• Reporter genes can be used to assay for the expression of a gene of interest that is normally difficult to quantitatively assay. (wikipedia.org)
  • Reporter gene assay have been increasingly used in high throughput screening (HTS) to identify small molecule inhibitors and activators of protein targets and pathways for drug discovery and chemical biology. (wikipedia.org)
  • Finally, recommendations are provided to guide the experimental design for germ cell mutagenicity data for regulatory submission, as well as other possible approaches to increase the reliability of the TGR assay. (nih.gov)
  • For luciferase or galactosidase enzyme activity cell lysates were analyzed by bioluminescence assays with Galacto-Star (Life Technologies) and Luciferase Reporter Assay kits (Promega), respectively. (labettor.com)
  • Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - BHK-21 baby hamster kidney cells using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells from Thermo Fisher Scientific. (labettor.com)
  • Genetic Luciferase Reporter System or Reporter gene assay has become an invaluable tool in studies of gene expression. (cdc.gov)
  • 1) protocol for expression and purification of the active JH receptor complex, 2) new and efficient ligand-binding assay for JH and related compounds, and 3) cell-based reporter system to measure JH-receptor mediated transcriptional activation by JH and related compounds. (europa.eu)
  • This assay was used to screen the human neuroblastoma cell line SH-SY5Y with a collection of adenoviral shRNAs and full-length human cDNAs totaling over 33,000 data-points," said Richard Janssen, senior director, BioFocus. (genengnews.com)
  • To systematically characterize the drivers of cell identity among the unique cell types, we are using a novel massively parallel reporter assay to quantify enhancer activity. (nih.gov)
  • If you need to monitor cell signaling pathway activity, our gene reporter assays support your research by providing rapid, sensitive and quantitative assessment of signal transduction pathway activation. (qiagen.com)
  • The assays measure the activities of downstream transcription factors, using either dual-luciferase or green fluorescent protein (GFP) reporter systems. (qiagen.com)
  • Available as single pathway assays or as multi-pathway arrays, our gene reporter assays for signaling pathway analysis allow you to monitor an individual pathway or obtain a comprehensive view of multiple pathways involved in a biological process. (qiagen.com)
  • Compounds are evaluated in cell-based assays for inhibition of viral replication and reduction of virus yields, plaques, or cytopathic effect. (nih.gov)
  • The compounds are tested with cell-based assays, and the resulting data are used to decide whether further studies in animal models of infection are warranted. (nih.gov)
  • Transwell assays were used to analyze the knockdown effects of hsa_circRNA_101996, miR-143, and overexpression of TET2 on cell proliferation, migration, and invasion abilities. (jcancer.org)
  • Binding interactions between, hsa_circRNA_101996 and miR-143 and between, miR-143 and TET2 were detected by Dual-luciferase reporter assays. (jcancer.org)
  • Estrogenic responses were determined using luciferase reporter assays and gene expression. (nih.gov)
  • Cell-based assays have become the rage once again, and there are a number of avenues for researchers to utilize to get the work they need done. (genengnews.com)
  • At Cambridge Healthtech's "World Pharmaceutical Congress," the focus was on new and improved technologies for cell-based assays for high-throughput screening (HTS). (genengnews.com)
  • The Cell-Based Assays for HTS track highlighted new screening tools and recent developments for cell-based assays, label-free screening, and novel approaches and technologies for cell-based assays and drug screening. (genengnews.com)
  • It will be important to have good cell assays, preferably based on human primary cells, to accommodate this kind of research. (genengnews.com)
  • Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [fr]. (wikipedia.org)
  • A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. (wikipedia.org)
  • Reporter genes can produce a protein that has little obvious or immediate effect on the cell culture or organism. (wikipedia.org)
  • The role of nuclear factor- κ B (NF- κ B) in regulating muscle protein degradation and expression of the ubiquitin-proteasome proteolytic pathway in response to a tumour cachectic factor, proteolysis-inducing factor (PIF), has been studied by creating stable, transdominant-negative, muscle cell lines. (nature.com)
  • Glucocorticoids also induce gene transcription and protein synthesis of the NF- κ B inhibitor, I κ B, and inhibit the expression of cytokines ( Almawi and Melemedjian, 2002 ). (nature.com)
  • The FOXL2 gene provides instructions for making a protein that attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • Before birth and in adulthood, the FOXL2 protein regulates the growth and division (proliferation) of hormone-producing ovarian cells called granulosa cells. (medlineplus.gov)
  • The FOXL2 protein also plays a role in controlled cell death (apoptosis) in the ovaries. (medlineplus.gov)
  • The FOXL2 gene mutations that cause primary ovarian insufficiency lead to a reduction in protein function, preventing the FOXL2 protein from controlling genes that regulate various activities in the ovaries. (medlineplus.gov)
  • Now, one approach involves giving sickle cell patients' cells a new gene to compensate for the defective one, to make their bodies produce a healthy version of a protein called hemoglobin, and that's what red blood cells need to carry oxygen in the body. (wunc.org)
  • deoxyuridine labeling and fate mapping with enhanced green fluorescence protein controlled statically by the Sftpc promoter or conditionally by the Scgb1a1 promoter revealed increased Sftpc and Abca3 mRNA seen on Day 4 reflected an increase in expansion of type II cells shortly after birth. (nih.gov)
