M019 binds to FcγR bearing-monocytes both in vivo and in vitro and prevented immune cell infiltration into the CNS of treated mice. (bvsalud.org)
Differentiation3
Organogenesis is a complex developmental process requiring hierarchical cell and tissue differentiation, coordinated in time and space in response to changes in local and distant signaling cues. (biomedcentral.com)
In mouse, Atoh1 is necessary for haircell differentiation, but its role in specifying the equivalence group (proneural function) has been questioned and little is known about its upstream activators. (biologists.com)
As the equivalence group develops, a few cells upregulate Atoh1 expression and complete differentiation as hair cells. (biologists.com)
Inner ear7
This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. (biomedcentral.com)
This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. (biomedcentral.com)
Using exogenous stem cells to replace lost inner ear neurons is a potential strategy if stem cell-derived neurons can form central and peripheral connections, form synapses on hair cells and cochlear nucleus neurons, and re-establish functional and tonotopic circuits [ 6 ]. (biomedcentral.com)
We address these potential limitations by adopting the technique of blastocyst complementation (BC) to generate inner ear neurons from induced pluripotent stem cells (iPSCs). (biomedcentral.com)
Hair cells of the inner ear develop from an equivalence group marked by expression of the proneural gene Atoh1 . (biologists.com)
Sensory epithelia of the vertebrate inner ear consist of two cell types,hair cells and support cells. (biologists.com)
Peripheral1
Stimulation of NK cells with aggregated IgG1 resulted in the upregulation of HAVCR2 - the gene encoding T-cell immunoglobulin and mucin-containing domain (Tim)-3 - known to be involved in the induction of peripheral T cell tolerance. (bvsalud.org)
Tissues1
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Sensory2
Hearing depends on the mechano-sensory hair cells (HCs) and their innervating neurons, the spiral ganglion neurons (SGNs), which are responsible for transmitting auditory information from the HCs in the organ of Corti to the cochlear nucleus in the brainstem. (biomedcentral.com)
prospective sensory cells begin to express p27 kip1 and exit the cell cycle before expression of Atoh1 , and this process still occurs in Atoh1 mutants. (biologists.com)
Genes1
A model is presented in which zebrafish atoh1 genes operate in a complex network leading to hair cell development. (biologists.com)
Equivalent1
there was an equivalent frequency of Th17 cells in the spleens of M019 and vehicle treated EAE induced mice. (bvsalud.org)
Process1
differentiate this process and differ being played by your books. (cdseidel.de)
Addition1
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Types1
Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
Download1
technology and expression of cell in original anything modern download materials. (cdseidel.de)
Expression3
Given the heightened interest in manipulation of co-signaling cascades for cancer immunotherapy, we sought to determine how/whether tumors decorated with therapeutic monoclonal antibodies (mAbs) impact the expression of co-signaling molecules on human NK cells. (bvsalud.org)
The rest lose expression of Atoh1 and become support cells. (biologists.com)
However, p27 kip1 expression and cell cycle withdrawal could be regulated independently from equivalence group specification. (biologists.com)
Development2
BC is a technique in which deletion of a key gene for the development of a specific lineage creates a vacant niche (organogenesis-disabled phenotype) that can be complemented by the progeny of wild type pluripotent stem cells injected into embryos at the blastocyst stage of development. (biomedcentral.com)
Two zebrafish homologs, atoh1a and atoh1b , are together necessary for hair cell development. (biologists.com)
Human2
In this report, we use recombinant human Fc multimers as stable IC mimics to show that C1q engagement of ICs directly and transiently inhibits their interactions with Fc[Formula: see text]RIII (CD16) on human natural killer (NK) cells. (bvsalud.org)
BC is the only current method for making fully functional, three-dimensional organs from pluripotent cells and generating human organs in large mammalian hosts may be able to address the critical worldwide problem of organ shortages for transplantation [ 16 ]. (biomedcentral.com)
Treatment2
While early adoption of these agents for the treatment of advanced squamous cell carcinoma of the head and neck (SCCHN) has produced some astounding clinical successes, the majority of patients fail to respond to therapy. (bvsalud.org)
Surgical treatment of a gastric ulcer includes severing the vagus nerve (vagotomy) to reduce the amount of gastric acid secreted by the gastric cells. (rnpedia.com)
Role1
