)
  • Frederick Sanger developed a method for sequencing DNA in 1977, greatly increasing the genetic information available to researchers. (wikipedia.org)
  • Type 1 VWD is the most common form of the disorder 1 but can be difficult to diagnose due to heterogeneity of its clinical and laboratory phenotypes, which reflect heterogeneity in the genetic basis and pathogenic mechanisms of this disease. (bloodjournal.org)
  • This underscores the necessity for the development of a reverse genetic system based on an epidemic ZIKV strain. (asm.org)
  • B95-8, an EBV reference strain, has a deletion in the BART region. (biomedcentral.com)
  • In this study, DNA fragments encoding miR-BARTs and miR-BHRF1 were amplified by PCR from DNA samples extracted from EBV-positive lymphoma patients and were directly sequenced to determine the mutation, deletion, and/or insertion status of the regions encoding miRNAs. (biomedcentral.com)
  • The tol-oprL region in Pseudomonas aeruginosa appears to be involved in pyocin uptake and required for cell viability. (asm.org)
  • For S. aureus , little is known about the genetic basis of hypermutability. (asm.org)
  • Genetic engineering has been applied in numerous fields including research, medicine, industrial biotechnology and agriculture. (wikipedia.org)
  • Patent protection effectively promotes genetic engineering research and helps to ensure its speedy development. (mi-centre.com)
  • Human directed genetic manipulation began with the domestication of plants and animals through artificial selection in about 12,000 BC. (wikipedia.org)
  • The first company to focus on genetic engineering, Genentech, was founded in 1976 and started the production of human proteins. (wikipedia.org)
  • For other bacteria with limited MGE repertoire and routes of DNA transfer, the means of genetic exchange are not so obvious. (biomedcentral.com)
  • One strategic requirement of plant synthetic biology is the adoption of common standardized technologies that facilitate the construction of increasingly complex multigene structures at the DNA level while enabling the exchange of genetic building blocks among plant bioengineers. (plantphysiol.org)
  • Specific concerns over genetic engineering include: Regulation of genetic engineering The regulation of genetic engineering concerns the approaches taken by governments to assess and manage the risks associated with the development and release of GMOs. (mi-centre.com)
  • The Peshawar team designed a system of 2 separate but coordinated gas-sensing genetic circuits. (idtdna.com)
  • New pregnancy-specific screening criteria (NSC) have been proposed to identify women who warrant GCK genetic testing. (diabetesjournals.org)
  • Plasmid DNA was isolated from at least 10 clones per region (Wizard Plus SV Minipreps, Promega), sequenced using vector-specific primers and the Big Dye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems), and analyzed on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems). (jneurosci.org)