  • Here, we used a global proteomic approach based on high-resolution mass spectrometry to compare the extracellular and intracellular roles of IL-33 in primary human endothelial cells, a major source of IL-33 protein in human tissues. (nature.com)
  • During inflammation, IL-33 is processed in the central activation domain by inflammatory proteases from mast cells and neutrophils, that generate mature forms of the protein with 10 to 30 fold higher biological activity 29 , 30 . (nature.com)
  • Furthermore, the expression of TNF-α and RANTES, as well as the phosphatidylinositol-3-kinase (PI3K)/protein kinase B(Akt)/inhibitory κB kinase (IKK) signaling pathway have been proven to be involved in the ADM of acinar cells. (spandidos-publications.com)
  • These errors result in protein variants with extended C termini 7 - 9 , generating a heterogeneous protein-length population from a single gene. (biorxiv.org)
  • Roadmap to IND for Protein and Peptide Therapies Cell and Gene Therapy Product Development Matrix. (nih.gov)
  • This gene encodes a melanocortin receptor-interacting protein. (nih.gov)
  • The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. (cancerindex.org)
  • The genome contains two RNA components--RNA-1, which encodes the virus's polymerase and the viral protein coat, and RNA-2, which encodes the movement protein that allows cell-to-cell infection. (the-scientist.com)
  • What does this gene/protein do? (cancerindex.org)
  • Recently, we reported highly active transforming growth factor (TGF)-β and bone morphogenetic protein (BMP) signaling in human chondrosarcoma samples and concurrent downregulation of paternally expressed gene 10 (PEG10). (cancerindex.org)
  • Analyses on gene structure and protein motif revealed that members from the same subgroup shared similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. (mdpi.com)
  • CFP expression was characterized using morphometric methods and immunohistochemistry with antibodies to neurofilament light (NF-L), neuronal nuclei (NeuN), POU-domain protein (Brn3a) and calretinin, which immunolabel ganglion cells, and syntaxin 1 (HPC-1), glutamate decarboxylase 67 (GAD 67 ), GABA plasma membrane transporter-1 (GAT-1), and choline acetyltransferase (ChAT), which immunolabel amacrine cells. (molvis.org)
  • Use green fluorescent protein, or GFP, and the cells will light up. (neurosciencenews.com)
  • BioFocus DPI in conjunction with the High Q Foundation developed a high-content screen to identify genes that modulate the survival of cells over-expressing mutant forms of the Huntington's protein. (genengnews.com)
  • This microscopic image of fibroblast cells shows the induction of cell fusion by a newly described gene and its protein, called myomerger. (cosmosmagazine.com)
  • In this image, a newly described gene and its protein called myomerger induce cell fusion amongst fibroblast cells - cells in connective tissue responsible for maintaining structural integrity. (cosmosmagazine.com)
  • We identified 42 genes affected by Wfs1 genotype, 10 genes regulated by VPA treatment, and 9 genes whose regulation by VPA was dependent on genotype. (hindawi.com)
  • and epigenomic editing with CRISPR/Cas9 to change gene regulation with high precision. (nih.gov)
  • These technologies provide valuable observational data but do not identify the functional connections within networks or explain the mechanism of gene regulation. (genengnews.com)
  • The role of liver gene regulation is rapidly being appreciated as part of the underlying processes that result in the observed pathobiology. (kent.edu)
  • Regulation of gene expression often involves sending a signal from the cell surface to deep in the nucleus. (kent.edu)
  • Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. (wikipedia.org)
  • Proteolysis-inducing factor induced degradation of I- κ B α , nuclear accumulation of NF- κ B and an increase in luciferase reporter gene activity in myotubes containing wild-type, but not mutant, I- κ B α proteins. (nature.com)
  • We found that exogenous extracellular IL-33 cytokine induced expression of a distinct set of proteins associated with inflammatory responses in endothelial cells. (nature.com)
  • Stop codon miscoding events give rise to longer proteins, which may alter the protein's function and thereby generate short-lasting phenotypic variability from a single gene. (biorxiv.org)
  • Applicants seeking support for the discovery and development of biotechnology products and biologics (e.g., peptides, proteins, oligonucleotides, gene therapies, and cell therapies) are encouraged to consider the new Blueprint Neurotherapeutics Network for Biologics (BPN-Biologics) program. (nih.gov)
  • To address these data gaps, the Germ Cell workgroup of the Genetic Toxicology Technical Committee of the Health and Environmental Sciences Institute reviewed the available TGR mutagenicity data in male germ cells, and, characterized the exposure history of tubule germ cells for different sampling times to evaluate its impact on germ cell mutagenicity testing using TG 488. (nih.gov)
  • Here we describe the Genetic Luciferase Reporter System as the method for detecting alteration of gene expression in response to external stimuli (e.g., nanoparticle s). (cdc.gov)
  • Acoustic reporter genes are genetic constructs that can be inserted into bacteria to direct them to make gas-filled nanostructures called gas vesicles. (nih.gov)
  • We've been hearing a lot of reporting recently about using the latest genetic technology to try and treat sickle cell disease. (wunc.org)
  • NPR health correspondent Rob Stein is in our studios to talk about the latest news about experimental genetic treatments for sickle cell disease. (wunc.org)
  • STEIN: And a genetic defect causes these red blood cells to become defective sickle-shaped cells, and they're really bad at carrying oxygen. (wunc.org)
  • Now, gene therapy is the first strategy scientists developed to try to treat diseases at the genetic level. (wunc.org)
