Teratocarcinoma cell pathways teratocarcinoma. Medical search. Frequent questions

It is widely used in biomedical research, mainly for studying developmental biology, stem cell biology, cell differentiation, and drug screening. (cytion.com)
As P19 cells have differentiation ability, they can be useful in investigating complex biological processes such as tissue formation and early embryonic development. (cytion.com)
Thus, P19 cells can be useful in studying cell differentiation and developmental processes. (cytion.com)
Wnt ligands are secreted glycoproteins that function in intracellular signaling pathways to regulate a variety of developmental processes including cell growth, cell differentiation, cell polarity, and apoptosis. (biomedcentral.com)
Role of Otx2 in Embryonic Stem Cells (ESCs): Otx2 is an intrinsic determinant required to maintain the ESC metastable state by antagonizing ground state pluripotency and promoting commitment to differentiation. (cnr.it)
CFC1 inhibited Activin A-induced NB cell differentiation and Smad2 phosphorylation in NB cell lines, indicating its involvement in tumorigenesis related to EGF-CFC co-receptor family molecule pathways. (oncotarget.com)
Accumulating evidence has shown that cancer stem cells (CSCs) are a small subpopulation of tumor cells that exhibit the properties of self-renewal, differentiation into various tumor cell types, tumorigenicity, and chemo/irradiation resistance [ 1 ]. (oncotarget.com)
Hinokitiol (β-thujaplicin) is a potent metal chelator that induces differentiation and apoptosis in teratocarcinoma F9 cells and tyrosinase, most likely via metal chelation. (enzolifesciences.com)
discovered the differentiation of alveolar type II epithelial cells in ECM extract [10] . (danabiosci.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
These cells are the original precursors of various cells in a tumor, whereas other intratumoral cells are limited in terms of their potential for proliferation and pluripotent differentiation. (biomedcentral.com)
In addition, normal adult stem cells (ASCs) self-renew, proliferate infinitely, have a multidirectional differentiation potential, similar to that of tumor cells, and can transform more easily into tumor stem cells than non-stem cells [ 20 , 21 ]. (biomedcentral.com)
Sodium butyrate induces histone hyperacetylation and differentiation of murine embryonal carcinoma cells. (rupress.org)
These results might indicate that the mechanism by which sodium butyrate promotes differentiation of EC cells is different from the ways in which RA and HMBA act. (rupress.org)
Differentiation-defective PCC4(RA)-1 EC cells fail to respond to RA, presumably because they possess minimal amounts of active binding protein for RA (cRABP). (rupress.org)
Sodium butyrate treatment of these cells results in only a modest level of differentiation. (rupress.org)
The latter observations suggest that sodium butyrate has the ability to reactivate a silent cRABP gene in PCC4(RA)-1 cells and thereby lead to extensive differentiation via the retinoid pathway when RA is added. (rupress.org)
Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
Together, our data demonstrate the fundamental role of Brd4 in monitoring cell differentiation through its interaction with acetylated histone marks and disruption of Brd4 may cause aberrant differentiation. (biomedcentral.com)

Red wine polyphenolic compounds inhibit vascular endothelial growth factor expression in vascular smooth muscle cells by preventing the activation of the p38 mitogen-activated protein kinase pathway. (igbmc.fr)

Supporting differences in Wnt signaling, gain of function of Wnt-1 in C57mg mouse mammary epithelial cells leads to their morphological transformation while loss of function of Wnt-5a leads to the same transformation. (biomedcentral.com)
To test the hypothesis that a specific cell line can respond to distinct Wnts with different patterns of gene expression, we over-expressed Wnt-5a and Rfz-2 in C57mg mammary epithelial cells and compared this cell line to C57mg cells over-expressing Wnt-1. (biomedcentral.com)
the first being a class that transforms C57mg mouse mammary epithelial cells and also promotes duplication of the dorso-ventral axis when over-expressed in Xenopus embryos. (biomedcentral.com)

