Terminal differentiation granulocytic differentiation of murine hematopoietic cells. Medical search. Frequent questions

Mononuclear myeloid cells include terminally differentiated macrophages and dendritic cells (DC), as well as monocytes, which under inflammatory conditions differentiate in tissues to macrophages and DCs. (aacrjournals.org)
Interestingly, we did not find any differences in the percentage of proinflammatory or Retigabine dihydrochloride anti-inflammatory macrophages and monocytes between the cell treated and MI control groups. (cell-signaling-pathways.com)

It has been hypothesized that it acts as a survival factor in tumor cell lines, preventing therapeutic-induced apoptosis and rendering the tumor cells more resistant to current treatments. (wikipedia.org)
TGF-β, along with other TGF-β family ligands such as bone morphogenic protein (BMP) and activin are involved in regulating important cellular functions such as proliferation, differentiation, apoptosis, and matrix production. (wikipedia.org)
We found that, in the erythropoietin-induced, CD34-positive hematopoietic stem cell (HSC) differentiation system, knockdown of p19 INK4d delays terminal erythroid differentiation, inhibits erythroblast growth due to increased apoptosis, and leads to the generation of abnormally nucleated late-stage erythroblasts. (biomedcentral.com)
The paradox of proliferative bone marrow together with peripheral blood cytopenias in MDS may be explained by increased intramedullary myeloid precursor cell apoptosis [ 6 ]. (medilib.ir)

Cell cycle and differentiation In vitro experiments using both human and mouse cell lines have shown that EVI1 prevents the terminal differentiation of bone marrow progenitor cells to granulocytes and erythroid cells, however it favors the differentiation of hematopoietic cell to megakaryocytes. (wikipedia.org)
Global transcriptome analyses of human and murine terminal erythroid differentiation. (biomedcentral.com)

TGF-β signaling induces transcription of the cyclin-dependent kinase (CDK) inhibitors p15Ink4B or p21Cip1, which, as a consequence, act to halt the cell cycle and stop proliferation. (wikipedia.org)
Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
Cul4a targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis. (biomedcentral.com)
Polypyrimidine tract-binding protein induces p19(ink4d) expression and inhibits the proliferation of h1299 cells. (biomedcentral.com)
P19ink4d is a tumor suppressor and controls pituitary anterior lobe cell proliferation. (biomedcentral.com)
To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions. (lookformedical.com)

The chimeric gene of AML1-MDS1-EVI1 (AME) formed by the chromosomal translocation (3;21)(q26;q22) has also been shown in vitro to upregulate the cell cycle and block granulocytic differentiation of murine hematopoietic cells, as well as to delay the myeloid differentiation of bone marrow progenitors. (wikipedia.org)
The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)
Thus, during normal hematopoiesis, there exists a cellular hierarchy headed by a stable population of pluripotent stem cells that generate lineage-specific progenitors, which differentiate into the various types of mature blood cells [ 2 ]. (medilib.ir)

Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)

The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
Effective hematopoiesis is facilitated by interactions of hematopoietic growth factors, various receptors, and the bone marrow microenvironment. (medilib.ir)

Normally, cyclin interacts with cyclin-dependent kinase (CDK) to form a cyclin-CDK complex, which promotes cell cycle progression, whereas cyclin-dependent kinase inhibitor (CDKI) molecules inhibit the formation of cyclin-CDK complex, arresting cell cycle. (biomedcentral.com)
During the 1980s and early 1990s, work from the laboratories of Diana Lopez, Jim Talmadge, M. Rita Young, and Hans Schreiber demonstrated that various types of myeloid cells could inhibit immune function in cancer. (aacrjournals.org)

Δ324 found at low levels in human and mouse cells - an alternative splice variant encoding an 88kDa protein lacking zinc fingers 6 and 7 Δ105 variant is unique to mice, and results in a protein truncated by 105 amino acids at the acidic C-terminus. (wikipedia.org)

EVI1 is a nuclear transcription factor involved in many signaling pathways for both coexpression and coactivation of cell cycle genes. (wikipedia.org)
The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES , and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways. (lookformedical.com)

We used the Video Performance Evaluation Resource (ViPER) software (Doermann and Mihalcik, 2000) to manually draw bounding boxes around cells in each video frame and index the sequences of bounding boxes corresponding JQ1 in vitro to each individual cell to designate tracks. (peptidesolubility.com)
Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)

Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
A family of transcription factors that share an N-terminal HELIX-TURN-HELIX MOTIF and bind INTERFERON-inducible promoters to control GENE expression . (lookformedical.com)

EVI1 was first identified as a common retroviral integration site in AKXD murine myeloid tumors. (wikipedia.org)
The observations of the accumulation in spleens and tumors of large numbers of these cells with potent immune-suppressive activity were readily reproducible in most murine tumor models. (aacrjournals.org)

This process is epitomized by myeloid-derived suppressor cells (MDSC). (aacrjournals.org)

