• Our research is dedicated to the elucidation of structure and functional mechanism of biomolecules ranging from RNA and RNA-protein complexes via large soluble protein complexes to membrane proteins. (uni-frankfurt.de)
  • Non-covalently bound complexes, which can include membrane and soluble proteins as well as nucleotides and ligands, play an essential role in many cellular processes. (europa.eu)
  • The determination of the plasma membrane (PM) proteome, resolution of membrane protein topology, establishment of numerous receptor protein complexes, identification of ligand-receptor pairs, and elucidation of signalling networks originating at the PM have all benefited from recent developments in proteomic mass spectrometry. (alliedacademies.org)
  • Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. (thermofisher.com)
  • During his PhD, Jose studied the structure, folding properties and interactions of the SH3 domains of the CD2 adaptor protein using NMR as the main technique and was awarded an EMBO short-term fellowship to develop new NMR methodologies for the study of weak protein complexes in the laboratory of Dr Martin Blackledge at the Institut the Biologie Structurale (Grenoble, France). (kent.ac.uk)
  • CrMP-Sol also presents QTY-enabled water-soluble designs to facilitate native membrane protein studies despite natural hydrophobicity. (mit.edu)
  • The key for this approach is the appropriate selection of quenchers, which should be neutral, water-soluble and biocompatible. (biologists.com)
  • 1974. Characteristics of dispersions and water-soluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. (cdc.gov)
  • Argonne biologists Philip Laible and Deborah Hanson developed a system that uses photosynthetic bacteria ( Rhodobacter ) to create much larger amounts of membrane protein that was ever before available, driving down production costs and making studies of membrane proteins easier both in terms of scalability and purification. (rdworldonline.com)
  • Our protein characterization platform is located on the Center for Structural Systems Biology (CSSB) and connected to our HTX facility by a science bridge. (embl.org)
  • The structural diversity of sPLA 2 -BPs reveals sPLA 2 s as very promiscuous proteins, and we offer some structural explanations for this nature that makes these proteins evolutionarily highly advantageous. (ijbs.com)
  • Binding to a receptor triggers a very rapid structural change, with some parts of the protein flipping through 180°, pulling the viral membrane and the host membrane close together to allow them to fuse. (nature.com)
  • Further structural modification was needed to achieve stability, whereas fusion proteins clamped using the gp41 fragments were stable on their own. (nature.com)
  • Unfortunately, structural information for membrane proteins is exceedingly scarce. (rdworldonline.com)
  • While established technology can routinely generate sufficient quantities of soluble protein for structural studies, no general systems exist or are routinely employed which are tailored specifically for the overexpression of membrane proteins. (rdworldonline.com)
  • The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. (thermofisher.com)
  • Jose joined the School of Biosciences in September 2016 as a Lecturer in Biological NMR and head of the NMR facility, where his research group specialises in the structural and functional studies of proteins able to insert in lipid membranes, forming pores and channels. (kent.ac.uk)
  • We study their mechanism of membrane insertion, the factors that trigger their activation and their inhibition using integrative structural biology tools, with a particular emphasis in solution NMR, as well as biophysics and fluorescence microscopy. (kent.ac.uk)
  • Important physiological processes, such as receptor-ligand interactions, lipid flip-flops and lipid-raft formation, are accompanied with the motions and structural changes of biomolecules on plasma membranes. (biologists.com)
  • The company's droplet-based microfluidic screening technology aims at the fast generation of antibodies against target molecules presented on cellular membrane surfaces or as soluble protein. (embl-em.de)
  • These include the generation of a variety of lipid mediators, along with membrane remodelling, modification of extracellular non-cellular phospholipid components of pulmonary surfactant, microparticles and lipoproteins, and degradation of microbial membranes and dietary phospholipids. (ijbs.com)
  • Proteins, glycolipids, and phospholipids make up cellular membranes, which are crucial for protecting and limiting metabolic processes within cells while also preserving cellular integrity and homeostasis. (alliedacademies.org)
