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EnzymesAntarcticaEnzymeSilicaSubstrateMiehei lipaseNovozymHydrolysisRugosa lipaseSynthesisBiocatalysisImmobilizationCalBEsterasesCatalystBiocatalystEsterification reactionAqueousOrganicThermophilicImmobilisationPolyBiomassUtilizationFattyCatalyzeEnantioselectiveYieldFreeRoom temperatureSelectivityActivityCarbonateMagneticEstersGlycerolMixtureCompoundsVariationsReaction timePowderBulkDEGRADATIONActiveRange
Enzymes9
  • Other enzymes and lipases from other microorganisms were also tested in the biocatalytic esterification of mono-and disaccharides. (researchgate.net)
  • The inhibitors are perfectly suited for quality control of hydrolytic enzymes like lipases and esterases. (pharma-industry-review.com)
  • This process is able to utilize either the crude or purified forms of these enzymes in either free form or immobilized on support, while providing compounds of formula III having optical purity of 99 percent and above. (justia.com)
  • Additionally, future perspectives of AI application in enzymes, including lipase engineering, are deduced to help the redesign of enzymes and the reform of new functional biocatalysts. (bvsalud.org)
  • For the free and immobilized system, the enzymes retained 32% and 92% of their initial activities, respectively, at the end of 48 days of storage. (gazi.edu.tr)
  • Time lapse video showing how straightforward it is to use immobilized enzymes in a rotating bed reactor. (spinchem.com)
  • For the investigation of the solvent impact on the enzymes, lipases from different sources (Pseudomonas fluorescences, Rhizopus javanicus, Rhizopus niveus, Candida rugose and Porcine pancreas) were used. (um.si)
  • The enzymes (lipases from Candida rugosa and porcine pancreas) were immobilized on silica aerogels by sol-gel procedure followed by supercritical drying with CO2. (um.si)
  • Such immobilized enzymes were used as biocatalysts for esterification in supercritical CO2 and near critical propane at 40 °C and 100 bar. (um.si)
Antarctica8
  • Linoleic acid (LA) is converted to per-carboxylic acid catalyzed by an immobilized lipase from Candida antarctica (Novozym 435). (hindawi.com)
  • Among the various lipases studied so far, Novozym 435, a commercial preparation of Candida Antarctica lipase B, has been shown to be the most effective. (hindawi.com)
  • This study aimed to develop and investigate the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) (STY-DVB-M) particles in a magnetically stabilized fluidized bed reactor (MSFBR) operated in continuous mode. (bvsalud.org)
  • In this paper, we describe a simple alternative enzymatic process, in which castor oil is directly converted to an estolide mixture by Candida antarctica lipase A (CALA) catalyzed transesterification. (mdpi.com)
  • Bulk polymerization, especially at a temperature between 70 and 75°C, and preferably using immobilized lipase from Candida antarctica (Novozym 435) gave polycarbonates with weight-average molecular weights of 40000. (elsevierpure.com)
  • A statistics based design of experiments (Central Composite Design, CCD) was used in the present work to develop an empirical model for describing, quantitatively, the methanolysis reaction of canola oil by the commercially immobilized Candida antarctica lipase, i.e. (sharif.edu)
  • These unsaturated free fatty acids were epoxidized with 30% H2O2 using immobilized Candida antarctica Lipase-B (CAL-B) as biocatalyst. (udl.cat)
  • We have recently reported on a novel immobilization method for Candida antarctica Lipase B on fumed silica to improve the enzymatic activity in hexane. (inrae.fr)
Enzyme8
  • TAG: reaction temperature of 55 °C, enzyme dosage of 9.5 wt. (upm.edu.my)
  • However, few studies have directly utilized AI to engineer lipase by affecting the structure of the enzyme, and a set of research gaps needs to be explored. (bvsalud.org)
  • lipase (ABL, MTCC no. 5125)is being recognized as an efficient enzyme for the resolution of drugs and their intermediates. (csircentral.net)
  • The reaction factors under study were the amount of enzyme (x1), temperature of operation (x2) and the molar ratio of methanol to canola oil (x3). (sharif.edu)
  • The positive sign for the coe cients of the amount of enzyme and the temperature of the reaction indicated that the yield of the methyl ester increased with increased levels of x1 from 3 to 5 and factor x2 from 25 to 35 C. (sharif.edu)