The high metabolic activity and insufficient perfusion of tumors leads to the acidification of the tumor microenvironment (TME) that may inhibit the antitumor T cell activity. (unav.edu)
Markers have been most helpful in the workup of patients with germ cell tumors. (medscape.com)
In addition to their histologic characteristics, germ cell tumors retain molecular characteristics of their primordial lineage. (medscape.com)
Other biological markers for germ cell tumors include lactate dehydrogenase isoenzymes and placental alkaline phosphatase, although these are less specific. (medscape.com)
AFP is a glycoprotein produced by fetal yolk sac elements and is produced by a wide range of cancers , including gastric , liver, and colon adenocarcinoma , as well as extracranial germ cell tumors. (medscape.com)
AFP is markedly elevated with endodermal sinus tumors and elevated to a lesser degree with embryonal cell carcinomas. (medscape.com)
Neither of these proteins has proven valuable in the diagnosis of pineal parenchymal cell tumors. (medscape.com)
Non-germ cell tumors can derive from pineal parenchymal cells, as well as from surrounding tissue. (medscape.com)
Calcium may be present in either pineal cell tumors or astrocytomas. (medscape.com)
Our results suggest that Foxp3 exerts protumoral intrinsic effects in breast cancer cells and that gene-therapy-mediated blockade of Foxp3 could constitute a therapeutic strategy to improve the response of these tumors to standard treatment. (bvsalud.org)
Consistently, WHN-88 also notably restrained the progress of xenografted Wnt-driven human tumors, including PA-1 teratocarcinoma with high autocrine Wnt signaling and Aspc-1 pancreatic carcinoma with Wnt-sensitizing RNF43 mutation. (harvard.edu)
The identification of CSCs was achieved by isolating highly tumorigenic tumor cells from primary tumors and tumor cell lines. (oncotarget.com)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (sigmaaldrich.com)
According to the hypothesis, only a small group of stem cell-like tumor cells can form new tumors. (biomedcentral.com)
As a result, these liver organ immune system cells, including B cells and their cytokines, mainly become innate immune effectors against tumors and infections simply by their T helper-1 immune response in the liver organ. (enmd-2076.com)
The following text points out several possibilities and chemical pathways in which "Intume" chlorine dioxide intervention can destroy tumors and prevent proliferation of cancer cells. (justia.com)
Cancer cells are rich in polyamines which is necessary for survival and growth of tumors. (justia.com)

Foxp3 inhibition with P60 enhanced chemosensitivity and reduced cell survival and migration in human and murine breast tumor cells. (bvsalud.org)
We also developed an adenoviral vector encoding P60 (Ad.P60) that efficiently transduced breast tumor cells, reduced cell viability and migration, and improved the cytotoxic response to cisplatin. (bvsalud.org)
Conditioned medium from transduced breast tumor cells contained lower levels of IL-10 and improved the activation of splenic lymphocytes. (bvsalud.org)

Saracatinib, the first Src inhibitor to show inhibition of the Src pathway in human tumor tissue, has anti-tumor. (srcpathway.com)
He has also gained salient experience in various experimental setups using hematopoietic stem cells, bone marrow, cord blood-derived and adipose tissue-derived MSCs, very small embryonic-like stem cells, leukemic cells, and germline-derived cells. (livedna.net)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Errors arising in stem and progenitor cells have a much greater influence on the tissue in which they are found than errors arising in postmitotic differentiated cells. (biomedcentral.com)
When cells in some area of the body duplicate without control, the excess of tissue develops called tumor or neoplasm. (solutionpharmacy.in)
To do so, cells release signaling molecules that can move around within a tissue and be detected by receptors on other cells. (elifesciences.org)
We tend to assume that the signaling molecules are evenly distributed across a tissue and affect all the receiving cells in the same way. (elifesciences.org)
Cells responding to the signal somehow can ignore this noise and establish sharp boundaries between different cell types so that neighboring cells have distinct roles in the tissue. (elifesciences.org)
Oddly enough, the serum MCP-1 Metoclopramide HCl level elevated and peaked at six h after c-lipo pretreatment quickly, suggesting which the MCP-1 made by c-lipo-phagocytized Compact disc68+ Kupffer cells Metoclopramide HCl may recruit Compact disc11b+ macrophages in the periphery and bone tissue marrow. (enmd-2076.com)
ix Capacity of cancer tissue to produce abundant PA likely contributes to cancer cell's higher growth rates.ix ,x High concentrations of PA enhance cancer cells to invade, and is associated with less apoptosis and increased expression of the genes affecting metastasis.ix ,xi The association of increased polyamine synthesis and cancer was first reported in the late 1960s. (justia.com)

Cell Biol. (enzolifesciences.com)
J Cell Biol (1984) 98 (2): 602-608. (rupress.org)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
Novel evidence that the mannan-binding lectin pathway of complement activation plays a pivotal role in triggering mobilization of hematopoietic stem/progenitor cells by activation of both the complement and coagulation cascades. (livedna.net)