  • Stomatin, prohibitin, flotillin, and HflK/C (SPFH) domain proteins are found in the lipid raft microdomains of various cellular membranes. (rcsb.org)
  • Our data provide proof-of principle of a new approach for directing small molecule inhibitors to disease causing RAFT targets in cellular membranes. (healthnewstrack.com)
  • Longevity.Technology: Cellular senescence - the zombie state in which cells forget to die - is one of the nine hallmarks of aging . (longevity.technology)
  • MLKL is a critical mediator of necroptosis that ruptures cellular membranes leading to cell death. (kent.ac.uk)
  • In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)
  • Cellular proteins and organelles are engulfed into a double-membrane vesicle to form an autophagosome. (spandidos-publications.com)
  • Both are based on four basic principles: biocompatibility, maintenance of adequate space to support the newly formed tissue, the promotion of growth of osteogenic cells and peripheral sealing to prevent growth of unwanted cellular tissue inside the defective area and the displacement of the membrane 3-5 . (bvsalud.org)
  • This includes for example the screening of potential drugs against Alzheimer's disease, or the development of handling procedures that allow the investigation of membrane proteins in dependence of the lipids which compose the cell membranes. (europa.eu)
  • Transmembrane proteins are water insoluble, which makes them difficult to study using mass spectrometry and conventional biochemical methods, but not impossible (MS). In this review, we emphasise the importance of shotgun MS for hastening the discovery and investigation of membrane proteins. (alliedacademies.org)
  • Membrane-tethered ligands are recombinant proteins comprised of an extracellular peptide ligand, a linker sequence, and an anchoring transmembrane domain. (nih.gov)
  • Native and redesigned proteins exhibited highly similar characteristics, predicted structures and binding pockets, and slightly different docking poses against known ligands, although task-specific designs are still required for proteins more susceptible to internal hydrogen bond formations. (mit.edu)
  • Secreted phospholipases A 2 (sPLA 2 s) participate in a very broad spectrum of biological processes through their enzymatic activity and as ligands for membrane and soluble receptors. (ijbs.com)
  • This 55 kDa protein representing the extracellular portion of gp80 is generated by shedding, a process that seems to be controlled by protein kinase C. It is still functional, indicating that soluble gp80 plays a biological role in promoting IL-6 activity. (thermofisher.com)
  • The authors then went on to investigate whether queenFRET can monitor interactions of extracellular proteins with plasma membranes. (biologists.com)
  • Role of neutrophil extracellular la NETosis en las enfermedades infecciosas pulmonares. (bvsalud.org)
  • In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. (nature.com)
  • Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
  • Although protein-protein interactions (PPIs) are promising targets, drugging them is one of the key challenges in drug discovery 19 . (nature.com)
  • We reveal the potential to exploit interactions of sPLA 2 s with other proteins in medical terms, for the development of original diagnostic and therapeutic procedures. (ijbs.com)
  • To better understand membrane protein structure, protein-protein interactions, and signalling networks that emerge from the membrane surface, we briefly discuss recent MS developments to determine the entire membrane proteome. (alliedacademies.org)
  • Non-specific fluorescence background can have several causes, including sample autofluorescence, non-specific antibody binding, and electrostatic interactions of fluorescent dyes with proteins and blotting membranes. (biotium.com)
  • Here we exploit optogenetics to experimentally change the kinetics of protein-protein interactions on demand. (optobase.org)
  • We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)
  • Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
  • Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells in a variety of tissues. (biotium.com)
  • The model enzymes include soluble methane monooxygenase (sMMO) that oxidizes methane to methanol and integral membrane desaturases (IMD) that oxidatively transform fatty acids to desaturated and hydroxylated lipids. (auburn.edu)
  • These may be different molecules within the cells like proteins, polysaccharides, or nucleoproteins and may also be the whole cell, like a tumor cell or organisms like bacteria, viruses, fungi, parasites, or agents containing genetic material such as nucleic acids or lipids. (medscape.com)
  • Α-klotho is a transmembrane protein, integral to the membrane of the cell and spanning its entirety. (longevity.technology)