  • It usually involves using harmless viruses to ferry new genes or genetic information into the body. (wunc.org)
  • Genetic information is information about genes, gene products, or inherited characteristics that may derive from an individual or family member (1). (cdc.gov)
  • Cells expressing the desired genetic behavior glow orange on the screen. (diagnosticimaging.com)
  • It opens up genetic manipulations in a much larger number of specific cell types and saves a huge amount of time and money," said Cepko. (neurosciencenews.com)
  • Genetic engineering of human embryonic stem (ES) cells is important for their application as tools for basic research and therapeutic development. (ca.gov)
  • Genetic modification is critical for achieving the full potential of human embryonic stem (ES) cells as a tool for therapeutic development and for basic research. (ca.gov)
  • Thus, human ES cell lines adapted for enzymatic passage and efficient clonal expansion can be highly amenable to genetic modifications, which will facilitate their application in basic science and clinical development. (ca.gov)
  • For instance, genetic defects in the HNF4 gene are associated with maturity-onset diabetes of the young (MODY1) and late-onset non-insulin-dependent diabetes mellitus (NIDDM). (kent.edu)
  • Retargeting of primary human T cells against CD19+ B cell leukemia using nonviral genetic modification are also being investigated. (mdanderson.org)
  • Enhancers are non-coding genetic elements that regulate transcription and are overwhelmingly responsible for cell type-specific gene expression. (nih.gov)
  • Harmine analogs are able to induce adult human beta-cell cycle entry at rates that are in the physiologic and potentially therapeutic range," say Dr Wang and colleagues. (medscape.com)
  • There remains an urgent need for antidiabetic agents that can induce regeneration and expansion of adult human beta cells in vivo or ex vivo. (medscape.com)
  • and evidence that Met interacts with the circadian clock genes to induce exit from a reproductive diapause (Bajgar et al. (europa.eu)
  • 1. Ex Vivo Radiolabeling and In Vivo PET Imaging of T Cells Expressing Nuclear Reporter Genes. (nih.gov)
  • 4. Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo. (nih.gov)
  • 19. Metabolic radiolabeling and in vivo PET imaging of cytotoxic T lymphocytes to guide combination adoptive cell transfer cancer therapy. (nih.gov)
  • In reality, in vivo imaging does not generate enough resolution to delineate individual cells, much less the activity of nanometric structures inside their nuclei. (diagnosticimaging.com)
  • Gene sets identified with oncogene cooperativity analysis regulate in vivo growth and survival of leukemia stem cells. (rochester.edu)
  • Development of positron emission tomography (PET) reporter variants: Development of d ifferential PET imaging reporters to simultaneously image dual population interactions in vivo. (mdanderson.org)
  • Clinical imaging of adoptively transferred T cells expressing chimeric antigen receptors is critical to understanding the distribution, targeting, and persistence of the cells in vivo. (mdanderson.org)
  • Studies are underway in primates and mice to image adoptively transferred T cells and their activation status in vivo. (mdanderson.org)
  • More than 260 mutations in the FOXL2 gene have been found to cause blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES). (medlineplus.gov)
  • It is difficult to predict the type of BPES that will result from the many FOXL2 gene mutations. (medlineplus.gov)
  • Five percent of mutations that cause BPES occur outside the FOXL2 gene in a neighboring region of DNA that normally controls the activity of the gene, known as a regulatory region. (medlineplus.gov)
  • Approximately 12 percent of mutations causing BPES are deletions involving the FOXL2 gene. (medlineplus.gov)
  • At least three mutations in the FOXL2 gene are thought to cause primary ovarian insufficiency without any other features of BPES. (medlineplus.gov)
  • Some gene mutations are acquired during a person's lifetime and are present only in certain cells. (medlineplus.gov)
  • Homozygous mutations in the WFS1 gene result in a rare disease-Wolfram syndrome that is characterized by early-onset diabetes mellitus , progressive optic atrophy, diabetes insipidus , and deafness [ 18 , 19 ]. (hindawi.com)
  • The frequency of heterozygous carriers of mutations in the WFS1 gene is remarkably high-1% of the general population [ 20 ] and heterozygosity for the WFS1 mutations has been reported to be a significant risk factor for psychiatric illnesses [ 20 , 21 ]. (hindawi.com)
  • Mutations in the WFS1 gene have been reported in patients with bipolar disorder, major depression, schizophrenia, and suicide victims without Wolfram syndrome [ 18 , 19 , 22 - 31 ]. (hindawi.com)
  • Mutations in this gene have been associated with familial glucocorticoid deficiency type 2. (nih.gov)
  • Mutations can be generated by gene targeting in order to assess the function of an endogenous gene in human ES cells or their derivatives. (ca.gov)
  • Stargardt disease (STGD) is a macular dystrophy caused by mutations in the ABCA4 (ABCR) gene. (arvojournals.org)
  • Marking of human embryonic stem (ES) and embryonal carcinoma (EC) cells with pluripotent promoter-driven reporter gene cassettes provides an important tool for studies related to maintenance of pluripotency, cell differentiation and cell selection. (nih.gov)
  • OCT4, TERF1 and telomerase reverse transcriptase component (TERT) are considered as pluripotent marker genes since they are expressed in both human ES and EC cells and significantly downregulated during the differentiation process. (nih.gov)
  • There are currently several ways to increase pancreatic beta cells that are being used as a treatment for type 1 diabetes: islet-cell transplantation and induction of differentiation of pluripotent stem cells to beta cells that are then reintroduced to patients. (medscape.com)