Results identified elevated CR-1 expression in esophageal squamous cell carcinoma (ESCC) specimens which correlated to poor prognosis of the patients. (cusabio.com)
Few diagnostic and prognostic biomarkers are available for head-and-neck squamous cell carcinoma (HNSCC). (biomedcentral.com)
Analysis of TCGA pan-cancer data revealed significant overexpression of CASC9 across different other entities including bladder, liver, lung and stomach cancers and especially in squamous cell carcinoma (SCC) of the lung. (biomedcentral.com)

We found that pharmacological inhibition of the acid loader chloride/bicarbonate anion exchanger 2 (Ae2), with 4,4'-diisothiocyanatostilbene-2,2'-disulfonicacid (DIDS) enhancedCD4(+) andCD8(+) T cell function upon TCR activation in vitro, especially under low pH conditions. (unav.edu)
Notably, genetic Ae2 silencing in OVA-specificT cells improvedCD4(+)/CD8(+) T cell function in vitro as well as their antitumor activity in vivo. (unav.edu)
Similarly, genetic modification of OVA-specificT cells to overexpress Hvcn1, a selectiveH(+) outward current mediator that prevents cell acidification, significantly improved T cell function in vitro, even at low pH conditions. (unav.edu)
Here we show that suboptimal T cell receptor stimulation of CD8+ T cells upregulates FOXP3 in vitro. (unav.edu)
We have now pre viously shown that FE65 improved cell surface ranges of ApoER2 in vitro. (srcpathway.com)
On this review, we examined the results of FE65 on VLDLR trafficking and processing and found that FE65 increases VLDLR within the cell surface in vitro, much like the result of FE65 on ApoER2 trafficking. (srcpathway.com)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
Scientifically speaking, organoids are in vitro generated three-dimensional (3D), mini-cluster of cells that highly simulate the structure and function of the corresponding organ in vivo [1] . (danabiosci.com)
Dr. Abdelbaset-Ismail has developed remarkable technical skills in cell transplantation for various animal models, molecular characterization, propagating cells in vitro and in vivo. (livedna.net)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Pluripotent cells can be differentiated into many different cell types in vitro. (biomedcentral.com)

Choriocarcinomas secrete large amounts of bhCG, and lesser elevations can occur in patients with embryonal cell carcinomas. (medscape.com)
Wnt/β-catenin signaling pathway is a classical and crucial oncogenic pathway in many carcinomas, and Porcupine (PORCN) is an O-acyltransferase, which is indispensable and highly specific for catalyzing palmitoylation of Wnt ligands and facilitating their secretion and biofunction. (harvard.edu)
We speculate that precancerous stem cells (pCSCs) arise during the evolution of carcinomas based on long-term experimental findings, published literature, and the cancer stem cell (CSC) theory, wherein pCSCs exist in precancerous lesions and have characteristics of both CSCs and normal stem cells. (biomedcentral.com)

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. (wikipedia.org)
GPI-anchored cell membrane protein involved in Nodal signaling. (cusabio.com)

Besides, this improvement was also observed by the depletion or inhibition of T regulatory cells. (unav.edu)
High regulatory T-cell count is associated with poor prognosis and seems to mediate immune tolerance in hepatocellular carcinoma. (bvsalud.org)
Forkhead box P3-peptide inhibitor P60 has been shown to specifically inhibit regulatory T-cell function in murine models. (bvsalud.org)
CONCLUSION: In this study, we showed that vaccination with alpha-fetoprotein-expressing dendritic cells in combination with a specific inhibition of regulatory T-cells by using P60 leads to synergistic tumor inhibition and prolonged survival. (bvsalud.org)
This emphasizes the importance of regulatory T-cells inhibition for obtaining an effective antitumoral immune response in hepatocellular carcinoma. (bvsalud.org)
In 1958 the remarkable analogy revealed by genetic analysis of lysogeny and that of the induced biosynthesis of ß-galactosidase led François Jacob, with Jacques Monod , to study the mechanisms responsible for the transfer of genetic information as well as the regulatory pathways which, in the bacterial cell, adjust the activity and synthesis of macromolecules. (nobelprize.org)
These findings reveal a novel regulatory pathway in which NO functions as a negative regulator of metabolic abnormalities induced by diabetes. (californiaehealth.org)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)