  • The methyl ester yield, 84.42%, as the highest value obtained through experiment that was in agreement with the predicted yield 89.15%, resulted with use of the optimized operative parameters when enzyme amount, reaction temperature and methanol to canola oil molar ratio were 5%, 38 C and 3, respectively. (sharif.edu)
  • Excellent enantioselectivity (E ≥ 134) was achieved by the four supported lipases (E = 68 for free enzyme). (chemfaces.com)
  • The effects of oil/ethanol molar ratio, temperature, added water content, and amount of enzyme were analyzed. (mysciencework.com)
Silica3
  • 19.Feng J, He F, Zhuo RX, Polymerization of trimethylene carbonate with high molecular weight catalyzed by immobilized lipase on silica microparticles, Macromolecules 2002, 35, 7175. (fm88roundup.com)
  • As a solvent in the synthesis of isopropyl acetate, via esterification of acetic acid with isopropyl alcohol catalyzed by lipase immobilized on silica. (sigmaaldrich.com)
  • The next utilization of lipases was the use of on silica arerogel self-immobilized lipase from Porcine pancreas as catalyst for esterification reaction in near-critical propane at 40°C and 100 bar. (um.si)
Substrate6
  • With impressive substrate selectivity and fast reaction rates, lipases are excellent catalysts offering both a sustainable and cost-efficient alternative in your manufacturing. (novozymes.com)
  • EUCODIS also offers a choice of more than 30 additional lipases suitable for your substrate. (pharma-industry-review.com)
  • Additional protocols based on optimized network models have been used to predict and optimize lipase production as well as properties, namely, catalytic activity, stability, and substrate specificity. (bvsalud.org)
  • The synergistic increase in activity and stability of the lipase-polymer assembly was achieved due to the structure of assembly, in which the role of cyclodextrin could enrich substrate affecting molecular diffusion. (bvsalud.org)
  • A substrate giving a yellow coloured product was used to follow the reaction progress of an ester hydrolysis by an immobilized lipase. (spinchem.com)
  • This substrate is commonly used to screen and characterize lipases. (spinchem.com)
Miehei lipase1
  • The ethanolysis of sunflower oil (SFO) with Lipozyme (immobilizéd 1,3-specific Mucor miehei lipase) in a medium solely composed of substrates was investigated. (mysciencework.com)
Novozym1
  • In this study, polyglycerol-2 stearic acid esters (PG2SAEs) were synthesized by the enzymatic esterification of polyglycerol-2 (PG2) and stearic acid (SA) using the immobilized lipase Novozym 435 as a biocatalyst in a solvent-free system. (unimi.it)
Hydrolysis2
Rugosa lipase3
  • Efficient Candida rugosa lipase immobilization on Maghnite clay and application for the production of (1R)-(−)-Menthyl acetate. (chemfaces.com)
  • This work focused on the identification of natural, economical, and efficient supports for immobilization of Candida rugosa lipase (CRL) to catalyze the resolution of (±)-menthol. (chemfaces.com)
  • As a catalyst the Candida rugosa lipase was used. (um.si)
Synthesis6
  • Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
  • Upon request, EUCODIS also offers synthesis of customized lipase suicide inhibitors. (pharma-industry-review.com)
  • Besthway Immobilized Lipase is developed by Besthway Technology with modern genetic engineering, microbe fermentation and advanced post-process technology, which can efficiently catalyze the ester synthesis and transesterification reactions in the biodiesel production process, and can also be used in other oil and grease processing industries, with the advantages of mild reaction conditions, environmental friendliness, high reusability, and good stability. (foodgradeenzyme.com)
  • The objective of this work was contributed in developing techniques to obtain immobilized biocatalysts with low cost and high catalytic activity to be applied in the biodiesel synthesis by ethanol route. (usp.br)
  • In addition, the lipase-polymer assembly proved to be an efficient catalyst for biodiesel synthesis, with a biodiesel conversion 1.4 times that of CalB at 60 °C. Therefore, this simple and low-cost lipase-polymer assembly provides new possibilities for the construction of high-efficiency industrial biocatalytic catalysts. (bvsalud.org)
  • Recently, reports have emerged detailing lipase-catalyzed synthesis of estolides from free hydroxy fatty acids. (mdpi.com)
Biocatalysis1