  • It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. (cancerindex.org)
  • Transforming growth factor-βs (TGF-βs) are pleiotropic cytokines implicated in control of cell growth, differentiation, inflammation, and apoptosis. (rupress.org)
  • A better understanding of basic biology is needed in key areas -such as developmental biology (particularly how the body exerts molecular control and the factors that influence differentiation into specific cell types) and immunology and immune cells. (nih.gov)
  • By introducing a fluorescent reporter gene into the Fezf2 gene via gene targeting, we will be able to monitor Fezf2 expression in the targeted cell line upon appropriate differentiation. (ca.gov)
  • Beta cells proliferate in humans during a brief temporal window beginning around birth, with a peak percentage (approximately 2%) engaged in the cell cycle in the first year. (medscape.com)
  • Originally, these radiolabeled nucleosides weren't developed for the purposes of suicide gene therapy but for imaging viral infection in humans,' said Dr. Peter S. Conti, director of the PET Imaging Science Center at the University of Southern California. (diagnosticimaging.com)
  • While extrapolations to humans require caution and further evaluation, our results suggest that lentiviral gene therapy is a potentially efficient tool for treating ABCA4-associated diseases. (arvojournals.org)
  • Yet, patterns are emerging by studying liver-specific genes in detail, such as the highly regulated gene for the cholesterol 7-hydroxylipase gene ( CYP7A1 ). (kent.edu)
  • 17. Repetitive noninvasive monitoring of HSV1-tk-expressing T cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU. (nih.gov)
  • 18. Noninvasive detection of tumor-infiltrating T cells by PET reporter imaging. (nih.gov)
  • Noninvasive and quantitative measures of the results of gene editing are needed. (nih.gov)
  • Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the interpretation of HTS data, novel coincidence reporter designs incorporating artifact suppression have been developed. (wikipedia.org)
  • This is achieved by linking the firefly luciferase gene to a promoter sequence. (cdc.gov)
  • We'll hook that up to a reporter gene that was the firefly luciferase. (medscape.com)
  • The knockdown of hsa_circRNA_101996 dramatically suppressed the cell migration, invasion, and proliferation of GC cells by sponging to absorb miR-143 and elevated the expression of TET2. (jcancer.org)
  • A drug that can drive the division and proliferation of pancreatic beta cells at human physiological rates has been identified from a screening of over 100,000 compounds, potentially paving the way for a breakthrough in the management of diabetes. (medscape.com)
  • Our idea was: Why don't we look at the promoter of the [oncogene] MYC , which we know is activated by basically everything that drives proliferation in any cell type? (medscape.com)
  • Of those 86 compounds, only harmine induced sustained division and proliferation of adult human beta cells in culture. (medscape.com)
  • Because hyperoxia inhibits cell proliferation, we hypothesized that it depleted the adult lung of type II cells by inhibiting their proliferation at birth. (nih.gov)
  • Phenotypic analysis of mutant embryos with all the techniques attached to this (in situ hybridization for gene expression, organotypic culture, proliferation and apoptosis, etc. (kpfu.ru)
  • By measuring the cell cycle distribution and apoptosis we can get more insight in the proliferative status ofsponge cells and we can compare different cultivation techniques, such as tissue culture vs. suspension cells. (wur.nl)
  • Receptors in this family with no known ligands are called orphan receptors and modulate gene expression. (kent.edu)
  • Although beta-cell expansion seems an obvious therapeutic approach, adult human beta cells have proven recalcitrant to such efforts, researchers explain. (medscape.com)
  • Molecular medical researchers no longer have to fly blind as they develop ways to navigate therapeutic genes or stem cells toward disease sites. (diagnosticimaging.com)
  • By harnessing the natural behavior of viruses and cells, molecular imaging illuminates the path of therapeutic genes and cells and serves as the guiding light in human clinical trials. (diagnosticimaging.com)
  • In the Nakauchi Lab, we are working on uncovering new diseases, elucidating the causes of disease, and developing therapeutic modalities by connecting the knowledge and methodology of basic science including immunology, molecular biology, cell biology, and developmental engineering with clinical medicine. (stanford.edu)
  • Type 1 and 2 diabetes affects 380 million people worldwide and both ultimately result from a deficiency of functional pancreatic insulin-producing beta cells. (medscape.com)
  • Gene network modeling via TopNet reveals functional dependencies between diverse tumor-critical mediator genes. (rochester.edu)
  • Accurate control of cell identity is integral to maintaining healthy, functional systems, and deviations can lead to developmental defects and diseases, including cancer. (nih.gov)
  • These results will yield the first functional enhancer map of human pancreas cells and advance our understanding of how these enhancers orchestrate the remarkable cell type-specific gene expression programs. (nih.gov)
  • Because of their simplicity and versatility, and because of the absence of endogenous luciferase activity in most cell types, this test can be applied for testing a large variety of nanomaterial s for their pathogenic or carcinogenetic effects on a wide range of mammalian cells. (cdc.gov)
  • MDCK cells were infected with serially diluted pseudovirus stock, and the infectivity reflected by the relative luciferase activity (RLA) was determined as the median (50%) tissue culture infective dose, according to the method of Reed and Muench. (cdc.gov)
  • Hyperenhancing stem cells carrying the reporter gene and accumulated probes can be observed as they arrive and begin to proliferate in targeted tissue. (diagnosticimaging.com)