Vertebrate Wnt pathways can be functionally separated into two classes, the canonical Wnt/beta-catenin pathway and the non-canonical Wnt/Ca 2+ pathway. (biomedcentral.com)

BhCG is a glycoprotein with a half-life of 15-20 hours and is usually produced by placental trophoblastic cells. (medscape.com)
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. (nih.gov)

P19 is a type of pluripotent embryonal carcinoma cells originally obtained from teratocarcinoma developed in a C3H/He mouse. (cytion.com)
Pluripotent embryonic carcinoma cell line P19 is a useful model for studying early embryonic development. (cytion.com)
Pluripotent stem cells (PSCs) were first isolated and established from mouse embryos in 1981 [7] [8] . (danabiosci.com)
Human-induced pluripotent stem cells (iPSCs) were successfully produced by reprogramming mouse and human fibroblasts in 2006, which has had a major impact on stem cell and organoid research [12] . (danabiosci.com)
Organoids can be generated from two types of stem cells: pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), and organ-restricted adult stem cells (AdSCs). (danabiosci.com)
Embryonic stem cells (ESCs) are self-renewing, pluripotent cells derived from the inner cell mass (ICM) of a blastocyst [ 1 , 2 ]. (biomedcentral.com)
Histone acetylation has been implicated to be important for the pluripotent cell state, as some HDAC inhibitors have been shown to improve reprogramming efficiency of induced pluripotent stem cells (iPSCs) or maintain a pluripotent stem cell state. (biomedcentral.com)

Bystin in human cancer cells: intracellular localization and function in ribosome biogenesis. (nih.gov)
Pituitary sex hormones enhance the pro metastatic potential of human lung cancer cells by downregulating the intracellular expression of heme oxygenase 1. (livedna.net)
We demonstrate that RA forms a noisy gradient during critical stages of hindbrain patterning and that cells use distinct intracellular binding proteins to attenuate noise in RA levels. (elifesciences.org)
The Compact disc11b+ Kupffer cells portrayed intracellular TNF and surface area Fas-ligand (FasL). (enmd-2076.com)

Cell-associated TDGF1 acts as a Nodal coreceptor in cis. (cusabio.com)
Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. (cusabio.com)

Many downstream target genes of the Wnt/beta-catenin pathway have been identified. (biomedcentral.com)

P19 is a murine embryonal carcinoma cell line. (cytion.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. (nih.gov)
However, random variations (noise) that affect how many of these molecules are produced, how they move through the space between cells and how they bind to receptors makes the reality much more complex. (elifesciences.org)

Researchers use P19 cells to elucidate cell signaling pathways and cellular and molecular mechanisms involved in these processes. (cytion.com)
Molecular mechanism controlling the expression of the HOX Complex gene family in human teratocarcinoma cells (N-tera2/D1). (cnr.it)

In vivo, DIDS administration delayed B16OVA tumor growth in immunocompetent mice as monotherapy or when combined with adoptive T cell transfer of OVA-specificT cells. (unav.edu)
Secreted FKN inhibits lung adenocarcinoma growth in vivo through a prominent contribution of systemic effector NK cells and increased tumor immune infiltration. (bvsalud.org)
In that same study, we observed that FE65 increased sApoER2 and ApoER2 CTF in COS7 cells, whilst knockdown of FE65 brought on decreased ApoER2 CTF in vivo. (srcpathway.com)
The improvement of cell culture conditions through mimicking the in vivo microenvironment was made in 1987. (danabiosci.com)

Targeting the Cripto-1/TAK-1/NF-kappaB/Survivin pathway may be an effective approach to combat apoptosis resistance in cancer. (cusabio.com)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)

They can be differentiated into other cell types when exposed to non-toxic drugs such as retinoic acid and dimethyl sulfoxide (DMSO) [1]. (cytion.com)
Retinoic acid induces the development of neurons, microglia, and astroglia, whereas DMSO initiates beating cardiomyocytes and smooth muscle cell development from P19 cells. (cytion.com)
Expression analysis of the human Hox Complex in teratocarcinoma cells (N-tera2/D1) upon treatment with retinoic acid and the definition of temporal colinearity concept. (cnr.it)
Two cells in the same position can have very different retinoic acid levels, and the levels in a particular cell can vary from one minute to the next. (elifesciences.org)
The experiments also show that proteins that interact with retinoic acid help to reduce noise within a cell. (elifesciences.org)
Cells from embryonal carcinoma (EC) lines 6050AJ and PCC4.aza 1R differentiate in response to treatment with sodium butyrate as well as retinoic acid (RA) or hexamethylenebisacetamide (HMBA). (rupress.org)