  • Biocatalysis with lipases deliver cost savings, greater sustainability, and better productivity. (novozymes.com)
Immobilization1
  • The modification process imparted switchable wettability to the surface while maintaining the thermal stability and biocompatibility of the CF. During the ethanol transformation, the rapid, reversible cell adsorption and detachment of Saccharomyces cerevisiae were performed through the modulation of wettability, displaying the enhancement of immobilized biomass and immobilization efficiency from 2.20 g/L and 59.43% to 2.81 g/L and 93.32%, respectively. (edu.au)
CalB1
  • At the same time, the stability of CalB-PNIPAM212/ß-CD and CalB-PNIPAM428/γ-CD are slightly better than that of CalB under high temperature, organic solution and extreme pH conditions. (bvsalud.org)
Esterases3
  • The suicide inhibitors are developed for active site titration experiments and may be used for the quantitative determination of active lipases and esterases in complex protein mixtures. (pharma-industry-review.com)
  • The phosphonate-based molecules are available with either chromogenic (p-nitrophenyl derivatives) or fluorogenic (4-methyl-umbelliferyl derivative) groups for sensitive detection of hydrolytic activity of lipases and esterases. (pharma-industry-review.com)
  • Especially suitable for use with this process are the various known lipases and esterases, and Pseudomonas lipase is preferred. (justia.com)
Catalyst2
  • This perspective focuses on showing some of the advances on the use of lipases engineering as immobilized catalyst for regioselective deprotection processes for potential industrial production glycoderivatives building blocks. (genchemistry.org)
  • This research focuses on the production of biodiesel from plant resources, and further investigates the influences of key process parameters, such as the molar ratio of methanol to oil, catalyst concentration, reaction temperature, reaction period and stirring speed on the biodiesel yield. (scirp.org)
Biocatalyst2
  • furthermore, biocatalyst recycling was studied by assessing the residual activity of the lipase after each reaction cycle, up to 20 times. (unimi.it)
  • These results suggest that CRL immobilized on Maghnite-H has good potential as biocatalyst for the production of (1R)-(-)-Menthyl acetate . (chemfaces.com)
Esterification reaction1
  • Four variables from a lipase-catalyzed esterification reaction were optimized using a central composite rotatable design. (upm.edu.my)
Aqueous1
  • Stability and activity of these lipases in aqueous medium in supercritical $CO_2$ and liquid propane at 100 bar and 40°C were studied. (um.si)
Organic2
Thermophilic1
  • This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies. (beilstein-journals.org)
Immobilisation1
  • In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). (researchgate.net)
Poly2
  • 20.Zhang XZ, Zhuo RX, Dynamic properties of temperature-sensitive poly(N- isopropylacrylamide) gel cross-linked through siloxane linkage, Langmuir 2001, 17, 12. (fm88roundup.com)
  • Matsumura, S, Harai, S & Toshima, K 2000, ' Lipase-catalyzed polymerization of diethyl carbonate and diol to aliphatic poly(alkylene carbonate) ', Macromolecular Chemistry and Physics , vol. 201, no. 14, pp. 1632-1639. (elsevierpure.com)
Biomass1
  • This review provides a new horizon for developing effective and innovative AI tools for lipase production and engineering and facilitating lipase applications in the food industry and biomass conversion. (bvsalud.org)
Utilization2
  • It is based on the utilization of filamentous fungi whole cells with high lipase activity (glycerol ester hydrolase - E.C.3.1.1.3) immobilized in suitable support. (usp.br)
  • Based on the accumulation of the biofilm, the fabrication and utilization of PNIPAM-CF improved the efficiency of continuous immobilized fermentation, making the ethanol production reach 26.34 g/L in the sixth batch of fermentation. (edu.au)
Fatty3
  • In the first case, the fatty acid conversions were about 20 % greater when increasing temperature from 45 C to 55 C. (researchgate.net)
  • With a very high molar proportion of fatty acid to saccharide when compared to the other studies, the authors suggested that increasing the proportion of fatty acid could negatively affect the lipase conformation due to an acidification of the reaction medium. (researchgate.net)
  • In the lipase-mediated epoxidation of unsaturation fatty acid, the acid itself reacts with hydrogen peroxide to form the peracid, which then epoxidized the double bond. (hindawi.com)