  • Postmortem brain tissue from Parkinson's patients shows enhanced expression of several ARE-driven genes (NAD(P)H:quinone oxidoreductase 1and heme oxygenase-1), supporting the potential importance of Nrf2-ARE signaling in PD. (alzforum.org)
  • A critical step in regenerative medicine for people with muscular disorders is to figure out how to make stem cells fuse together to form working skeletal muscle tissue. (cosmosmagazine.com)
  • These genes are necessary for the creation of skeletal muscle tissue. (cosmosmagazine.com)
  • and iii) tubule germ cells at 28 + 28d produces reliable positive and negative results in both mice and rats. (nih.gov)
  • Researchers engineered genes that allowed bacteria in the bodies of mice to be detected with ultrasound. (nih.gov)
  • The scientists also showed that acoustic reporter genes could be used to monitor S. typhimurium bacteria after they were injected into tumors in mice. (nih.gov)
  • Tumor models were established by subcutaneous injection of tumor cells in Bl6/Rag2/GammaC double knockout mice. (jcancer.org)
  • Although it is unclear how neonatal oxygen affects development of the respiratory epithelium, alveolar simplification and depletion of type II cells has been observed in adult mice exposed to hyperoxia between postnatal Days 0 and 4. (nih.gov)
  • When mice were returned to RA, this expanded population of type II cells was slowly depleted until few were detected by 8 weeks. (nih.gov)
  • Aim of the present study was to compare the effect of chronic VPA treatment in wild type (WT) and Wfs1 knockout (KO) mice on hepatic gene expression profile. (hindawi.com)
  • Wild type, Wfs1 heterozygous, and homozygous mice were treated with VPA for three months (300 mg/kg i.p. daily) and gene expression profiles in liver were evaluated using Affymetrix Mouse GeneChip 1.0 ST array. (hindawi.com)
  • Among the genes that were regulated differentially by VPA depending on genotype was peroxisome proliferator-activated receptor delta ( Ppard ), whose expression was upregulated in response to VPA treatment in WT, but not in Wfs1 KO mice. (hindawi.com)
  • R-loops and DNA damage were also detected in mammary epithelial cells of mice treated with BP-3 and PP. (nih.gov)
  • Photomicrograph showing expression of the Lgr5-lacZ reporter gene in the base of small intestinal crypts in adult mice. (nih.gov)
  • Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. (cancerindex.org)
  • The most recognized phenotypic feature of STGD patients and the mouse model, the Abca4 -/- mice, is lipofuscin (A2E) accumulation in retinal pigment epithelium (RPE), considered to be both the earliest sign and the cause of RPE cell death at late stages of the disease. (arvojournals.org)
  • Since there is no treatment for STDG, we tested whether delivery of the normal (wt) human ABCA4 gene to the subretinal space of the Abca4 -/- mice via lentiviral vectors would correct the disease phenotype, i.e., reduce A2E accumulation. (arvojournals.org)
  • Toward this end, they engineered mice that expressed a reporter gene (human placental alkaline phosphatase) under the control of the ARE promoter. (alzforum.org)
  • In these mice, MPTP treatment increased reporter activity in the substantia nigra, consistent with Nrf2-mediated activation of protective genes in that PD-affected region. (alzforum.org)
  • Conversely, MPTP-mediated neuronal death disappeared in transgenic mice that overexpressed Nrf2-even when expression of the gene was restricted to astrocytes (by putting into Nrf2 knockout mice a Nrf2 transgene driven by an astrocyte-specific promoter). (alzforum.org)
  • 1 North East Institute for Stem Cell Research, Newcastle University International Centre for Life, Newcastle NE13BZ, UK. (nih.gov)
  • We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations. (nih.gov)
  • Applicants using stem cells for their therapy should check out NIH Stem Cell Information. (nih.gov)
  • Understanding the basic biologic functions of been a priority for the NIH, which supported the digestive system, which includes the GI visionary research leading to improved tract, pancreas, biliary system, and liver, is understanding of endoderm specification, of fundamental importance to the diagnosis patterning, stem cell kinetics, and crypt-villus and treatment of all diseases related to organization. (nih.gov)
  • Cancer and stem cell metabolism. (kpfu.ru)
  • One of the key points in Janssen's stem cell argument is their versatility. (genengnews.com)
  • Cell stem cell. (rochester.edu)
  • Our experience of gene targeting at Fezf2 also provided several valuable lessons for stem cell researchers who plan to apply gene targeting in their work. (ca.gov)
  • The HUES lines of human ES cells were developed for ease of manipulation and are gaining increased utility in stem cell research. (ca.gov)
  • Our ultimate goal is to contribute to establishing new frontiers of stem cell therapy and to make clinical applications of stem cells a reality. (stanford.edu)
  • Research fellow ( preferably self-funded) with research interests in stem cell biology, hematology, developmental biology and immunology fields are encouraged to contact us to further discuss specific projects available. (stanford.edu)
  • Optimization and standardization of tumor and stem cell transduction protocols. (mdanderson.org)
  • One of the more well-known reporter genes is herpes simplex virus type 1 thymidine kinase (HSV1-tk). (diagnosticimaging.com)
  • Ongoing studies aim to further evaluate the feasibility of HSV1-tk variants and phylogenic forms of thymidine kinase as PET reporters to achieve higher signal sensitivity and enhanced substrate specificity. (mdanderson.org)
  • The loss of reporter gene expression was partly reversed by addition of a histone deacetylase inhibitor and a demethylating agent, suggesting that in vitro methylation of these exogenous constructs and the epigenetic architecture around the site of integration are likely to play a major role in their transcriptional activity. (nih.gov)