CLASSIFICATION OF HEMATOLOGIC MALIGNANCIES - The hematopoietic pluripotent stem cell is capable of both self-renewal and stepwise differentiation into either the lymphoid or myeloid lineage (which appears to be determined stochastically) [ 1 ]. (medilib.ir)

The neutrophil maturation and differentiation pathway termed myelopoiesis takes approximately 12 days. (medscape.com)

Clonal studies in the chronic myeloid disorders - Genetic and enzyme studies based upon X-chromosome inactivation patterns have revealed a multipotent progenitor cell origin for the neoplastic clone in both MDS [ 8 ] and MPN [ 9 ]. (medilib.ir)
The clonal process may develop at different progenitor cell levels in individual patients, and the clonal progenitor cell may or may not involve T and B lymphocytes [ 10 ]. (medilib.ir)

cIAPs and XIAP negatively regulate cytokine production, including TNF to disrupt myeloid lineage differentiation. (ashpublications.org)

These cells are closely related to neutrophils and monocytes. (aacrjournals.org)
Granulocytic myeloid cells include populations of terminally differentiated polymorphonuclear neutrophils, eosinophils, basophils, and mast cells. (aacrjournals.org)
Myeloid cells generated under these conditions, although similar to neutrophils and monocytes in morphology and phenotype, have different genomic and biochemical profiles and functional activity. (aacrjournals.org)
The field started changing in the late 1990s when the Gr1 + CD11b + cellular phenotype was suggested as defining the immune-suppressive myeloid cells in spleens of mice and when these cells were shown to be phenotypically similar but functionally distinct from monocytes and neutrophils ( 2, 3 ). (aacrjournals.org)

BCI) Flow cytometric analysis of the various immune cell populations in the heart (BCH) and spleen (I) of C57BL/6J mice four and seven days following MI and cardiomyocyte transplantation (MIC). (cell-signaling-pathways.com)
The various cell populations were identified based on the strategy presented in Figure 1. (cell-signaling-pathways.com)
TIME possesses distinct populations of myeloid cells and lymphocytes to influence the immune escape of cancer, the response to immunotherapy, and the survival of patients. (biomedcentral.com)
Among the components of TME, distinct populations of innate and adaptive immune cells consist of tumor immune microenvironment (TIME). (biomedcentral.com)

MONOCYTES and a variety of other immune cells. (lookformedical.com)
Conclusion: Our work shows that cardiomyocyte transplantation alters the immune response after myocardial infarction with the recruited monocytes playing a role in the beneficial effect of cell transplantation. (cell-signaling-pathways.com)

It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. (lookformedical.com)

Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
and the innate immune cells contain natural killer (NK) cells and dendritic cells (DCs) (Fig. 1 ). (biomedcentral.com)

In conclusion, our study revealed the cell cycle-independent roles of p19 INK4d in human terminal erythropoiesis via a novel PEBP1- p ERK-HSP70-GATA1 pathway. (biomedcentral.com)
Inhibition of notch signaling by gamma secretase inhibitor engages the rb pathway and elicits cell cycle exit in t-cell acute lymphoblastic leukemia cells. (biomedcentral.com)

A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)

P19ink4d controls hematopoietic stem cells in a cell-autonomous manner during genotoxic stress and through the microenvironment during aging. (biomedcentral.com)

Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
TIME primarily consists of myeloid cells, lymphocytes and some other innate immune cells. (biomedcentral.com)

Unexpectedly, knockdown of p19 INK4d did not affect cell cycle, and these functions caused by p19 INK4d knockdown were via decreasing levels of GATA-binding protein 1 (GATA1). (biomedcentral.com)
However, as shown in our study, p19 INK4d played important roles independent of cell cycle regulation, and the lack of cell cycle change was probably due to the compensatory up-regulation of p18 INK4c following p19 INK4d knockdown. (biomedcentral.com)
Our findings provide greater understanding of the role that CDKIs play in cell cycle regulation. (biomedcentral.com)

All of these involve erratic cellular development and differentiation in the bone marrow leading to dramatic alterations in the normal population of blood cells. (wikipedia.org)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)

Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)

Innate immunity resides in the skin, mucous membranes, polymorphonuclear (PMN) cells, complement system, and a select group of cells that possess cytotoxic capabilities. (medscape.com)

The main functional characteristic of these cells is their potent ability to suppress various types of immune responses. (aacrjournals.org)

Cell-to-cell interactions set off a cascade of events that may result in T- or B-cell activation and, ultimately, host defense. (medscape.com)

However, the specific nature and biological significance of these cells remained largely unclear. (aacrjournals.org)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)

Over the past two decades, a series of significant advances have been achieved in exploring the biogenesis, abundance, and function of lncRNAs across different species and cell types. (biomedcentral.com)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)

NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)