We then focused on two genes previously linked to various cancers, mesothelin and stromelysin-1, which are respectively up-regulated by Wnt-1 and Wnt-5a in C57mg cells. (biomedcentral.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)

Vertebrate Wnts signal through frizzled receptors that in some experimental contexts (e.g., without co-expressing LRP5/6) preferentially activate the Wnt/beta-catenin or Wnt/Ca 2+ pathways [ 5 ]. (biomedcentral.com)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
Notch1 and Notch 2, but not Notch4, were visualized in the nucleus of EB cells, and all these receptors were also observed as patent cytoplasmic foci. (karger.com)
Human rhabdomyosarcoma cells express functional pituitary and gonadal sex hormone receptors: Therapeutic implications. (livedna.net)
In this study, we investigated the expression of serotoninergic receptors (5-HTR), the signaling pathway, and biological activity of 5-HT on human dendritic cells (DC), showing that immature and mature DC expressed mRNA for different serotoninergic receptors. (aai.org)

Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells. (cusabio.com)
Sphere formation, cell proliferation, colony formation in soft agar, and xenograft tumor formation were analyzed. (oncotarget.com)
Effects of lncRNA overexpression or downregulation on cell proliferation, clonogenicity, migration and chemosensitivity were studied in HNSCC cell lines. (biomedcentral.com)

Sleeping Elegance is far more susceptible to more than expression inhibition than piggyBac and Tol2, the cargo capacity of Sleeping Attractiveness is constrained, and contrary to Tol2 and piggyBac that Inhibitors,Modulators,Libraries are lively in all mamma lian cell kinds tested, Sleeping Elegance display cell form dependent exercise. (p450inhibitors.com)
Stem cells are most frequently cultured in Matrigel and in the presence of suitable exogenous factors including chemical small molecule inhibitors/activators, cytokines, and medium additives, and can be coaxed into forming organoids of corresponding organs. (danabiosci.com)
Gas7 and Circzfp644 functions were determined by miRNA-mimics and inhibitors in mouse teratocarcinoma cells (F9 cells), and luciferase gene reporter assay was assessed in the F9 cells. (besjournal.com)

Frozen P19 cell vials should be stored at below -150°C temperature in a freezer or the vapour phase of liquid nitrogen to maintain the viability of cells for the longer term. (cytion.com)
CM-1 or CM-ACF media can be used to freeze P19 cells using a slow freezing method that protects cells from any shock and preserve their viability. (cytion.com)
Unexpectedly, up- or downregulation of CASC9 expression in HNSCC cell lines with low or high CASC9 expression, respectively, did not result in significant changes of cell viability, clonogenicity, migration or chemosensitivity. (biomedcentral.com)

BACKGROUND & AIMS: Vaccination with tumor-associated antigen-pulsed dendritic cells leads to specific T-cell response against hepatocellular carcinoma. (bvsalud.org)
Aim of this study was to investigate whether P60 can improve the immune response induced by vaccination with adenovirus-transduced dendritic cells expressing alpha-fetoprotein in subcutaneous and orthotopic murine models for hepatocellular carcinoma. (bvsalud.org)
Additionally, mice were vaccinated twice with dendritic cells expressing alpha-fetoprotein. (bvsalud.org)
RESULTS: In a preventive setting prior to tumor engraftment, vaccination with alpha-fetoprotein-expressing dendritic cells significantly decreased tumor growth in a subcutaneous model (p = .0256), but no further effects were achieved by addition of P60. (bvsalud.org)
However, P60 enhanced the antitumoral effect of a vaccination with alpha-fetoprotein-expressing dendritic cells in established subcutaneous and orthotopic hepatocellular carcinoma characterized by high Treg levels (p = .011). (bvsalud.org)
Expressed in mature dendritic cells. (affbiotech.com)

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
It was not until 1998 that American biologist James Thomson isolated and cultured human embryonic stem cells from human blastocysts for the first time [11] . (danabiosci.com)

Beta-catenin then accumulates in the nucleus where it interacts with its transcriptional co-activators, members of the Lymphoid Enhancer Factor/T Cell Factor (LEF/TCF) family of high mobility group DNA binding proteins to regulate gene expression [ 1 ]. (biomedcentral.com)