  • AllStars Reporter Controls can be routinely transfected in experiments using reporter-gene constructs (such as plasmids carrying the reporter gene) to ensure optimal conditions are maintained. (qiagen.com)
  • Several genes are known to encode the gas-filled vesicles, which normally help water-dwelling bacteria regulate buoyancy. (nih.gov)
  • PEG10 counteracts signaling pathways of TGF-β and BMP to regulate growth, motility and invasion of SW1353 chondrosarcoma cells. (cancerindex.org)
  • In embryonic life and after early childhood, beta-cell replication is barely detectable. (medscape.com)
  • Gene targeting in a HUES line of human embryonic stem cells via electroporation. (ca.gov)
  • Our aim was to use core promoter regions from such pluripotent genes to drive expression of reporter genes that would be suitable for human ES cell selection amongst differentiated cells. (nih.gov)
  • In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. (wikipedia.org)
  • The AllStars Reporter Controls is intended for molecular biology applications. (qiagen.com)
  • 6. Molecular Imaging of Chimeric Antigen Receptor T Cells by ICOS-ImmunoPET. (nih.gov)
  • and molecular contrast agents that can be used to image reporter genes and to measure the outcomes of cell therapy in small animals. (nih.gov)
  • The extensive knowledge base within the Stratech team enables us to offer custom solutions for DNA cloning, gene cloning, gene mutagenesis, vector construction, PCR services and a wide range of molecular biology research needs. (stratech.co.uk)
  • Using molecular and cell culture techniques, we study the combinatorial effects of transcription factors on gene promoters using reporter genes. (kent.edu)
  • Neonatal hyperoxia stimulates the expansion of alveolar epithelial type II cells. (nih.gov)
  • These findings reveal that hyperoxia stimulates alveolar epithelial cell expansion when it disrupts angiogenesis. (nih.gov)
  • The projects seek to address critical needs, such as minimizing infection risk in dental environments, improving SARS-CoV-2 detection in saliva, and exploring mechanisms of viral entry into oral epithelial cells. (nih.gov)
  • Then, mRNA expression of Epithelial-mesenchymal transition (EMT) marker SNAIL and CYP enzymes were measured by PCR and determinate specific drug metabolites, associated with CYP enzymes by LC/MS. Our results demonstrated an epigenetic shift in HepG2 cells towards PHH after exposure to 5-AZA and Vitamin C which resulted in a higher expression and activity of specific drug metabolizing CYP enzymes. (cancerindex.org)
  • This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. (wikipedia.org)
  • Our study shows, that certain phase I genes and their enzyme activities are increased by epigenetic modification in HepG2 cells with a concomitant reduction of EMT marker gene SNAIL. (cancerindex.org)
  • Gradual loss of reporter gene expression was observed from 24 h post-transfection during transient transfection studies, while almost complete loss of reporter expression was observed upon stable transfections. (nih.gov)
  • Many methods of transfection and transformation - two ways of expressing a foreign or modified gene in an organism - are effective in only a small percentage of a population subjected to the techniques. (wikipedia.org)
  • The next step will be the transfection of juvenile sponges with reporter genes, such as GFP and luciferase. (wur.nl)
  • the reporter gene can be expressed constitutively (that is, it is "always on") or inducibly with an external intervention such as the introduction of Isopropyl β-D-1-thiogalactopyranoside (IPTG) in the β-galactosidase system. (wikipedia.org)
  • To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. (wikipedia.org)
  • The goal of project is to obtain stable and/or immortalized sponge cell lines required to harness this enormous potential through the use of novel biotechnological techniques such as RNA interference and CRISPR-Cas9 gene editing. (wur.nl)
  • In that quest, Steven A. Lommel of North Carolina State University in Raleigh and colleagues used a reporter gene to follow the viral activity in its host. (the-scientist.com)
  • In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. (wikipedia.org)
  • In acinar cells, alcohol has been proven to elevate the activation of transcription factors, such as nuclear factor-κB (NF-κB) and cytokine expression ( 5 ). (spandidos-publications.com)
  • It is well-known that epigenetics is strongly modified in tumor cells and that epigenetic regulators influence the expression and function of Cytochrome P450 (CYP) enzymes through altering crucial transcription factors responsible for drug-metabolizing enzymes. (cancerindex.org)
  • Our lab has identified candidate enhancer elements unique to the five major human pancreas cell types, and digital footprint analysis of chromatin accessibility data revealed that these regions are likely bound by cell type-specific transcription factors, underscoring the cell specificity. (nih.gov)
  • We have prioritized candidate enhancers which contain differentially enriched transcription factor motifs, and those containing motifs of additional highly cell type-specific transcription factors. (nih.gov)
  • Human immunodeficiency virus (HIV) and equine infectious anemia virus (EIAV)-derived lentiviral vectors were constructed, with the human ABCA4 gene under the control of constitutive (CMV, EF1α) or photoreceptor-specific (Rho) promoters. (arvojournals.org)
  • Although human liver tumor cells have reduced metabolic functions as compared to primary human hepatocytes (PHH) they are widely used for pre-screening tests of drug metabolism and toxicity. (cancerindex.org)
  • Optimization of standard protocols to stably and efficiently transduce tumor cells, T cells and hematopoietic stem cells. (mdanderson.org)
  • The AllStars Reporter Control should always result in high reporter-gene knockdown. (qiagen.com)
