Temperature cells cultured. Medical search. Frequent questions

At room temperature, PNHD nanogels are in their dispersion states, allowing them to be thoroughly mixed with inoculated cells. (rsc.org)
We incubated the treated surfaces at room temperature (21°C-22°C) for different time points as indicated and then immersed them in viral transport medium for 30 min to recover the residual infectious virus. (cdc.gov)
Bring all reagents and samples to room temperature before use. (rndsystems.com)
collections at room temperature. (bvsalud.org)
The dehydrated cyst-containing NNA was cut in rectangles and stored in airtight tubes at room temperature for up to 3 years. (bvsalud.org)
however, two strains showed reduced viability, at 66.7% and 62.5%, after 2 years of room temperature storage. (bvsalud.org)
The generic dynamic perturbation factor is less than 2 at room temperature and exhibits a maximum near 260 K. The dynamic perturbation is induced by H-bond constraints that interfere with the cooperative mechanism that facilitates rotation in bulk water. (lu.se)
Culture is performed on Thayer-Martin plates that must be stored refrigerated but warmed to room temperature before obtaining a sample. (medscape.com)
Stains are stored at room temperature. (who.int)

Interactions between light and various cells in cultures, such as bacteria or mammalian cells, are widely applied for optical sensors and optofluidic systems. (mdpi.com)
Coal: response of cultured mammalian cells corresponds to the prevalence of coal workers pneumoconiosis. (cdc.gov)

They need to be kept at body temperature (37 °C) in an incubator. (wikipedia.org)
We dedicated the Cell Culture Screening Solution exactly for these workflow steps - as it supports reproducible assay conditions regardless of the vessel location inside the incubator. (eppendorf.com)
CO 2 Incubator simplifies benchtop cell culturing. (thomasnet.com)
With its 1.4 cu ft (40 L) capacity, the Midi 40 incubator adds a new dimension to the Thermo Scientific portfolio of leading CO2 incubation solutions and delivers the reliable culturing performance of a full-sized incubator in a convenient, space-saving footprint. (thomasnet.com)
The easily stackable Thermo Scientific Midi 40 CO2 incubator is ideal for cell culturing scientists who need smaller, discrete workspaces or who require smaller sample throughput. (thomasnet.com)
The PHCbi large capacity CytoGrow Reach-In CO 2 incubator has the capacity and flexibility to grow with your culturing needs while providing a precise and repeatable temperature, humidity, and CO 2 environment. (pacificscience.com)
The MCO-80ICL-PA space-saving CO 2 incubator is ideal for culturing large volumes of biological samples, performing microbiological studies, and working with large volume cell apparatus. (pacificscience.com)
Enhance the production yield of your cell culture experiments with PHCbi's large capacity CO 2 incubator. (pacificscience.com)
Part of the Cell-IQ CO 2 series, the MCO-230AICUVLG-PA is the ideal high throughput cell culture incubator for culturing or protocols that may require the use of an experimental apparatus. (pacificscience.com)
The PHCbi brand Cell-IQ MCO-50AICL-PA incubator offers precise and accurate control of CO 2 , humidity, and temperature for optimal culturing conditions. (pacificscience.com)
The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)

Regarding next generation of adherent cell culture platforms such as the Corning Ascent FBR system or microcarriers paired with a bioreactor, there is additional built-in process control. (corning.com)
Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
Bioreactor flasks have a large surface area and volume, allowing for increased cell density and greater antibody production. (coleparmer.com)
Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
CellAdhere™ Dilution Buffer and non-tissue culture-treated cultureware (e.g. (stemcell.com)
Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
Show answer Why is the Leica DMi1 particularly suited to cell and tissue culture? (leica-microsystems.com)
For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
Stericup Quick Release-GP Sterile Vacuum Filtration System, Stericup Quick Release-GP sterile vacuum filtration system combines a 500 mL Steritop bottle top filter unit, which uses a 0.22 µm pore size fast flow PES Express PLUS membrane, with a 500 mL receiver flask for sterilizing tissue culture media, protein & DNA. (sigmaaldrich.com)
Coal particles were leached at physiologic temperature and pH with water, serum, or Eagle's tissue culture medium to simulate the in-vivo process. (cdc.gov)
2. Virkon and tissue culture hood with UV-light. (lu.se)
3. 15-cm tissue culture-treated plastic dishes. (lu.se)