CR-1 high cells isolated from ESCC cells possess cancer stem-like cells (ECSLC) properties. (cusabio.com)
Cisplatin upregulated Foxp3 expression in HER2+ and triple-negative breast cancer (TNBC) cells. (bvsalud.org)
High-dimensional systemic immune profiling is performed in a cohort of non-small-cell lung cancer patients undergoing PD-L1/PD-1 blockade immunotherapy. (bvsalud.org)
Additionally, we disclosed that WHN-88 inhibited cancer cell stemness obviously. (harvard.edu)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
The Wnt signal transduction pathway is important in a wide variety of developmental processes as well as in the genesis of human cancer. (biomedcentral.com)
Targeted therapies specifically target cancer cells while sparing healthy cells, leading to fewer side effects and improved patient outcomes. (patentpc.com)
Immunotherapies harness the body's immune system to recognize and destroy cancer cells, offering a novel and effective treatment option. (patentpc.com)
The antibodies disclosed herein can bind certain cancer cell surface. (patentpc.com)
Cancer stem cells (CSCs) are considered to be critically involved in the recurrence and metastasis of NB and are isolated as NB spheres. (oncotarget.com)
Therefore, CSCs have become promising target cancer cells for preventing cancer relapse and improving the prognosis of tumor patients [ 3 ]. (oncotarget.com)
Later on, Dr. Abdelbaset-Ismail then has joined the Stem Cell Institute at James Graham Brown Cancer, University of Louisville, as a Visiting Scholar from September 2013 to March 2014, where he has been then employed as a Postdoctoral Associate beginning May 2014. (livedna.net)
He has also much experience in context of homing, mobilization, and engraftment of hematopoietic stem cells and cancer research. (livedna.net)
Therefore, investigations on testing and maintaining the integrity of genomic DNA within precancerous stem cells (pCSCs), which are within the framework of the cancer stem cell (CSC) hypothesis, are necessary. (biomedcentral.com)
All types of cancers are common, in that, the cancer cells are abnormal and multiply out of control. (solutionpharmacy.in)
This type of cancer originates from the epithelial layer of cells that form the lining of external parts of the body or the internal linings of organs within the body. (solutionpharmacy.in)
Long non-coding RNAs (lncRNAs) have moved into the focus of cancer research as good candidates for tumor biomarkers and as regulators of various neoplastic cell properties. (biomedcentral.com)

Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
laid the foundations of brain organoids by demonstrating the self-organization of human brain iPSCs into neural cells that formed polarized cortical tissues [13] . (danabiosci.com)
The growth of neoplastic cells exceeds and is not coordinated with that of the normal tissues around it. (solutionpharmacy.in)
Animal cells need to be able to communicate with each other so that they can work together in tissues and organs. (elifesciences.org)
Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)

Cell death & disease. (tmu.edu.tw)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The unique properties of human stem cells have aroused considerable optimism about their potential as new pathways for alleviating human suffering caused by disease and injury. (edu.au)

In 1970 he began to study cultured mammalian cells, particularly certain aspects of their genetic properties. (nobelprize.org)

revealed that lung organoids can be produced from the 3D coculturing of endothelial cells and adult bronchioalveolar stem cells [17] . (danabiosci.com)

The transfected cells had been subjected to a chromosomal transposition assay to deter mine their transposition action. (p450inhibitors.com)
The adoptive transfer and cytotoxic assay against principal cultured hepatocytes verified the function of Compact disc11b+ Kupffer cells in CCl4-induced hepatitis. (enmd-2076.com)

Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
Nevertheless, every one of these studies have been based on information sets obtained by retrieving chromosomal targeting sequences from a mixed population of transposon targeted cells or using a PCR based method. (p450inhibitors.com)

In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)

Stem cell research began to flourish. (danabiosci.com)
Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
How, then, should embryonic stem cell research be regulated in Australia? (edu.au)

Is P19 an adherent cell line? (cytion.com)
P19 embryonic carcinoma cell line is adherent. (cytion.com)
Adherent cells are washed with 1 X phosphate buffer saline and incubated with Accutase until the cells dissociate. (cytion.com)
Adherent and semi-confluent layer of P19 cells at 10× and 20× magnification. (cytion.com)
Methods: The gene expression profiling of adherent (control) and sphere-forming primary NB cells was conducted using a gene expression microarray. (oncotarget.com)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)