Although C1q is traditionally viewed as a soluble effector molecule, we demonstrate that C1q may also take on the role of an "immunologic rheostat," buffering Fc[Formula: see text]R-mediated activation of immune cells by circulating ICs. (bvsalud.org)
Research1
During residency, he undertook a two-year NIH-sponsored research fellowship investigating mechanisms of hair cell degeneration. (stanford.edu)
Regenerate hair cells1
Yamoah overviewed the biological mechanisms of hearing and discussed his stem cell research, which seeks to regenerate hair cells of the inner ear in order to restore hearing. (ca.gov)
Differentiation1
This process involves the migration, growth, and differentiation of ENDOTHELIAL CELLS, which line the inside wall of blood vessels. (nih.gov)
Tumor4
National Cancer Institute 1 Introduction 1 Clinical investigations 3 Origins of the cancerous state 4 Development of clinical cancer 5 Autonomy 5 Spread of cancer 6 Wound washings 6 Cancer cells in circulating blood 7 Spread of tumor in animal models. (nih.gov)
In vivo delivery of TK-NTR and administration of prodrugs led to the effective killing of both targeted cells and surrounding tumor cells via TK-NTR-mediated conversion of co-delivered prodrugs into active cytotoxic agents. (regenerativemedicine.net)
In vivo evaluation of the bystander effect in mouse models demonstrated that for effective therapy, at least 1% of tumor cells need to be delivered with TK-NTR-encoding MCs. (regenerativemedicine.net)
These results suggest that MC delivery via microvesicles can mediate gene transfer to an extent that enables effective prodrug conversion and tumor cell death such that it comprises a promising approach to cancer therapy. (regenerativemedicine.net)
Functional1
Acceptance of other cell sources for functional integration, and identification of molecules and agents that impact this. (nih.gov)
Stem6
The purpose of the workshop was to discuss and identify potential areas of the auditory and vestibular systems that might be good candidates for applications being used in other reparative medicine systems, one being the potential use of stem cell biology. (nih.gov)
The workshop panel was composed of a diverse group of experts from auditory and vestibular systems as well as tissue engineers using aspects of stem cell biology in other reparative systems. (nih.gov)
She informed the group this workshop was not the forum to discuss ethical or developmental differences regarding stem cells themselves, but rather to focus attention on identifying and assessing areas of auditory and vestibular research potentially primed for reparative applications. (nih.gov)
Identification of stem or progenitor cells that give rise to hair cells/supporting cells in embryos and adults (human). (nih.gov)
Determination of the origin of regenerated hair cells from reserve stem cells or through dedifferentiation of other cells. (nih.gov)
Dolye briefly summarized the current clinical options for treating hearing loss and discussed the promise of stem cell research in overcoming the limitations of hearing loss treatment. (ca.gov)
Function2
The NIDCD held a workshop to explore the feasibility of reparative restoration of auditory and vestibular function. (nih.gov)
Hearing loss is the leading birth defect in the United States with ~3 children in 1,000 born with partial to profound compromise of auditory function. (ca.gov)
Coding sequence1
lt;p>The National Eye Institute (NEI) has developed a tissue-specific gene therapy approach based upon adeno-associated virus (AAV) mediated delivery of the full coding sequence for human retinoschisin to retinal cells under the control of a retinoschisin promoter. (nih.gov)
Biological1
Identification and measurement of biological functions of supporting cells. (nih.gov)
Protein2
HN - 2024 BX - Aflatoxicosis BX - Aflatoxin Toxicity MH - AIRE Protein UI - D000096927 MN - D12.776.930.123 MS - A transcriptional regulator primarily expressed in medullary thymic epithelial cells. (nih.gov)
Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
Screen1
Establishment of organ culture systems of animal explants capable of regenerating hair cells to screen for agents or conditions that stimulate or repress hair cell development. (nih.gov)
Control1
Characterization of the extracellular matrix components that control the stability or expansion of hair cells. (nih.gov)
Delivery2
We previously demonstrated that microvesicles can functionally deliver plasmid DNA to cells and showed that plasmid size and sequence, in part, determine the delivery efficiency. (regenerativemedicine.net)
We demonstrated that MCs can be loaded into shed microvesicles with greater efficiency than their parental plasmid counterparts and that microvesicle-mediated MC delivery led to significantly higher and more prolonged transgene expression in recipient cells than microvesicles loaded with the parental plasmid. (regenerativemedicine.net)