Because of its origin as the C-terminal end of smooth muscle myosin light chain kinase, it is called "telokin" (from a combination of the Greek telos, "end" and kinase). (wikipedia.org)
This enzyme has a pH optimum of 7.4 and temperature optimum of 30 °C. Telokin is an acidic protein with a PI value of 4-5 and 17-kDa with an amino acid sequence that is identical to the C terminus of the 130-kDa myosin light chain kinase (MLCK), although it is expressed as a separate protein and produced by an alternate promoter of the MLCK gene. (wikipedia.org)
A cell-specific promoter located in an intron of the smooth muscle myosin light chain kinase gene directs transcription of telokin exclusively in smooth muscle cells. (elsevierpure.com)
Promoter4
Telokin is transcribed from a second promoter, located within an intron, in the 3' region of the MLCK gene. (wikipedia.org)
Transgenic mice were generated in which a 310-bp rabbit telokin promoter fragment, extending from -163 to +147, was used to drive expression of simian virus 40 large T antigen. (elsevierpure.com)
Telokin expression levels correlate with the estrogen status of human myometrial tissues, suggesting that deletion of an estrogen response element (ERE) may account for the low levels of transgene expression driven by the 310-bp rabbit telokin promoter in uterine smooth muscle. (elsevierpure.com)
These data demonstrate that in addition to a minimal 310-bp proximal promoter at least one distal cis-acting regulatory element is required for telokin expression in uterine smooth muscle. (elsevierpure.com)
Gene1
Smooth muscle-specific expression of the T-antigen transgene paralleled that of the endogenous telokin gene in all smooth muscle tissues except uterus. (elsevierpure.com)
Smooth muscle2
Telokin (also known as kinase-related protein or KRP) is an abundant protein found in smooth-muscle. (wikipedia.org)
Telokin may play a role in the stabilization of unphosphorylated smooth-muscle myosin filaments. (wikipedia.org)
Isoforms3
Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. (wikipedia.org)
The two transcripts that produce the two telokin isoforms are the following: Isoform 7 This variant encodes the shorter isoform of kinase related protein, telokin. (wikipedia.org)
All isoforms including Telokin bind calmodulin. (icr.ac.uk)
Protein6
Telokin (also known as kinase-related protein or KRP) is an abundant protein found in smooth-muscle. (wikipedia.org)
Isoform 8 This variant encodes the longer isoform of kinase related protein, telokin. (wikipedia.org)
This enzyme has a pH optimum of 7.4 and temperature optimum of 30 °C. Telokin is an acidic protein with a PI value of 4-5 and 17-kDa with an amino acid sequence that is identical to the C terminus of the 130-kDa myosin light chain kinase (MLCK), although it is expressed as a separate protein and produced by an alternate promoter of the MLCK gene. (wikipedia.org)
Primary structure Telokin is an intracellular protein and, as such, does not contain the disulfide linkage between beta-strands B and F normally observed in the immunoglobulin constant domains. (wikipedia.org)
The characterization of telokin as a myosin-assembling protein successfully explained the discrepancy. (silverchair.com)
Homo1
The Km values of homo sapiens telokin is 0.018 mM at 23-25 °C and pH = 7.5. (wikipedia.org)
Bind1
Telokin has been shown to bind to unphosphorylated myosin filaments and to stimulate myosin mini-filament assembly in vitro. (wikipedia.org)
Smooth3
Telokin may play a role in the stabilization of unphosphorylated smooth-muscle myosin filaments. (wikipedia.org)
Because of its origin as the C-terminal end of smooth muscle myosin light chain kinase, it is called "telokin" (from a combination of the Greek telos, "end" and kinase). (wikipedia.org)
However, smooth muscle cells that are devoid of telokin have been observed. (silverchair.com)