We studied the role of the expression of the transcription factor FOXP3 on CD8+ T cell function and anti-tumor immunity. (unav.edu)

The cell pallet is again resuspended, and cells are poured into the new flask for growth. (cytion.com)
Results: The overexpression of CFC1 increased sphere formation, cell growth, and colony formation. (oncotarget.com)
The liver provides a fertile soil in which metastases may become established, not only because of its rich, dual blood supply but also because of humoral factors that promote cell growth. (medscape.com)

2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

Dr. Abdelbaset-Ismail is one of the founding members of International Perinatal Stem Cells Society, and he has received the very prestigious 2015 Abstract Achievement Award from the American Society of Hematology. (livedna.net)

The immunohistochemical evaluation from the liver organ and the stream cytometry from the liver organ mononuclear cells demonstrated that clodronate liposome (c-lipo) treatment significantly reduced the spindle-shaped F4/80+ or Compact disc68+ cells, as the oval-shaped F4/80+ Compact disc11b+ cells elevated. (enmd-2076.com)
Furthermore, anti-TNF Ab pretreatment (which reduced the FasL appearance of Compact disc11b+ Kupffer cells), anti-FasL Ab mice or pretreatment attenuated the liver organ injury induced by CCl4. (enmd-2076.com)
It really is popular that liver organ HGFB NK cells and NKT cells potently generate IFN- in response to IL-12 and/or LPS [11]C[13]. (enmd-2076.com)
Oddly enough, liver organ B cells (mainly B-2 cells) generate IL-12 and IFN- however, not IgM, in response to LPS (vice versa for spleen B cells) [14]. (enmd-2076.com)
Furthermore, these IL-12-making liver organ B cells, as opposed to spleen B cells, phagocytose bacterias and eliminate them [15], [16]. (enmd-2076.com)

When cancerous, the bone marrow begins to produce excessive immature white blood cells that fail to perform their usual actions and the patient is often prone to infection. (solutionpharmacy.in)

These originate in the plasma cells of bone marrow. (solutionpharmacy.in)
These cancers affect the bone marrow which is the site for blood cell production. (solutionpharmacy.in)

Contrast-enhanced CT scans taken in the portal venous phase show a hypervascular metastatic deposit from a renal cell carcinoma. (medscape.com)
The patient had a previous right hepatectomy for an earlier renal cell solitary metastatic deposit. (medscape.com)
In 2010, renal organoids were produced from murine fetus-derived kidney stem cells [15] . (danabiosci.com)

CFC1, which functions in the development of embryos and decides the left-right axis, was strongly expressed in sphere-forming cells only and was related to the unfavorable prognosis of NB patients. (oncotarget.com)

The CD11b+ Kupffer cells producing TNF and FasL play a pivotal role in CCl4-induced acute hepatic injury thus. (enmd-2076.com)

In contrast, we and many others have shown that FE65 decreased sAPPa in CHO cells, suggesting that the effects in selleck chemicals distinct cell forms may be because of various interacting proteins. (srcpathway.com)
Different Wnts have distinct effects on gene expression in a single cell line. (biomedcentral.com)
It is important to emphasize that whether Wnts activate distinct responses and likely pathways is context-dependent, and thus in other contexts a given Wnt may activate another pathway. (biomedcentral.com)

Anatomy10

Organisms3

Diseases6

Chemicals and Drugs15

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes14

Information Science3

Differentiation31

Mitogen-activa1

Epithelial cells3

Tumors17

Tumor cells3

Tissue10

Biol2

Progenitor cells2

Squamous cell carc3

Vitro11

Carcinomas3

Protein2

Regulatory9

Functionally1

Trophoblastic2

Pluripotent7

Intracellular4

TDGF12

Target genes1

Embryonal carcinoma cell line1

Molecules3

Molecular2

Vivo4

Apoptosis1

Cellular2

Retinoic acid6

Genes2

Receptors5

Proliferation3

Inhibitors3

Viability3

Dendritic cells6

Human embryonic stem2

Regulate1

Cancer18

Tissues5

Disease3

Mammalian cells1

Endothelial cells1

Assay2

Sequences2

Somatic cells1

Stem Cell Res3

Adherent5

Biology1

Anti-tumor1

Growth3

Potent1

ABSTRACT1

Liver5

Immature1

Bone marrow2

Renal3

Embryos1

Pivotal role1

Distinct3