  • Integral membrane proteins and membrane-associated proteins are essential players in biological processes. (kent.ac.uk)
  • Antibodies can be found on the surface of lymphocytes as an integral part of the cell membrane protein or can be freely circulating in the blood or be part of one of the body's gland secretion. (medscape.com)
  • biorefinery process, through membrane technology, producing an extract enriched in soluble proteins, free from the insoluble protein fraction, with a low lipid content and eliminating the colored chlorophyll-a. (unl.pt)
  • 3M Emphaze AEX Hybrid Purifier is the newest single-use technology for polishing stage clarification of mammalian cell culture fluid and is designed to reduce both soluble and insoluble impurities. (3m.com.au)
  • It turns out the brain is loaded with such stuff, both in lipid membranes and tiny water-free ("hydrophobic") lipid-like pockets within certain brain proteins. (edge.org)
  • A parallel approach may be applied to developing therapeutics targeting other peptide hormone G protein-coupled receptors. (nih.gov)
  • The chemerin receptor (CMKLR1) is a G protein-coupled receptor found on select immune, epithelial, and dorsal root ganglion/spinal cord neuronal cells. (nih.gov)
  • The SARS-CoV-2 spike or fusion protein is a trimer: a group of three units, each consisting of a receptor-binding subunit, S1, and a subunit responsible for fusion, S2. (nature.com)
  • IL-6 exerts its action via a cell surface receptor which consists of two subunits, an 80 kDa ligand binding subunit (gp80) of 468 amino acids and a 130 kDa signal transducing protein (gp130) of 896 amino acid residues. (thermofisher.com)
  • So far, the soluble IL-6 receptor is unique in acting as an agonist together with its ligand. (thermofisher.com)
  • The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. (thermofisher.com)
  • The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • For permeate, or proteins, ultrafiltration concentrates all soluble proteins and diafiltration can be used to wash out small molecules (sugars, salts, etc.) to increase the final protein purity. (pall.com)
  • Ultrafiltration operated in a diafiltration mode, under controlled permeate flux conditions, led to the highest soluble protein recovery (78%) with the highest constant permeate flux (12 L·m −2·h−1 ) and low membrane fouling. (unl.pt)
  • To expedite the development of long acting, stable chemerin analogs as candidate therapeutics, we used membrane-tethered ligand technology. (nih.gov)
  • Velabs Therapeutics, a recent startup company of the European Molecular Biology Laboratory (EMBL), is a pioneer in microfluidic technology for screening of antibodies with modulatory function on complex signal proteins like GPCRs or ion channels. (embl-em.de)
  • Velabs Therapeutics is a spin-out from Europe's most prestigious life science research and instrumentation institute, the European Molecular Biology Laboratory (EMBL), standing at the forefront of microfluidics-based screening technology and applications world-wide. (embl-em.de)
  • JADO Technologies GmbH, the leading developer of RAFT intervention therapeutics, announced the publication of a paper in Science demonstrating a potential novel strategy for therapeutic intervention in Alzheimer's and other diseases by targeting discrete sub-compartments in the cell membrane (RAFTS). (healthnewstrack.com)
  • CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. (thermofisher.com)
  • A soluble form of the human gp80 protein has been detected in serum and urine samples. (thermofisher.com)
  • The transport of serum amino acids into cells is an active process that is facilitated by plasma membrane-localized amino acid transporters. (spandidos-publications.com)
  • Using this technology, we established that a 9-amino acid-tethered chemerin fragment (amino acids 149-157) activates both mouse and human CMKLR1 with efficacy exceeding that of the full-length peptide (amino acids 21-157). (nih.gov)
  • This compound provides a prototype membrane-anchored peptide for the treatment of inflammatory disease. (nih.gov)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • amyloid peptide, can be inhibited effectively using compounds anchored to cell membrane RAFTS. (healthnewstrack.com)
  • Cell-mediated response was evaluated by measuring interferon (IFN)-γ levels by ELISA in plasma harvested from whole-blood stimulated with SARS-CoV-2 peptide pools, including spike (S), nucleocapsid (N) and membrane (M) proteins. (bvsalud.org)
  • For this, they labeled the human host peptide LL-37 with tetramethylrhodamine (TAMARA), and added the peptide to culture medium to monitor its membrane insertion dynamics in a lung epithelial carcinoma cell line. (biologists.com)
  • Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)
  • To enable in vivo delivery of a corresponding soluble membrane anchored ligand, we generated lipidated analogs of the 9-amino acid fragment. (nih.gov)
  • RÉSUMÉ L'objectif de l'étude était d'évaler l'importance clinique du ligand de CD40 soluble (sCD40L) chez des patients atteints d'un carcinome hépatocellulaire (CHC) associé au virus de l'hépatite C (VHC). (who.int)
  • POPC is also used in systems mimicking the cell membrane such as Nanodiscs. (wikipedia.org)
  • It is available commercially synthetically and is naturally present in eukaryotic cell membranes. (wikipedia.org)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • 3M™ Polisher ST is a fully encapsulated single-use anion exchange (AEX) product intended to reduce host cell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography. (3m.com.au)
  • Enveloped viruses such as SARS-CoV-2, the virus responsible for COVID-19, infect a host via proteins protruding from their surface that bind to and fuse with a target cell's membrane, allowing the virus to enter a cell. (nature.com)
  • The three S1 and S2 subunits form a fusion spike embedded in the viral membrane and held in a very particular conformation until it binds to a target cell. (nature.com)
  • The first focuses on spatial organization of proteins within a bacterial cell. (uwm.edu)
  • These posters to be presented at the AACR Meeting focus on the investigational nucleic acid platform, UNITE™ (UNiversal Intracellular Targeted Expression) for two vaccines, ITI-3000 for Merkel cell carcinoma (targeting the large T antigen of the Merkel cell polyomavirus) and Her2/Neu-LAMP DNA vaccine, both of which fuse a tumor associated antigen with lysosomal associated membrane protein 1 (LAMP-1). (biospace.com)
  • This proprietary lysosomal targeting technology results in enhanced antigen presentation and a balanced T cell response. (biospace.com)
  • CD154) is a transmembrane protein expressed on the surface of activated T cells, particularly on CD4 T cells, which stimulates CD40-dependent activation of antigen-presenting cells (APCs), resulting in enhancement of T cell and antibody responses. (biospace.com)
  • The application of ascorbic acid reduced cell membrane damage and increased catalase activity in water-stressed trees of both cultivars. (frontiersin.org)
  • secretase is internalized from the cell membrane into intracellular compartments (endosomes) where it cleaves its substrate, Amyloid Precursor Protein (APP). (healthnewstrack.com)
  • The cell membrane serves as the interface between an organism and its environment, and internal membranes in multicellular organisms separate functional compartments within cells. (rdworldonline.com)
  • The part of the protein that exists outside the cell can become detached or severed from the rest and released into the blood plasma, urine or cerebrospinal fluid as hormonal, soluble, circulating forms. (longevity.technology)
  • Their functions range from transport across the membrane, cell adhesion and regulation of the cell shape. (kent.ac.uk)
  • They represent around 40% of all proteins, and given their importance in the cell is not surprising that around 50% of the current drugs target membrane proteins. (kent.ac.uk)
  • Based on our expertise in medium to high-throughput screening approaches involving microbes and a background in yeast cell biology, we expand the knowledge on membrane-related processes and extend the molecular toolboxes for applications on industrial scale. (tugraz.at)
  • Despite various advanced fluorescence tools being available to study membranes in vivo , novel technologies are required to make it possible to measure conformational changes of biomolecules in real time in live cell plasma membranes. (biologists.com)
  • The positional changes of the fluorophore-labeled biomolecule in cell membranes can be reflected by its intensity or lifetime changes. (biologists.com)
  • The term pemphigus refers to a group of autoimmune blistering diseases of the skin and mucous membranes characterized histologically by intraepidermal blister and immunopathologically by the finding of in vivo bound and circulating immunoglobulin G (IgG) antibody directed against the cell surface of keratinocytes. (medscape.com)
  • Pemphigus vulgaris is an autoimmune, intraepithelial, blistering disease affecting the skin and mucous membranes and is mediated by circulating autoantibodies directed against keratinocyte cell surfaces. (medscape.com)
  • Finally, the further GIX sPLA 2 s are found in venom of marine snails, and GXIA and GXIB sPLA 2 s are plant proteins. (ijbs.com)
  • These fusion proteins are the basis of many subunit vaccines, a type in which a part of the pathogen being vaccinated against is injected to prompt the body to efficiently raise an immune response that can protect against the live virus. (nature.com)