Overexpression of p62 is likely related to tumorigenesis and has been observed in many types of tumors ( 13 , 21 - 23 ), such as chemotherapy-resistant epithelial cell carcinoma ( 24 ). (spandidos-publications.com)
We compiled data from ferrets inoculated with an extensive panel of over 50 human and zoonotic IAV (inclusive of swine-origin and high- and low-pathogenicity avian influenza viruses associated with human infection) under a consistent protocol, with all viruses concurrently tested in a human bronchial epithelial cell line (Calu-3). (cdc.gov)
We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models. (cdc.gov)

Screening assays involve the exposure of PBMCs to new potential antibodies, e.g. to test for toxicity with cell-based assays or ELISA. (eppendorf.com)
4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)

The increase in viable cells with culture time suggests that the in situ formed hydrogels support cell proliferation. (rsc.org)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
We hypothesized that, in CBD, failure of IL-10 to modulate the beryllium-specific, cell-mediated immune response would result in persistent, maximal cytokine production and T lymphocyte proliferation, thus contributing to the development of granulomatous lung disease. (cdc.gov)
bronchoalveolar lavage cells from control and CBD subjects to evaluate the beryllium salt-specific production of endogenous IL-10 and the effects of exogenous human rIL-10 (rhIL-10) on HLA expression, on the production of IL-2, IFN- , and TNF- , and on T lymphocyte proliferation. (cdc.gov)
We of both mitogen- and Ag-induced accessory cell-dependent T lym- phocyte proliferation. (cdc.gov)

Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)

Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)
The cytotoxic effects of the leachings on cultured fibroblasts parallels the epidemiology of human pneumoconiosis in the two mines. (cdc.gov)

Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
Then the in vivo sensitivity of these transfected C6-TK and C6-sr39TK cells to GCV was determined by 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric assay and Hoechst-propidium iodide (PI) staining. (hindawi.com)
The vectors are also used for in vivo modifications of cells, e.g. for reprogramming. (lu.se)

Researchers mapped ear canal shape to body temperature to predict when ancestors of mammals first became endothermic. (the-scientist.com)
The perception of nonpainful, cool temperatures is reported to occur when the skin is cooled as little as 1°C from normal body temperature [ 4 ]. (nih.gov)
SDTT can also arise because of physical differences that affect the relative body temperature dynamics of large and small organisms (physical SDTT). (bvsalud.org)
Blood also helps control body temperature, fluids, and the balance of chemicals. (medlineplus.gov)

The PHCbi Cell-IQ™ CO2 incubators provide optimal cultivation conditions with precise CO2 concentration, temperature, and humidity while remaining easy to clean and operate. (pacificscience.com)

The cells are then thawed and seeded, usually into a small vessel like a T-flask. (corning.com)
The right culture vessel is crucial here, and different cell types may require different surfaces, such as plastic, glass, or coated surfaces. (coleparmer.com)
Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

On the subject of integration and "complete solutions," GE Healthcare's ReadyToProcess™ product line covers all unit operations from media preparation and cell cultivation to protein purification. (genengnews.com)
Monitoring and maintaining robust cell health and protein quality are critical to scale from the lab to commercialization. (beckman.com)
Producing highly productive cell lines and optimized cell culture conditions requires an understanding of the contributions of many key variables: cell culture media, cell growth rates, cell viability, protein expression levels, and other quality attributes including size, aggregation status, glycosylation and stability. (beckman.com)
p62 is a multifunctional protein that mediates cell signaling pathways, autophagy and tumorigenesis, and participates in important regulation processes at the intersection between autophagy and cancer. (spandidos-publications.com)
Cells overexpressing p62 showed a higher cell death rate than cells not expressing this protein. (spandidos-publications.com)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
The substrate temperature should be maintained between 70 and 75° F and kept saturated until all seeds have germinated. (gpnmag.com)
Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
The HaCaT human keratinocytes (provided by RWTH, Aachen, Germany) were genetically modified at the Institute of Anatomy and Cell Biology of the RWTH, Aachen (laboratory of CJ Wruck). (europa.eu)
Just because any area of science features thermal-induced processes (thermodynamics, chemical synthesis, fluid dynamics, phase transitions, cell biology, etc), the possible systems of interest that our technique can address is countless, with only one limitation : the imagination. (fresnel.fr)
So far, thanks to this microscopy technique, we have been able to address problems in physics, chemistry and cell biology. (fresnel.fr)
We study problems in thermal biology at the level of single cells generated by heating of gold nanoparticles, such as the heat-shock response of single living cells [30]. (fresnel.fr)
It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)

Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. (sigmaaldrich.com)
The foundation of the collection was laid in Hamburg in 1974 with the establishment of clonal algae cultures from environmental isolates. (uni-due.de)
The strains are clonal or single cell isolates, if not otherwise stated. (uni-due.de)
Overview of Myeloproliferative Neoplasms Myeloproliferative neoplasms are clonal proliferations of bone marrow stem cells, which can manifest as an increased number of platelets, red blood cells (RBCs), or white blood cells (WBCs). (msdmanuals.com)

The changes can be accounted for by changes of balance between the elasticity of the cell walls and the osmotic pressure across the walls. (microbiologyresearch.org)

You may already know that to produce mAbs, large quantities of hybridoma cells are needed. (coleparmer.com)

Moreover, even when high-purity single-cell sorting can be achieved, viability after sorting is often low to moderate, especially for cell types that are particularly sensitive to hydrodynamic stress or low-density culture conditions (e.g., primary cells or pluripotent stem cell lines). (nature.com)
Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)
We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
However, culturing for Neisseria is problematic because it is difficult to maintain the bacteria's viability during transport and storage in various settings. (medscape.com)

However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
For the in vitro assay, HepG2 cells were treated with FFAs (oleate/palmitate = 2:1) with or without treatment with R. oldhamii leaf ethyl acetate (EtOAc) fraction to observe lipid accumulation using Nile red and oil red O stains. (medsci.org)
However, in the mid- to late 1990s, a number of laboratories interested in cold transduction began to use primary cultures of either dorsal root (DRG) or trigeminal (TG) ganglia neurons as in vitro models of sensory afferents. (nih.gov)
The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
Viral titers in ferret nasal wash specimens and nasal turbinate tissue correlated positively with peak titer in Calu-3 cells, whereas additional phenotypic and molecular determinants of influenza virus virulence and transmissibility in ferrets varied in their association with in vitro viral titer measurements. (cdc.gov)

check cells for contamination (yeast, bacteria etc. (leica-microsystems.com)
Green, bacteria-free algae with lunate cells. (carolina.com)

Selection of edited cell clones currently relies on limiting dilution or fluorescence-activated cell sorting (FACS)-based single-cell sorting to isolate single cells. (nature.com)
When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)

Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

One big challenge with suspension cells is aggregation, which means your cells clump together and make it difficult to maintain single-cell suspensions. (coleparmer.com)
For suspension cells, use conical tubes or specialized culture vessels to keep cells in suspension and prevent aggregation. (coleparmer.com)
There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
Forced-floating: a cell suspension is loaded into the wells of a low adhesion polymer-coated microplate. (integra-biosciences.com)
Hanging drop: a cell suspension is loaded into the wells of a hanging drop plate. (integra-biosciences.com)
trophozoites (106 cells/mL) in PYG media or 150 µl of amoeba suspension (106 cells or cysts/mL) from monoxenic plate culture was spread onto the surface of non-nutritive agar (NNA, 2-3-mm thick) without or with a layer of heat-inactivated Escherichia coli, respectively. (bvsalud.org)

Disposables play an obvious role in scaleup, i.e., that form factors for smaller single-use cell culture vessels (e.g. (genengnews.com)
For example, if people want something 2D, they can try a stacked option such as Corning CellSTACK ® culture chambers or the more compact Corning HYPERStack ® cell culture vessels . (corning.com)
Those cells would then expand and be harvested and passaged to larger vessels. (corning.com)
To prevent this, it's important to create single-cell suspensions by gently pipetting or using specialized culture vessels. (coleparmer.com)