  • In contrast, knockdown of endogenous nuclear IL-33 expression using two independent RNA silencing strategies had no reproducible effect on the endothelial cell proteome. (nature.com)
  • In contrast, PEG10 knockdown augments the TGF-β1-induced motility of SW1353 cells. (cancerindex.org)
  • Development of retroviral and lentiviral vectors expressing reporter genes for PET, fluorescent and bioluminescent imaging. (mdanderson.org)
  • Studies are underway to develop lentiviral in-situ transduction methods to retarget T cells towards erbB2+ tumors in transgenic Her2NG and NSG mouse models. (mdanderson.org)
  • We have cloned thousands of candidate enhancers into lentiviral reporter libraries and are assessing activity of these elements in primary pancreas cells obtained from healthy donors. (nih.gov)
  • Similar vectors were made for the reporter gene (LacZ) to determine the transduction efficiency of retinal cells. (arvojournals.org)
  • Gene therapy caused some disasters early on, but it's finally started to work, and several experimental gene therapies are showing promise for sickle cell disease now. (wunc.org)
  • Developing new technologies to isolate, characterize, cultivate, and manipulate stem cells of the digestive system may provide new approaches to understand the pathogenesis and develop new therapies for digestive diseases. (nih.gov)
  • Ideally, MI should enable a radiologist to directly observe genes as they switch on or off in response to genetically based therapies. (diagnosticimaging.com)
  • The more we know about how cells function, the better we can understand disease and development, allowing us to design more effective therapies. (neurosciencenews.com)
  • For instance, faulty genes can be repaired using gene targeting before human ES cells or their derivatives are used in transplantation therapies. (ca.gov)
  • Because corticospinal neurons (more precisely their connection from the brain to the spinal cord) is often damaged in spinal cord injury, this research may one day lead to the development of cell transplantation therapies that would bring benefits to spinal cord injury patients. (ca.gov)
  • Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. (cancerindex.org)
  • The use of transgenic mouse technology to drive the expression of reporter molecules in neurons is rapidly becoming an important tool for visualizing neuronal populations and their neural circuitry. (molvis.org)
  • Extracellular IL-33 activates a growing number of target cells, including group 2 innate lymphoid cells, mast cells and regulatory T cells, but it remains unclear whether intracellular nuclear IL-33 has additional functions in the nucleus. (nature.com)
  • When it comes to understanding the function of the human genome and specifically how gene regulatory networks are controlled, SwitchGear's tools can be applied in a powerful way," noted Nathan D. Trinklein, cofounder and CEO of SwitchGear. (genengnews.com)
  • Its regulatory element libraries are built using luciferase reporter technology and are made available as ready-to-use tools for cell-based studies. (genengnews.com)
  • Protocol to use TopNet for gene regulatory network modeling using gene expression data from perturbation experiments. (rochester.edu)
  • Therefore, it is crucial to understand the regulatory mechanisms that drive cell type-specific functions. (nih.gov)
  • A specific somatic mutation in the FOXL2 gene has been found in a type of ovarian cancer that occurs in adulthood called adult granulosa cell tumor. (medlineplus.gov)
  • A mutation in this gene (−116C/G) is associated with bipolar disorder in the Japanese population [ 6 ] and XBP1 expression is reduced in patients with BD [ 7 , 8 ]. (hindawi.com)
  • PEG10 expression was suppressed by TGF-β signaling, and PEG10 interfered with the TGF-β and BMP-SMAD pathways in chondrosarcoma cells. (cancerindex.org)
  • Pathways as wide ranging as cholesterol synthesis, apolipoprotein gene expression, and cholesterol catabolism are coordinately regulated by mechanisms not clearly understood. (kent.edu)
  • In addition, recombinant reporter gene viruses are available as tools for antiviral screening. (nih.gov)
  • We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. (nih.gov)
  • Fluorescent reporter genes can be introduced by gene targeting into an endogenous gene to monitor gene expression and to trace cell lineages. (ca.gov)
  • STEIN: Scientists infused billions of her own cells that have been genetically modified with CRISPR into her body this summer to try to help her, and just recently, doctors reported the first hints that it looks promising. (wunc.org)
  • Our results showed that the choice of cell line used may have a profound effect on the ability of the human ES cells to be genetically manipulated by gene targeting. (ca.gov)
  • The drug screening team tests and characterizes novel compounds, drugs, and antibodies against numerous viruses in multiple cell lines under a variety of conditions. (nih.gov)
  • RNA BEGINNINGS Research that began as a quest to see how plant cells become infested with viruses ended in questions about the origins of life. (the-scientist.com)
  • While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. (cancerindex.org)
  • To increase the amount of plasmidic material, we performed transformations with DG1 E. coli competent cells from Delphi Genetics . (igem.org)
  • Cells were transfected with the indicated plasmids with Metafectene (Biontex, Germany) according to the manufacturer's instructions, cultured for an additional 24 hours. (labettor.com)
  • It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest. (wikipedia.org)
  • Thus, a method for identifying those few successful gene uptake events is necessary. (wikipedia.org)
  • Other varieties of reporter probes were designed to abet phosphorylation and/or uptake into the cell. (diagnosticimaging.com)
  • To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. (wikipedia.org)
  • Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions. (wikipedia.org)