  • The hydrophobicity, intricate post-translational modifications (PTMs), and low quantity of membrane proteins make it difficult to analyse them. (alliedacademies.org)
  • The identification of membrane proteins is now more accurate than in earlier analyses due to improved instrument performance, making it possible to analyse complicated protein mixtures in greater detail. (alliedacademies.org)
  • Focus includes discussions on wheat bran, distillers' dried grains-based within the biorefinery concept, and different techniques for the separation, extraction, recovery and formulation of valuable compounds, including proteins, arabinoxylans, and beta-glucan. (gambit-ksiazki.pl)
  • Membrane technologies for the fractionation of compounds recovered from cereal processing by-products 9. (gambit-ksiazki.pl)
  • Molecular techniques make this possible through the isolation of important genes and the production of biomedical compounds through recombinant technology. (noaa.gov)
  • An organic extraction is performed to remove proteins, fats, and other organically soluble compounds, leaving nucleic acids in the aqueous solution. (noaa.gov)
  • The frozen sample is later thawed at 4° C and homogenized in an extraction buffer that dissolves all the cell's membranes to release nucleic acids (DNA and RNA). (noaa.gov)
  • For albumins, ultrafiltration can be utilized to concentrate all soluble proteins followed by washing out small molecules (sugars, salts, etc.) to increase final protein purity. (pall.com)
  • Membrane technology via ultrafiltration can support the recovery of these losses in the light phase by concentrating all soluble proteins and washing out small molecules to increase the final product yield. (pall.com)
  • cells was processed by ultrafiltration using a membrane with a cut-off of 100 kDa MWCO. (unl.pt)
  • To foster plant terpenoid biosynthesis in yeasts, and to improve the industrial applicability of membrane-attached cytochrome P450 enzymes we have identified several endogenous helper factors in P. pastoris and S. cerevisiae. (tugraz.at)
  • The same conformational change occurs when the spike protein is isolated or made by recombinant technology to produce a soluble subunit vaccine. (nature.com)
  • This presentation reports results of preclinical mouse studies on a HER2/Neu-LAMP DNA vaccine that included a bicistronic DNA construct in which both the tumor antigen (HER2) fused with LAMP-1 (lysosomal- associated membrane protein 1) and sCD40L were expressed separately. (biospace.com)
  • Les concentrations sériques de sCD40L circulant et d'interleukine 10 circulante ont été analysées à l'aide de la méthode immuno-enzymatique chez 30 patients positifs pour le VHC avec un CHC, chez 30 patients patients positifs pour le VHC avec une cirrhose du foie, et chez 30 volontaires d'âge correspondant en bonne santé avec des anticorps anti-VHC négatifs servant de groupe témoin. (who.int)
  • Membrane proteins are critical mediators for tumor progression and present enormous therapeutic potentials. (mit.edu)
  • Most membrane proteins are less abundant and more hydrophobic than typical soluble proteins, which makes study of these proteins more challenging. (alliedacademies.org)
  • The enrichment of low-abundance membrane proteins at membrane and/or protein levels and the dissolution of hydrophobic membrane proteins have both been accomplished using a variety of established and recently developed techniques. (alliedacademies.org)
  • To make a long story short, Nicholas Franks and William Lieb at Imperial College in London showed in a series of articles in the 1980's that anesthetics act primarily in these tiny hydrophobic pockets in several types of brain proteins. (edge.org)
  • This process creates partial protein denaturation (potential structure modification) that may negatively impact solubilization of final protein powder and other properties like gelation, foaming, water retention, among others. (pall.com)
  • The authors used a vesicle-plasma membrane fusion method to incorporate fluorescent phosphatidylethanolamine into the membrane. (biologists.com)
  • The centers of both fluorescence peaks are consistent with the thickness of the plasma membrane. (biologists.com)
  • Here we revisit tau protein aggregation at primary, secondary, tertiary and quaternary structures. (ibecbarcelona.eu)
  • While other trimerization proteins, such as foldon from the T4-phage fibritin, were tried, the resulting structures were unstable and wouldn't keep well in storage. (nature.com)
  • Considering data availability first, it's notable that out of the many thousands of protein structures studied by NMR, only a tiny fraction have had the original datasets made available to other researchers. (bruker.com)