PDT uses a modality-based photosensitizer, which selectively affects cancer cells, using excitation and light-absorption in the presence of oxygen to produce a high concentration of reactive oxygen species (ROS), such as singlet oxygen and other free radicals ( 7 - 9 ). (spandidos-publications.com)
HA obtained by PEG concentration of BHK-21 cell culture fluid(15). (cdc.gov)
Coal leachings from a mine where the incidence of coal workers pneumoconiosis was very high were added to the culture medium of mouse L-cells and were found to inhibit the cellular growth in direct proportion to the concentration of coal leachates. (cdc.gov)
Nickel (7440020) was leached into the culture medium and found to be in a higher concentration in the more toxic coal sample. (cdc.gov)
Standard description of a patient's hemoglobin composition places the hemoglobin of greatest concentration first (eg, AS in sickle cell trait). (msdmanuals.com)

Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)
In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)

One hundred percent of the cyst samples produced axenically and maintained in dry NNA allowed the re-establishment of axenic cultures through direct incubation in PYG, with excystment occurring within 24 or 48 h. (bvsalud.org)
After incubation cultures are stored at 4°C in a refrigerator. (who.int)

Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
For more sophisticated experiments with GFP expressing cells or tissues, such as time-lapse or z-stacking with subsequent deconvolution, we recommend the Leica DMi8 modular inverted microscope system. (leica-microsystems.com)
Stocks of the LuSens cell line are stored in liquid nitrogen in the cell bank of ICCR-Roßdorf GmbH (aliquots of cells in freezing medium at 1.5-5 × 106 cells/cryovial) allowing the repeated use of the same cell culture batch in experiments. (europa.eu)
Therefore, the parameters of the experiments remain similar, because of the reproducible characteristics of the cells. (europa.eu)
We published a critique of the state of the art concerning temperature mapping in living cells, questioning the validity of recent experiments [20,22]. (fresnel.fr)
This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
We focused on kidney-RNAs reviewing literature for DKD-linked miRNAs from kidney tissue, cell culture and uEV/urine experiments. (lu.se)

The temperature gradation on a metallic culture surface facilitates immediate and accurate control of cellular temperature stimuli, as well as simultaneous evaluation of cellular responses to various temperature stimuli. (eurekalert.org)
At the level of the primary afferent nerve, the site at which thermal stimuli are converted into neuronal activity, temperature-sensitive members of the TRP channel family are found. (nih.gov)

Some corals can adjust to a range of temperatures, but this ability may be limited in a consistently warmer environment. (the-scientist.com)
Remarkably, the range of temperatures that these channels respond to covers the entire perceived temperature spectrum, from warm to painfully hot, from pleasingly cool to excruciatingly cold [ 1 ]. (nih.gov)

Gram stains, cultures, and nucleic acid amplification tests (NAATs) of genital, rectal, conjunctival, pharyngeal secretions and/or other extragenital sites, as applicable, should also be obtained when DGI is suspected. (medscape.com)
Gram stains can be made from blood culture and solid culture. (who.int)

However, performing the more sensitive PCR assay-based tests to raise the likelihood of detecting an infection and then following up with culture to produce admissible evidence is appropriate. (medscape.com)

The spherical shape of the released cells indicates their 3D growth in the absence of a physical scaffold. (rsc.org)
These cells support the growth and maintenance of other cells in culture. (coleparmer.com)
Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)
They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
Device's rapid recovery rates promote optimal cell growth while direct heating provides temperature control with uniformity of 0.1Ã‚Â°C. (thomasnet.com)
Rapid recovery rates promote optimal cell growth and highly efficient direct heating design provides extremely accurate temperature control with a uniformity of 0.1Â°C. (thomasnet.com)
Ham′s F-10 has been developed to support the growth of human diploid cells, white blood cells for chromosomal analysis, and primary explants of rat, rabbit, and chicken tissues. (sigmaaldrich.com)
The CCAC maintains approximately 7500 strains from around the world in four modern walk-in growth chambers operated at different temperatures. (uni-due.de)
Optimum growth temperature: 22° C. (carolina.com)
Leachates from a mine where the prevalence of pneumoconiosis was less also depressed growth of mouse cells but to a lesser extent. (cdc.gov)
Leachates from the more toxic mine in a 1/100 dilution depressed the growth rate below 50% of the growth of controls, and a 1/10 dilution further depressed the cells. (cdc.gov)