Catalyze1
  • A milder and more selective epoxidation process has been suggested [ 4 ], wherein a lipase is used to catalyze the peracid formation. (hindawi.com)
Enantioselective1
  • Lipases are used in many industrial applications where the chemical transformations include enantioselective resolution of esters and amides. (novozymes.com)
Yield2
  • Different neural networks coupled with various algorithms are usually applied to predict the maximum yield of lipase by optimizing the external cultivations for lipase production, while one part is used to predict the molecule variations affecting the properties of lipase. (bvsalud.org)
  • Diethyl carbonate (DEC) and diols, such as 1,3-propanediol and 1,4-butanediol, were polymerized by a lipase at a temperature between 40 and 80°C to yield the corresponding aliphatic polycarbonates in a successive two-step polymerization. (elsevierpure.com)
Free7
  • Free and immobilised lipases were tested for their esterification activities. (researchgate.net)
  • The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
  • Recently, lipase-mediated epoxidation in a solvent-free medium has also been reported [ 6 ]. (hindawi.com)
  • K-m values of free and immobilized lipases were found to be 0.290, 0.422 mM while V-max values were calculated as 0.089, 0.080 mM.min(-1), respectively. (gazi.edu.tr)
  • Immobilized CRL on Maghnite-H exhibited good thermostability over a wide temperature range (30-90 °C) compared to the free one. (chemfaces.com)
  • It was found out that the initial reaction rates in propane rose two to three times in comparison with the same reaction, catalyzed by free lipase. (um.si)
Room temperature1
Selectivity1
Activity2
  • We described a broad analysis of animal and human S. saprophyticus isolates regarding virulence genes and phenotypic properties such as lipase activity, hydrophobicity, and adherence. (biomedcentral.com)
  • MSCRAMMS for adherence like sdrI and to modulate their existing properties e.g. increasing the lipase activity or reducing hydrophobicity. (biomedcentral.com)
Carbonate1
  • This was due to the partial degradation of both DEC and the carbonate moieties of the polymer chain by the lipase. (elsevierpure.com)
Magnetic1
  • By measuring the temperature variations during the application of the magnetic field, the total magnetic time constant including both Brownian and Neel relaxation times can be calculated. (sharif.edu)
Esters2
  • Differential scanning calorimetry (DSC) analysis of these esters revealed potential latent heats ranging from 136.83 kJ kg−1 to 234.22 kJ kg−1 and melting temperatures from 52.45 ◦C to 76.88 ◦C. Finally, the compounds with enthalpies above 200 kJ kg−1 were subjected to 100 and 1000 thermal cycles. (udl.cat)
  • en)Disclosed are novel (meth)acrylic esters of unsaturated amino alcohols, a process for their preparation and their use for preparing crosslinked swellable hydrogel-forming polymers and crosslinked swellable hydrogel-forming polymers. (vtex.vn)
Glycerol1
  • Further, utilizing different lipase specificities, addition of Thermomyces lanuginosus lipase (TLL), allowed the CALA product mixture to be cleaned up by hydrolyzing attached glycerol. (mdpi.com)
Mixture1
  • The reaction mixture is analyzed by NMR to determine the estolide number (EN) and MALDI MS to identify individual components, in addition to titration to determine the acid value (AV). Estolide trimers and tetramers (EN 2-3) were formed over 24 h in a system with 2:1 ( v/v ) castor oil-water. (mdpi.com)
Compounds1
  • However, the high susceptibility to environmental conditions such as high temperature, light, and oxygen leads to the degradation of these compounds. (afpm.org.my)
Variations1
Reaction time1
  • In this work, the effects of solvent, reaction time, and temperature, on the conversion as well as on enantioselectivity were investigated. (chemfaces.com)
Powder1
  • The precursors and catalysts were characterized by powder X-ray diffraction (XRD), temperature programmed reduction (TPR) and transmission electron microscopy (TEM). (ijche.com)
Bulk1
DEGRADATION1
  • The glass transition temperature was 85.68 °C, and the onset of thermal degradation occurred at 406.66 °C. Syntheses were performed at 50 °C using a space time of 12 h and a bed porosity of 0.892. (bvsalud.org)
Active1
  • Large amounts of the active phase can be immobilized due to the large specific surface area. (encyclopedia.pub)
Range2