  • However, only a small number of structures of membrane proteins have been elucidated so far due to their low expression levels and the requirement of membrane mimetic environments for their study. (kent.ac.uk)
  • Except the first step of milk pasteurization with no impact on the protein native quality, Prolacta® is produced at low temperature and is close to its original form in milk, preserving its excellent biological value and amino acid profile. (lactalisingredients.com)
  • We offer services for protein quality control in addition to characterization techniques for interaction of biomolecules. (embl.org)
  • Nanopore sensing is an emerging technology for single-molecule analysis of biomolecules. (nature.com)
  • In previous work, the authors developed an in vitro fluorescence method to measure positions of membrane proteins in lipid bilayers, however this in vitro approach cannot be directly applied to study the trafficking of biomolecules in plasma membranes of live cells. (biologists.com)
  • In this chapter, we first define and classify the membrane separation technology and then comprehensively describe its applications, for instance, component separation, filtration, removal of bacteria, and wastewater treatment in dairy industry. (intechopen.com)
  • To help boost your biopharmaceutical manufacturing efficiency, 3M offers a robust portfolio of biopharmaceutical manufacturing products - from membranes and hybrid chromatographic purifiers to depth filtration systems and process hardware. (3m.com.au)
  • A significant rise in the representation of PM proteins in large-scale data sets has been attributed to a number of recent technological developments, including enhanced sample preparation, equipment, and liquid chromatographic (LC) performance [ 1 ]. (alliedacademies.org)
  • To activate antigen-specific CD4 T cells in vivo, the investigators utilized the nucleic acid vaccine platform, UNITE (UNiversal Intracellular Targeted Expression), which fuses a tumor-associated antigen with lysosomal-associated membrane protein 1 (LAMP1). (biospace.com)
  • Biorefinery strategies for upgrading distillers' dried grains with solubles 4. (gambit-ksiazki.pl)
  • We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
  • Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)
  • Here, we show that the latter genes encode carotenoid proteins (CTDHs). (nih.gov)
  • As a result of the rapid advances in genetics technology and the Human Genome Project, most of the estimated 100,000 genes in humans will be identified by the year 2005 (1). (cdc.gov)
  • From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)
  • Selective microfiltration also allows protein fractionation, which is the separation of the bigger proteins or larger proteins from the smaller proteins, for different end products. (pall.com)
  • The process proposed minimizes fouling phenomena at the membrane surface, making it possible to achieve high permeate fluxes, thus reducing the need for membrane cleaning and, therefore, contributing to an extended membrane lifetime. (unl.pt)
  • CMKLR1 is primarily coupled to the inhibitory G protein, Gαi, and has been shown to modulate the resolution of inflammation and neuropathic pain. (nih.gov)
  • secretase inhibitor to the membrane would confer inhibitory activity upon it by bringing it to the right compartment. (healthnewstrack.com)
  • We focus our studies on the chemotaxis systems within Pseudomonas aeruginosa , and seek to elucidate how protein localization is correlated to function. (uwm.edu)
  • Our work aims to elucidate how MLKL ruptures the membrane at molecular detail. (kent.ac.uk)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • Progress in biological NMR is hindered by data availability and processing time," says Prof. Dr. Roland Riek, Professor of Physical Chemistry and head of the Bio-NMR group at the Department of Chemistry and Applied Biosciences at the Swiss Federal Institute of Technology (ETH) Zürich. (bruker.com)
  • Effects of Outer Membrane Protein TolC on the Transport of Escherichia coli within Saturated Quartz Sands. (uwm.edu)
  • The FRET between the fluorophore and the quenchers results in a very steep change in fluorescence as the fluorophore-labeled molecule moves from the outer to the inner surface of the membrane or vice versa. (biologists.com)
  • Optogenetics is a cutting-edge technology that merges light control and genetics to achieve targeted control of tissue cells. (optobase.org)
  • The long funnel-shaped structure and nanofluidic characteristics of the YaxAB nanopore enable the electro-osmotic trapping of diverse folded proteins and high-resolution monitoring of PDIs. (nature.com)