  • In building the DNA construct, a segment of DNA coding for a flexible polypeptide linker region is usually included so that the reporter and the gene product will only minimally interfere with one another. (wikipedia.org)
  • During experimental setup using a reporter-gene construct, a reporter control siRNA can be used to determine optimal conditions. (qiagen.com)
  • When using a reporter-gene construct, a reporter control siRNA transfected in every experiment will indicate if conditions become suboptimal. (qiagen.com)
  • 2013 Human dendritic cells transfected with a human papilloma virus-18 construct display decreased mobility and upregulated cytokine production. (kpfu.ru)
  • The adenovirus transduces the reporter gene construct into cells, which transcribe the reporter genes into messenger RNA (mRNA) in the nucleus. (diagnosticimaging.com)
  • Luciferase reporter system for studying the effect of nanoparticle s on gene expression. (cdc.gov)
  • and an mRNA/lipid nanoparticle platform for CAR T cells). (nih.gov)
  • Specifically, gene targeting, the technology to modify an endogenous gene by homologous recombination, has many potential applications in human ES cells. (ca.gov)
  • The team at the Icahn School of Medicine conducted a series of experiments on four cell lines - rat insulinoma cells, mouse insulinoma cells, human hepatoma cells, and human colon-cancer cells - as well as human islet cells from 63 donors and rat and mouse islets. (medscape.com)
  • The enhancing of liver specific functions in hepatoma cells using epigenetic modifiers opens new opportunities for the usage of cell lines as a potential liver in vitro model for drug testing and development. (cancerindex.org)
  • The team found a combination of genes that could make E. coli bacteria produce gas vesicles on their own. (nih.gov)
  • The team next looked at whether they could monitor E. coli with the acoustic reporter genes using ultrasound in living mammals. (nih.gov)
  • We transformed DG1 E.coli competent cells with this construction. (igem.org)
  • Human ES and EC cells were stably transfected with a number of TERT, OCT4 and TERF1 promoter-driven EGFP or NTR gene cassettes. (nih.gov)
  • The human proteasome C3 subunit promoter contains elements homologous to the consensus NF- κ B-binding site ( Du et al, 2000 ), suggesting that NF- κ B may be involved in gene transcription. (nature.com)
  • So we're trying to figure out ways to direct it specifically to human pancreatic beta cells. (medscape.com)
  • Moreover, the team demonstrated that, in three murine and human islet models of diabetes, harmine not only increased the number of beta cells but also increased islet mass and improved glycemic control. (medscape.com)
  • 2. Imaging T Cell Dynamics and Function Using PET and Human Nuclear Reporter Genes. (nih.gov)
  • You can work with a large range of human primary cells, but the problem is that not all cell types are available in sufficient amounts. (genengnews.com)
  • However, unlike their mouse counterpart, the technology of gene targeting in human ES cells is not well established. (ca.gov)
  • Few reports have been published on successful gene targeting in human ES cells. (ca.gov)
  • In this paper, we described successful gene targeting of the Fezf2 gene in the HUES-9 human ES cell line. (ca.gov)
  • This will allow us to develop a more efficient protocol to differentiate human ES cells towards the corticospinal neurons in future. (ca.gov)
  • We found that the HUES-9 human ES cell line has a high tolerance for enzymatic passage and a high clonal expansion capability, which give this cell line a distinct advantage for gene targeting procedures that require single cell dissociation and electroporation. (ca.gov)
  • This observation has broad implications as it was previously thought that human ES cells differ from their mouse counterparts due to poor survival after enzymatic dissociation. (ca.gov)
  • This is the first report on gene targeting frequency for a gene not expressed in human ES cells using a strategy that is applicable to all human genes. (ca.gov)
  • Finally, HUES-9 cells can undergo gene targeting procedures without apparent loss of their characteristic features as human ES cells, which is critical for their widespread utility in basic and clinical research. (ca.gov)
  • Targeted modifications in human ES cells have met with limited success because of the unique culture conditions for many human ES cell lines. (ca.gov)
  • We tested conditions for gene targeting via electroporation in the HUES-9 human ES cell line and demonstrate here successful gene targeting at the gene encoding Fezf2 (also known as Fezl), a transcription factor involved in corticospinal neuron development. (ca.gov)
  • With a targeting strategy involving positive and negative selection that is applicable to all genes, we observed a gene targeting frequency of approximately 1.5% for Fezf2, a gene not expressed in human ES cells. (ca.gov)
  • Astrocytes outnumber neurons in the human brain, and research suggests that problems with these star-shaped glial cells may underlie the massive cell death in various neurodegenerative diseases. (alzforum.org)
  • Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene. (cancerindex.org)
  • An example of a selectable marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, which confers resistance to the antibiotic chloramphenicol. (wikipedia.org)
  • In the case of selectable-marker reporters such as CAT, the transfected population of bacteria can be grown on a substrate that contains chloramphenicol. (wikipedia.org)
  • HUES-9 cells exhibit an intrinsically high efficiency of clonal expansion and sustain electroporation-based gene targeting procedures without any significant loss of pluripotency marker expression or karyotypic stability. (ca.gov)
  • Thus, the 28 + 28d regimen may provide an approach for simultaneously assessing mutagenicity in somatic tissues and germ cells from the same animals. (nih.gov)