In addition, we investigated the effects of ADAMTS-5 in smooth muscle cell (SMC) apoptosis. (frontiersin.org)
The resulting damage cannot be overcome by the antioxidant system to protect the cell from oxidative damage, leading to necrosis, apoptosis, or autophagy of the target cell and tissue ( 10 , 11 ). (spandidos-publications.com)

Collectively, the results suggested that nanosilica exerts no effect on RANKL-induced osteoclast differentiation of RAW264.7 cells, although a detailed mechanistic examination of the nSP70-mediated cytotoxic effect is needed. (springer.com)

cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Which platforms can be used to expand cells for a bioprocess workflow? (corning.com)
The Leica DMi1 is designed for quick cell checks, for a smooth, fast workflow without time-consuming adjusting of the microscope. (leica-microsystems.com)
We aimed to assess how preanalytical variables (urine storage temperature, isolation workflow) affect diabetic kidney disease (DKD)-linked miRNAs or kidney-linked miRNAs and mRNAs (kidney-RNAs) in uEV isolates and to discover stable reference mRNAs across diverse uEV datasets. (lu.se)

The Quantikine Mouse IL-23 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-23 in cell culture supernates, mouse serum, and plasma. (rndsystems.com)

PBS' U-shaped bioreactors employ a patented mixing technology, the Air-Wheel™, that exploits the fact that cell cultures must be oxygenated. (genengnews.com)
Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK 293 or CHO cells, an expression or infection system and selection, followed by process and media optimization in small scale systems such as 96-well plates or bench-scale bioreactors. (beckman.com)
To meet volume demands, biopharmaceutical companies have built large-scale facilities with multiple 10,000 L (or even larger) cell culture bioreactors. (beckman.com)

After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
They are usually much more sensitive to culture conditions. (coleparmer.com)
The confluent cells will be incubated at 37 ± 1.5 °C and 5.0 ± 0.5 % CO2 (standard cell culture conditions). (europa.eu)
How to maintain cell culture conditions for enhanced sample safety. (the-scientist.com)
Cell culture refers to the process of growing cells under controlled conditions outside their natural environment. (integra-biosciences.com)

On the other hand, the glial cell line-derived neurotrophic factor (GDNF) has the strongest trophic effect on dopaminergic neurons (10). (sld.cu)
Our sensory systems are able to detect subtle changes in ambient temperature, due to the coordinated efforts of thermosensory neurons. (nih.gov)
Approximately 10-20 percent of ganglia neurons respond to cold temperatures, with thresholds for activation below 30°C [ 2 , 14-16 ]. (nih.gov)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Our data demonstrate that beryllium-stimulated bronchoalveolar lavage cells produce IL-10, and the neutralization of endogenous IL-10 does not increase significantly cytokine production, HLA expression, or T lymphocyte pro- liferation. (cdc.gov)

Therefore, the development of a method that allows screening of edited cells and minimizes cell manipulation and hands-on culturing would constitute a significant addition to the current genome engineering toolbox. (nature.com)
To treat patients with colorectal cancer, an approach that selectively targets cancer cells without damaging normal cells and which minimizes the risk of perforating the intestinal barrier is needed ( 2 ). (spandidos-publications.com)

Say goodbye to cell culture contamination nightmares and hello to happy cells. (coleparmer.com)
Cutting-edge reagents, kits, and techniques provide a robust solution to cell culture mycoplasma contamination. (the-scientist.com)

For over 80 years, Carolina has been providing the highest-quality living organisms and cultures available. (carolina.com)