  • Recently, the characteristics of a family of soluble carotenoid-binding proteins (Helical Carotenoid Proteins [HCPs]), paralogs of the N-terminal domain of OCP, were described. (nih.gov)
  • This family of proteins contains two subgroups with distinct characteristics. (nih.gov)
  • Antibodies belong to immunoglobulin family of protein and have 5 isotypes (G, A, M, E, D) with further subclasses of G and A. The classes are differentiated with the characteristics of the Ig heavy chain of the antibody. (medscape.com)
  • While development of The University of Queensland's first iteration of a COVID-19 vaccine will not progress, the process matured their rapid-response 'molecular clamp' vaccine technology. (nature.com)
  • Researchers involved in the development of the vaccine, produced using UQ's proprietary 'molecular clamp technology', were disappointed, but some aspects of the work gave them encouragement. (nature.com)
  • UQ's molecular clamp technology was designed to hold the spike protein in its original form to produce a useful subunit vaccine. (nature.com)
  • This was the impetus for building the molecular infrastructure to maintain a spike protein in its pre-fusion form. (nature.com)
  • In addition, we are also investigating the mechanism of partitioning chemotaxis proteins that regulate flagellar based motility. (uwm.edu)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • Here we have focused on identifying and characterizing CARs in prion-like proteins (pCARs) from plants, a lineage that has been poorly studied in comparison with other prionomes. (ibecbarcelona.eu)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • Even though the majority of membrane proteins cannot be accessed using conventional sample preparation methods, this is a subject that is very important to our discussion and has already been well reviewed. (alliedacademies.org)
  • Fluorescence imaging and spectroscopic techniques are often the methods of choice for investigating membrane proteins in live cells, however they face various hindrances. (biologists.com)
  • In other words, the original steps of separation via centrifugation and isoelectric precipitation are eliminated and instead, clarification is achieved via microfiltration using membranes. (pall.com)
  • Membrane separation technology being one of those also focused on a cost-effective and environment-friendly manner, which can be widely applied in dairy industry for many useful purposes. (intechopen.com)
  • The authors then propose that the positions of the fluorophores in the membrane can also be deduced from their fluorescence lifetimes (measured with FLIM by using a time-correlated single photon counting system). (biologists.com)
  • This process usually includes at least one step of thermal and/or chemical precipitation at isoelectric point to settle the globulins (also known as larger proteins) further recovered by centrifugation. (pall.com)
  • Previous work had identified three main areas of the trimeric spike proteins of many enveloped viruses like SARS-CoV-2 where modification might improve stabilization and expression. (nature.com)
  • My research area deals with the processing and modification of silk fibroin from Bombyx mori, double layered eggshell membrane, hair keratine, eggshell, crab shell, and adding more to the list for the development of bioscaffold. (nitj.ac.in)
  • To effectively produce these proteins manufacturers will require a process that minimizes potential denaturation effects induced by thermal, chemical, or mechanical stress and prevents carrying over natural contamination such as suspended solids, bioburden, fat and antinutritional factors. (pall.com)
  • The collaboration will capitalize on Velabs' microfluidics-based technology in the field of functional antibody screening. (embl-em.de)
  • The company has the exclusive license of the ADLib® antibody generation technology from RIKEN and has several preclinical and clinical development programs for its therapeutic antibodies. (embl-em.de)
  • Chiome has proprietary antibody generating technology named ADLib® System and has several antibody discovery and preclinical programs. (embl-em.de)
  • Machine learning was used to extract key functional descriptions for protein visualization in the 3D-space, where spatial distributions provide function-based predictive connections between proteins and cancer types. (mit.edu)
  • Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)
  • We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
  • Indeed, recent innovations in technology and methodology have lowered the obstacles that once prevented membrane. (alliedacademies.org)
  • The advancements of NMR have benefited from innovations in technology, resulting in new capabilities as well as enhanced workflows. (bruker.com)