Briefly, for testing cell growth in soft agar, 103 cells dissociated from neurospheres were suspended in 3 ml Adv DME containing 5% FBS and 0. (plksignaling.com)
We assessed the efficacy and safety of HER3-DXd in patients with epidermal growth factor receptor (EGFR)-mutated non-small-cell lung cancer (NSCLC). (bvsalud.org)

Cancer is not a chaotic malignant cell mass, but a delicate "hostile" organ, where many other cells are recruited and domesticated to become "accomplices", thereby protecting themselves from recognition and attack by the immune system [ 1 ]. (biomedcentral.com)

Myeloproliferative neoplasms (MPN), unlike MDS, usually exhibit terminal myeloid cell expansion in the peripheral blood [ 7 ]. (medilib.ir)
One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. (lookformedical.com)

A monocyte chemoattractant protein that has activity towards a broad variety of immune cell types. (lookformedical.com)

Some of the most common ones are: EVI_1a, EVI_1b, EVI_1c, EVI_1d, and EVI_3L are all variants in the 5' untranslated region, and all except EVI_1a are specific to human cells. (wikipedia.org)
Rp9 variant is quite common in human and mouse cells, lacks 9 amino acids in the repression domain. (wikipedia.org)
As reported in our article recently published in Blood entitled "Unexpected roles for p19 INK4d in posttranscriptional regulation of GATA1 and modulation of human terminal erythropoiesis" [ 3 ], we demonstrated what roles p19 INK4d plays in human terminal erythropoiesis. (biomedcentral.com)
Unexpected role for p19ink4d in post-transcriptional regulation of gata1 and modulation of human terminal erythropoiesis. (biomedcentral.com)

Cell surface glycoproteins that bind to chemokines and thus mediate the migration of pro-inflammatory molecules. (lookformedical.com)
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. (lookformedical.com)

It is constitutively expressed in the skin and may play a role in T-CELL trafficking during cutaneous INFLAMMATION. (lookformedical.com)

Classical activation of these cells takes place as a response to strong signals that usually come in the form of pathogen-associated molecular patterns (PAMP) or danger-associated molecular patterns (DAMP) molecules. (aacrjournals.org)
Specific molecular sites or structures on or in cells with which interferons react or to which they bind in order to modify the function of the cells. (lookformedical.com)

This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)

ECM is a macromolecular substance secreted by cells into the extracellular space and constitutes a complex network that supports tissue structure and the physiological activities of cells, including collagen, elastin fibrils, proteinases, proteoglycans (PGs), glycoproteins and glycosaminoglycans (GAGs). (biomedcentral.com)

In the lymphoid based contribution to the immune response, we observed a significant reduction in the percentage of CD4+FoxP3+ T cells (Figure 2F), commonly referred to as Treg cells and CD4+CD8+ T cells (Figure 2H) in the heart with a coincidental increase in the percentage of CD4+CD8+ T cells in the spleen (Figure 2I) four days after MI in the cardiomyocyte treated group compared to the MI control. (cell-signaling-pathways.com)

There Rabbit polyclonal to AGR3 was a slight reduction in the percentage of CD4+ T helper cells (Figure 2G) in the heart seven days after MI in the cardiomyocyte treated group compared to the MI control. (cell-signaling-pathways.com)

TME consists of cell component, the extracellular matrix (ECM) and abundant soluble signaling molecules. (biomedcentral.com)

These cells have emerged as an important contributor to tumor progression. (aacrjournals.org)
Reports on the accumulation of immune-suppressive myeloid cells associated with tumor progression were published sporadically beginning in the early 1970s ( 1 ). (aacrjournals.org)

However, the roles of p19 INK4d in terminal erythropoiesis are still unknown. (biomedcentral.com)

EVI1 is highly expressed in the murine embryo, found in the urinary system, lungs, and heart, but is only minutely detectable in most adult tissues, indicating a likely role in tissue development. (wikipedia.org)
They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. (lookformedical.com)

Myeloid cells are a highly diverse population. (aacrjournals.org)

Anatomy64

Organisms12

Diseases10

Chemicals and Drugs86

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes56

Disciplines and Occupations1

Information Science3

Macrophages2

Apoptosis4

Erythroid2

Proliferation8

Progenitors3

Assays1

Receptors3

Inhibit2

Lacking zinc fingers1

Pathways5

Vitro2

Gene2

Tumors2

Myeloid-derived suppressor cells1

Self-renewal1

Myelopoiesis1

Progenitor2

Disrupt1

Neutrophils4

Populations4

Monocytes2

Tumor cell lines1

Dendritic2

Pathway2

Physiologic1

Microenvironment1

Lymphocytes3

Cycle3

Bone2

Stem1

Polymorphonuclear1

Functional1

Interactions1

Biological2

LncRNAs1

Phagocytosis1

Potent1

Growth2

Malignant1

Peripheral blood2

Chemoattractant1

Human4

Inflammatory2

Inflammation1

Molecular2

Clonal1

Glycoproteins1

Spleen1

Helper1

Soluble1

Progression2

Roles1

Tissue2

Highly1