The aim of this study is to learn if rat BMSC express brain-derived neurotrophic factor (BDNF) and glia-derived nerve factor (GDNF) and determine if this expression varies according to the number of culture passages. (sld.cu)
However, whether the efficacy of PDT depends on the expression level of p62 in colorectal cancer cell lines is not known. (spandidos-publications.com)
The present study aimed to examine the role of p62 expression levels in chlorin e6‑based PDT in colorectal cancer cells. (spandidos-publications.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)

A compilation of research experiences in stem cell, viral vector and vaccine production. (corning.com)
Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

You can place Petri dishes or cell culture flasks on the stage and monitor the specimen, characterize cells or tissues, and observe them in Brightfield or Phase contrast. (leica-microsystems.com)

3 An up-to-date list of WHO International Standards and Reference Reagents is available at http://www.who.int/bloodproducts/catalogue/en/ (accessed 28 February 2013). (who.int)

If you want to check or document cells expressing fluorescent proteins, we recommend the Leica DM IL inverted microscope. (leica-microsystems.com)
Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)

As of first of January 2022, StemTherapy and MultiPark have decided to merge the former iPSC, CRISPR and vector platforms into the new Cell and Gene Therapy Core . (lu.se)

Irradiated Ce6 produced singlet oxygen which rapidly induces tumor cell death. (spandidos-publications.com)
IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. (rndsystems.com)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)

This system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications. (stemcell.com)

BIOPHARM: Adherent cell culture is well established, and researchers are familiar with plates and flasks, but they don't scale to production. (corning.com)

The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)

The responsibility for the maintenance and development of the CCAC was taken over by the UDE from the University of Cologne (formerly Culture Collection of Algae at the University of Cologne ), the relocation of the collection to Essen was completed in January 2020. (uni-due.de)
Each green algae culture contains enough material for a class of 30 students. (carolina.com)

Scientists identify floral temperature patterns as a sensory cue that may help bees identify flower species. (the-scientist.com)

SDTT is hypothesized to be caused by size differences in the temperature dependence of underlying physiological processes that operate at the cellular and organ/system level (physiological SDTT). (bvsalud.org)
Because these constraints are temperature-independent, hydration water does not follow the super-Arrhenius temperature dependence of bulk water. (lu.se)

According to Daniel Giroux, vp of R&D, duplicating the agitation mechanisms of small reactors in large systems is problematic without subjecting cells to significant shear damage. (genengnews.com)

Infection with the encapsulated yeast Cryptococcus neoformans can result in harmless colonization of the airways, but it can also lead to meningitis or disseminated disease, especially in persons with defective cell-mediated immunity. (medscape.com)
Sometimes arterial sticks are done to get blood culture (testing for infection) or blood chemistry samples. (medlineplus.gov)

1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)

Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
The cellular portion is made up mainly of red blood cells, but it also includes white blood cells and platelets . (medlineplus.gov)

Anatomy15

Organisms16

Diseases4

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment52

Psychiatry and Psychology2

Phenomena and Processes112

Disciplines and Occupations7

Technology, Industry, Agriculture8

Information Science7

Health Care39

Geographicals4

Room temperature9

Mammalian cells2

Incubator11

Bioreactor5

Tissue culture17

Epithelial cell3

Assays2

Proliferation5

Differentiation of human pluripotent stem1

Fibroblasts2

Vivo3

Body temperature4

Incubators1

Vessel4

Embryonic5

Protein5

Substrate4

Biology6

Clonal5

Osmotic pressure1

Hybridoma1

Viability5

Vitro5

Bacteria2

Fluorescence2

Pluripotent4

Suspension9

Vessels4

Concentration5

Adherent cells3

Incubation2

Experiments8

Stimuli2

Range of temperatures2

Stains2

Assay1

Growth13

Apoptosis2

Cytotoxic1

Antibodies1

Workflow3

Serum1

Bioreactors3

Conditions7

Neurons4

Bronchoalveolar lavage1

Minimizes2

Contamination2

Living organisms1

Expression8

Stem6

Petri1

Reagents1

Proteins3

20221

Induces2

Laboratory1

Reproducible results1

Flasks1

Oxidative1

Algae2

Patterns1

Dependence2

Problematic1

Infection2

Infectious1

Cellular2